Cobalt germanium hydroxides with asymmetric electron distribution and surface hydroxyl groups for superb catalytic degradation performances.

Peroxymonosulfate (PMS)-based advanced oxidation processes (AOPs) are attractive approaches for solving the global problem of water pollution, due to the generation of highly-active reactive oxygen species (ROS). Therefore, highly-efficient PMS activation is crucial for promoting the catalytic degradation of environmental pollutants. Here, bimetallic CoGeO2(OH)2 nanosheets with abundant surface hydroxyl groups (CGH) were synthesized via a simple hydrothermal route for PMS activation and degradation of various organic contaminants for the first time. The abundant surface hydroxyl groups (≡Co-OH/≡Ge-OH) could promptly initiate PMS to generate highly-active species: singlet oxygen (1O2), sulfate radicals (SO4·-) and hydroxyl radicals (HO•), while the asymmetric electron distribution among Co-O-Ge bonds derived from the higher electronegativity of Ge than Co further enhances the quick electron transfer to promote the redox cycle of Co2+/Co3+ and Ge2+/Ge4+, thereby achieving an outstanding catalytic capability. The optimal catalyst exhibits nearly 100 % catalytic degradation performance of dyes (Methylene blue, Rhodamine B, Methyl orange, Orange II, Methyl green) and antibiotics (Norfloxacin, Bisphenol A, Tetracycline) over a wide pH range of 3-11 and under different coexisting anion conditions (Cl-, HCO3-, NO3-, HA), suggesting the excellent adaptability for practical usage. This study could potentially lead to novel perspectives on the remediation of water areas such as groundwater and deep-water areas.

Fluorescence immunosensor based on a specific monoclonal antibody for highly sensitive and rapid detection of gizzerosine in feed.

Gizzerosine is a biogenic amine produced in fish meal drying process and posted higher mortality due to gizzard erosion in poultry than histamine. However, it is difficult to obtain gizzerosine and achieve sensitive practical detection due to its simple structure. Herein, a monoclonal antibody (mAb) specific to gizzerosine was generated based on the new structural design and a fluorescence immunosensor for sensitive and on-site detection of gizzerosine in feed was first established. Molecular modeling of the three-dimensional (3D) structure and surface electrostatic potential of gizzerosine indicated that the carbonyl group of gizzerosine hapten might affect the important sites of antigen-antibody interactions. The proposed structure was used to obtain the sensitive and specific mAb with IC50 of 3.88 ng/mL in indirect competitive ELISA which was approximately 100-fold lower than that of direct competitive ELISA. Considering the practical application scenarios, a fluorescence immunosensor based on microporous dry method integrated with independent quality control line was established to improve detection stability. Under the optimum conditions, the proposed immunosensor showed a good linear relationship from 1.10 to 19.78 ng/mL and provided a low detection limit of 50 ng/g which was approximately 80-fold lower than the maximum recommended amount (0.4 mg/kg) of gizzerosine in feed. The recoveries of 6 kinds of feed ranged from 83.1 % to 114.3 %, which was in good consistence with that of UHPLC-MS/MS. Overall, this work provides a fast, cost-effective and reliable on-site tool for rapid screening of gizzerosine residues in feed samples.

Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives.

Gizzerosine is responsible for gizzard erosion and black vomit, owing to excessive gastric acid secretion in poultry. It is a biogenic amine that forms during feed processing. Gizzerosine, a derivative of histamine, is a serious threat to animal feed safety and poultry production because it is more potent after ingestion and more harmful to poultry than histamine. The difficulty of obtaining gizzerosine and the lack of simple, rapid, and sensitive in vitro detection techniques have hindered studies on the effects of gizzerosine on gizzard health and poultry production. In this review, we evaluated the natural formation and the chemical synthesis methods of gizzerosine and introduced seven detection methods and their principles for analyzing gizzerosine. This review summarizes the issues of gizzerosine research and suggests methods for the future development of gizzerosine detection methods.

Deep learning strategies with CReToNeXt-YOLOv5 for advanced pig face emotion detection.

