Bovine DDX3X Restrains Bovine SP110c-Mediated Activation of Inflammasome in Macrophages.

The inflammasome is a vital part of the host's innate immunity activated by cellular infection or stress. Our previous research identified the bovine SP110c isoform (bSP110c) as a novel activator of the inflammasome that promoted the secretion of proinflammatory cytokines IL-1ß and IL-18 in macrophages infected with Listeria monocytogenes or stimulated with lipopolysaccharide (LPS). However, the exact molecular mechanism for inhibiting bSP110c-induced inflammasome activation requires further clarification. Here, the researchers identified bovine DDX3X (bDDX3X) as an NLRP3-associated protein and an inhibitor of the bSP110c-induced inflammasome in the human THP1 macrophage cell line. Immunoprecipitation showed that bDDX3X interacted with the bSP110c CARD domain via its helicase domain. The co-expression of bSP110c and bDDX3X in THP1 macrophages significantly prevented the bSP110c-induced activation of inflammasomes. In addition, both bDDX3X and bSP110c interacted with bovine NLRP3 (bNLRP3), and bDDX3X enhanced the interaction between bSP110c and bNLRP3. The expression of bDDX3X in nigericin-stimulated THP1 macrophages significantly suppressed NLRP3 inflammasome activation, ASC speck formation, and pyroptosis. These findings demonstrate that bDDX3X negatively regulates the bSP110c-mediated inflammatory response by restricting the activation of the NLRP3 inflammasome. This discovery unveils a novel regulatory mechanism involving bDDX3X and bSP110c in coordinating inflammasome activation and subsequent cell-fate decisions in LPS-treated macrophages and, in turn, constitutes a step forward toward the implementation of marker-assisted selection in breeding programs aimed at utilizing cattle's immune defenses.

Strategies for tailoring functional microbial synthetic communities.

Natural ecosystems harbor a huge reservoir of taxonomically diverse microbes that are important for plant growth and health. The vast diversity of soil microorganisms and their complex interactions make it challenging to pinpoint the main players important for the life support functions microbes can provide to plants, including enhanced tolerance to (a)biotic stress factors. Designing simplified microbial synthetic communities (SynComs) helps reduce this complexity to unravel the molecular and chemical basis and interplay of specific microbiome functions. While SynComs have been successfully employed to dissect microbial interactions or reproduce microbiome-associated phenotypes, the assembly and reconstitution of these communities have often been based on generic abundance patterns or taxonomic identities and co-occurrences but have only rarely been informed by functional traits. Here, we review recent studies on designing functional SynComs to reveal common principles and discuss multidimensional approaches for community design. We propose a strategy for tailoring the design of functional SynComs based on integration of high-throughput experimental assays with microbial strains and computational genomic analyses of their functional capabilities.

Bulk Modification of Porous TiNb2 O7 Microsphere to Achieve Superior Lithium-Storage Properties at Low Temperature.

TiNb2 O7 , as a promising alternative of Li4 Ti5 O12 , exhibits giant potential as low-temperature anode due to its higher theoretical capacity and comparable structural stability. However, the sluggish electronic conductivity still remains a challenge. Herein, bulk modification of Cu+ doping in porous TiNb2 O7 microsphere is proposed via a simple one-step solvothermal method with subsequent calcination treatment. The results show that the electronic conductivity is improved effectively due to the reduced band gap after doping, while enhanced lithium-ion diffusion is achieved benefiting from the increased interplanar spacing. Therefore, the optimal sample of Cu0.06 Ti0.94 Nb2 O7 exhibits a high reversible capacity of 244.4 mA h g-1 at 100 mA g-1 after 100 cycles, superior rate capability, and long-term cycling stability at 1000 mA g-1 at room temperature. Particularly, it can also display good performance in a wide temperature range from 25 to -30 °C, including a reversible capacity of 76.6 mA h g-1 at -20 °C after 200 cycles at 200 mA g-1 . Moreover, Cu0.06 Ti0.94 Nb2 O7 //LiFePO4 full cell can deliver a high reversible capacity of 177.5 mA h g-1 at 100 mA g-1 . The excellent electrochemical properties at both ambient and low-temperatures demonstrate the great potential of Cu+ -doped TiNb2 O7 in energy-storage applications.

A core epiphytic bacterial consortia synergistically protect citrus from postharvest disease.

Biological antagonists are a series of microbes that can control pathogens to reduce the incidence of disease or reduce symptoms. Herein, four varieties of citrus fruit were selected to perform an amplicon sequencing on their epiphytic microbiota to get a systematic understanding of them. Co-occurrence network, Venn, and LefSe analysis were performed to filter to 24 genera which represent the universality, specificity, and correlation among samples. Functional analysis hinted that the genes related to chitinase, which most of these 24 bacteria carry, might lead to a disease-suppressive phenotype. 115 strains of epiphytic bacteria were isolated, and the bacterial synthetic community was constructed by 8 strains. The in vivo test results indicated they were able to reduce pathogen development for a longer time than separate inoculation. Collectively, this study showed the disease control potential provided by native epiphytic bacteria of fruit and give a new strategy to sustainable agriculture.