Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill.

The environmental and economic impact of an oil spill can be significant. Biotechnologies applied during a marine oil spill involve bioaugmentation with immobilised or encapsulated indigenous hydrocarbonoclastic species selected under laboratory conditions to improve degradation rates. The environmental factors that act as stressors and impact the effectiveness of hydrocarbon removal are one of the challenges associated with these applications. Understanding how native microbes react to environmental stresses is necessary for effective bioaugmentation. Herein, Micrococcus luteus and M. yunnanensis isolated from a marine oil spill mooring system showed hydrocarbonoclastic activity on Maya crude oil in a short time by means of total petroleum hydrocarbons (TPH) at 144 h: M. luteus up to 98.79 % and M. yunnanensis 97.77 % removal. The assessment of Micrococcus biofilms at different temperature (30 °C and 50 °C), pH (5, 6, 7, 8, 9), salinity (30, 50, 60, 70, 80 g/L), and crude oil concentration (1, 5, 15, 25, 35 %) showed different response to the stressors depending on the strain. According to response surface analysis, the main effect was temperature > salinity > hydrocarbon concentration. The hydrocarbonoclastic biofilm architecture was characterised using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Subtle but significant differences were observed: pili in M. luteus by SEM and the topographical differences measured by AFM Power Spectral Density (PSD) analysis, roughness was higher in M. luteus than in M. yunnanensis. In all three domains of life, the Universal Stress Protein (Usp) is crucial for stress adaptation. Herein, the uspA gene expression was analysed in Micrococcus biofilm under environmental stressors. The uspA expression increased up to 2.5-fold in M. luteus biofilms at 30 °C, and 1.3-fold at 50 °C. The highest uspA expression was recorded in M. yunnanensis biofilms at 50 °C with 2.5 and 3-fold with salinities of 50, 60, and 80 g/L at hydrocarbon concentrations of 15, 25, and 35 %. M. yunnanensis biofilms showed greater resilience than M. luteus biofilms when exposed to harsh environmental stressors. M. yunnanensis biofilms were thicker than M. luteus biofilms. Both biofilm responses to environmental stressors through uspA gene expression were consistent with the behaviours observed in the response surface analyses. The uspA gene is a suitable biomarker for assessing environmental stressors of potential microorganisms for bioremediation of marine oil spills and for biosensing the ecophysiological status of native microbiota in a marine petroleum environment.

Achievements and challenges in the health of Mexican women, 1990 and 2019: ecological analysis based on the global burden of disease data.

OBJECTIVES: To describe the burden and causes of disease in Mexican women in 1990 and 2019, based on the data disaggregation by age groups and states. Also, to evaluate the relationship of years of healthy life lost with the Socio-demographic Index (SDI) and with the Healthcare Access and Quality (HAQ) Index. STUDY DESIGN: This was an ecological descriptive study. METHODS: Based on the Global Burden of Disease, Injuries, and Risk Factors Study study, the age-standardized and age-specific rates for mortality, years of life lost (YLLs), years lived with disability (YLDs), and disability-adjusted life years (DALYs) were reported. RESULTS: At the national level, the all-cause age-standardized rates for Mexican women decreased in mortality -28.8%; YLLs -39.8%; YLDs -1.3%; and DALYs -26.2%. For 2019, the indicators analyzed had the worst performances in Chiapas and Chihuahua, while women in Sinaloa had the lowest age-standardized rates. In 1990, it is worth noting that there was a remarkable presence of CDs, mainly in YLLs. In all age groups, diabetes mellitus was the leading cause of DALYs in Mexico's 32 states, followed by CKD (in 24 states), and ischemic heart disease (in 18 states). In both 1990 and 2019, a negative and statistically significant correlation between DALYs and the HAQ Index was evident. The correlation between DALYs and the SDI was only significant in 1990. CONCLUSION: In the last 30 years, the burden of disease on Mexican women has undergone substantial changes that reflect progress in the improvement of their health conditions. However, the current scenario is complex because the convergence of communicable diseases, non-communicable diseases, and injuries is evident, which implies important challenges that must be addressed as soon as possible.

A guide to classify tattoo motives in Mexico as a tool to identify unknown bodies.

JUSTIFICATION: In Mexico, the number of unidentified bodies has been steadily rising for years. By now, more than 50,000 bodies are considered unidentified. Forensic laboratories that could perform comparative molecular genetic investigation are often overburdened and examinations can take months. Therefore, pragmatic approaches that can help to identify more unknown bodies must be sought. The increased use of distinctive physical features might be one, and the high rate of tattooed people in Mexico points towards a great potential of tattoos as a tool for identification. The prerequisite for a comparison of antemortem (missing persons) and postmortem (unknown bodies) data is an objective description of the particularities, e.g., of the tattoos. The aim of this study was to establish an objective classification for tattoo motives, taking into consideration local preferences. METHODS: In the database of the medicolegal services of the Instituto Jaliscience de Ciencias Forenses (IJCF) in Guadalajara, postmortem data of 1000 tattooed bodies from 2019 were evaluated. According to sex and age, the tattooed body localization and the tattoo motives were categorized. RESULTS: The 1000 tattooed deceased showed tattoos on 2342 body localizations. The motives were grouped and linked to the following 11 keywords (with decreasing frequency): letters/numbers, human, symbol (other), plant, symbol (religious), animal, object, fantasy/demon/comic, tribal/ornament/geometry, other, unrecognizable. CONCLUSION: Using the proposed classification, tattoo motives can be described objectively and classified in a practical way. If used for antemortem (missing persons) and postmortem (unknown bodies) documentation, motives can be searched and compared efficiently-helping to identify unknown bodies.

