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1.
ACS Omega ; 9(27): 29350-29359, 2024 Jul 09.
Artículo en Inglés | MEDLINE | ID: mdl-39005835

RESUMEN

Chemical methods for measuring soil organic content are often slow and yield inaccurate results due to significant errors. Simple summation of components may not accurately determine total organic content. In contrast, fluorescence imaging techniques offer rapid, in situ monitoring without complex pretreatment and demonstrate rapid and accurate assessment of soil organic content. Utilizing a soil organic pollutant fluorescence imaging in situ monitoring system that we independently developed, we conducted laboratory experiments to explore methods for acquiring fluorescence signals of petroleum hydrocarbons in soil and extracting image features. We used this monitoring system to obtain fluorescence images of crude oil in standard soil (soil properties are shown in Table S1) samples at concentrations ranging from 0 to 100 g/kg, and the coefficient of determination of the total amount inversion model reached 0.999. Simultaneously, we applied the system to a deserted petroleum storage area, and the relative standard deviation values of 16 of the 18 groups of tests were less than 1%, indicating that the monitoring system is highly stable when applied in the field. This study provides both theoretical foundation and technical support for the rapid and nondestructive detection of total petroleum hydrocarbons in soil at field sites.

2.
Adv Opt Mater ; 12(12)2024 Apr 24.
Artículo en Inglés | MEDLINE | ID: mdl-38899010

RESUMEN

This paper showcases an experimental demonstration of near-field optical trapping and dynamic manipulation of an individual extracellular vesicle. This is accomplished through the utilization of a plasmonic dielectric nanoantenna designed to support an optical anapole state-a non-radiating optical state resulting from the destructive interference between electric and toroidal dipoles in the far-field, leading to robust near-field enhancement. To further enhance the field intensity associated with the optical anapole state, a plasmonic mirror is incorporated, thereby boosting trapping capabilities. In addition to demonstrating near-field optical trapping, the study achieves dynamic manipulation of extracellular vesicles by harnessing the thermoelectric effect. This effect is induced in the presence of an ionic surfactant, cetyltrimethylammonium chloride (CTAC), combined with plasmonic heating. Furthermore, the thermoelectric effect improves trapping stability by introducing a wide and deep trapping potential. In summary, our hybrid plasmonic-dielectric trapping platform offers a versatile approach for actively transporting, stably trapping, and dynamically manipulating individual extracellular vesicles.

3.
PLoS One ; 19(3): e0300422, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38517877

RESUMEN

To study the propagation pattern of congestion risk in the traffic network and enhance risk control capabilities, a model has been developed. This model takes into account the probabilities of five threats (the risk occurrence probability; the risk of loss; the unpredictability of risk; the uncontrollability of risk; the transferability of risk) in the traffic network to define the risk entropy and determine the risk capacity, analyze the mechanism of congestion risk propagation, and explore the impact of risk resistance, the average degree of risk capacity at intersections, and the degree of correlation on congestion risk propagation. Further, a control method model for risk propagation is proposed. Numerical simulation results demonstrate that the risk resistance parameter θ can inhibit the propagation of congestion risk during traffic congestion. The highest efficiency in controlling risk propagation is achieved when θ reaches a threshold value θ*. Furthermore, the average degree of intersection risk capacity α shows a positive correlation with θ* and a negative correlation with control efficiency. However, the degree of association ω has a negative effect on risk propagation control, decreasing the degree of association between nodes aids in risk propagation control.


Asunto(s)
Entropía , Simulación por Computador
4.
Biotechnol Biofuels Bioprod ; 16(1): 151, 2023 Oct 05.
Artículo en Inglés | MEDLINE | ID: mdl-37798770

RESUMEN

With the invasion of green tides and the increase of urban green areas worldwide, multimillion tons of Enteromorpha need to be reutilized. In this study, Enteromorpha prolifera powder is considered a promising biomass resource for the production of commercial chemical products production. Ilamycins, novel cyclic heptapeptides with significant anti-TB activities, are isolated from Streptomyces atratus SCSIO ZH16, a deep-sea-derived strain. Using EP powder as a nitrogen source, the production of ilamycins reached 709.97 mg/L through optimization of the nitrogen source using the engineered strain S. atratus SCSIO ZH16 ΔR. After mutant strain constructions and tests, strain S. atratus SCSIO ZH16 ΔR::bldD EP powder achieved a higher production titer of ilamycins. Furthermore, the production titer of ilamycins and ilamycin E reached 1561.77 mg/L and 745.44 mg/L, respectively, in a 5 L bioreactor. This study suggests that E. prolifera is a promising and eco-friendly nitrogen source for the production of ilamycins.