This study underscores the paramount importance of facial expressions in pigs, serving as a sophisticated mode of communication to gauge their emotions, physical well-being, and intentions. Given the inherent challenges in deciphering such expressions due to pigs' rudimentary facial muscle structure, we introduced an avant-garde pig facial expression recognition model named CReToNeXt-YOLOv5. The proposed model encompasses several refinements tailored for heightened accuracy and adeptness in detection. Primarily, the transition from the CIOU to the EIOU loss function optimized the training dynamics, leading to precision-driven regression outcomes. Furthermore, the incorporation of the Coordinate Attention mechanism accentuated the model's sensitivity to intricate expression features. A significant innovation was the integration of the CReToNeXt module, fortifying the model's prowess in discerning nuanced expressions. Efficacy trials revealed that CReToNeXt-YOLOv5 clinched a mean average precision (mAP) of 89.4%, marking a substantial enhancement by 6.7% relative to the foundational YOLOv5. Crucially, this advancement holds profound implications for animal welfare monitoring and research, as our findings underscore the model's capacity to revolutionize the accuracy of pig facial expression recognition, paving the way for more humane and informed livestock management practices.

Factors Influencing The Language Development of Preterm Infants and Their Intervention Strategies / 生物化学与生物物理进展

Preterm infants, born before 37 weeks of gestation, represent a significant portion of newborns globally, many of whom experiencing long-term neurodevelopmental disorders. Language development anomalies are common among preterm infants, often leading to deficits in vocabulary, grammar, phonetics, and semantics, which can persist into adolescence and adulthood. Given these complexities, these developmental challenges necessitate a deeper understanding of the influencing factors and the importance of early intervention. Biological factors such as the degree of prematurity, birth weight, and gender significantly impact language development. Specifically, shorter gestational age and lower birth weight are associated with language difficulties, manifesting in restricted vocabulary, syntax, and grammatical complexity. In addition, the severity of neonatal illnesses, including intracranial hemorrhage, hypoxic-ischemic encephalopathy, and bronchopulmonary dysplasia, critically impact cognitive and language development. Equally important, sensory systems, particularly vision and hearing, are also crucial for language acquisition, for example, retinopathy of prematurity (ROP) may increase the risk of language disorders. Environmental factors also play a vital role in language development of preterm infants. The environment within neonatal intensive care units (NICU), while important for the survival of preterm infants, can inadvertently impose sensory challenges, thereby influencing neurodevelopmental outcomes, including language skills. Beyond the NICU environment, the domestic setting and familial interactions emerge as crucial determinants. Variables such as the parental educational background and socioeconomic status substantially influence the extent and quality of language exposure, thus shaping the linguistic development of preterm infants. Addressing these challenges requires comprehensive early intervention strategies. This includes deploying a range of early evaluation tools, encompassing standardized language development scales and observational techniques, to promptly identify infants at risk of language delays. Recent advances in non-invasive brain imaging techniques, such as event-related potentials and functional magnetic resonance imaging (MRI), have opened new horizons in early detection and intervention planning, providing critical insights into the neurodevelopmental status of these infants. Intervention strategies are diverse and integrate physiological and neurological approaches, environmental modifications, and family-centric practices. Physiologically, addressing sensory impairments and nutritional needs is fundamental to fostering robust language development. This involves interventions like sensory stimulation therapies and nutritional supplements rich in essential brain-development nutrients. Additionally, environmental optimization, particularly in NICU settings, to replicate the protective conditions of womb is crucial for enhancing language learning. Strategies include controlled auditory and visual stimulation and implementing developmental care models. Furthermore, family involvement is equally important. Encouraging active parental engagement and fostering language-enriched interactions are crucial. Notably, innovative approaches such as music therapy have shown promise in enhancing auditory processing and language skills. These interventions utilize the infant brain’s neuroplasticity, combining auditory stimulation with social interaction, thereby enriching the developmental environment for preterm infants. In summary, the language development in preterm infants is shaped by an intricate interplay of biological and environmental factors, requiring a multifaceted and early intervention approach. As our understanding evolves, the integration of medical, educational, and social services will be critical in providing holistic support for the healthy development of these infants. Future research efforts should aim to elucidate the underlying mechanisms of language development in preterm infants and to refine intervention strategies to ensure more effective long-term outcomes.