[Coraco-clavicular loop and tension band suture in type II and type V distal-third clavicle fractures]. / Cerclaje coracoclavicular y banda de tensión con sutura en el tratamiento de fracturas del tercio externo de la clavícula tipos II y V.

Many surgical techniques have been used to address unstable distal third clavicle fractures. Complications and the need for hardware removal are still a concern. We propose a surgical technical using high-strength sutures to restore vertical and horizontal stability in Neer type II and Neer type V distal-third clavicle fractures. It has been used in three cases; two type V and one type II. In all cases, bone healing was achieved uneventfully and all patients resumed their pre-injury activities including sports at sixmonths postoperatively. The coraco-clavicular loop and tension band suture technique is a simple procedure that allows vertical and horizontal stabilization of the fracture. It achieves good clinical results and it may be a costeffective alternative to other techniques although a longer series and longterm followup is required to adequately assess the results.

Se han descrito diferentes técnicas quirúrgicas para el tratamiento de las fracturas inestables del tercio distal de la clavícula. Las complicaciones asociadas y la necesidad de retirar los implantes siguen siendo motivo de preocupación. Proponemos una técnica quirúrgica que utiliza suturas de alta resistencia para restaurar la estabilidad vertical y horizontal en las fracturas del tercio distal de la clavícula de tipo II y V de la clasificación de Neer. Esta técnica se ha utilizado en tres casos; dos de tipo V y uno de tipo II. En todos se obtuvo la consolidación ósea y todos reanudaron sus actividades previas a la lesión, incluyendo actividad deportiva, a los seis meses de la cirugía. La técnica de cerclaje coracoclavicular y banda de tensión con sutura es un procedimiento sencillo que permite la estabilización vertical y horizontal de la fractura. Permite obtener buenos resultados clínicos y puede ser una alternativa coste-efectiva eficaz en el tratamiento de estas lesiones aunque se requiere una serie más larga y un seguimiento a largo plazo para evaluar adecuadamente los resultados.

Cerclaje coracoclavicular y banda de tensión con sutura en el tratamiento de fracturas del tercio externo de la clavícula tipos II y V / Coraco-clavicular loop and tension band suture in type II and type V distal-third clavicle fractures

Resumen: Se han descrito diferentes técnicas quirúrgicas para el tratamiento de las fracturas inestables del tercio distal de la clavícula. Las complicaciones asociadas y la necesidad de retirar los implantes siguen siendo motivo de preocupación. Proponemos una técnica quirúrgica que utiliza suturas de alta resistencia para restaurar la estabilidad vertical y horizontal en las fracturas del tercio distal de la clavícula de tipo II y V de la clasificación de Neer. Esta técnica se ha utilizado en tres casos; dos de tipo V y uno de tipo II. En todos se obtuvo la consolidación ósea y todos reanudaron sus actividades previas a la lesión, incluyendo actividad deportiva, a los seis meses de la cirugía. La técnica de cerclaje coracoclavicular y banda de tensión con sutura es un procedimiento sencillo que permite la estabilización vertical y horizontal de la fractura. Permite obtener buenos resultados clínicos y puede ser una alternativa coste-efectiva eficaz en el tratamiento de estas lesiones aunque se requiere una serie más larga y un seguimiento a largo plazo para evaluar adecuadamente los resultados.

Abstract: Many surgical techniques have been used to address unstable distal third clavicle fractures. Complications and the need for hardware removal are still a concern. We propose a surgical technical using high-strength sutures to restore vertical and horizontal stability in Neer type II and Neer type V distal-third clavicle fractures. It has been used in three cases; two type V and one type II. In all cases, bone healing was achieved uneventfully and all patients resumed their pre-injury activities including sports at six-months postoperatively. The coraco-clavicular loop and tension band suture technique is a simple procedure that allows vertical and horizontal stabilization of the fracture. It achieves good clinical results and it may be a cost-effective alternative to other techniques although a longer series and long-term follow-up is required to adequately assess the results.

Análisis y autoevaluación de las competencias tutoriales en un programa de posgrado en enfermería / Analysis and self-assessment of the tutorial competences in a nursing graduate program / Análise e autoavaliação das competências tutoriais em um programa de pós-graduação em enfermagem

RESUMEN Introducción: El tutor es fundamental en la formación de estudiantes del posgrado, su trabajo no se enfoca únicamente en la dirección de la tesis; además debe fomentar el desarrollo de otras habilidades en el alumno al incorporarlo al conocimiento tácito en comunidades de investigación. Esto implica que el tutor muestre competencias en la tutoría, de ahí la importancia de reconocerlas y autoevaluarlas tanto en forma crítica como reflexiva para su mejora. Objetivo: Analizar y autoevaluar las competencias de los tutores de un posgrado en enfermería. Método: Los tutores fueron invitados a participar de manera libre y voluntaria en grupos focales, con el propósito de analizar, discutir y autoevaluar sus competencias; se empleó una rúbrica como elemento detonador, que describe cualitativamente diez competencias que deben tener los tutores de posgrado, en cuatro niveles de dominio. Resultados: La discusión sostenida al interior de los grupos focales fue documentada para su codificación y análisis, se obtuvieron categorías que aludieron a: 1) aspectos éticos y el respeto a las autorías de los estudiantes en publicaciones; 2) la relevancia de la transferencia del conocimiento a problemas específicos del campo de enfermería; 3) la relación tutor-alumno destacando vínculos de menor y mayor dependencia. Conclusiones: Se identificó el reconocimiento de las competencias que requieren los tutores en este nivel, se generó una autoevaluación crítica y reflexiva, sobre lo que se ha realizado en la práctica educativa. Los tutores ubicaron sus niveles de competencia en desarrollo y consumado.