5.
Chem Commun (Camb) ; 59(61): 9376-9379, 2023 Jul 27.
Artículo en Inglés | MEDLINE | ID: mdl-37432665

RESUMEN

Commercial TS-1 zeolite was functionalized as a stable catalyst for the one-pot transformation of fructose to methyl lactate (MLA) by the reaction solvent (methanol) to generate increased catalytic activity. As a result, TS-1 was recycled for 14 cycles without a regeneration process by calcination and accompanied by a surprising increased catalytic activity. This work is expected to provide a new option for the industrial production of biomass-based MLA by heterogeneous chemocatalysis.

6.
Life Sci Alliance ; 6(6)2023 06.
Artículo en Inglés | MEDLINE | ID: mdl-37024123

RESUMEN

Although long noncoding RNAs (lncRNAs) experience weaker evolutionary constraints and exhibit lower sequence conservation than coding genes, they can still conserve their features in various aspects. Here, we used multiple approaches to systemically evaluate the conservation between human and mouse lncRNAs from various dimensions including sequences, promoter, global synteny, and local synteny, which led to the identification of 1,731 conserved lncRNAs with 427 high-confidence ones meeting multiple criteria. Conserved lncRNAs, compared with non-conserved ones, generally have longer gene bodies, more exons and transcripts, stronger connections with human diseases, and are more abundant and widespread across different tissues. Transcription factor (TF) profile analysis revealed a significant enrichment of TF types and numbers in the promoters of conserved lncRNAs. We further identified a set of TFs that preferentially bind to conserved lncRNAs and exert stronger regulation on conserved than non-conserved lncRNAs. Our study has reconciled some discrepant interpretations of lncRNA conservation and revealed a new set of transcriptional factors ruling the expression of conserved lncRNAs.


Asunto(s)
ARN Largo no Codificante , Ratones , Humanos , Animales , Secuencia Conservada/genética , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Regulación de la Expresión Génica/genética , Factores de Transcripción/genética , Evolución Biológica
7.
Sci Adv ; 9(7): eabq7750, 2023 02 15.
Artículo en Inglés | MEDLINE | ID: mdl-36791200

RESUMEN

Bone fractures and defects pose serious health-related issues on patients. For clinical therapeutics, synthetic scaffolds have been actively explored to promote critical-sized bone regeneration, and electrical stimulations are recognized as an effective auxiliary to facilitate the process. Here, we develop a three-dimensional (3D) biomimetic scaffold integrated with thin-film silicon (Si)-based microstructures. This Si-based hybrid scaffold not only provides a 3D hierarchical structure for guiding cell growth but also regulates cell behaviors via photo-induced electrical signals. Remotely controlled by infrared illumination, these Si structures electrically modulate membrane potentials and intracellular calcium dynamics of stem cells and potentiate cell proliferation and differentiation. In a rodent model, the Si-integrated scaffold demonstrates improved osteogenesis under optical stimulations. Such a wirelessly powered optoelectronic scaffold eliminates tethered electrical implants and fully degrades in a biological environment. The Si-based 3D scaffold combines topographical and optoelectronic stimuli for effective biological modulations, offering broad potential for biomedicine.


Asunto(s)
Biomimética , Andamios del Tejido , Andamios del Tejido/química , Osteogénesis , Regeneración Ósea , Diferenciación Celular , Impresión Tridimensional , Ingeniería de Tejidos
8.
World J Hepatol ; 15(12): 1258-1271, 2023 Dec 27.
Artículo en Inglés | MEDLINE | ID: mdl-38223416

RESUMEN

Primary liver cancer is a severe and complex disease, leading to 800000 global deaths annually. Emerging evidence suggests that inflammation is one of the critical factors in the development of hepatocellular carcinoma (HCC). Patients with viral hepatitis, alcoholic hepatitis, and steatohepatitis symptoms are at higher risk of developing HCC. However, not all inflammatory factors have a pathogenic function in HCC development. The current study describes the process and mechanism of hepatitis development and its progression to HCC, particularly focusing on viral hepatitis, alcoholic hepatitis, and steatohepatitis. Furthermore, the roles of some essential inflammatory cytokines in HCC progression are described in addition to a summary of future research directions.