Simultaneous Solar-Thermal Desalination and Catalytic Degradation of Wastewater Containing Both Salt Ions and Organic Contaminants.

Although solar steam generation is promising in generating clean water by desalinating seawater, it is powerless to totally degrade organic contaminants in the seawater. Herein, solar steam generation and catalytic degradation are integrated to generate clean water by simultaneous solar-driven desalination and catalytic degradation of wastewater containing both salt ions and organic contaminants. Stepwise decoration of three-dimensional nickel foam with polypyrrole, reduced graphene oxide (RGO), and cobalt phosphate is realized to obtain polypyrrole/RGO/cobalt phosphate/nickel foam (PGCN) hybrids for solar-driven desalination and catalytic degradation of wastewater containing antibiotics and salt ions. The oxygen-containing groups of the RGO integrated with the porous nickel foam make the porous PGCN hybrid hydrophilic and ensure the upward transport of water to the evaporation surface, and the oxygen vacancies of the cobalt phosphate allow the PGCN to generate abundant highly active singlet oxygen that could still exhibit excellent catalytic degradation performances in the high salinity and highly alkaline environment of seawater. In addition to the high solar light absorbance and satisfactory solar-thermal conversion efficiency of polypyrrole and RGO, the thermally conductive nickel foam skeleton can effectively transfer the heat generated by the solar-thermal energy conversion to the adjacent cobalt phosphate catalyst and nearby wastewater, achieving a solar-thermal-promoted catalytic degradation of organic contaminants. Therefore, a high pure water evaporation rate of 2.08 kg m-2 h-1 under 1 sun irradiation and 100% catalytic degradation of Norfloxacin and dyes are achieved. The PGCN hybrid is highly efficient in purifying seawater containing 10 ppm Norfloxacin and simultaneously achieves a high purification efficiency of 100 kg m-2 h-1.

Hydrothermally Modified 3D Porous Loofah Sponges with MoS2 Sheets and Carbon Particles for Efficient Solar Steam Generation and Seawater Desalination.

Although the emerging interfacial solar steam generation technology is sustainable and eco-friendly for generating clean water by desalinating seawater and purifying wastewaters, salt deposition on the evaporation surface during solar-driven evaporation severely degrades the purification performances and adversely affect the long-term performance stability of solar steam generation devices. Herein, to construct solar steam generators for efficient solar steam generation and seawater desalination, three-dimensional (3D) natural loofah sponges with both macropores of the sponge and microchannels of the loofah fibers are hydrothermally decorated with molybdenum disulfide (MoS2) sheets and carbon particles. Benefiting from fast upward transport of water, rapid steam extraction, and effective salt-resistant capacity, the 3D hydrothermally decorated loofah sponge with MoS2 sheets and carbon particles (HLMC) with an exposed height of 4 cm can not only obtain heat by its top surface under the downward solar light irradiation based on the solar-thermal energy conversion but also gain environmental energy by its porous sidewall surface, achieving a competitive water evaporation rate of 3.45 kg m-2 h-1 under 1 sun irradiation. Additionally, the 3D HLMC evaporator exhibits long-term desalination stability during the solar-driven desalination of an aqueous salt solution with 3.5 wt % NaCl for 120 h without apparent salt deposition because of its dual type of pores and uneven structure distribution.

Omnidirectionally irradiated three-dimensional molybdenum disulfide decorated hydrothermal pinecone evaporator for solar-thermal evaporation and photocatalytic degradation of wastewaters.

