Exposure of sexually inactive males to estrogenized females increased the investigative and consummatory sexual behavior.

Two trials were conducted to evaluate the effect of diverse socio-sexual cues upon male sexual behavior and the reproductive performance of anestrous does (AD). Trials were conducted in northern Mexico (26°N) during the natural anestrous season (Feb-Mar) with crossbred dairy bucks. In Experiment 1, sexually inactive bucks (SIB, n=12) were randomly allotted to three groups, four males/group: a) DEE 9novelty stimulation) - daily exchange of estrogenized females (EF) 12&12h, b) NEE (no novelty stimulation) - no-exchange of EF, 24h, or c) CON (saline-treated_ -daily exchange of AF 12&12h. Sexually active bucks (SAB) from the DEE, NEE and CON groups were subsequently exposed to AD (n=72; n=24/group) and the reproductive outcomes were recorded. In Experiment 2, SAB (n=12; n=6/group) were randomly divided in: 1) B+EF - males+four-EF exposed to AF (n=36), and 2) B+NEF; males+four-saline-treated AD and exposed to AD (n=36). Prior to the onset of the experimental breeding in both experiments, the investigative (ISB), consummatory (CSB) and resting (RSB) sexual behavior of males were quantified (2h×d×2d). Sexual behaviors considered were: ISB - flehmen, ano-genital sniffing, approaches, vocalizations, kicking, penis extrusion, CSB; mount attempts and mounts, and RSB - isolation, attempted escape, aggression and distractions. While EF were an effective stimulus (P<0.05) for evoking mounting in SIB males, daily exchange of estrous does used to stimulate males promoted an enhanced response (P<0.05) in terms of both ISB and CSB. After being exposed to AD, the B+EF bucks induced an earlier estrous response (P<0.05) as compared with the B+NEF bucks. The untreated females did not induce any sexual activity in males and stimulation of ovarian function did not occur when saline treated (CON) AD were exposed to AD. Also, the B+EF group induced an enhanced increase (P<0.05) of the male ISB and CSB, inducing in turn an increase percentage onset of estrus in does that had previously been anestrus (P<0.05).

Sacrocolpopexia laparoscópica para el tratamiento del prolapso apical: resultados y seguimiento a largo plazo / Laparoscopic sacrocolpopexy for apical prolapse

Objetivos: Evaluar los resultados subjetivos, anatómicos y funcionales a largo plazo de las pacientes sometidas a sacrocolpopexia laparoscópica para manejo de prolapso apical. Métodos: Estudio observacional con pacientes a quienes se les hizo sacrocolpopexia laparoscópica entre febrero de 2006 y diciembre de 2012, en tres centros. El nivel de soporte del piso pélvico se midió mediante la escala de cuantificación del prolapso de órganos pélvicos (POP-Q). Los resultados funcionales se evaluaron mediante un cuestionario de síntomas intestinales, urinarios, sexuales y de molestias físicas. También se estimó la satisfacción global de las pacientes con una escala de uno a diez. Resultados: Se realizó sacrocolpopexia laparoscópica a 68 pacientes, pero el seguimiento fue posible sólo en 24. Ninguna paciente tuvo prolapso apical postoperatorio. El punto C medio del POP-Q fue -6,8 cm. Se observó mejoría importante con respecto a los síntomas subjetivos de prolapso con reducciones significativas en las puntuaciones del cuestionario en el seguimiento postoperatorio. La satisfacción fue en promedio de 9,1. La incontinencia urinaria preoperatoria se resolvió en el 35 por ciento de las que la reportaron, sin necesidad de cirugía de continencia concomitante. No hubo complicaciones a corto plazo. A largo plazo hubo una hernia incisional en el sitio del trocar y una obstrucción intestinal por la malla. Conclusión: La sacrocolpopexia laparoscópica es un tratamiento quirúrgico seguro y eficaz para el prolapso apical post-histerectomía. Proporciona un excelente soporte apical y buen nivel de satisfacción, con una mejoría general de los síntomas de prolapso.

