RESUMEN
Although memory impairments are a hallmark of aging, the degree of deficit varies across animal models, and is likely to reflect different states of deterioration in metabolic and endocrinological properties. This study investigated memory-related processes in young (3-4 months) and old (24 months) Sprague-Dawley rats (SD), which develop age-linked pathologies such as obesity or insulin-resistance and Lou/C/Jall rats, which do not develop such impairments. In short- and long-term memory recognition tasks, old Lou/C/Jall rats were never impaired whereas old SD rats were deficient at 1 and 24h latencies. The expression of N-methyl-d-aspartate receptors (NMDAR)-mediated synaptic plasticity in CA1 hippocampal networks shifted towards lower activity values in old Lou/C/Jall rats whereas long-term potentiation was impaired in age-matched SD rats. Age-related decrease in NR2A subunits occurred in both strains, extended to NR2B, NR1 and GluR1 subunits in older animals (28 months) but only in SD rats. Therefore, the Lou/C/Jall rats can be considered as a model of healthy aging, not only in terms of its preserved metabolism, but also in terms of cognition and synaptic plasticity.
Asunto(s)
Envejecimiento/metabolismo , Hipocampo/metabolismo , Trastornos de la Memoria/metabolismo , Plasticidad Neuronal/fisiología , Receptores de N-Metil-D-Aspartato/metabolismo , Envejecimiento/genética , Animales , Predisposición Genética a la Enfermedad , Hipocampo/fisiopatología , Resistencia a la Insulina/genética , Masculino , Trastornos de la Memoria/genética , Trastornos de la Memoria/fisiopatología , Memoria a Corto Plazo/fisiología , Obesidad/complicaciones , Obesidad/genética , Obesidad/metabolismo , Técnicas de Cultivo de Órganos , Ratas , Ratas Endogámicas , Ratas Sprague-Dawley , Receptores AMPA/metabolismo , Especificidad de la EspecieRESUMEN
Activation of N-methyl-D-aspartate receptors (NMDARs) is the first step in the induction of certain forms of synaptic plasticity in the hippocampus. In the adult rat hippocampus, NMDARs are composed almost exclusively of NR1 and NR2 subunits with NR1 subunits being mainly associated with either NR2A and/or NR2B subunits. The role played by the different subunits in synaptic plasticity is still controversial. In the present study, we used two different long term depression (LTD) -inducing protocols (electrical and chemical stimulation) to show that activation of NR2A-containing NMDAR subunits leads to the induction of LTD. We also demonstrated that extrasynaptic NR2B-containing NMDARs regulate the magnitude of LTD by exerting a control over the function of synaptic NR2A-containing NMDARs while having no effect on plasticity in the absence of synaptic receptor activation. Taken as a whole, these experiments demonstrate that NMDAR subunits play different roles according to their nature (NR2A or NR2B) and location (synaptic versus extrasynaptic). This sheds new light on the functional role of extrasynaptic NR2B containing-NMDARs. These results are particularly important for a better understanding of certain pathological disorders associated with glutamatergic overactivity.
Asunto(s)
Hipocampo/fisiología , Depresión Sináptica a Largo Plazo/fisiología , Receptores de N-Metil-D-Aspartato/fisiología , Sinapsis/fisiología , Animales , Electrofisiología , Antagonistas de Aminoácidos Excitadores/farmacología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Hipocampo/efectos de los fármacos , Depresión Sináptica a Largo Plazo/efectos de los fármacos , Plasticidad Neuronal/efectos de los fármacos , Plasticidad Neuronal/fisiología , Técnicas de Cultivo de Órganos , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/efectos de los fármacos , Sinapsis/efectos de los fármacosRESUMEN
The aim of this study was to determine whether age-associated alterations in the GABAergic input to pyramidal neurons in the hippocampus are due to a dysfunction of GABAergic interneurons, and/or a decrease in their cholinergic control via nicotinic receptors (nAChRs). Electrophysiological recordings were obtained from pyramidal cells in the CA1 area of hippocampal slices from young (3-4 months old) and aged (25-30 months old) Sprague-Dawley rats. Synaptic GABA(A) receptor-mediated inhibitory postsynaptic currents and inhibitory postsynaptic potentials induced by stimulation of the stratum oriens were significantly smaller in aged rats. The frequency (but not amplitude) of spontaneous and miniature GABA inhibitory postsynaptic currents (IPSCs) was reduced in aged rats, suggesting a presynaptic alteration. Tetanic stimulation of cholinergic afferents to release endogenous acetylcholine, or an exogenous application of the nAChR agonist cytisine, increased the frequency of spontaneous IPSCs in young rats; however these effects were not evident in aged rats, indicating that the nicotinic control of GABA release is lowered during aging. None of these age-related alterations were reversed by a chronic treatment with donepezil, a cholinesterase inhibitor. Immunofluorescent labeling of GABA interneurons with somatostatin (SOM), parvalbumin (PV) or calbindin (CB), together with the vesicular acetylcholine transporter VAChT, revealed a selective loss of subpopulations of SOM and CB positive interneurons. This loss was associated with a general decrease in density of the cholinergic network in aged rats. Thus, the lower GABAergic inhibition observed in the aged rat hippocampus is due to a selective loss/dysfunction of subpopulations of GABAergic interneurons, associated with a widespread cholinergic deficit.
