RESUMEN
BACKGROUND: Recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in blood and can serve as useful biomarkers for cancer. METHODS: We performed an miRNA array using serum samples obtained from oesophageal squamous cell carcinoma (ESCC) patients or healthy controls. MiR-1246 was the most markedly elevated in ESCC patients. Therefore, miR-1246 was selected as a candidate for further analysis. The serum miR-1246 level in 46 healthy controls and 101 ESCC patients was evaluated and compared among various clinicopathological characteristics. MiR-1246 expressions in tissue, exosomal, and cellular samples were also examined. RESULTS: Serum miR-1246 alone yielded an receiver-operating characteristic curve area of 0.754, with 71.3% sensitivity and 73.9% specificity for distinguishing ESCC patients from healthy controls. Serum miR-1246 was significantly correlated with the TNM stage and showed to be the strongest independent risk factor for poor survival (HR, 4.032; P=0.017). Unlike the tendency shown in previous reports, miR-1246 was not upregulated in ESCC tissue samples. Furthermore, exosomal miR-1246 did not reflect the abundance in the cell of origin. CONCLUSION: These data support our contention that serum miR-1246 has strong potential as a novel diagnostic and prognostic biomarker in ESCC, and its releasing mechanism is selective and independent of tissue miRNA abundance.
Asunto(s)
Adenocarcinoma/genética , Biomarcadores de Tumor/sangre , Neoplasias Esofágicas/genética , Esófago/metabolismo , Perfilación de la Expresión Génica , MicroARNs/sangre , Adenocarcinoma/sangre , Adenocarcinoma/patología , Anciano , Biomarcadores de Tumor/genética , Estudios de Casos y Controles , Neoplasias Esofágicas/sangre , Neoplasias Esofágicas/patología , Exosomas/metabolismo , Femenino , Humanos , Metástasis Linfática , Masculino , MicroARNs/genética , Metástasis de la Neoplasia , Estadificación de Neoplasias , Análisis de Secuencia por Matrices de Oligonucleótidos , Pronóstico , ARN Mensajero/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa InversaAsunto(s)
Anticonceptivos Orales , Servicios de Planificación Familiar , Regulación Gubernamental , Aborto Legal , Síndrome de Inmunodeficiencia Adquirida/transmisión , Condones , Anticonceptivos Orales/administración & dosificación , Anticonceptivos Orales/efectos adversos , Aprobación de Drogas , Femenino , Conocimientos, Actitudes y Práctica en Salud , Humanos , Internacionalidad , Japón , Legislación de Medicamentos , Masculino , Mifepristona/administración & dosificación , Embarazo , Medición de RiesgoRESUMEN
Two cases of leiomyoma of the bladder are reported. The first case was a 79-year-old female who presented with gross hematuria and frequent urination. Cystoscopy disclosed a large tumor covered by intact mucosa. This lesion was further evaluated by ultrasound, and was thought to be a submucosal tumor of the bladder. The second case was a 43-year-old female who was referred for further investigation of left giant hydronephrosis. During the investigation, submucosal tumor was incidentally found. Concerning the etiology of the hydronephrosis, left ureteral stenosis due to a previous gynecological operation was suspected. These lesions proved to be leiomyoma of the bladder histologically, and were successfully enucleated. Difficulty in diagnosis of this disease and controversy as to the optimal treatment prompted us to report these cases and to review 68 cases reported in the Japanese literature.