ABSTRACT Introduction: Tutors are fundamental in the formation of graduate students and their work is not only focused on the direction of the students' theses. Tutors also encourage the development of other skills, including the integration of students into research communities. This implies that tutors must demonstrate tutoring competencies; and therefore, it is important to recognize and assess these competencies from critical and reflexive points of view. Objective: To analyze and self-assess the competencies of tutors in a nursing graduate program. Method: Tutors were invited to participate in a free and voluntary manner in diverse focal groups with the aim of analyzing, discussing, and self-assessing their own competencies. Ten competencies in four domain levels were qualitatively assessed. Results: The discussion within the focal groups were documented and coded, and the following categories were identified: 1) ethical aspects and the respect of the authorship of students in publications; 2) the relevance of the transference of knowledge to specific problems in the field of nursing; 3) the tutor-student relationship highlighting the different levels of dependence associations. Conclusions: The identification of the competencies which tutors need to demonstrate at this curricular level was acknowledged. A critical and reflexive self-evaluation regarding the education practice was generated. Tutors identified their levels of competency.

RESUMO Introdução: O tutor é fundamental na formação de estudantes de pós-graduação, seu trabalho não se concentra unicamente na direção da tese; aliás deve fomentar o desenvolvimento de outras habilidades no aluno incorporando-o ao conhecimento tácito em comunidades de pesquisa. Isso implica que o tutor mostre competências na tutoria, daí a importância de reconhecê-las e auto avaliá-las de forma crítica e reflexiva para seu aprimoramento. Objetivo: Analisar e autoavaliar as competências dos tutores de uma pós-graduação em enfermagem. Método: Os tutores foram convidados para participar de maneira livre e voluntária em grupos focais, com o propósito de analisar, discutir e autoavaliar suas competências; foi utilizada uma rubrica como elemento desencadeador, que descreve qualitativamente dez competências que os tutores de pós-graduação devem ter, em quatro níveis de domínio. Resultados: A discussão realizada no interior dos grupos focais foi documentada para sua codificação e análise, obtiveram-se categorias que aludiram a: 1) aspectos éticos ao respeito da autoria dos estudantes em publicações; 2) a relevância da transferência do conhecimento para problemas específicos do campo de enfermagem; 3) a relação tutor-aluno evidenciando vínculos de menor e maior dependência. Conclusões: Identificou-se o reconhecimento das competências que os tutores requerem neste nível, gerou-se uma autoavaliação crítica e reflexiva, sobre o que tem sido realizado na prática educativa. Os tutores localizaram seus níveis de competência em desenvolvimento e alcançado.

Oviductal Proteins Effect in Rooster Spermatic Cryopreservation.

BACKGROUND: Cryopreservation induces spermatic cryo capacitation, which can decrease thawed sperm fertilizing capability. OBJECTIVE: To evaluate the effect of uterus-vaginal union protein factors to inhibit sperm cryo capacitation and maintain viability and fertilizing capability of rooster spermatozoa. MATERIALS AND METHODS: Rooster spermatozoa was cryopreserved using Lake extender supplemented with different hen's uterus-vaginal junction protein concentrations, to determine spermatic viability, sperm physiological condition and fertilizing capability in vivo. RESULTS: It was possible to induce spermatic decapacitation in vitro, inhibiting cryo capacitation and allowing fertility results comparable to those obtained with fresh semen. CONCLUSION: Uterus-vaginal protein extracts induce spermatic decapacitation in vitro.

Factores asociados a violencia horizontal intrahospitalaria en el personal de enfermería: revisión sistemática / Factors associated to intra-hospital horizontal violence against nursing personnel: a systematic review / Fatores associados a violência horizontal intrahospitalar no pessoal de enfermagem: revisão sistemática

Objetivo: Determinar factores de riesgo asociados a violencia horizontal intrahospitalaria en el personal de enfermería. Metodología: Revisión sistemática sobre estudios epidemiológicos que analizaron violencia horizontal y sus factores asociados. Se consultó Pubmed, ProQuest, ScienceDirect, Ebsco-Host, CINHAL, ISI Web of Science y Biblioteca Virtual en Salud. Se aplicó lectura crítica (STROBE), se otorgó nivel de evidencia y grado de recomendación con AHQR. Resultados: De 629 registros, 7 artículos fueron incluidos en la revisión sistemática. Todos obtuvieron nivel de evidencia III y grado de recomendación B. La prevalencia de violencia horizontal osciló entre 21.1-79.4%. Factores asociados a violencia horizontal fueron: lugar de trabajo, satisfacción con el trabajo, relaciones entre pares, plan para abandonar el trabajo y presencia de políticas relativas al acoso laboral. Conclusiones: Existe escasa evidencia del tema y de calidad moderada. Se hace necesario reconocer prevalencia de violencia horizontal y sus factores asociados, para diseñar estrategias de prevención en cultura laboral.