Asunto(s)
Envejecimiento/fisiología , Hipocampo/citología , Células Piramidales/fisiología , Receptores Nicotínicos/fisiología , Ácido gamma-Aminobutírico/metabolismo , Factores de Edad , Alcaloides/farmacología , Análisis de Varianza , Animales , Azocinas/farmacología , Bicuculina/farmacología , Calbindinas , Relación Dosis-Respuesta en la Radiación , Interacciones Farmacológicas , Estimulación Eléctrica/métodos , Antagonistas de Aminoácidos Excitadores/farmacología , Antagonistas del GABA/farmacología , Inmunohistoquímica/métodos , Potenciales Postsinápticos Inhibidores/efectos de los fármacos , Potenciales Postsinápticos Inhibidores/fisiología , Potenciales Postsinápticos Inhibidores/efectos de la radiación , Antagonistas Nicotínicos/farmacología , Parvalbúminas/metabolismo , Técnicas de Placa-Clamp , Células Piramidales/efectos de los fármacos , Células Piramidales/efectos de la radiación , Quinolizinas/farmacología , Ratas , Ratas Sprague-Dawley , Proteína G de Unión al Calcio S100/metabolismo , Somatostatina/metabolismo , Proteínas de Transporte Vesicular de Acetilcolina/metabolismoRESUMEN
Age-associated deficits in learning and memory are closely correlated with impairments of synaptic plasticity. Analysis of N-methyl-D-aspartate receptor (NMDAr)-dependent long-term potentiation (LTP) in CA1 hippocampal slices indicates that the glial-derived neuromodulator D-serine is required for the induction of synaptic plasticity. During aging, the content of D-serine and the expression of its synthesizing enzyme serine racemase are significantly decreased in the hippocampus. Impaired LTP and NMDAr-mediated synaptic potentials in old rats are rescued by exogenous D-serine. These results highlight the critical role of glial cells and presumably astrocytes, through the availability of D-serine, in the deficits of synaptic mechanisms of learning and memory that occur in the course of aging.
Asunto(s)
Envejecimiento/fisiología , Aprendizaje/fisiología , Memoria/fisiología , Neuroglía/metabolismo , Neurotransmisores/metabolismo , Serina/metabolismo , Animales , Sitios de Unión , Hipocampo/citología , Hipocampo/efectos de los fármacos , Potenciación a Largo Plazo/efectos de los fármacos , Masculino , Neurotransmisores/biosíntesis , Neurotransmisores/farmacología , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/metabolismo , Serina/biosíntesis , Serina/farmacología , Transmisión Sináptica/efectos de los fármacos , Transmisión Sináptica/fisiología , Factores de TiempoRESUMEN
The contribution of the cytosolic calcium binding protein calbindin D(28K) (CaBP) to the synaptic plasticity was investigated in hippocampal CA1 area of wild-type and antisense transgenic CaBP-deficient mice. We showed that long-term potentiation (LTP) induced by tetanic stimulation in CaBP-deficient mice was impaired. The fundamental biophysical properties of NMDA receptors and their number were not modified in CaBP-deficient mice. We also demonstrated that the physiological properties of calcium channels were identical between genotypes. An insufficient Ca(2+) entry through NMDA receptors or calcium channels, or a decrease in NMDA receptor density are unlikely to explain this impairment of LTP. Interestingly, we showed that the loss of LTP was not prevented by glycine but was restored in the presence of a low concentration of the NMDA receptor antagonist D-APV (5 microM) and of the calcium chelator BAPTA-AM (5 microM). Moreover, we observed a loss of LTP in the wild-type mice when the postsynaptic tetanic-induced [Ca(2+)](i) rise is excessively increased. Conversely, a weaker tetanus stimulation allowed LTP induction and maintenance in CaBP-deficient mice. These results suggest that a higher cytosol [Ca(2+)](i), due to the decrease of CaBP expression may impair LTP induction and maintenance mechanisms without affecting the mechanisms of calcium entry. Thus, CaBP plays a critical role in long term synaptic plasticity by limiting the elevation of calcium rise in the cytosol to some appropriate spatio-temporal pattern.