Although most solar steam generation devices are effective in desalinating seawater and purifying wastewaters with heavy metal ions, they are ineffective in degrading organic pollutants from wastewaters. Herein, we design novel solar-driven water purification devices by decoration of three-dimensional pinecones with MoS2 nanoflowers via a one-step hydrothermal synthesis for generating clean water. The vertically arrayed channels in the central rachis and the unique helically arranged scales of the hydrothermal pinecone can not only transfer bulk water upward to the evaporation surface, but also absorb more solar light from different incident angles for solar-thermal evaporation and photodegradation of wastewaters under omnidirectional irradiations. The decorated MoS2 nanoflowers can not only enhance the solar-thermal energy conversion efficiency, but also decompose organic pollutants in the bulk water by their photocatalytic degradation effects. The resultant hydrothermal pinecone with in situ decorated MoS2 (HPM) evaporator exhibits a high evaporation rate of 1.85 kg m-2 h-1 under 1-sun irradiation with a high energy efficiency of 96 %. During the solar-driven water purification processes, the powdery HPM can also photodegrade organic pollutants of methylene blue and rhodamine B with high removal efficiencies of 96 % and 95 %, respectively. For practical demonstration, by floating in the methylene blue solution under 1-sun irradiation, the bulky HPM can generate clean water by simultaneous solar-thermal evaporation and photocatalytic degradation. The integration of solar steam generation and photocatalytic degradation mechanisms makes the HPM evaporator highly promising for practical high-yield purification of wastewaters.

The interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages.

The discovery of STING-related innate immunity has recently provided a deep mechanistic understanding of immunopathy. While the detrimental effects of STING during sepsis had been well documented, the exact mechanism by which STING causes lethal sepsis remains obscure. Through single-cell RNA sequence, genetic approaches, and mass spectrometry, we demonstrate that STING promotes sepsis-induced multiple organ injury by inducing macrophage ferroptosis in a cGAS- and interferon-independent manner. Mechanistically, Q237, E316, and S322 in the CBD domain of STING are critical binding sites for the interaction with the coiled-coil domain of NCOA4. Their interaction not only triggers ferritinophagy-mediated ferroptosis, but also maintains the stability of STING dimers leading to enhanced inflammatory response, and reduces the nuclear localization of NCOA4, which impairs the transcription factor coregulator function of NCOA4. Meanwhile, we identified HET0016 by high throughput screening, a selective 20-HETE synthase inhibitor, decreased STING-induced ferroptosis in peripheral blood mononuclear cells from patients with sepsis and mortality in septic mice model. Our findings uncover a novel mechanism by which the interaction between STING and NCOA4 regulates innate immune response and ferroptosis, which can be reversed by HET0016, providing mechanistic and promising targets insights into sepsis.

Identification of hub genes in response to valproate treatment in patients with epilepsy / 中国药理学通报

Aim To investigate the hub genes associated with response to valproate treatment in patients with epilepsy by using weighted gene co-expression network analysis.Methods We downloaded data from the GEO database and constructed the gene co-expression network.Pearson correlation test was used to calculate the correlation between module genes and clinical traits, to screen gene modules significantly associated with response to valproate treatment, and to screen hub genes according to the connectivity within modules.GO functional enrichment analysis and KEGG pathway analysis were used to annotate the functions of the modules.Results A total of 12 gene co-expression modules were constructed from the correlations of gene expression, in which the yellow module was significantly correlated with the drug treatment(r=0.57, P<0.000 1)and the blue module was significantly correlated with the response to valproate(r=-0.53, P<0.000 1).We found that S1PR5, SARM1 and MAGED1, FBXO31 were in the hub of the co-expression network.The biological annotation function revealed that the genes in both modules were mainly enriched in immune response and MPAK pathways.Conclusions Our work delivers preliminary data that valproate treatment causes the changes of immune and metabolic pathways in patients, and the response to epilepsy may be related to the expression of MAGED1, FBXO31.

Brief Analysis of the ARIMA model on the COVID-19 in Italy

Coronavirus disease 2019 (COVID-19) has been considered as a global threat infectious disease, and various mathematical models are being used to conduct multiple studies to analyze and predict the evolution of this epidemic. We statistically analyze the epidemic data from February 24 to March 30, 2020 in Italy, and proposes a simple time series analysis model based on the Auto Regressive Integrated Moving Average (ARIMA). The cumulative number of newly diagnosed and newly diagnosed patients in Italy is preprocessed and can be used to predict the spread of the Italian COVID-19 epidemic. The conclusion is that an inflection point is expected to occur in Italy in early April, and some reliable points are put forward for the inflection point of the epidemic: strengthen regional isolation and protection, do a good job of personal hygiene, and quickly treat the team leaders existing medical forces. It is hoped that the "City Closure" decree issued by the Italian government will go in the right direction, because this is the only way to curb the epidemic.

