Different Ways to Defend Victims of Bullying: Defending Profiles and Their Associations with Adolescents' Victimization Experiences and Depressive Symptoms.

Adolescents use various strategies to help their victimized peers during bullying episodes. However, prior research has primarily adopted a variable-centered approach that examines the effect of each defending strategies separately and does not address whether there were different types of defenders who exhibit specific combinations of defending strategies and how these profiles related to youth's adjustment outcomes. Using latent profile analysis, this study identified defending profiles among a sample of Chinese adolescents (N = 1618, Mage = 13.81, SDage = 0.94, 42% girls) and examined whether these profiles differ on victimization experiences and depressive symptoms. The results yielded four defending profiles: nonaggressive defenders (15%), aggressive defenders (7%), average defenders (54%), and infrequent defenders (24%). Aggressive defenders and infrequent defenders exhibited the highest levels of self-reported victimization and depressive symptoms, whereas nonaggressive defenders demonstrated the lowest. There were no statistical profile differences in peer-reported victimization. Findings suggest that investigating the heterogeneity of youth using defending strategies is important for understanding whether defending actually puts youth at increased risk for negative adjustment.

Relations between Prosociality and Psychological Maladjustment in Chinese Elementary and Secondary School Students: Mediating Roles of Peer Preference and Self-Perceived Social Competence.

Despite empirical findings that prosociality can prevent elementary and secondary school students from developing psychological maladjustment, little is known about the underlying mechanisms. The goal of the present study was to examine the mediating effects of peer preference and self-perceived social competence on the associations between prosociality and psychological maladjustment (i.e., depressive symptoms and loneliness). Participants were 951 students (Mage = 11 years, 442 girls) in Grades 3~7 from Shanghai, China. They completed peer nominations of prosociality and peer preference and self-report measures of self-perceived social competence, depressive symptoms, and loneliness. Multiple mediation analyses revealed that: (a) both peer preference and self-perceived social competence mediated the relations between prosociality and psychological maladjustment, and (b) a serial indirect pathway (i.e., prosociality → peer preference → self-perceived social competence → psychological maladjustment) emerged when controlling for age group and gender. These findings point to potential targets in the prevention and intervention of Chinese students' internalization of problems.

Longitudinal Associations Between Prosociality and Depressive Symptoms in Chinese Children: The Mediating Role of Peer Preference.

Despite empirical findings that prosociality is related to decreased depressive symptoms in children, little is known about the directionality of the relations and the mechanisms that may explain the relations. To address these gaps, this study examined bi-directional associations between prosociality and depressive symptoms and the mediating effects of peer preference on the associations in Chinese children. Multi-wave longitudinal data were collected each year from Grades 3 to 6 in a sample of children in China (initial N = 1012; 51.6% girls; initial Mage = 8.68 years). The results showed that prosociality and depression negatively contributed to each other over time. Prosociality also predicted increased peer preference, which in turn contributed to fewer depressive symptoms, suggesting that peer preference was a mediator of the contributions of prosociality to depressive symptoms. These findings indicate the temporal ordering of prosociality and depressive symptoms and the processes in the development of depressive symptoms in Chinese children.

Overexpression and diagnostic significance of INTS7 in lung adenocarcinoma and its effects on tumor microenvironment.

BACKGROUND: Lung cancer is the leading cause of death worldwide, and lung adenocarcinoma (LUAD) is the most common histological subtype. INTS7, one of the subunits of the integrator complex, is upregulated in several tumors. Thus, we aimed to investigate the expression profile and clinical significance of INTS7 in LUAD. METHODS: The expression profile of INTS7 was tested in TCGA database and clinical specimens. ROC curve was used to detect the diagnostic value of INTS7, CEA and INTS7 combined with CEA. Kaplan-Meier analysis was used to analyze the prognostic value of INTS7. Differentially expressed genes (DEGs) related to INTS7 were analyzed, and functional enrichment analysis was used to explore the potential mechanisms related to DEGs. The correlations between INTS7 and tumor-infiltrating immune cells, immune scores, stromal scores, and immune checkpoints were explored. Finally, the relationship between INTS7 expression and sensitivity to molecular-targeted therapy was examined. RESULTS: Data from TCGA database showed that INTS7 mRNA expression was substantially upregulated in LUAD, the AUC values of INTS7 for diagnosing LUAD were >0.8, combined detection of INTS7 and CEA could improve the diagnostic efficiency and early stage patients with high expression of INTS7 showed shorter overall survival. IHC analysis of clinical samples further verified the overexpression of INTS7 protein and confirmed the diagnostic value of INTS7 in LUAD, especially for patients at advanced stages with the AUC >0.8. A total of 192 DEGs were identified and DEGs were primarily involved in cell cycle, inflammatory response, and immune response. Moreover, INTS7 expression was negatively correlated with memory B cells, regulatory T cells (Treg), monocytes, resting myeloid dentritic cells and activated mast cells infiltration, and positively correlated with naive B cells, T follicular helper cells (Tfh), activated myeloid dentritic cells and neutrophils infiltration. In addition, patients with high expression of INTS7 showed less expression of immune checkpoints and exhibited less sensitivity to molecular-targeted drugs. CONCLUSION: INTS7 is a potential diagnostic biomarker for LUAD. And its expression level may correlate with tumor microenvireoment, immunotherapy responsiveness, and molecular-targeted therapy responsiveness in LUAD.