Objective: To determine risk factors associated to intra-hospital horizontal violence against nursing personnel. Methodology: This is a systematic review about epidemiological studies analyzing horizontal violence against nurses and its associated factors. Pubmed, ProQuest, ScienceDirect, Ebsco-Host, CINHAL, ISI Web of Science and Biblioteca Virtual en Salud databases were consulted. A critical lecture strategy (STROBE) was followed. AHQR-based Level of Evidence and Recommendation Degree were assigned. Results: From 629 registers, 7 articles were included in the systematic review. All received a Level of Evidence III and a Degree of Recommendation B. The prevalence of horizontal violence was found to be in the range of 21.1% to 79.4%. Associated risk factors were: place of work, job satisfaction, relationship with colleagues, intention to leave, and work harassment related policies. Conclusions: There is scant and limited in quality evidence on the topic. It becomes necessary to acknowledge the prevalence of intra-hospital horizontal violence against nurses and its associated factors in order to design prevention strategies which can strengthen the labor culture.

Objetivo: Determinar fatores de risco associados a violência horizontal intrahospitalar no pessoal de enfermagem. Metodologia: Revisão sistemática sobre estudos epidemiológicos que analisaram violência horizontal e seus fatores associados. Consultou-se Pubmed, ProQuest, ScienceDirect, Ebsco-Host, CINHAL, ISI Web of Science e Biblioteca Virtual en Salud. Aplicou-se leitura crítica (STROBE), outorgou-se nível de evidência e grau de recomendação com AHQR. Resultados: De 629 registros, 7 artigos foram incluídos na revisão sistemática. Todos obtiveram nível de evidência III e grau de recomendação B. A prevalência de violência horizontal oscilou entre 21.1-79.4%. Fatores associados a violência horizontal foram: lugar de trabalho, satisfação com o trabalho, relações entre pares, plano para abandonar o trabalho e presência de políticas relativas ao assédio laboral. Conclusões: Existe escassa evidência do tema e de qualidade moderada. É necessário reconhecer prevalência de violência horizontal e seus fatores associados, para desenhar estratégias de prevenção em cultura laboral.

Prótesis de recubrimiento en húmero proximal: ¿Cuál es la indicación hoy en día? / Proximal humeral resurfacing. What's today indication?

Resumen: Introducción: Los resultados funcionales de las prótesis de superficie en el húmero proximal son aceptables, pero con grandes diferencias en la tasa y causa de revisión según las diferentes series. El objetivo de nuestro trabajo fue analizar los resultados clínicos obtenidos en nuestro centro y tratar de definir al paciente ideal para este implante. Material y métodos: Estudio retrospectivo de 19 casos. Seguimiento de 31 (12-61) meses. La cirugía se indicó en casos de artrosis primaria o secundaria. Se analizaron datos demográficos, escala de Constant normalizada, cuestionario DASH, complicaciones y satisfacción. Hubo tres pérdidas durante el seguimiento por fallecimiento sin relación con la cirugía. Resultados: Edad media de 56 (25-80) años. La puntuación en la escala de Constant normalizada fue 73 (23-104) puntos y en el cuestionario DASH 31 (7-84) puntos. Noventa y cuatro por ciento de los pacientes retomaron sus actividades de ocio. En siete casos se objetivó radiológicamente colocación en varo del implante generando un conflicto inferior en la glena. Se produjo una capsulitis adhesiva resuelta de forma conservadora, tres reintervenciones por usura glenoidea sintomática y una por dolor incontrolable. Los pacientes sin lesión del manguito rotador presentaron mejor puntuación en los cuestionarios funcionales. Todos los pacientes refirieron estar satisfechos con la mejoría sintomática respecto a la situación previa. Conclusiones: Los resultados funcionales obtenidos son similares a los publicados previamente. Creemos que la prótesis de superficie debe ser considerada una opción en los casos de artrosis primaria o secundaria sin lesión del manguito rotador, independientemente de la edad.

Abstract: Introduction: Data published in the literature about humeral resurfacing prostheses are not conclusive with good functional results but with large differences in the revision rate. The aim of our study was to evaluate the clinical and functional outcomes in patients operated at our center. Material and methods: Retrospective study of 19 cases in 18 patients. Follow-up of 31 (12-61) months. Surgery was indicated in cases of primary or secondary osteoarthritis. Demographic data, Constant scale, DASH questionnaire, complications and satisfaction were analyzed. Three patients were lost to follow-up due to death unrelated to surgery. Results: Mean age of 56 (25-80) years. Constant normalized of 73 (23-104) points. DASH questionnaire of 31 (7-84) points. Ninety four percent of the patients resumed their recreational activities and 81% sports activities. In seven cases, inferior conflict in the glenoid with varus implant was observed radiologically. There were five complications; a conservatively resolved capsulitis, three reinterventions due to symptomatic inferior glenoid usury and another to uncontrollable pain in a conservative way. Patients without rotator cuff lesion presented better scores on the functional questionnaires. All patients were satisfied with the symptomatic improvement over the previous situation and would be operated again if necessary. Conclusions: The functional outcomes reported are similar to those previously published and, based on them, we believe that the resurfacing prosthesis is an option to be considered in cases of primary or secondary osteoarthritis when there is no rotator cuff injury, regardless of age.

[Subacromial sodium hyaluronate injection for the treatment of chronic shoulder pain: A prospective series of eighty patients]. / Infiltración subacromial de ácido hialurónico en el tratamiento del dolor crónico de hombro. Serie prospectiva de 80 pacientes seguidos durante un año.

