Optimization of factors affecting the rooting of pine wilt disease resistant Masson pine (Pinus massoniana) stem cuttings.

Pine wilt disease (PWD) is a devastating disease affecting trees belonging to the genus Pinus. To control the spread of PWD in the Masson pine forest in China, PWD resistant Masson pine clones have been selected by the Anhui Academy of Forestry. However, because Masson pine is a difficult-to-root species, producing seedlings is challenging, especially from trees older than 5 years of age, which impedes the application of PWD resistant clones. In this study, we investigated the factors affecting rooting of PWD resistant clones and established a cheap, reliable, and simple method that promotes rooting. We tested the effects of three management methods, four substrates, two cutting materials, two cutting treatments, and three collection times on the rooting of cuttings obtained from 9-year-old PWD resistant clones. Rooting was observed only in stem cuttings treated with the full-light automatic spray management method. Additionally, stem cuttings showed a significantly higher rooting rate and root quality than needles cuttings. Compared with other substrates, stem cuttings planted in perlite produced the longest adventitious root and the highest total root length and lateral root number. Moreover, stem cuttings of PWD resistant clones collected in May showed a significantly higher rooting rate and root quality than those collected in June and July. Moreover, stem cuttings prepared with a horizontal cut while retaining the needles showed significantly higher rooting rate and root quality than those prepared with a diagonal cut while partly removing the needles. This study promotes the reproduction of seedlings of PWD-resistant Masson pine clones which helps control the spread of PWD, meanwhile, provides a technical reference for the propagation of mature pine trees via cuttings.

Identification, classification, and characterization of AP2/ERF superfamily genes in Masson pine (Pinus massoniana Lamb.).

Transcription factors (TFs) play crucial regulatory roles in controlling the expression of the target genes in plants. APETALA2/Ethylene-responsive factors (AP2/ERF) are part of a large superfamily of plant-specific TFs whose members are involved in the control of plant metabolism, development and responses to various biotic and abiotic stresses. However, the AP2/ERF superfamily has not been identified systematically in Masson pine (Pinus massoniana), which is one of the most important conifer in southern China. Therefore, we performed systematic identification of the AP2/ERF superfamily using transcriptome sequencing data from Masson pine. In the current study, we obtained 88 members of the AP2/ERF superfamily. All PmAP2/ERF members could be classified into 3 main families, AP2 (7 members), RAV (7 members), ERF (73 members) families, and a soloist protein. Subcellular localization assays suggested that two members of PmAP2/ERF were nuclear proteins. Based on pine wood nematode (PWN) inoculated transcriptome and qPCR analysis, we found that many members of PmAP2/ERF could respond to PWN inoculation and PWN related treatment conditions in vitro. In general, members of the AP2/ERF superfamily play an important role in the response of Masson pine responds to PWN. Furthermore, the roles of the AP2/ERF superfamily in other physiological activities of Masson pine remain to be further studied.

Altered water uptake patterns of Populus deltoides in mixed riparian forest stands.

Plant water uptake plays an important role in regulating ecosystem water balance and its productivity. However, previous studies regarding plant water uptake were primarily conducted in wet areas under seasonal drought conditions, with a limited understanding of the proportion and drivers of plant water uptake under humid conditions. Actually, climate change and variations in global precipitation patterns could simultaneously trigger seasonal drought and flooding. Therefore, it is critical to explore patterns and mechanisms for plant water uptake under humid conditions in wet regions. Here, we employed dual stable isotopes of hydrogen and oxygen coupled with a Bayesian mixing model (MixSIAR) to explore the water uptake patterns of Populus deltoides in two types of riparian forests (pure vs. mixed stand of P. deltoides), under different magnitudes of rainfall (7.9, 15.4 and 34.1 mm), in the Middle-Lower Reaches of the Yangtze River in China. We further used both partial correlation and variation partitioning analyses to determine the relative importance of soil variables and plant properties affecting the proportion of P. deltoides water uptake from different soil layers. Our results revealed that compared to pure stands, P. deltoides in mixed stands had a lower water uptake proportion from deep soil layers (60-80, 80-100 cm) and had higher water uptake from shallow soil layers (0-20, 20-40 cm) under 15.4 mm and 34.1 mm rainfall events. Our results also revealed that plant properties such as leaf biomass, fine root biomass, and diameter at breast height were the primary factors influencing water uptake by P. deltoides. This suggests that P. deltoides in mixed stands could increase the proportion of water uptake from shallow soil layers through altering plant attributes. These findings indicate that mixed stands could restrain frequent extreme rainfall events and subsequent flooding, suggesting more resilience towards future climatic variability.

Higher soil capacity of intercepting heavy rainfall in mixed stands than in pure stands in riparian forests.

Changes in global precipitation patterns would make wet regions more humid and extreme precipitation events occur frequently, followed by widespread flooding. Riparian forests are more capable of withstanding floods than inland forests because they are frequently exposed to short-term flooding events. Although many previous studies have investigated the soil water dynamics of terrestrial forests, little is known about how the soil water of riparian forests responds to different amounts of rainfall and which factors mainly regulate the soil water-holding capacity. Here, we employed stable hydrogen isotope to explore the contribution of different magnitudes of rainfall (7.9, 18.6 and 34.1 mm) to the soil water in two types of riparian forests (pure vs. mixed stand of Populus deltoides) in the middle-lower reaches of the Yangtze River, China. We further used structure equation modelling to determine the relative importance of soil properties and vegetation biomass in affecting the contribution of different magnitudes of rainfall to soil water. Our results revealed that there was no significant difference between these two stand types in the contributions of light and moderate rainfall to soil water, while the contribution of heavy rainfall to soil water (CHRSW) in mixed stand was significantly higher than that in pure stand (74.3% vs. 62.9%), suggesting that mixed stand soil has higher water-holding capacity than pure stand soil. Furthermore, soil properties were the best predictor affecting CHRSW, which explained 68% and 59% of the variation in the CHRSW on the 1st and 8th days after rainfall, respectively. Moreover, the root biomass could indirectly affect the CHRSW. Overall, mixed stand soil had a greater capacity in intercepting and storing rainwater than pure stand soil, implying that the mixed stand plantation, rather than the pure stand, should be recommended in riparian forest restoration projects that aim to improve their capacity for alleviating floods.