9.
Int J Parasitol Parasites Wildl ; 14: 157-160, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33665083

RESUMEN

Anaplasma spp. are important zoonotic tick-borne pathogens that impact on human health. There are few reports on the prevalence and molecular genetic characteristics of Cervidae species in China. The purpose of this study, therefore, was to investigate the presence of Anaplasma spp. in blood samples of Tian Shan wapiti (Cervus elaphus songaricus) in the Xinjiang Uygur Autonomous Region of China, and conduct phylogenetic analyses. A total of 50 blood samples (wild deer n = 26, and captive deer n = 24) were collected from the deer. PCR was used to detect Anaplasma spp. in the blood samples. Forty percent (20) of the samples were found to contain Anaplasma spp. Three Anaplasma species DNA were detected in deer blood samples: A. bovis (n = 13), A. ovis (n = 18), and A. phagocytophilum (n = 11). Among the 20 Anaplasma spp. positive samples, 14 were mixed infection of two or three pathogens. The prevalence of Anaplasma species in wild deer was significantly higher than that of captive deer, 73.1% (19) vs 4.2% (1) respectively, (p < 0.01). Two A. ovis sequence types (AB1, and AB2), three A. ovis sequence types (AO1-AO3), and one A. phagocytophilum sequence type (AP1) were obtained in this study. The sequences of AO1 shared 100% identity with a human isolate from Cyprus. Our results suggest that wild deer are more likely to become infected with Anaplasma spp. than captive individuals, and thus, could potentially transmit pathogens to humans.

10.
Photobiomodul Photomed Laser Surg ; 38(11): 694-702, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33103954

RESUMEN

Objective: We developed an implantable wireless blue micro light-emitting diode (micro-LED) device and evaluated the utility of continuous antimicrobial blue light (aBL) irradiation emitted from this micro-LED for root canal disinfection. Methods: An implantable wireless blue micro-LED device (peak wavelength: 410 nm, maximum power: 15 mW) was developed to be placed in the root canal. Optical transmission of the device in human dentin tissue was simulated using Monte Carlo ray-tracing method. The bactericidal effect of low-level aBL on planktonic root canal infection-related bacteria [Enterococcus faecalis, methicillin-resistant Streptococcus aureus (MRSA), and Prevotella intermedia] was evaluated by colony counting. The biocompatibility of continuous low-level aBL exposure was evaluated by infrared thermal imaging and cell viability tests. Thirty extracted intact human single-rooted teeth were prepared and the root canals were infected with E. faecalis for 14 days to form biofilm. The infected root canals were randomly divided into three groups (n = 10), and treated with normal saline (group NS), calcium hydroxide (group CH), and micro-LED device (group aBL) for 3 and 7 days. The bactericidal effect of each group was evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Results: Monte Carlo simulation showed that blue light irradiation of the micro-LED device decreased exponentially with the light transmission distance through human dentin tissue. Planktonic E. faecalis, MRSA, and P. intermedia were significantly eliminated after irradiation with 432, 36, and 1.35 J/cm2 aBL, respectively (p < 0.05). Infrared thermal imaging and cell viability tests showed that continuous aBL exposure is biocompatible in vitro. CLSM and SEM analyses revealed that the micro-LED device had a greater antimicrobial effect than CH on E. faecalis biofilm in the root canal. Conclusions: The wireless blue micro-LED device is a promising and user-friendly approach for root canal disinfection that will facilitate infection control in the root canal using aBL.


Asunto(s)
Antiinfecciosos , Fotoquimioterapia , Biopelículas , Cavidad Pulpar , Enterococcus faecalis , Humanos
11.
Parasitol Res ; 119(11): 3923-3927, 2020 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-33009948