OBJECTIVE: Our purpose was to assess the effectiveness of hyaluronic acid infiltrations for chronic shoulder pain as an alternative to the non-surgical and surgical treatments that are currently available. MATERIAL AND METHODS: This is a prospective study of 80 consecutive patients suffering from chronic shoulder pain followed for twelve months. Five subacromial hyaluronic acid injections on five consecutive weeks were administrated to all patients. RESULTS: A significant improvement within the whole group after six months was observed. Constant score improved by 7.7 points, DASH questionnaire decreased by 5 points and Visual Analog Scale for pain decreased by 1.6 points. Patients with history of less than 24 months of pain responded better to treatment. Females responded better. Patients with decreased subacromial space or cuff tear in the MRI improved but patients diagnosed by the MRI of acromioclavicular osteoarthritis worsened in all scales assessed. DISCUSSION: Subacromial hyaluronic acid injections are specially effective in patients with history of less than 24 months of pain, a decreased subacromial space or partial or total cuff tear but, in our experience, its result is not good in patients with acromioclavicular osteoarthritis.

OBJETIVO: Nuestro objetivo fue evaluar la efectividad de las infiltraciones subacromiales de ácido hialurónico en el tratamiento del dolor crónico de hombro como alternativa a los tratamientos quirúrgicos y no quirúrgicos actualmente disponibles. MATERIAL Y MÉTODOS: Estudio prospectivo de 80 pacientes consecutivos con dolor crónico de hombro seguidos durante 12 meses. Se realizaron cinco infiltraciones subacromiales con ácido hialurónico durante cinco semanas consecutivas y se analizaron los resultados clínicos obtenidos. RESULTADOS: La puntuación en la escala de Constant mejoró en 7.7 puntos, el cuestionario DASH disminuyó en cinco puntos y la puntuación en la escala visual analógica para el dolor disminuyó 1.6 puntos por término medio. Los pacientes que presentaban dolor de menos de 24 meses de evolución respondieron mejor al tratamiento. Las mujeres respondieron mejor. Los pacientes con disminución del espacio subacromial o lesión del manguito rotador según la resonancia magnética mejoraron, pero los pacientes que mostraron signos de artrosis acromioclavicular en la resonancia empeoraron en todas las escalas evaluadas. DISCUSIÓN: Las infiltraciones subacromiales de ácido hialurónico son especialmente efectivas en el tratamiento del dolor crónico de hombro de menos de 24 meses de evolución, con disminución del espacio subacromial o lesión parcial o total del manguito rotador, pero en nuestra experiencia, su resultado no es bueno en pacientes con artrosis acromioclavicular.

Infiltración subacromial de ácido hialurónico en el tratamiento del dolor crónico de hombro. Serie prospectiva de 80 pacientes seguidos durante un año / Subacromial sodium hyaluronate injection for the treatment of chronic shoulder pain: A prospective series of eighty patients

Resumen: Objetivo: Nuestro objetivo fue evaluar la efectividad de las infiltraciones subacromiales de ácido hialurónico en el tratamiento del dolor crónico de hombro como alternativa a los tratamientos quirúrgicos y no quirúrgicos actualmente disponibles. Material y métodos: Estudio prospectivo de 80 pacientes consecutivos con dolor crónico de hombro seguidos durante 12 meses. Se realizaron cinco infiltraciones subacromiales con ácido hialurónico durante cinco semanas consecutivas y se analizaron los resultados clínicos obtenidos. Resultados: La puntuación en la escala de Constant mejoró en 7.7 puntos, el cuestionario DASH disminuyó en cinco puntos y la puntuación en la escala visual analógica para el dolor disminuyó 1.6 puntos por término medio. Los pacientes que presentaban dolor de menos de 24 meses de evolución respondieron mejor al tratamiento. Las mujeres respondieron mejor. Los pacientes con disminución del espacio subacromial o lesión del manguito rotador según la resonancia magnética mejoraron, pero los pacientes que mostraron signos de artrosis acromioclavicular en la resonancia empeoraron en todas las escalas evaluadas. Discusión: Las infiltraciones subacromiales de ácido hialurónico son especialmente efectivas en el tratamiento del dolor crónico de hombro de menos de 24 meses de evolución, con disminución del espacio subacromial o lesión parcial o total del manguito rotador, pero en nuestra experiencia, su resultado no es bueno en pacientes con artrosis acromioclavicular.

Abstract: Objective: Our purpose was to assess the effectiveness of hyaluronic acid infiltrations for chronic shoulder pain as an alternative to the non-surgical and surgical treatments that are currently available. Material and methods: This is a prospective study of 80 consecutive patients suffering from chronic shoulder pain followed for twelve months. Five subacromial hyaluronic acid injections on five consecutive weeks were administrated to all patients. Results: A significant improvement within the whole group after six months was observed. Constant score improved by 7.7 points, DASH questionnaire decreased by 5 points and Visual Analog Scale for pain decreased by 1.6 points. Patients with history of less than 24 months of pain responded better to treatment. Females responded better. Patients with decreased subacromial space or cuff tear in the MRI improved but patients diagnosed by the MRI of acromioclavicular osteoarthritis worsened in all scales assessed. Discussion: Subacromial hyaluronic acid injections are specially effective in patients with history of less than 24 months of pain, a decreased subacromial space or partial or total cuff tear but, in our experience, its result is not good in patients with acromioclavicular osteoarthritis.

[Proximal humeral resurfacing. Whats today indication?] / Prótesis de recubrimiento en húmero proximal: ¿Cuál es la indicación hoy en día?

