Acorus calamus recycled as an additional carbon source in a microbial fuel cell-constructed wetland for enhanced nitrogen removal.

Acorus calamus was recycled as an additional carbon source in microbial fuel cell-constructed wetlands (MFC-CWs), for efficient nitrogen removal of low carbon wastewater. The pretreatment methods, adding positions, and nitrogen transformations were investigated. Results indicated that alkali-pretreatment cleaved the benzene rings in dominant released organics, producing chemical oxygen demand of 164.5 mg from per gram of A. calamus. Pretreated biomass addition in the anode of MFC-CW attained the maximum total nitrogen removal of 97.6% and power generation of 12.5 mW/m2, which were higher than those with biomass in the cathode (97.6% and 1.6 mW/m2, respectively). However, the duration of a cycle with biomass in the cathode (20-25 days) was longer than that in the anode (10-15 days). Microbial metabolisms related to organics degradation, nitrification, denitrification, and anammox were intensified after biomass recycling. This study provides a promising method to improve nitrogen removal and energy recovery in MFC-CWs.

A novel filter-type constructed wetland for secondary effluent treatment: Performance and its microbial mechanism.

A novel filter-type constructed wetland was constructed by combining plastic fillers and mineral fillers for secondary effluent treatment. Findings showed that TN, TP and COD removal in the constructed wetland with composite fillers (CFCW) was 3.9%, 8.0% and 3.5% higher than that of constructed wetland with ordinary gravel fillers (CW) in the stable phase, respectively. CFCW showed better pollutants removal when dealing with higher influent concentrations and hydraulic loading. The main functional bacteria in two systems were significantly different (p < 0.05). Composite fillers could change the dominant genera, enhance genera activity and increase genera quantity. Denitrification (e.g., Pseudorhodobacter, Zoogloea, Pseudarthrobacter), nitrification (e.g., Devosia, Nitrospira), heterotrophic nitrification-aerobic denitrification (e.g., Paracoccus) and partial denitrification (e.g., g__Simplicispira) in CFCW provided diverse nitrogen metabolism pathways, resulting in higher nitrogen removal. The novel filter-type constructed wetland is suitable for the advanced treatment of sewage treatment plant effluent with enhanced pollutants removal and exuberant microorganisms.

Denitrification performance, bioelectricity generation and microbial response in microbial fuel cell - constructed wetland treating carbon constraint wastewater.

For the deep reduction of nitrogen, the microbial fuel cell-constructed wetland (MFC-CW) was conducted for treating carbon constraint wastewater. Results indicated that nitrogen removal decreased from 94.96% to 24.96% with influent COD/TN (chemical oxygen demand/total nitrogen) from 4 to 0. MFC-CW was seriously affected by low organic wastewater. Wetland plants contributed to denitrification, with TN removal increasing from 46.13% to 64.87%. The bioenergy output showed a linear relationship with influent COD, and the maximum power density of 1.17 mW/m2 was obtained. Correlation analysis indicated that functional genera of Paenibacillus, Trichococcus, norank_KD4-96, norank_OLB14 played a crucial role in nitrogen removal. Influent COD and wetland plants affected carbon and nitrogen metabolisms, and key genes related to denitrification were more abundant in the cathode. Findings illustrated the nitrogen metabolism in MFC-CW with carbon constraint wastewater and will extend the application of MFC-CW in secondary effluent treatment from wastewater treatment plants.