RESUMEN

The objective of this study was to determine the infection rate and genetic diversity of Cryptosporidium spp. in minks, foxes, and raccoon dogs, farmed in the Xinjiang Uygur Autonomous Region, Northwest China. Fresh fecal specimens were collected from individual cages of farmed minks (n = 214), blue foxes (n = 35), and raccoon dogs (n = 39) and examined using nested PCR based on the Cryptosporidium spp. small subunit rRNA gene. Cryptosporidium spp. was detected in 35 cages (12.2%, 35/288), with a higher infection rate detected in raccoon dogs (20.5%) compared with minks (12.1%) and blue foxes (2.9%). Sequence analysis showed that Cryptosporidium canis was the only species identified in blue foxes and raccoon dogs, while in the 26 Cryptosporidium-positive mink specimens, Cryptosporidium mink genotype (n = 17), C. canis (n = 7), and Cryptosporidium parvum (n = 2) were identified. Further analysis based on the 60-kDa glycoprotein (gp60) gene determined that both C. parvum isolates belonged to the subtype IIdA15G1, while eight of the 17 Cryptosporidium mink genotype isolates were a novel subtype that we have named XeA5G1. To the best of our knowledge, this is the first report of C. parvum subtype IIdA15G1 infection in minks. Since all the Cryptosporidium species/genotypes identified in minks, foxes, and raccoon dogs from Xinjiang have been previously found in humans, our results suggest that these fur animals may play a role in the transmission of zoonotic Cryptosporidium.


Asunto(s)
Criptosporidiosis/parasitología , Cryptosporidium/genética , Zorros/parasitología , Visón/parasitología , Perros Mapache/parasitología , Animales , China , Criptosporidiosis/transmisión , Cryptosporidium/aislamiento & purificación , Granjas , Heces/parasitología , Genotipo , Humanos , Reacción en Cadena de la Polimerasa , ARN Ribosómico 18S/genética
12.
Parasitol Res ; 119(9): 3119-3123, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32743725

RESUMEN

Few reports of Cryptosporidium spp. in snakes in China have been published. To determine the infection rate and document the presence of Cryptosporidium in pet snakes using molecular methods, 273 fecal samples were collected from eight species of pet snakes from 13 pet households in Beijing, China, and were examined by PCR amplification of the small subunit ribosomal RNA gene. Cryptosporidium was detected from 17 of 273 (6.2%) samples, and nine out of 13 households tested positive for Cryptosporidium with a range of 3.3 to 33.3% among households showing significant difference (p < 0.01). The infection rate of Cryptosporidium for females and males was 6.5% (13/201) and 5.6% (4/72), respectively, showing no significant difference (p > 0.05). Six out of eight pet snake species tested positive for Cryptosporidium with a range of 4.2 to 9.1% among species, showing no significant difference (p > 0.05). Two Cryptosporidium species were identified: Cryptosporidium serpentis in 10 samples and Cryptosporidium varanii in seven samples. No zoonotic Cryptosporidium species occur in our study populations.


Asunto(s)
Criptosporidiosis/parasitología , Cryptosporidium/aislamiento & purificación , Mascotas/parasitología , Serpientes/parasitología , Animales , Beijing , Cryptosporidium/clasificación , Cryptosporidium/genética , Heces/parasitología , Reacción en Cadena de la Polimerasa
13.
J Eukaryot Microbiol ; 67(6): 642-647, 2020 11.
Artículo en Inglés | MEDLINE | ID: mdl-32623806

RESUMEN

Blastocystis is a common intestinal protozoan in humans and various animals worldwide. A few studies have reported the genetic characterization of Blastocystis in pigs in China, but no epidemiological data are available from the Xinjiang Uygur Autonomous Region. In this study, 801 fecal samples were collected from seven scale pig farms in Xinjiang and tested by polymerase chain reaction and sequence analysis of the partial SSU rRNA gene. The average infection rate of Blastocystis was 21.7% (174/801), with 7.1% in preweaning piglets, 10.0% in postweaning piglets, 31.8% in fattening pigs, and 41.9% in sows (χ2  = 104.89; P < 0.01). Blastocystis subtypes ST1 (7/174), ST3 (2/174), and ST5 (165/174) were identified, with subtype ST5 being predominant in each of the pig farms and in each of the age groups. ST3 and ST5 were identified in preweaning piglets, and ST1, ST3, and ST5 were identified in postweaning piglets. In contrast, only the subtype ST5 was observed in fattening pigs and sows. Genetic polymorphisms were observed at the intrasubtype level, including two variations of ST1 (ST1A, ST1B), and seven of ST5 (ST5A to ST5G), by sequence alignment analysis and phylogenetic analysis. More studies are needed to elucidate the transmission and public health significance of Blastocystis in pigs in various areas.