INTRODUCTION: Data published in the literature about humeral resurfacing prostheses are not conclusive with good functional results but with large differences in the revision rate. The aim of our study was to evaluate the clinical and functional outcomes in patients operated at our center. MATERIAL AND METHODS: Retrospective study of 19 cases in 18 patients. Follow-up of 31 (12-61) months. Surgery was indicated in cases of primary or secondary osteoarthritis. Demographic data, Constant scale, DASH questionnaire, complications and satisfaction were analyzed. Three patients were lost to follow-up due to death unrelated to surgery. RESULTS: Mean age of 56 (25-80) years. Constant normalized of 73 (23-104) points. DASH questionnaire of 31 (7-84) points. Ninety four percent of the patients resumed their recreational activities and 81% sports activities. In seven cases, inferior conflict in the glenoid with varus implant was observed radiologically. There were five complications; a conservatively resolved capsulitis, three reinterventions due to symptomatic inferior glenoid usury and another to uncontrollable pain in a conservative way. Patients without rotator cuff lesion presented better scores on the functional questionnaires. All patients were satisfied with the symptomatic improvement over the previous situation and would be operated again if necessary. CONCLUSIONS: The functional outcomes reported are similar to those previously published and, based on them, we believe that the resurfacing prosthesis is an option to be considered in cases of primary or secondary osteoarthritis when there is no rotator cuff injury, regardless of age.

INTRODUCCIÓN: Los resultados funcionales de las prótesis de superficie en el húmero proximal son aceptables, pero con grandes diferencias en la tasa y causa de revisión según las diferentes series. El objetivo de nuestro trabajo fue analizar los resultados clínicos obtenidos en nuestro centro y tratar de definir al paciente ideal para este implante. MATERIAL Y MÉTODOS: Estudio retrospectivo de 19 casos. Seguimiento de 31 (12-61) meses. La cirugía se indicó en casos de artrosis primaria o secundaria. Se analizaron datos demográficos, escala de Constant normalizada, cuestionario DASH, complicaciones y satisfacción. Hubo tres pérdidas durante el seguimiento por fallecimiento sin relación con la cirugía. RESULTADOS: Edad media de 56 (25-80) años. La puntuación en la escala de Constant normalizada fue 73 (23-104) puntos y en el cuestionario DASH 31 (7-84) puntos. Noventa y cuatro por ciento de los pacientes retomaron sus actividades de ocio. En siete casos se objetivó radiológicamente colocación en varo del implante generando un conflicto inferior en la glena. Se produjo una capsulitis adhesiva resuelta de forma conservadora, tres reintervenciones por usura glenoidea sintomática y una por dolor incontrolable. Los pacientes sin lesión del manguito rotador presentaron mejor puntuación en los cuestionarios funcionales. Todos los pacientes refirieron estar satisfechos con la mejoría sintomática respecto a la situación previa. CONCLUSIONES: Los resultados funcionales obtenidos son similares a los publicados previamente. Creemos que la prótesis de superficie debe ser considerada una opción en los casos de artrosis primaria o secundaria sin lesión del manguito rotador, independientemente de la edad.

Teratoma ovárico maduro e inmaduro, a propósito de un caso / About a case of mature and immature ovarian teratoma

Resumen: CASO CLÍNICO: paciente de 30 años de edad, con diagnóstico de teratoma inmaduro, con deseos de preservar la fertilidad. Se indicó tratamiento quirúrgico conservador y quimioterapia coadyuvante, previa vitrificación de ovocitos. Un año después de finalizar la quimioterapia logró embarazarse mediante fecundación in vitro, realizada con sus propios óvulos desvitrificados. Después de tres años de la intervención quirúrgica se detectó otro quiste en el ovario contralateral, que se intervino y diagnosticó como teratoma maduro. CONCLUSIONES: el teratoma ovárico inmaduro es una neoplasia poco frecuente cuyo tratamiento aún se discute. Puesto que la mayoría de las pacientes son jóvenes debe intentarse la preservación de la fertilidad proponiéndoles la preservación de ovocitos. Debido a la alta tasa de recurrencia del tumor, casi siempre en forma de teratoma maduro, es importante el seguimiento estrecho después de finalizar el tratamiento.

Abstract: CLINICAL CASE: We present a peculiar case in which an immature teratoma is diagnosed by an ovarian torsion in a 30 year old patient. She wanted to preserve her fertility, so she underwent conservative surgical treatment, previous vitrification of her oocytes. One year after the end of the chemotherapeutic treatment, the patient became pregnant through in vitro fertilization performed with her own devitrified oocytes. Another cyst in the contralateral ovary was diagnosed three years after the surgical intervention so she was reintervenated, it was a mature teratoma. CONCLUSIONS: Immature ovarian teratoma is an uncommon pathology whose treatment is controversial. Since most patients are young, we should try to preserve fertility if the patient wishes, by offering cryopreservation of oocytes when indicated. Due to the high rate of recurrence, often in the form of mature teratoma, it is important to follow-up closely after the treatment.

Técnica de Bristow-Latarjet en la inestabilidad glenohumeral anterior / Bristow-Latarjet Technique for anterior glenohumeral instability

Resumen: Antecedentes: En el tratamiento de la inestabilidad glenohumeral anterior se han descrito multitud de técnicas quirúrgicas, todas ellas con ventajas e inconvenientes. Nuestro objetivo es analizar los resultados obtenidos a medio plazo en los pacientes que hemos intervenido mediante la técnica de Bristow-Latarjet teniendo en cuenta el valor del ISIS preoperatorio. Material y métodos: Estudio retrospectivo de 33 pacientes intervenidos entre 2005 y 2012 con edad media de 33.2 años y seguimiento medio de 75 meses. Se recogió el resultado con las escalas de Rowe y Constant, cuestionario DASH y una encuesta de valoración subjetiva del resultado por parte de cada paciente. Resultados: No se presentaron recidivas. La puntuación media postoperatoria en la escala de Rowe fue de 74.6 puntos y de 70 puntos en la escala de Constant. En el cuestionario DASH el valor promedio fue 22.9 puntos. En 78.8% de los pacientes se valoró positivamente el resultado. Se produjo la migración del tornillo en un paciente, encontrándose asintomático. No se identificaron otras complicaciones. Conclusiones: La técnica de Bristow-Latarjet en el tratamiento de la inestabilidad glenohumeral anterior es una técnica fiable y con una mínima tasa de recidivas, por lo que debe emplearse como cirugía de elección en determinados casos y, para ello, la valoración preoperatoria con la escala ISIS es una buena guía a la hora de indicar el tipo de cirugía.