Effects of different drying conditions on protein in Cordyceps / 中国中药杂志

In this study,the protein in different Cordyceps samples,which include fresh sample( S1),22 ℃ drying sample( S2),37 ℃ drying sample( S3) and 60 ℃ drying sample( S4),were analyzed by sodium dodecylsupinate-polyacrylamide gel electrophoresis( SDS-PAGE) and two-dimensional electrophoresis( 2-DE). The total protein contents in Cordyceps samples were from 1. 655-4. 493 mg·g~(-1) and the protein contents in fresh sample was the highest. The results of SDS-PAGE showed that the mainly ranges of protein molecular weight of Cordyces samples were 10-100 kDa and the numbers of protein bands were 28 to 41,the fresh sample had the maximum number of protein bands. The 2-DE profiles were analyzed by PDQuest software. The resulted indicated that 488-876 protein spots were detected in different Cordyceps samples and the isoelectric point( pI) was distributed between 4. 5 and 6. 5,the protein molecular weight was distributed in 10-20 kDa and 25-100 kDa,the fresh sample had the maximum number of protein spots. Therefore,the drying process could decrease contents and species of protein in Cordyceps,and the different drying conditions had different effects on protein. These results provide a reference for improving the drying process of Cordyceps.

Synthesis and Release of Dapsone-Alginate (DS-Alg) Conjugate / 中国药学杂志

OBJECTIVE: To synthesize and characterize dapsone-alginate acid (DS-ALG) conjugate. METHODS: Alginate (Alg) was selected as the drug carrier and valine (Val) as the linking arm to synthesize DS-Alg, which could be applied to topical administration. And the synthetic condition of DS-Alg was optimized by changing the amount of 1-(3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), while the pH of solvent was changed in the range of 4.0 to 6.0. The structures of the products were characterized by 1H-NMR, MS and FT-IR. Meanwhile, the drug release in vitro of DS-Alg was investigated in the mixture of pH 7.4, 0.05 mol•L -1 PBS and ethanol by diffusion cells. The concentration of DS or valine-dapsone in the release medium was detected by HPLC. Taking rats with local scald as model, the drug release in vivo was measured by coating the trauma with DS-Alg conjugate cream and monitoring the drug concentration in blood. RESULTS: The optimum synthetic conditions of DS-Alg were as follows: 0.277 g valine-dapsone, 0.400 g sodium alginate, 7 eq EDC, 3 eq NHS, pH of the solvent of 5.5. And during 72 h, there was no DS detected in the release medium or rat plasma. The AUC0→72 h of DS-Alg was 0. It suggested that DS immobilized by Alg with covalent bond was too stable to be released from Alg in vitro and in vivo. The DS-Alg conjugate could effectively prevent DS from entering the systemic circulation. CONCLUSION: DS-Alg conjugate is successfully synthesized. The conjugate is stable that DS cannot be released from the conjugate to the bloodstream, which can efficiently decrease the side effects of DS and has the potential for topical administration.

Application of computer color matching technology in color simulation of Ruyi Jinhuang Powder placebo / 中草药

Objective To study the color of Ruyi Jinhuang Powder (RJP) placebo by using computer color matching technology and achieve fast preparation of RJP placebo. This work provided a new method to simulate the color of Chinese medicine compound placebo. Methods Using the RJP placebo as an example, computer color matching technology was used to establish a mathematical model to correlate the placebo color parameter L, a*, b* value and the colorant concentration. For a measured medicine compound color, the placebo colorant concentration can then be calculated from the model through data fitting and Newton iterative method. The model accuracy was validated using the color comprehensive evaluation index (ΔE). Results The color parameter L, a*, b* of RJP placebo was 68.302 5, 4.079 5, and 34.484 0. The mass fraction of lemon yellow, amaranth, and blue was 0.837 3%, 0.045 8%, and 0.008 5%. The ΔE value between RJP and its placebo was 2.750 0 ± 0.353 6, and there was no obvious visual difference between the medicine compound and its placebo. Conclusion Computer color matching technology can be used to simulate the color of RJP placebo, and can be widely used in the preparation of Chinese medicine placebo.