Objective: To evaluate the long-term subjective, anatomical and functional outcomes after laparoscopic sacrocolpopexy for apical prolapse. Methods: An observational study of women undergoing laparoscopic sacrocolpopexy between February 2006 and December 2012 was undertaken, at three centers. Pelvic organ support was assessed objectively using the pelvic organ prolapse quantification scale (POP-Q). Functional outcomes were assessed using a questionnaire of bowel, urinary, sexual and physical discomfort symptoms postoperatively. Also was assessed the overall satisfaction of surgery with a scale of one to ten. Results: During the period of the study, sacrocolpopexy was done in 68 patients, but follow-up was possible only in 24. At a mean follow up of 34 months, all 24 women had stage 0 vault support with point C of the POP-Q score averaging -6.8 cm. Subjective improvements in prolapse symptoms were observed with significant reductions in the questionnaire scores. The satisfaction measured with visual scale averaged 9.1. Fifteen women reported stress urinary incontinence before sacrocolpopexy, and it was resolved in 35 percent without concomitant continence surgery. New onset incontinence was reported in two women. There were no intraoperative and perioperative complications. The long term complications were an incisional hernia on the trocar port site and bowel obstruction caused by the mesh that needed intestinal resection. Conclusions: Laparoscopic sacrocolpopexy is a safe and effective surgical treatment for post-hysterectomy apical prolapse. It provides excellent apical support and good level of satisfaction, with overall improvement in prolapse symptoms.

Noise trauma impairs neurogenesis in the rat hippocampus.

The hippocampus, a major site of neurogenesis in the adult brain, plays an important role in memory. Based on earlier observations where exposure to high-intensity noise not only caused hearing loss but also impaired memory function, it is conceivably that noise exposure may suppress hippocampal neurogenesis. To evaluate this possibility, nine rats were unilaterally exposed for 2 h to a high-intensity, narrow band of noise centered at 12 kHz at 126 dB SPL. The rats were also screened for noise-induced tinnitus, a potential stressor which may suppress neurogenesis. Five rats developed persistent tinnitus-like behavior while the other four rats showed no signs of tinnitus. Age-matched sham controls showed no signs of hearing loss or tinnitus. The inner ear and hippocampus were evaluated for sensory hair cell loss and neurogenesis 10 weeks post-exposure. All noise exposed rats showed severe loss of sensory hair cells in the noise-exposed ear, but essentially no damage in the unexposed ear. Frontal sections from the hippocampus were immunolabeled for doublecortin to identify neuronal precursor cells, or Ki67 to label proliferating cells. Noise-exposed rats showed a significant reduction of neuronal precursors and fewer dividing cells as compared to sham controls. However, we could not detect any difference between rats with behavioral evidence of tinnitus versus rats without tinnitus. These results show for the first time that high intensity noise exposure not only damages the cochlea but also causes a significant and persistent decrease in hippocampal neurogenesis that may contribute to functional deficits in memory.

Molecular determinants for apical expression of the renal type IIa Na+/Pi-cotransporter.

Type IIa and IIb Na+/Pi-cotransporters have different patterns of expression in vivo: IIa is expressed in apical membranes of renal proximal tubules, and IIb in intestinal and lung epithelia. They are found in different subcellular locations when transfected in epithelial cells: IIa is apically expressed in renal proximal cells (OK), but mostly intracellularly in intestinal cells (CaCo2); IIb is apical in both cell types. To identify the domains responsible for the different expression of both cotransporters (in CaCo2), as well as those responsible for the apical expression of IIa (in OK), mutated cotransporters were fused to the Enhanced Green Fluorescent Protein (EGFP), and their expression analyzed by confocal microscopy. We conclude that the apical expression information for CaCo2 is contained within the C-terminal tail of IIb, but is not contained within IIa. From analysis of mutated IIa cotransporters we identified residues, within the C-terminal tail, involved in the apical expression of these cotransporters in OK cells: internal PR-residues and terminal TRL-residues. These signals are functional in OK but not in CaCo2-cells, supporting the concept that polarized targeting can be protein and cell specific.