Abstract: Background: In treatment of anterior shoulder instability, many surgical techniques have been described, all of them with advantages and disadvantages. Our goal is to study the half term results on patients that underwent open Bristow-Latarjet surgery considering the preoperative ISIS value. Material and methods: This is a retrospective study of 33 patients which underwent open Bristow-Latarjet surgery in our center between 2005 and 2012. Average age of 33.2 years and follow up of 75 months. Results were taken by Rowe and Constant scores, DASH questionnaire and we also recorded a subjective assessment of the result by each patient. Results: No recurrence was reported. No reoperations. Mean Rowe score was 74.6 points and mean Constant score was 70 points. In the disability questionnaire (DASH), the mean value was 22.9 points. The outcome assessment by the patients was positive in 78.8%. The migration of a screw occurred in one patient but he still asymptomatic. No other complication was identified. Conclusions: The Bristow-Latarjet technique is a reliable technique, with few complications and with an excellent rate of recurrence in treatment of chronic shoulder instability as reported in literature; it should be used as primary surgery in some cases and the preoperative ISIS score is an excellent and simple guide to select correctly the surgical technique for each patient.

[Bristow-Latarjet Technique for anterior glenohumeral instability]. / Técnica de Bristow-Latarjet en la inestabilidad glenohumeral anterior.

BACKGROUND: In treatment of anterior shoulder instability, many surgical techniques have been described, all of them with advantages and disadvantages. Our goal is to study the half term results on patients that underwent open Bristow-Latarjet surgery considering the preoperative ISIS value. MATERIAL AND METHODS: This is a retrospective study of 33 patients which underwent open Bristow-Latarjet surgery in our center between 2005 and 2012. Average age of 33.2 years and follow up of 75 months. Results were taken by Rowe and Constant scores, DASH questionnaire and we also recorded a subjective assessment of the result by each patient. RESULTS: No recurrence was reported. No reoperations. Mean Rowe score was 74.6 points and mean Constant score was 70 points. In the disability questionnaire (DASH), the mean value was 22.9 points. The outcome assessment by the patients was positive in 78.8%. The migration of a screw occurred in one patient but he still asymptomatic. No other complication was identified. CONCLUSIONS: The Bristow-Latarjet technique is a reliable technique, with few complications and with an excellent rate of recurrence in treatment of chronic shoulder instability as reported in literature; it should be used as primary surgery in some cases and the preoperative ISIS score is an excellent and simple guide to select correctly the surgical technique for each patient.

ANTECEDENTES: En el tratamiento de la inestabilidad glenohumeral anterior se han descrito multitud de técnicas quirúrgicas, todas ellas con ventajas e inconvenientes. Nuestro objetivo es analizar los resultados obtenidos a medio plazo en los pacientes que hemos intervenido mediante la técnica de Bristow-Latarjet teniendo en cuenta el valor del ISIS preoperatorio. MATERIAL Y MÉTODOS: Estudio retrospectivo de 33 pacientes intervenidos entre 2005 y 2012 con edad media de 33.2 años y seguimiento medio de 75 meses. Se recogió el resultado con las escalas de Rowe y Constant, cuestionario DASH y una encuesta de valoración subjetiva del resultado por parte de cada paciente. RESULTADOS: No se presentaron recidivas. La puntuación media postoperatoria en la escala de Rowe fue de 74.6 puntos y de 70 puntos en la escala de Constant. En el cuestionario DASH el valor promedio fue 22.9 puntos. En 78.8% de los pacientes se valoró positivamente el resultado. Se produjo la migración del tornillo en un paciente, encontrándose asintomático. No se identificaron otras complicaciones. CONCLUSIONES: La técnica de Bristow-Latarjet en el tratamiento de la inestabilidad glenohumeral anterior es una técnica fiable y con una mínima tasa de recidivas, por lo que debe emplearse como cirugía de elección en determinados casos y, para ello, la valoración preoperatoria con la escala ISIS es una buena guía a la hora de indicar el tipo de cirugía.

La enseñanza de la enfermería con simuladores, consideraciones teórico-pedagógicas para perfilar un modelo didáctico / Nursing teaching using simulators, theoretical and pedagogical considerations to design a didactic model / O ensino da enfermagem com simuladores, considerações teórico-pedagógicas para perfilar um modelo didático