Asunto(s)
Infecciones por Blastocystis/veterinaria , Blastocystis/genética , Enfermedades de los Porcinos/parasitología , Porcinos/parasitología , Animales , Blastocystis/clasificación , Blastocystis/aislamiento & purificación , Infecciones por Blastocystis/epidemiología , China/epidemiología , ADN Protozoario , Granjas , Heces/parasitología , Filogenia , Reacción en Cadena de la Polimerasa , Polimorfismo Genético , Enfermedades de los Porcinos/epidemiología
14.
Parasite ; 27: 35, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32410727

RESUMEN

Blastocystis sp. is a ubiquitous protist that has been frequently reported in humans and animals worldwide. A total of 355 fecal samples of experimental rats were collected from four laboratory rearing facilities in China, and Blastocystis sp. was detected by PCR amplification of the partial small subunit ribosomal (SSU) rRNA gene. Twenty-nine (8.2%, 29/355) samples were positive for Blastocystis sp., with the highest infection rate (20.7%, 24/116) in rats of the Zhengzhou1, followed by that in the Zhengzhou2 (5.0%, 2/40), Shenyang (3.0%, 3/100) and Wuhan (0) rearing facilities. Among the three rat strains, Sprague-Dawley (SD) rats had higher infection rates (11.3%, 17/151) compared to Wistar rats (8.7%, 9/104) and spontaneously hypertensive (SH) rats (3.0%, 3/100). Two Blastocystis sp. subtypes (ST4 and ST7) were identified. ST4 was the predominant subtype detected in 26 samples (89.7%). A phylogenetic analysis demonstrated that the sequences of ST4 and ST7 obtained in this study were clustered with their reference subtypes. To our knowledge, this is the first report of Blastocystis sp. in experimental rats in China. Pathogen infections in laboratory animals need to be monitored due to fecal-oral transmission.


TITLE: Identification moléculaire et sous-typage de Blastocystis sp. chez des rats de laboratoire en Chine. ABSTRACT: Blastocystis sp. est un protiste omniprésent qui a été fréquemment signalé chez l'homme et les animaux du monde entier. Un total de 355 échantillons fécaux de rats de laboratoire ont été prélevés dans quatre installations d'élevage en laboratoire en Chine et Blastocystis sp. a été détectée par amplification par PCR du gène partiel de la petite sous-unité de l'ARNr ribosomique (SSU). Vingt-neuf échantillons (8,2 %, 29/355) étaient positifs pour Blastocystis sp., avec le taux d'infection le plus élevé (20,7 %, 24/116) chez les rats des élevages de Zhengzhou1, suivi de ceux de Zhengzhou2 (5,0 %, 2/40), Shenyang (3,0 %, 3/100) et Wuhan (0). Parmi les trois souches de rats, les rats Sprague-Dawley (SD) avaient un taux d'infection plus élevé (11,3 %, 17/151) que les rats Wistar (8,7 %, 9/104) et les rats spontanément hypertendus (SH) (3,0 %, 3/100). Deux sous-types de Blastocystis sp. ont été identifiés, ST4 et ST7. ST4 était le sous-type prédominant, détecté dans 26 échantillons (89,7 %). Une analyse phylogénétique a démontré que les séquences de ST4 et ST7 obtenues dans cette étude étaient groupées avec leurs sous-types de référence. À notre connaissance, il s'agit du premier signalement de Blastocystis sp. chez des rats de laboratoire en Chine. Les infections pathogènes chez les animaux de laboratoire doivent être surveillées en raison de la transmission fécale-orale.


Asunto(s)
Animales de Laboratorio/parasitología , Blastocystis/clasificación , Variación Genética , Filogenia , Animales , Blastocystis/aislamiento & purificación , Infecciones por Blastocystis/parasitología , China , ADN Protozoario/genética , ADN Ribosómico/genética , Heces/parasitología , Femenino , Ratas , Ratas Endogámicas SHR , Ratas Sprague-Dawley , Ratas Wistar
15.
Cell Rep ; 31(8): 107694, 2020 05 26.
Artículo en Inglés | MEDLINE | ID: mdl-32460027