Molecular determinants for apical expression and regulatory membrane retrieval of the type IIa Na/Pi cotransporter.

Renal inorganic phosphate (Pi) reabsorption is a key process in Pi homeostasis. Type IIa Na/Pi cotransporters, located at the apical membrane of renal proximal tubular cells, guarantee the vectorial transport of Pi. Renal Pi reabsorption can be modulated by controlling the number of cotransporters expressed at the apical membrane. Indeed, factors that increase Pi reabsorption induce the expression of type IIa cotransporters at the apical membrane, whereas factors that decrease Pi reabsorption lead to their retrieval. Therefore, proper sorting of this type of cotransporters is an essential step in Pi homeostasis. The relevance of polarization has been highlighted by the finding that improper sorting of transporters can cause disease. Here we describe the identification of signals involved in apical expression of newly synthesized type IIa cotransporters and in their hormonal-induced endocytosis.

Dopamine acutely stimulates Na+/H+ exchanger (NHE3) endocytosis via clathrin-coated vesicles: dependence on protein kinase A-mediated NHE3 phosphorylation.

Dopamine (DA) is a key hormone in mammalian sodium homeostasis. DA induces natriuresis via acute inhibition of the renal proximal tubule apical membrane Na(+)/H(+) exchanger NHE3. We examined the mechanism by which DA inhibits NHE3 in a renal cell line. DA acutely decreases surface NHE3 antigen in dose- and time-dependent fashion without altering total cellular NHE3. Although DA(1) receptor agonist alone decreases surface NHE3, simultaneous DA(2) agonist synergistically enhances the effect of DA(1). Decreased surface NHE3 antigen, caused by stimulation of NHE3 endocytosis, is dependent on intact functioning of the GTPase dynamin and involves increased binding of NHE3 to the adaptor protein AP2. DA-stimulated NHE3 endocytosis can be blocked by pharmacologic or genetic protein kinase A inhibition or by mutation of two protein kinase A target serines (Ser-560 and Ser-613) on NHE3. We conclude that one mechanism by which DA induces natriuresis is via protein kinase A-mediated phosphorylation of proximal tubule NHE3 leading to endocytosis of NHE3 via clathrin-coated vesicles.

A dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter.

Type II NaPi cotransporters are expressed in the apical membrane of P(i)-(re)absorbing epithelia: the type IIa in renal proximal tubule and the type IIb in small intestine. Parathyroid hormone (PTH) leads to a retrieval from the apical membrane of the type IIa NaPi cotransporter. The type IIa cotransporter is also expressed in opossum kidney (OK) cells, and its expression is under the control of PTH. In the present study, we identified the molecular "domains" involved in the PTH-induced retrieval of the type IIa NaPi cotransporter. Wild-type mouse type IIa (mIIa) and type IIb (mIIb) as well as several mIIa-mIIb chimeras and site-directed mutants were fused to the enhanced green fluorescent protein and transfected into OK cells. We found that mIIa but not mIIb was internalized and degraded after incubation with 1-34 (or 3-34) PTH. Using chimeras, we found that the N and C termini were not required in this effect, whereas a "domain" located between residues 216 and 658 seemed to be necessary. This region contains two putative intracellular loops with highly conserved sequences between mIIa and mIIb; in the last intracellular loop, two charged amino acids of type IIa (K(503)R(504)) are replaced by uncharged residues in type IIb (N(520)I(521)). We generated two mutants in which these residues were interchanged: mIIaNI and mIIbKR. Similarly to mIIa, the mIIbKR mutant was endocytosed in response to 1-34 PTH; in contrast, mIIaNI behaved as mIIb and was not internalized. In conclusion, a dibasic amino acid motif (K(503)R(504)) located in the last intracellular loop of the type IIa NaPi cotransporter is essential for its PTH-induced retrieval.

Effects of intravenous cocaine administration on cerebellar Purkinje cell activity.