Asunto(s)
Canales de Calcio/fisiología , Potenciación a Largo Plazo/genética , Receptores de N-Metil-D-Aspartato/fisiología , Proteína G de Unión al Calcio S100/genética , Animales , Sitios de Unión/genética , Calbindinas , Canales de Calcio/genética , Canales de Calcio/metabolismo , Masculino , Ratones , Ratones Transgénicos , Plasticidad Neuronal/genética , Receptores de N-Metil-D-Aspartato/genética , Receptores de N-Metil-D-Aspartato/metabolismo , Proteína G de Unión al Calcio S100/metabolismo , Proteína G de Unión al Calcio S100/fisiologíaRESUMEN
BACKGROUND: The genetic basis of most common forms of human paroxysmal disorders of the central nervous system, such as epilepsy, remains unidentified. Several animal models of absence epilepsy, commonly accompanied by ataxia, are caused by mutations in the brain P/Q-type voltage-gated calcium (Ca(2+)) channel. We aimed to determine whether the P/Q-type Ca(2+) channel is associated with both epilepsy and episodic ataxia type 2 in human beings. METHODS: We identified an 11-year-old boy with a complex phenotype comprising primary generalised epilepsy, episodic and progressive ataxia, and mild learning difficulties. We sequenced the entire coding region of the gene encoding the voltage-gated P/Q-type Ca(2+) channel (CACNA1A) on chromosome 19. We then introduced the newly identified heterozygous mutation into the full-length rabbit cDNA and did detailed electrophysiological expression studies of mutant and wild type Ca(2+) channels. FINDINGS: We identified a previously undescribed heterozygous point mutation (C5733T) in CACNA1A. This mutation introduces a premature stop codon (R1820stop) resulting in complete loss of the C terminal region of the pore-forming subunit of this Ca(2+) channel. Expression studies provided direct evidence that this mutation impairs Ca(2+) channel function. Mutant/wild-type co-expression studies indicated a dominant negative effect. INTERPRETATION: Human absence epilepsy can be associated with dysfunction of the brain P/Q-type voltage-gated Ca(2+) channel. The phenotype in this patient has striking parallels with the mouse absence epilepsy models.
Asunto(s)
Canales de Calcio/genética , Epilepsia Tipo Ausencia/genética , Ataxia de la Marcha/genética , Adulto , Encéfalo/metabolismo , Canales de Calcio Tipo N , Canales de Calcio Tipo P , Canales de Calcio Tipo Q , Cromosomas Humanos Par 19 , Epilepsia Tipo Ausencia/complicaciones , Ataxia de la Marcha/complicaciones , Humanos , Masculino , Fenotipo , Mutación PuntualRESUMEN
The past few years have seen the elucidation of several neurological diseases caused by inherited mutations of ion channels. In contrast to many other types of genetic disorders, the "channelopathies" can be studied with high precision by applying electrophysiological methods. This review evaluates the success of this approach in explaining the mechanisms of two forms of episodic ataxia that are known to be caused by mutations of ion channels: episodic ataxia type 1 (EA1, caused by K+ channel mutations) and episodic ataxia type 2 (EA2, caused by Ca2+ channel mutations). Although both of these disorders are rare, they raise many important questions about the roles of identified channels in brain function. Indeed, a resolution of the mechanisms by which both diseases occur will represent a major milestone in understanding diseases of the CNS, in addition to opening the way to novel possible treatments.