A lipophilic prodrug of Danshensu: preparation, characterization, and in vitro and in vivo evaluation / 中国天然药物

Danshensu [3-(3, 4-dihydroxyphenyl) lactic acid, DSS], one of the significant cardioprotective components, is extracted from the root of Salvia miltiorrhiza. In the present study, an ester prodrug of Danshensu (DSS), palmitoyl Danshensu (PDSS), was synthesized with the aim to improve its oral bioavailability and prolong its half-life. The in vitro experiments were carried out to evaluate the physicochemical properties and stability of PDSS. Although the solubility of PDSS in water was only 0.055 mg·mL, its solubility in FaSSIF and FeSSIF reached 4.68 and 9.08 mg·mL, respectively. Octanol-water partition coefficient (log P) was increased from -2.48 of DSS to 1.90 of PDSS. PDSS was relatively stable in the aqueous solution in pH range from 5.6 to 7.4. Furthermore, the pharmacokinetics in rats was evaluated after oral administration of PDSS and DSS. AUC and t of PDSS were enhanced up to 9.8-fold and 2.2-fold, respectively, compared to that of DSS. C was 1.67 ± 0.11 μg·mL for PDSS and 0.81 ± 0.06 μg·mL for DSS. Thus, these results demonstrated that PDSS had much higher oral bioavailability and longer circulation time than its parent drug.

Evaluation for extraction process of Shenqi Tongmai Yizhi particles based on antioxidant capacity in vitro and its spectrum-effect relation / 中国中药杂志

Based on DPPH method, the antioxidant activities of Shenqi Tongmai Yizhi particles with different extraction processes were compared. The contribution to the anti-oxidant capacity in vitro was explored by means of grey relational analysis on different chemical compositions in the fingerprint. The results showed that the IC₅₀ concentration values of water extract, water extract from alcohol precipitation, alcohol extract, and alcohol and water extract were 0.801 4, 0.859 1, 0.796 1, 0.918 0 g•L⁻¹; and the alcohol extract is the best method to extract antioxidative components, with the highest antioxidant activity and lowest IC₅₀. When the mass concentration of the herbs reached a certain degree, its free radical clearance rate was similar to that of vitamin C control group. The order of different chemical contributions of constituents to the antioxidant activity in the fingerprint was 4>3>33>53>9>10>11>34>15>59>8>61>52>20>42>18>29. The preliminary exploration for the spectrum efficiency relations provides reference for studying traditional Chinese medicine compound processing method and the pharmacodyamic material basis.

Regeneration of Carthamus tinctorius from Jimsar / 中草药·英文版

Objective: To investigate the factors significantly affecting the shoot buds regeneration of Carthamus tinctorius from Jimsar (CTJ). Methods: Through tissue culture experiments, the influences of culture temperature, illuminance, relative humidity, explants type, seedlings age, and plant growth regulators added in culture media on in vitro regeneration of CTJ were investigated. Results: The culture temperature was set at 24 °C in daytime and 16 °C at night, illuminance at 9000 lx, and 60% relative humidity were suitable for CTJ regeneration. Cotyledons excised from 6-8 d old seedlings were more responsive than even-aged euphyll, hypocotyl, and root explants because of inducing adventitious buds. The highest percentage of regenerated shoots (79.1%) with about five adventitious buds per responding explant was obtained from MS basal medium containing 12.0 mg/L TDZ, 2.5 mg/L IBA, and 1.5 mg/L 2-ip (No. 14 medium). Regenerated shoot buds (80%) could elongate successfully after 1-2 weeks transferred to shoot elongation medium. Conclusion: Suitable factors of shoot buds regeneration for CTJ were determined. It may also make a useful reference for regeneration researches of other C. tinctorius varieties in China. © 2014 Tianjin Press of Chinese Herbal Medicines.