The goal of the present study was to investigate the effects of intravenous cocaine administration on cerebellar Purkinje cell firing. Extracellular neuron activity was recorded and cells were locally excited with spaced microiontophoretic pulses of glutamate. Glutamate-evoked and spontaneous discharges were compared before and immediately following cocaine administration. Cocaine injections (1. 0 and 0.25 mg/kg, i.v.) induced a reversible suppression of both spontaneous activity and glutamate-evoked excitation. Procaine was ineffective in producing similar actions. Cocaine only inhibited glutamate-induced excitation in animals pre-treated with reserpine (5 mg/kg, i.p.). Propranolol injections (10 mg/kg, i.p.) were ineffective in blocking cocaine-induced inhibitions. Yohimbine (5 mg/kg, i.p.) pre-treatment abolished cocaine-induced suppressions of either spontaneous or glutamate-evoked excitation. Therefore, cocaine administration decreases Purkinje cell spontaneous and glutamate-evoked discharges by a mechanism involving alpha(2)-adrenoceptor activation. It is suggested that by changing the normal function of the cerebellum cocaine can produce drug-related alterations in overt behavior and cognition.

Requirement of a leucine residue for (apical) membrane expression of type IIb NaPi cotransporters.

Type II NaPi cotransporters mediate epithelial phosphate (P(i)) reabsorption. In mammals the type IIb protein is expressed in the small intestinal apical membrane and other epithelia; it is not expressed in the renal proximal tubule where we find the type IIa isoform. To look for molecular determinant(s) involved in apical expression of type IIb cotransporters, we have made deletion mutations within the C-terminal tails of mouse IIb (mIIb) and human IIb (hIIb) transporter proteins. The constructs were fused to the enhanced green fluorescent protein and transiently transfected into intestinal CaCo2-cells. Both mIIb and hIIb were located exclusively in the apical membrane of the cells. For mIIb, the removal of a cysteine cluster or the last three amino acids (TVF) had no effect on the location of the protein. However, truncation at the level of the conserved L691/689 prevented the apical membrane expression of both mIIb and hIIb, respectively, and the mutated proteins were located in endosomal and lysosomal structures. A similar expression pattern of the mIIb and hIIb constructs was found in renal proximal tubular opossum kidney cells. Our data suggest that L691/689 is involved in mechanisms leading to an apical expression of type IIb NaPi cotransporters.

Asymmetrical targeting of type II Na-P(i) cotransporters in renal and intestinal epithelial cell lines.

Targeting of newly synthesized transporters to either the apical or basolateral domains of polarized cells is crucial for the function of epithelia, such as in the renal proximal tubule or in the small intestine. Recently, different sodium-phosphate cotransporters have been identified. Type II cotransporters can be subdivided into two groups: type IIa and type IIb. Type IIa is predominantly expressed in renal proximal tubules, whereas type IIb is located on the intestinal and lung epithelia. To gain some insights into the polarized targeting of the type II cotransporters, we have transiently expressed type IIa and type IIb cotransporters in several epithelial cell lines: two lines derived from renal proximal cells (opossum kidney and LLC-PK(1)), one from renal distal cells (Madin-Darby canine kidney), and one from colonic epithelium (CaCo-2). We studied the expression of the transporters fused to the enhanced green fluorescent protein. Our data indicate that the polarized targeting is dependent on molecular determinants most probably located at the COOH terminus of the cotransporters as well as on the cellular context.

Estudio clínico de eficacia y tolerancia con sultamicilina fipexiam en procesos infecciosos de tratamiento ambulatorio / Clinic study of efficacy and toleration with sultamicilina fipeham in infectious process of ambulatory treatment

Se realizó un estudio abierto, multicéntrico, no comparativo donde se avaluó la eficacia y tolerancia de la combinación Ampicilina/Sulbatam por vía oral-Sultamicilina-(Fipexiam), en adultos y niños con infecciones del tracto respiratorio superior e inferior y con infeciones de piel y partes blandas, y adultos con Enfermedad Inflamatoria Pélvica (EIP). El estudio reunió los investigadores de 73 Centros. Se trataron un total de 195 pacientes obteniéndose una efectividad global de 93.3 por ciento. De estos pacientes 84 presentaron Otitis media aguda, resultando curas clínicas en el 89.2 por ciento, 67 presentaron procesos orofaríngeos con curación en el 94 por ciento; en los casos con infecciones de piel y partes blandas, así como en las mujeres tratadas con (EIP) la curación clínica fue de 100 por ciento. Se reportaron efectos adversos en 10.9 por ciento, siendo las molestias gastrointestinales más resaltantes con 8.7 por ciento de los casos reportados, siendo menores a lo reportado por la literatura médica