Asunto(s)
Ataxia/genética , Ataxia/fisiopatología , Ataxia Cerebelosa/fisiopatología , Mutación , Canales de Calcio/genética , Ataxia Cerebelosa/clasificación , Ataxia Cerebelosa/genética , Humanos , Canales de Potasio/genéticaRESUMEN
The electrophysiological and pharmacological properties of alpha(1E)-containing Ca(2+) channels were investigated by using the patch-clamp technique in the whole cell configuration, in HEK 293 cells stably expressing the human alpha(1E) together with alpha(2b) and beta(1b) accessory subunits. These channels had current-voltage (I-V) characteristics resembling those of high-voltage-activated (HVA) Ca(2+) channels (threshold at -30 mV and peak amplitude at +10 mV in 5 mM Ca(2+)). The currents activated and deactivated with a fast rate, in a time- and voltage-dependent manner. No difference was found in their relative permeability to Ca(2+) and Ba(2+). Inorganic Ca(2+) channel blockers (Cd(2+), Ni(2+)) blocked completely and potently the alpha(1E,)/alpha(2b)delta/beta(1b) mediated currents (IC(50) = 4 and 24.6 microM, respectively). alpha(1E)-mediated currents inactivated rapidly and mainly in a non-Ca(2+)-dependent manner, as evidenced by the fact that 1) decreasing extracellular Ca(2+) from 10 to 2 mM and 2) changing the intracellular concentration of the Ca(2+) chelator 1. 2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid (BAPTA), did not affect the inactivation characteristics; 3) there was no clear-cut bell-shaped relationship between test potential and inactivation, as would be expected from a Ca(2+)-dependent event. Although Ba(2+) substitution did not affect the inactivation of alpha(1E) channels, Na(+) substitution revealed a small but significant reduction in the extent and rate of inactivation, suggesting that besides the presence of dominant voltage-dependent inactivation, alpha(1E) channels are also affected by a divalent cation-dependent inactivation process. We have analyzed the Ca(2+) currents produced by a range of imposed action potential-like voltage protocols (APVPs). The amplitude and area of the current were dependent on the duration of the waveform employed and were relatively similar to those described for HVA calcium channels. However, the peak latency resembled that obtained for low-voltage-activated (LVA) calcium channels. Short bursts of APVPs applied at 100 Hz produced a depression of the Ca(2+) current amplitude, suggesting an accumulation of inactivation likely to be calcium dependent. The human alpha(1E) gene seems to participate to a Ca(2+) channel type with biophysical and pharmacological properties partly resembling those of LVA and those of HVA channels, with inactivation characteristics more complex than previously believed.
Asunto(s)
Canales de Calcio/fisiología , Activación del Canal Iónico/fisiología , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Bario/farmacocinética , Tampones (Química) , Cadmio/farmacología , Calcio/farmacocinética , Bloqueadores de los Canales de Calcio/farmacología , Células Cultivadas , Quelantes/farmacología , Ácido Egtácico/análogos & derivados , Ácido Egtácico/farmacología , Humanos , Activación del Canal Iónico/efectos de los fármacos , Riñón/citología , Cinética , Níquel/farmacología , Técnicas de Placa-Clamp , Proteínas Recombinantes , Sodio/farmacocinética , omega-Agatoxina IVA/farmacología , omega-Conotoxina GVIA/farmacologíaRESUMEN
The contribution of the cytosolic calcium binding protein calbindin D(28K) (CaBP) to glutamatergic neurotransmission and synaptic plasticity was investigated in hippocampal CA1 area of wild-type and antisense transgenic CaBP-deficient mice, with the use of extracellular recordings in the ex vivo slice preparation. The amplitude of non-N-methyl-D-aspartate receptor (non-NMDAr)-mediated extracellular field excitatory postsynaptic potentials (fEPSPs) recorded in control medium was significantly greater in CaBP-deficient mice, whereas the afferent fiber volley was not affected. In contrast, the amplitude of NMDAr-mediated fEPSPs isolated in a magnesium-free medium after blockade of non-NMDAr and GABAergic receptors was significantly depressed in these animals. No alteration in the magnitude of paired-pulse facilitation was found, indicating that the presynaptic calcium mechanisms controlling glutamate release were not altered in CaBP-deficient mice. The magnitude and time course of the short-term potentiation (STP) of fEPSPs induced by a 30 Hz conditioning stimulation, which was blocked by the NMDAr antagonist 2-amino-5-phosphonovalerate acid (2-APV), was not impaired in the transgenic mice, whereas long-term potentiation (LTP) induced by a 100 Hz tetanus was not maintained. The long-term depression (LTD) induced by low-frequency stimulation (1 Hz, 15 min) in the presence of the GABA antagonist bicuculline was not altered. These results argue for a contribution of CaBP to the mechanisms responsible for the maintenance of long-term synaptic potentiation, at least in part by modulating the activation of NMDA receptors.