Introducción: La incorporación de simuladores de alta fidelidad en la enseñanza de la enfermería constituye una práctica educativa que tiende a extenderse en razón de constituir un entorno educativo favorable para el desarrollo de habilidades en los alumnos y paralelamente aportar condiciones éticas de mayor seguridad en el paciente. Desarrollo: La incorporación de estas tecnologías ofrece nuevas posibilidades educativas en la formación de enfermería, pero es preciso revisar las bases teóricas que conforman un modelo pedagógico, así como esclarecer los nuevos roles que adquieren docentes y alumnos en estos nuevos escenarios de enseñanza aprendizaje. La consideración del concepto de aprendizaje experiencial de John Dewey, así como de zona de desarrollo próximo del constructivismo sociocultural de Vygotsky, aportan elementos sugerentes para la construcción de un modelo pedagógico. Estas consideraciones teóricas demandan el desarrollo de nuevas competencias en el docente, como son el diseño de escenarios de enseñanza aprendizaje, su adecuada ejecución y su evaluación, bajo una concepción de aprendizaje que pone en el centro la actividad del alumno. Experiencias educativas que han empleado simuladores de alta fidelidad reportan que si bien en todos los casos se incrementa la habilidad y la seguridad del alumno, pueden resultar poco estimulantes por su falta de realismo. Conclusiones: El uso de simuladores no debe centrarse solamente en la ejercitación de procedimientos manuales, sino generar paralelamente un entorno de aprendizaje en el que se integren el desarrollo de otras habilidades como la comunicación, reflexión, pensamiento crítico, toma de decisiones y la consideración del paciente como un ente humano complejo.

Introduction: The incorporation of high-fidelity simulators in nursing teaching is an educational practice which tends to spread because it promotes the development of skills in students as well as higher ethical conditions of safety for the patient. Development: The incorporation of these technologies offers new educational possibilities for nursing teaching, yet it is necessary to review the theoretical foundations which support pedagogical models, as well as to clarify the new roles which both teachers and students adopt for these new teaching-learning scenarios. The concept of experience learning of John Dewey and the concept of the proximal development zone of Vygotsky contribute to integrate new pedagogical models. These theoretical considerations demand that teachers develop new competences including those related to scenarios design and program execution and assessment, all of these under a learning conception which places the student at the center of the activity. However, some educational programs which have used high-fidelity simulators report that, although there were improvements in student skills and safety, sometimes these practices can be not stimulating enough due to a lack of realism. Conclusions: The use of simulators should not be only centered on manual procedures practices but also on the generation of a learning environment which promotes the development of other skills such as communication, reflection, critical thought, decision taking, and the conception of the patient as a complex human entity.

Introdução: A incorporação de simuladores de alta-fidelidade no ensino da enfermagem constitui uma prática educativa que tende a espalhar-se, com o objetivo de constituir um ambiente educativo favorável para o desenvolvimento de competências nos alunos e paralelamente contribuir às condições éticas de maior segurança no paciente. Desenvolvimento: A incorporação destas tecnologias oferece novas possibilidades educativas de formação da enfermagem, mas é preciso revisar as bases teóricas que conformam um modelo pedagógico, bem como esclarecer os novos papéis que conseguem docentes e alunos nestes novos cenários de ensino-aprendizagem. A consideração do conceito de aprendizagem experiencial de John Dewey, bem como da zona de desenvolvimento próximo do construtivismo de Vygotsky, oferecem elementos provocadores para a construção de um modelo pedagógico. Estas considerações teóricas demandam o desenvolvimento de novas competências no docente, como são o desenho de cenários de ensino-aprendizagem, a sua adequada execução e avaliação, sob uma concepção de aprendizagem que coloca o aluno no centro da atividade. Experiências educativas que têm empregado simuladores de alta-fidelidade reportam que, apesar de aumentarem a competência e a segurança do aluno em todos os casos, podem resultar pouco motivadores pela sua falta de realismo. Conclusões: O uso de simuladores não se deve concentrar somente na exercitação de procedimentos manuais, senão gerar paralelamente um ambiente de aprendizagem no qual se integrem o desenvolvimento de outras competências de comunicação, reflexão, pensamento crítico, tomada de decisões e a consideração do paciente como ser humano complexo.

Fertilization capacity of cryopreserved Iberian ibex epididymal sperm in a heterologous in vitro fertilization assay.

In vitro fertilization (IVF) can be used to assess the fertilization capacity of sperm. Heterologous IVF may be useful when assessing that of wild animals as it is often difficult to obtain adequate numbers of naturally corresponding oocytes. The aim of the present study was to assess the fertilization capacity of frozen-thawed ibex epididymal spermatozoa via heterologous IVF involving the oocytes of prepubertal domestic goats. The effect on fertilization and embryo development of adding oestrous sheep serum (ESS) to the fertilization medium was also examined. Cumulus-oocyte complexes (COCs) were matured in TCM-199 for 24-27 h at 38.5°C in a 5% CO2 in air atmosphere. Frozen-thawed epididymal spermatozoa were selected by density gradient centrifugation. After maturation, the oocytes were co-incubated with spermatozoa in synthetic oviductal fluid (SOF) with different concentrations of ESS: SOF-C (0%), SOF-2 (2%) and SOF-20 (20%). At 17 h post-insemination (hpi), zygotes with one female and one male pronucleus (2PN) were categorised as normal; zygotes with 3PN were recorded as polyspermic, and oocytes with 1PN as asynchronous. Cleavage and blastocyst development were assessed at 48 and 168 hpi respectively. The percentage of zygotes with 2PN was higher in the SOF-2 than in the SOF-20 treatment group (27.7% versus 2.9% P < 0.05). The percentage of blastocysts formed with the SOF-C, SOF-2 and SOF-20 treatments were 1.1%, 7.5% and 0% respectively. These results show that the presence of 2% ESS achieves better results than the use of no serum or the standard 20% concentration. Heterologous IVF may be an effective method for predicting the fertilization capacity of ibex spermatozoa, and therefore perhaps that of other wild mountain ungulates.