Dorso curvo / Curve dorse

El dorso curvo es una patología frecuente en la infancia y adolescencia, por lo tanto, el pediatra puede verse enfrentado en numerosas oportunidades con el problema. Tanto los padres como los médicos pueden notar la anormalidad y asumir que se trata sólo de un problema postural, restándole la importancia que se merece. La experiencia demuestra que frecuentemente lo que se encuentra bajo este aparente problema postural es una alteración estructural de la columna vertebral, cuyo reconocimiento y tratamientoprecoz permite llegar a resultados satisfactorios sobre la deformidad y sobre las consecuencias uque esta anomalía podría provocar de ser abandonada a su suerte

Estrabismo: aportes al pediatra general / Strabismus: contribution to the general pediatrist

El estrabismo es un motivo frecuente de inquietud y consulta por parte de los padres. Los pediatras requieren una adecuada evaluación del paciente para evitar secuelas que pueden ir desde un problema estético hasta ambliopía. Todo niño que manifieste una desviación de la mirada durante varias semanas o que aparezca después de los 6 meses de edad, debe ser derivado al oftalmólogo para determinar la causa del estrabismo. El pediatra debe además supervisar la evolución y el cumplimiento de las indicaciones del especialista

Effect of myotonia induced by anthracene-9-carboxylic acid on mitochondrial calcium, plasma creatinine-phosphokinase and aldolase activity in the rat.

The frequent association of myotonia with dystrophy and the knowledge that calcium is increased in injured skeletal muscle cells suggest a possible relationship between cell calcium and myotonic alterations. This investigation has been performed to study the role of calcium in experimental myotonia induced by anthracene-9-carboxylic acid (9-AC) in rats treated with several regimens of food and exercise. Thirty-two rats were divided into 4 groups of 8 rats each, one control and 3 experimental groups. The treatments included caffeine plus exercise (group 2), and a calcium-rich diet (group 3); these procedures were designed to increase intracellular calcium; another group was treated with 9-AC as a myotonia-inducer (group 4). The treatment for all groups lasted 60 days. No significant differences in plasma sodium, potassium, chloride and calcium between control and experimental groups were observed. Whole muscle calcium in wet tissue samples did no change with any treatment. On the contrary, mitochondrial calcium showed a significantly higher concentration in group 3 and 4. CPK and aldolase activities in groups 1, 2 and 3 were similar; but in group 4 these enzyme activities were significantly higher (p less than 0.05). The electrical and mechanical responses were not altered in any rat with any experimental treatment. Our data suggest that myotonia is a predisposing factor for an altered mitochondrial calcium homeostasis in this model; in addition, the enzyme activities of CPK and aldolase were increased in the rats of group 4 implicating that myotonia is a crucial factor in the development of enzymatic abnormalities.(ABSTRACT TRUNCATED AT 250 WORDS)

Detección laboratorial de ETS en la población encarcelada en la ciudad de Cochabamba 1991-1992 / Laboratory detection of sexual transmisible diseases in the people in the jail of Cochabamba

