Apoptosis-like bacterial death modulated by photoactive hyperthermia nanomaterials and enhanced wound disinfection application.

Photothermal therapy (PTT) is considered as an efficient therapeutic strategy for wound disinfection. However, there is a dilemma that on the one hand, the high PTT temperature for killing bacteria (>58 °C) could cause serious injury to normal tissue, however, low-temperature results in unsatisfactory treatment efficiency. To settle the issue, we have proposed a novel approach to gently kill bacteria in an apoptosis-like mode via PTT, in which the bacteria can maintain intact membranes but cannot proliferate. This is different from the typical necrosis-like mode of bacterial cell death requiring higher temperatures. We found that PTT prefers to trigger the gradual efflux of Ca2+/Mg2+ ions from the bacterial intracellular content rather than directly destroy the outer membranes, but can cause the dynamic variation of the membrane surface micromorphology. Hence, the microbial viability of E. coli can be dynamically changed from the live state to an apoptosis-like state (45-55 °C), then to apoptosis/necrosis (ca. 58 °C), and finally to necrosis (>61 °C). Based on this strategy, we can kill bacteria through an apoptosis-like mode. Better healing efficacy of mice wounds was achieved at a PTT temperature of 50 °C as compared to that at 58 °C, which sheds light on the wound disinfection and healing applications in clinics with a mild PTT strategy.

Membrane destruction and phospholipid extraction by using two-dimensional MoS2 nanosheets.

The interaction of two-dimensional (2D) nanomaterials and bacterial membranes has attracted tremendous attention in antibacterial applications. Various peculiarities of 2D nanomaterials may lead to multiple mechanisms of their interactions with membranes. Here, we investigated the interaction between molybdenum disulfide (MoS2) nanosheets and the bacterial membrane by using both theoretical and experimental approaches. Molecular dynamics simulation presented that MoS2 nanosheets can disrupt the structure of the lipid membrane by making dents on its surface and extracting phospholipid molecules to reduce the integrity of the membrane. This is attributed to the combination of the dispersion interaction of lipid tails with S atoms and the electrostatic interactions of lipid head groups with the Mo and S atoms in the lateral edges of the MoS2 nanosheet. Scanning electron microscopy and transmission electron microscopy confirmed the dents and the destruction of the cell membrane, which would lead to the loss of cytoplasm and the death of bacteria. It should be noted that the phenomenon where MoS2 induces a dent is different from the direct insertion of graphene-based nanomaterials, which might be due to the thicker and stiffer structure of MoS2. Therefore, we believe that the molecular interactions of 2D nanomaterials with bacterial membranes should be highly correlated with their structural characteristics. This newly discovered mechanism of MoS2 nanomaterials to disrupt the cell membrane may promote the application of transition metal dichalcogenide (TMD) nanomaterials in designing remarkable antibacterial materials in the near future.