RESUMEN

Aging is an inevitable process that involves profound physiological changes. Long non-coding RNAs (lncRNAs) are emerging as important regulators in various biological processes but are not systemically studied in aging. To provide an organism-wide lncRNA landscape during aging, we conduct comprehensive RNA sequencing (RNA-seq) analyses across the mouse lifespan. Of the 1,675 aging-regulated lncRNAs (AR-lncRNAs) identified, the majority are connected to inflammation-related biological pathways. AR-lncRNAs exhibit high tissue specificity; conversely, those with higher tissue specificity are preferentially regulated during aging. White adipose tissue (WAT) displays the highest number of AR-lncRNAs and develops the most dynamic crosstalk between AR-lncRNA and AR-mRNA during aging. An adipose-enriched AR-lncRNA, lnc-adipoAR1, is negatively correlated with aging, and knocking it down inhibits adipogenesis, phenocopying the compromised adipogenic capacity of aged fat. Our works together reveal AR-lncRNAs as essential components in aging and suggest that although each tissue ages in a distinct manner, WAT is a leading contributor to aging-related health decline.


Asunto(s)
Tejido Adiposo/metabolismo , Envejecimiento/genética , ARN Largo no Codificante/genética , Transcriptoma/genética , Animales , Ratones
16.
Vet Parasitol Reg Stud Reports ; 20: 100406, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-32448523

RESUMEN

Bovine anaplasmosis is caused by a group of obligate intracellular bacteria belonging to the genus Anaplasma, which are transmitted by ticks. This study was conducted to determine the prevalences and molecular characterization of Anaplasma spp. in dairy cattle in the upper reaches of the Tarim River in Xinjiang, China. Using polymerase chain reaction (PCR) and sequencing approaches, DNA of Anaplasma spp. was detected in 16 of 493 (3.2%) blood samples from dairy cattle. Positive rates were 0.2% (1/493), 0.4% (2/493), 0.2% (1/493), 2.4% (12/493) and 2.4% (12/493) for A. bovis, A. ovis, A. phagocytophilum like strain, A. phagocytophilum and A. platys like strain, respectively. Anaplasma phagocytophilum and A. platys like strain co-infection was detected in 12 samples. To our knowledge, this is the first report of A. ovis infection in dairy cattle in Xinjiang. This study provides new data on the prevalences of Anaplasma spp. in cattle in Xinjiang, which will help to formulate appropriate control strategies for these pathogens in this area.


Asunto(s)
Anaplasma/aislamiento & purificación , Anaplasmosis/epidemiología , Enfermedades de los Bovinos/epidemiología , Anaplasma/clasificación , Anaplasma/genética , Anaplasmosis/microbiología , Animales , Bovinos , Enfermedades de los Bovinos/microbiología , China/epidemiología , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Análisis de Secuencia de ADN/veterinaria , Especificidad de la Especie
17.
Exp Parasitol ; 210: 107850, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32027893

RESUMEN

Experimental rats are important animal models, and a history of pathogenic infections in these animals will directly affect the animal trial results. Enterocytozoon bieneusi is a ubiquitous potential pathogen transmitted via the fecal-oral route. To determine the prevalence and genotypic distributions of E. bieneusi in experimental rats in China, 291 fresh fecal samples were collected from four medical experimental animal centers. Enterocytozoon bieneusi was screened via nested-PCR amplification of the internal transcribed spacer (ITS) region. Of the rats tested, 4.8% (14/291) were positive for E. bieneusi. Five E. bieneusi ITS genotypes (four known: EbpA, EbpC, CHY1, and N; one novel: SHR1) were detected among 14 sequenced samples. The dominant E. bieneusi genotype was EbpA (50.0%, 7/14). In the phylogenetic analysis, genotypes EbpA and EbpC belonged to the previously described group 1, genotypes N and SHR1 belonged to group 2, and genotype CHY1 belonged to the novel group 12. To our knowledge, this is the first report of E. bieneusi in experimental laboratory rats in China. Infections with this pathogen must be monitored in laboratory animals, and quality control officers in the medical experimental centers should attempt to trace the pathogen's source and stop its transmission.