Desde la década del 70 la denominación de enfermedades veneras, ha sido reemplazada mundialmente por la de enfermedades de Transmisión Sexual (E.T.S.) concepto que permite mayor perspectiva del problema. Al hablar de ETS nos referimos a enfermedades infecciosas de diversas etiologías (bacterianas, virus, hongos etc) cuyo mecanismo de transmisión mas importante es el contacto sexual, generando este tipo de contagio una demanda de atención médica. De esta manera al antiguo término de venéreas que designaban a las enfermedades clásicas: Lues Gonorrea, Chancro blando, Granulona inguinal y Linfogranulona venéreo, se incorporan otras infecciones cuyo mecanismo de transmisión se ha identificado como sexual. Tal es el ejemplo del Herpes genital tipo II y el SIDA. Cada enfermo de ETS es el resultado de un contacto, constituyendose ademas en una fuente de infección, alargando de esta manera la cadena de epidemiológica. Por lo que es importante el diagnóstico oportuno para instituir un tratamiento adecuado y realizar control de contactos, hecho que nos ha motivado a realizar el presente trabajo. El mismo que consistió en la obtención de datos estadísticos laboratoriales del año 1991 donde la obtención de muestras fue del 100 de la población en estudio un tanto en la carcel de mujeres como en los de varones. Se escogió este universo de trabajo por ser una población de alto riesgo por la promiscuidad y el ascinamiento en el que viven. Las patologías a investigarse son: Gonorrea, Sifilis y SIDA por ser las entidades de notificación obligatoria. Se utilizó el método retrospectivo analítico comparativo no experimental: tomanado en cuenta las variables de: edad, sexo, estado civil, nivel socieconómico y cultural número de parejas sexuales proceden. Los datos personales laboratorialmente positivas se tomaron un protocolo exclusivo diseñado para el efecto, luego se realizo el respectivo procesamiento de datos y tratamiento estadístico, finalmente elaboración de resultados. Los mismos que son los siguientes: la edad mas afectada es de 20 a 29 años con un 46 con respecto al sexo es el masculino el que ocupa el mayor pocentaje con 71 por ciento el estado civil que ocupa el primer lugar en frecuencia es el casado con un 37 tomando en cuenta la procedencia el area urbana es mas afectada con 66 por ciento frente al area rural que tiene un porcentaje de 34. El nivel cultural que se presento mayor número de casos fue el intermedio con un 41, los mas bajos fueron el analfabeto...

Prevalencia de enfermedades articulares en la CNS de Cochabamba / Prevalence of joint diseases of the CNS of Cochabamba

El trabajo de investigación, consiste en una revisión por muestreo aleatorio (10 por ciento) de las carpetas familiares de los policonsultorios de Medicina Familiar de la C.N.S., en el Departamento de Cochabamba, eligidos a la zar de los policonsultorios: Central 32, Cala Cala, Villa Galindo, Quillacollo, Sacaba y Punata de las cuales se ha determinado la prevalencia de las enfermedades articulares de familias aseguradas que asistieron en forma regular a la consulta médica durante el periodo de 1980 a 1990. Durante el proceso de investigación se utilizo el método de la encuesta comparativa retrospectiva institucional no experimental: tomando en cuenta las variables: sexo, edad, ocupación, procedencia, diagnóstico, tratamiento, estado socioeconómico y nivel cultural. De acuerdo a los resultos finales, se ha demostrado la alta prevalencia de estas patologías en cada area, policonsultorio y grupo familiar asegurada a la C.N.S. de Cochabamba, de las 580 carpetas familiares revisadas se han registrado 1.338 historias clínicas y de las cuales 174 pacientes padecen de enfermedad articular, lo cual representa el 13 por ciento del total de revisados. Las patologías mas prevalentes, en forma decreciente, son la Artritis Reumatoide con 51 por ciento, Osteoartritis con 25 por ciento, gota con el 15 por ciento y la Fiebre Reumática con 9 por ciento del total de afecciones. Ademas se aha averificado alta prevalencia de estas patologías en sexo femenino, en grupo etareo de 30 a 60 años de edad y en pacientes de ocupación en labores de casa, rentistas y del magisterio.

Efecto de la intervención nutricional en pacientes con hiperlipoproteinemia tipo V / Effect of the nutricional intervention in patients with hyperlipoproteinemia type V

La hiperlipoproteinemia tipo V, es una alteración en el metabolismo de las lipoproteínas, que se caracteriza por hipertrigliceridemia servera. El presente estudio muestra el efecto de una intervención nutricional sobre las concentraciones séricas de triglicéridos y colesterol en trece pacientes...