Asunto(s)
Potenciales Postsinápticos Excitadores/fisiología , Hipocampo/química , Potenciación a Largo Plazo/fisiología , Proteínas del Tejido Nervioso/análisis , Receptores de N-Metil-D-Aspartato/fisiología , Proteína G de Unión al Calcio S100/análisis , Sinapsis/química , Transmisión Sináptica/fisiología , 6-Ciano 7-nitroquinoxalina 2,3-diona/farmacología , Animales , Calbindinas , Antagonistas de Aminoácidos Excitadores/farmacología , Hipocampo/fisiología , Masculino , Ratones , Ratones Transgénicos , Proteínas del Tejido Nervioso/deficiencia , Proteínas del Tejido Nervioso/genética , Receptores de N-Metil-D-Aspartato/química , Receptores de N-Metil-D-Aspartato/efectos de los fármacos , Proteína G de Unión al Calcio S100/genéticaRESUMEN
Synaptic responses mediated by the N-methyl-D-aspartate receptor (NMDAr) and non-NMDAr activation were compared in CA1 hippocampal region of young (3-4 months old) and aged (25-33 months old) Sprague-Dawley rats with the use of ex vivo extracellular recordings techniques. In aged rats, the amplitude of the NMDAr-mediated field excitatory postsynaptic potentials (fEPSPs) was not altered, whereas their duration was significantly increased. In contrast, the magnitude of non-NMDAr-mediated fEPSPs was significantly smaller. The presynaptic fiber volley was not affected by age. Considering that the depression of non-NMDAr-mediated responses was previously attributed to fewer synaptic contacts between glutamatergic afferent fibers and pyramidal cells in aged animals (see Barnes et al., Hippocampus 1992;2:457-468), the absence of age-related changes in the amplitude of NMDAr-mediated fEPSPs suggests that compensatory mechanisms may occur. The contribution of gamma-aminobutyric acid (GABA) and acetylcholine to these mechanisms was addressed. The NMDAr-mediated fEPSPs were then recorded (1) in young and aged rats before and after blockade of the GABA(B) receptor-mediated inhibition by the specific antagonist CGP 55845 and (2) in young rats after a selective cholinergic denervation of the hippocampus by the immunotoxin 192 IgG-saporin. The results did not indicate statistically relevant age-related effects of CGP 55845. In contrast, the loss of the cholinergic innervation by the immunotoxin induced a significant increase in both the amplitude and duration of the NMDAr-mediated fEPSPs. Our results indicate that the functional properties of the ionotropic glutamate receptor subtypes located on CA1 pyramidal cells are differentially affected by aging and suggest that the cholinergic deficit that occurs during aging may be involved in the maintenance of robust NMDAr-mediated synaptic responses.
Asunto(s)
Acetilcolina/fisiología , Envejecimiento/fisiología , Hipocampo/fisiología , Ácidos Fosfínicos/farmacología , Terminales Presinápticos/fisiología , Propanolaminas/farmacología , Receptores de N-Metil-D-Aspartato/fisiología , Sinapsis/fisiología , Ácido gamma-Aminobutírico/fisiología , Animales , Anticuerpos Monoclonales/toxicidad , Colinérgicos/toxicidad , Desnervación , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Antagonistas de Receptores de GABA-B , Hipocampo/efectos de los fármacos , Hipocampo/crecimiento & desarrollo , Inmunotoxinas/toxicidad , Masculino , N-Glicosil Hidrolasas , Fibras Nerviosas/efectos de los fármacos , Fibras Nerviosas/fisiología , Terminales Presinápticos/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Proteínas Inactivadoras de Ribosomas Tipo 1 , Saporinas , Sinapsis/efectos de los fármacosRESUMEN
Deficits in cholinergic function have been documented in a variety of brain disorders including Alzheimer's Disease and, to a lesser extent, in normal ageing. In the present article, we have reviewed our recent findings on the effects of the loss of basal forebrain cholinergic neurons on the functional properties of the septohippocampal pathway. In vivo and ex vivo investigations were performed in rats following basal forebrain cholinergic lesion with the specific immunotoxin 192 IgG-saporin. Our results suggest a significant contribution of cholinergic neurons in the rhythmically bursting activity recorded within the medial septum. In addition, they give evidence that acetylcholine may tonically decrease the glutamatergic synaptic responses in the hippocampus whereas the GABAergic mediated inhibitory potentials are not affected. The possible contribution of these cholinergic mechanisms in the age-related functional alterations of the septohippocampal activity is discussed.
