RESUMEN
The paper introduces professor GAO Shu-zhong's understanding on "seeking yin from yang needling method" and its clinical application on the basis of "qi street" and "four seas" theories. Through professor GAO's clinical practice for years, he integrates and extendes the theories of "seeking yin from yang", "qi street" and "four seas" in Huangdi Neijing (The Yellow Emperor's Inner Classic). In this specific acupuncture method, in reference with the theories of "qi street" and "four seas", acupuncture is exerted on yang part of body, e.g. the back and lumber region to treat the diseases of yin parts, e.g. the chest and abdomen, which is differentiated as yin-yang imbalance in pathogenesis. In order to fully explain the clinical curative effect of "seeking yin from yang needling method", the common diseases in clinic, e.g. the disorders of heart, spleen and stomach systems, as well as the gynecology are taken as examples in the paper.
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Humanos , Masculino , Acupuntura , Terapia por Acupuntura/historia , Qi , Procedimientos Quirúrgicos Vasculares , Yin-YangRESUMEN
The paper introduces professor GAO Shu-zhong's experience in qiguan (umbilicus pass) theory and its clinical application. Professor GAO believes that the umbilicus is the "pass" where the primary qi of sanjiao transported from the lower jiao to the middle jiao. It is the general pivot of qi transformation of yin and yang, as well as the place for qi ascending, descending, exiting and entering in the human body. Hence, the umbilicus is called qiguan (umbilicus pass). In clinical practice, associated with observation, palpation and pulse diagnosis, the qiguan theory is conductive to disease diagnosis. Moreover, the therapeutic methods for promoting qiguan is generated, i.e. umbilicus-acupuncture therapy and umbilicus-moxibustion therapy. In the umbilicus-acupuncture therapy, Gao 's umbilicus five points (umbilicus heart, umbilicus stomach, umbilicus liver, umbilicus kidney and umbilicus lung) are commonly selected. With the umbilicus- moxibustion therapy, the isolated moxibustion with different herbal materials is exerted at the umbilicus, in which, the herbal materials with drastic medical action, pungent and fragrant in flavor and warm in property are specially selected.
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Humanos , Puntos de Acupuntura , Terapia por Acupuntura , Moxibustión , Estómago , OmbligoRESUMEN
Thyroid-associated ophthalmopathy (TAO) belongs to autoimmun diseases, which is particularly closely related to the liver, spleen and kidney. Using these three organs as the theoretical foundation, the root of the disease can be grasped. Using syndrome differentiation, deficiency is the basic pattern and excess is the syndrome. The main reason for TAO is liver, spleen and kidney deficiency, while dampness, phlegm-blood stagnation in the eyes are pathogen. The rules of treatments are nourishing yin and clearing liver, supplementing and nourishing liver and kidney, removing dampness and phlegm, eliminating blood stasis and promoting circulation. By combining overall symptoms with ocular region changes, together with dynamic differentiation and flexible treatment, satisfactory results have been obtained in clinical practice.
RESUMEN
OBJECTIVE: To investigate the effects of guavenoic acid(GA) on protection against oxidative damage in INS-1 cells. METHODS: INS-1β cells were cultured in vitro. Control group, medium group, model group, drug groups and positive group were set. INS-1 cells were treated with GA in 0.3, 1, 3, 10, 30 nmol · L-1 for 48 h. Using 650 μmol · L-1 H2O2 challenges the INS-1 cells for 2 h to establish the oxidative damage model(OD), the cell viability was tested by MTT assay, the nuclear was stained by Hoechst 33258, the ROS in OD-INS-1 was tested by ROS-kit and the apoptosis was tested via double staining-fcm. RESULTS: Compared with model group, at low concentration GA(0.3, 1 nmol · L-1) could protect INS-1 cells exposed to H2O2 significantly (P -1 GA, and emitting weaker cell fluorescence. 0.3, 1, 3 nmol · L-1 GA could reduce the DCF fluorescence intensity very significantly (P -1, GA can significantly resist the occurrence of apoptosis(P < 0.05). CONCLUSION: GA could protect INS-1 cells from oxidative damage, maybe achieved by inhibiting intracellular ROS generation and against apoptosis.