Effects of carbon components of fine particulate matter (PM2.5) on atherogenic index of plasma / 中华预防医学杂志

<p><b>OBJECTIVE</b>To evaluate associations between carbon constituents of fine particulate matter (PM2.5) and atherogenic index of plasma (AIP).</p><p><b>METHODS</b>We collected subjects from two communities by a system sampling, and 112 people aged over 60 years old without cardiovascular disease were recruited. The levels of cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) of objects, and personal exposure to PM2.5 were measured on December, 2011. Total carbon (TC), organic carbon (OC) and elemental carbon (EC) of PM2.5 were detected and AIP was calculated according to its definition.</p><p><b>RESULTS</b>The value of AIP among the 112 subjects was 0.05 ± 0.26. Personal exposure concentration of PM2.5 and its carbon components (TC,OC and EC) were (164.75 ± 110.67), (53.86 ± 29.65), (44.93 ± 26.37) and (9.49 ± 5.75) µg/m(3), respectively. The Pearson analysis showed the linear relationship between TC,OC,EC and AIP, all significant positive correlations. The correlation coefficients were TC (r = 0.307, P < 0.05),OC (r = 0.287, P < 0.05) and EC (r = 0.252, P < 0.05), respectively. The multiple logistic regression analysis showed that when the AIP risk categories were selected as dependent variable and low risk group as reference group, the regression coefficient of TC,OC and EC was separately 1.03 (95%CI:1.01-1.05), 1.03 (95%CI:1.01-1.05), 1.12 (95%CI:1.02-1.22) in the high risk group; while there was no statistical significance of the regression coefficient and OR in the middle risk group.</p><p><b>CONCLUSION</b>There was stable associations between the carbon constituents (TC,OC and EC) of fine Particulate Matter (PM2.5) and AIP. The findings suggested that carbon components of PM2.5 should be considered as risk factors of atherogenic.</p>

Determination of ellagic acid, flavonoids and goshonoside-F5 in Rubi Fructus by HPLC / 中国中药杂志

High-performance liquid chromatographic coupled with variable wavelength detection (HPLC-VWD) has been developed for simultaneous determination of 5 analytes including ellagic acid, quercetin, kaempferol-3-O-beta-D-rutinoside, tiliroside and kaempferol, and high-performance liquid chromatographic with an evaporative light scattering detector (HPLC-ELSD) has been established to determine goshonoside-F5 in extract of Rubi Fructus. Chromatographic separations were carried out on an Agilent ZORBAX SB-C18 column (4.6 mm x 250 mm, 5.0 microm). All calibration curves of reference standards revealed good linearity (R2 > 0.999 5) within the concentration ranges tested. The method limits of detection ranged 0.297-90.144 ng and the method limits ofquantitation ranged 0.990-300.480 ng, respectively. Recoveries of 6 analytes were from 97.11% to 101.7%, with RSD less than 2.1%. The result shows that amounts of the 6 analytes in the samples from 16 localities were found to be different. The higher latitude of growing environment, the more ellagic acid in herb. The content of total flavonoids in sample from east localities were higher than that in middle and west localities, and the content of goshonoside-F5 in Bozhou, Anhui province was higher than others. This method was found to be simple, accurate, sensitive with good repeatability. Those results might serve as a sound foundation for further study, quality control and application of Rubi Fructus.

Chemical constituents in Akebiae Fructus based on UFLC-Q-TOF/MS / 中草药

Objective: To identify the chemical constituents in Akebiae Fructus by UFLC-Q-TOF/MS method. Methods: The separation was performed on an Acquity UPLC BEH C18 Column (100 mm × 2.1 mm, 1.7 μm), with a mobile phase using 0.1% formic acid-acetonitrile and water containing 0.1% formic acid (B) for gradient elution. The flow rate was 0.3 mL/min, the temperature of column was 40°C with injection volume of 1 μL. TOF/MS and electrospray ion (ESI) source were applied for the qualitative analysis under the negative ion mode, and the full mass scan range was m/z 100-1500. Results: According to MS principle, twenty-five triterpenoids were identified from the methanol extract of Akebiae Fructus and the chemical structures of other nine unknown compounds were deduced. Conclusion: UFLC-Q-TOF/MS method could identify the main chemical constituents in Akebiae Fructus rapidly and accurately, which lays a foundation for the quality control of Akebiae Fructus.