Asunto(s)
Enterocytozoon/aislamiento & purificación , Microsporidiosis/microbiología , Animales , China/epidemiología , ADN de Hongos/aislamiento & purificación , Enterocytozoon/clasificación , Enterocytozoon/genética , Heces/microbiología , Femenino , Genotipo , Técnicas de Genotipaje , Microsporidiosis/epidemiología , Filogenia , Reacción en Cadena de la Polimerasa , Ratas , Ratas Endogámicas SHR , Ratas Sprague-Dawley , Ratas Wistar
18.
Lasers Dent Sci ; 4(1): 25-32, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-33907707

RESUMEN

PURPOSE: Inferior alveolar nerve (IAN) injury is one of the most serious complications after extraction of impacted lower third molars. Photobiomodulation (PBM) therapy has been noted to reduce pain and inflammation while promoting tissue healing. This study examined the efficacy of PBM therapy tested in a case series of patients with postoperative IAN injury. MATERIAL AND METHODS: 20 patients with post-extraction IAN injury were involved in this study and divided into two groups. In the study group, PBM therapy (808-nm laser, 16 mW, 3 J/cm2) was used every other day for 2 weeks solely on post-extraction sockets in 10 patients diagnosed with IAN injury. In the control group, mecobalamine was prescribed to 10 patients with IAN injury. Objective and subjective recovery of IAN paresthesia was evaluated using clinical neurosensory testing and visual analog score. RESULTS: All patients showed improvement in both objective and subjective examination. Notably, the visual analog score was significantly improved after PBM treatment compared to the mecobalamine treatment (p < 0.05). CONCLUSION: PBM therapy with 808-nm laser appears to be an effective approach to manage paresthesia post-IAN injury following impacted third molar surgery. Given the limited sample size in this study, large-scale, placebo-controlled, multi-center randomized controlled trials are needed for further validation of this innovative treatment.

19.
Front Microbiol ; 10: 2401, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31695688

RESUMEN

Enterocytozoon bieneusi is an obligate intracellular fungus, infecting various invertebrate and vertebrate hosts, it is common in humans and causes diarrhea in the immunocompromised. In the present study, 801 fecal specimens were collected from pigs on seven large-scale pig farms in Xinjiang, China. Nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) gene showed that the overall E. bieneusi infection rate was 48.6% (389/801). The E. bieneusi infection rates differed significantly among the collection sites (20.0-73.0%) (χ2 = 75.720, df = 6, p < 0.01). Post-weaned pigs had the highest infection rate (77.2%, 217/281), followed by fattening pigs (67.4%, 87/129) and pre-weaned suckling pigs (35.5%, 60/169). Adult pigs had the lowest infection rate (11.3%, 25/222). The E. bieneusi infection rates also differed significantly among age groups (χ2 = 246.015, df = 3, p < 0.01). Fifteen genotypes were identified, including 13 known genotypes (CHC, CS-1, CS-4, CS-7, CS-9, D, EbpA, EbpC, EbpD, H, PigEb4, PigEBITS5, and WildBoar8) and two novel genotypes (XJP-II and XJP-III). Among them, six genotypes (CS-4, D, EbpA, EbpC, H, and PigEBITS5) have been reported in humans. Phylogenetic analysis showed that all the genotypes belonged to Group 1 of E. bieneusi. These findings suggest that pigs may play an important role in transmitting E. bieneusi infections to humans.

20.
Mater Sci Eng C Mater Biol Appl ; 98: 134-139, 2019 May.
Artículo en Inglés | MEDLINE | ID: mdl-30813013

RESUMEN

In clinic infection is the paramount cause for failure of guided bone regeneration (GBR) membranes. Therefore, it is crucial to develop anti-infective GBR membranes for clinical bone repair application. In this research, we successfully prepared electrospun core-shell nanofibers loaded with metronidazole (MNA) and nano-hydroxyapatites (nHA), which could be employed for anti-infective GBR membranes due to the achievement of dual functions with enhanced osteogenesis and slow MNA release. The nanofiber shell was composed of polycaprolactone and nHA, whilst the nanofiber core was gelatin and MNA. The MNA release and cell proliferation experiments showed that compared with directly MNA-loaded nanofibers, the core-shell nanofibers possessed slower MNA release profile, which resulted in the decrease in cytotoxicity of MNA to bone mesenchymal stem cells. The osteogenic measurements demonstrated that the core-shell nanofibers could enhance bone formation. Additionally, the anti-bacterial experiments indicated that the core-shell nanofibers could prevent colonization of anaerobic bacteria. In summary, the results in the present study revealed the potential of the core-shell electrospun nanofibers with dual functions of enhanced osteogenesis and anti-infection for optimal clinical application as GBR membranes.


Asunto(s)
Antiinfecciosos/química , Antiinfecciosos/farmacología , Nanofibras/química , Andamios del Tejido/química , Regeneración Ósea/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Humanos , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/efectos de los fármacos , Metronidazol/química , Poliésteres/química
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