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Objective: To study the effect of Matrilin-4 on the reparative dentin formation in the rats after dental pulp injury, and to explore its possibility to be used as a new capping agent. Methods: A total of 28 male Wistar rats were selected. A cavity on the occlusal surface of maxillary first molars on both sides was prepared in each rat. Matrilin-4 was applied on the left exposed pulp (Matrilin-4 group), and PBS was applied on the right exposed pulp (PBS group); and the cavities in both sides were covered with glass ions. On days 3, 7, 14 and 28 after operation, the rats were sacrificed, and the maxillary first molars on both sides of the rats were selected. HE staining and immunohistochemistry staining were used to observe and analyze the reparative dentin formation and the expression of dentin sialoprotein (DSP) in the odontoblasts. Results: The HE staining results showed that there were less inflammatory cells under the exposed pulp in that Matrilin-4 group at 3 d after operation compared with PBS group, and obvious vasodilation was found. At 7 d after operation, the inflammatory reaction under the exposed pulps in two groups was aggravated, but the inflammatory reaction in Matrilin-4 group was significantly lighter than that in PBS group, and the prophase dentin formation was found. After injury of 14 d, the pulp-exposed areas were covered by more complete dentin bridge in Matrilin-4 group; at 28 d after operation, compared with PBS group, there was a thick, complete and reparative dentin bridge under the exposed pulps, composed of tubular dentin in Matrilin-4 group, and there were reorganized odontoblastic layers beneath the dentin bridge. The results of immunohistochemistry showed that the positive expression strengths of DSP in the odontoblasts of the rats in two groups were gradually increased at 3, 7 and 14 d after operation, and were decreased at 28 d. The positive expression strengths of DSP in the odontoblast of the rats in Matrilin-4 group were stronger than those in PBS group at each time point after operation, and the positive expression strength reached the peak at 14 d. Conclusion: Compared with PBS group, the positive expression strength of DSP in the odontoblasts of the rats in Matrilin-4 group had significant differences (P<0.01) at 3, 7 and 14 d.
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Objective:To repaire the posterior tooth defects in the elderly people with CEREC chairside CAD/CAM restorations,and to evaluate the clinical curative effect 1 year after treatment.Methods:A total of 52 posterior teeth from 48 aged patients were selected.Among them,24 cases were onlays,18 cases were inlays,and 10 cases were crowns.The inlays/onlays/crowns were designed and manufactured by CEREC chairside CAD/CAM system,and then bonded with resin cement.After 12 months,the patients were followed up.The teeth with restorations were assessed by using the modified USPHS criteria including restorations,tooth,and periodontal.At the same time,the patient's satisfaction was evaluated.Results:In all of the 52 restorations,the success rates of inlay,onlay and crown were 94.4%,91.7% and 80.0%,respectively;among them,one inlay was fractured,one onlay did not close the edge,one onlay's edge integrity was mild defect,one teeth was fractured,one crown's adjacent relationship was not close,and one crown appeared papillitis.Conclusion:The restorations manufactured by CEREC chairside CAD/ CAM system have a good short-term clinical curative effect.It is an effective way to repair the posterior tooth defects in the elderly people.
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Objective:To report one case of bilateral maxillary fourth molars,and to explore the occurrence and treatment method of additional fourth molar.Methods:The case materials of one patient with bilateral maxillary four molars were collected,and the information was record.Combined with reviewing the relevant literatures,the clinical data,complication and treatment of one patient with bilateral maxillary fourth molars were retrospectively analyzed.Results:The patient was diagnosed due to left maxillary posterior teeth area intermittent pain for several months and the increased pain for 5 d;at the same time,there was no abnormal appearance inducing the symptoms detected by speciality check-up.The pantomography showed the four molars in bilateral maxillary,and the fourth molars impacted the third molar root's absorption.The fourth molar crown was smaller and the root was shorter. The 18,28,29 teeth were removed,and the left maxillary posterior teeth area intermittent pain disppeared. Conclusion:The occurrence of additional fourth molar is a relatively rare demal dysplasia.However,there are some complications following with the fourth molar.Early diagnosis and treatment are essential.
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Objective: To report one case of bilateral maxillary fourth molars, and to explore the occurrence and treatment method of additional fourth molar. Methods: The case materials of one patient with bilateral maxillary four molars were collected, and the information was record. Combined with reviewing the relevant literatures, the clinical data, complication and treatment of one patient with bilateral maxillary fourth molars were retrospectively analyzed. Results: The patient was diagnosed due to left maxillary posterior teeth area intermittent pain for several months and the increased pain for 5 d; at the same time, there was no abnormal appearance inducing the symptoms detected by speciality check-up. The pantomography showed the four molars in bilateral maxillary, and the fourth molars impacted the third molar root's absorption. The fourth molar crown was smaller and the root was shorter. The 18, 28, 29 teeth were removed, and the left maxillary posterior teeth area intermittent pain disppeared. Conclusion: The occurrence of additional fourth molar is a relatively rare demal dysplasia. However, there are some complications following with the fourth molar. Early diagnosis and treatment are essential.
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Objective:To explore the etiology and treatment of one case of bilateral mandibular second molar impaction with paradental cyst, and to provide a reference for its diagnosis and treatment. Methods:Root canal treatment of the left mandibular first molar of the patient was performed before operation.The left mandibular second molar of the patient was removed;the residual dental follicle, the granulation tissue and the cyst wall were stroken off under local anesthesia.The diamond ball was used to polish the wound cavity and sharp bone edge, and to mill the distal apical part of left mandibular first molar.The tissue removed during the procedure was used for the pathological examination.Results:The X-ray image showed that the bilateral mandibular second molar was impacted with the left mandibular first molar root's absorption, and there was a clear round-like density reduction zone around the second molar crown.The pathologic result was paradental cyst.Conclusion:Dental impaction complicated with paradental cyst could occur in other tooth position except for the third molar.Its diagnosis should be combined with the clinical manifestations, the pathologic manifestations and the medical imaging.Multidisciplinary consultation is in favor of its diagnosis and treatment.