Asunto(s)
Colinérgicos/toxicidad , Hipocampo/fisiología , Neuronas/fisiología , Prosencéfalo/fisiología , Tabique Pelúcido/fisiología , Animales , Anticuerpos Monoclonales/toxicidad , Hipocampo/citología , Inmunohistoquímica , Inmunotoxinas/toxicidad , N-Glicosil Hidrolasas , Vías Nerviosas/fisiología , Prosencéfalo/citología , Ratas , Proteínas Inactivadoras de Ribosomas Tipo 1 , Saporinas , Tabique Pelúcido/citologíaRESUMEN
The effects of aging on activation of N-methyl-D-aspartate (NMDA) receptors were studied in the CA1 field of hippocampal slices from young (2-4 months old) and aged (25-32 months old) Sprague-Dawley rats with the use of ex vivo extra- and intracellular electrophysiological recording techniques. No significant age-related changes of the unitary NMDA-receptor mediated excitatory postsynaptic potentials (EPSPs), recorded from the pyramidal cells after stimulation of the stratum radiatum in a magnesium-free medium and isolated in the presence of the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione, were found. Simultaneously, the magnitude of synaptic plasticity which involved NMDA receptor activation was not altered. No significant age-related modifications in the mechanisms controlling glutamate release and of postsynaptic NMDA receptor responsiveness were revealed. Considering the 30-40% decrease in NMDA binding sites in the aged hippocampus, our results suggest the occurrence of compensatory mechanisms which are discussed.
Asunto(s)
Envejecimiento/fisiología , Hipocampo/fisiología , Receptores de N-Metil-D-Aspartato/fisiología , Animales , Estimulación Eléctrica , Potenciales Evocados/efectos de los fármacos , Potenciales Evocados/fisiología , Agonistas de Aminoácidos Excitadores/farmacología , Hipocampo/citología , Hipocampo/metabolismo , Técnicas In Vitro , Potenciación a Largo Plazo/efectos de los fármacos , Masculino , N-Metilaspartato/farmacología , Plasticidad Neuronal/fisiología , Células Piramidales/metabolismo , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/agonistas , Sinapsis/fisiologíaRESUMEN
A complete and selective destruction of the basal forebrain cholinergic neurons projecting to the cerebral cortex and the hippocampus was induced in the rat by the toxin 192 IgG-saporin. Using electrophysiologic techniques, we have investigated the consequences of this cholinergic denervation on inhibitory and excitatory synaptic responses of CA1 pyramidal cells in rat hippocampal slices ex vivo. Histochemical experiments were performed in slices from control and 192 IgG-saporin-treated rats to check the efficacy of the intracerebroventricular injection of the immunotoxin. Stimulation of stratum radiatum elicits a glutamatergic excitatory postsynaptic potentials followed by a biphasic GABAergic inhibitory postsynaptic potential (IPSP). No significant change in IPSP was observed in 192 IgG-saporin-treated rats. By contrast, the N-methyl-D-aspartate (NMDA) and to a lesser extent the non-NMDA components of the glutamatergic response were potentiated in these animals. The possible pre- and postsynaptic mechanisms of this potentiation were discussed.
Asunto(s)
Anticuerpos Monoclonales , Colinérgicos , Ácido Glutámico/fisiología , Hipocampo/fisiología , Inmunotoxinas , Neuronas/fisiología , Parasimpatectomía , Sinapsis/fisiología , Animales , Anticuerpos Monoclonales/administración & dosificación , Colinérgicos/administración & dosificación , Electrofisiología , Potenciales Evocados/fisiología , Hipocampo/citología , Histocitoquímica , Inmunotoxinas/administración & dosificación , Inyecciones Intraventriculares , Masculino , Potenciales de la Membrana/fisiología , N-Glicosil Hidrolasas , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/efectos de los fármacos , Proteínas Inactivadoras de Ribosomas Tipo 1 , SaporinasRESUMEN
The role of corticosteroids in brain aging remains a controversial issue. Conceivably, if corticosteroids levels are increased in the aged brain, neuronal function might be altered. For instance, GABA-mediated synaptic events, spike accommodation and afterhyperpolarizing potentials (AHPs) might be modified. Our electrophysiological results show that the most consistent alterations observed in the aged rat hippocampus concern cholinergic receptors, glutamatergic NMDA receptors and GABAB receptors mediated synaptic potentials. In contrast no consistent alterations were observed in afterhyperpolarizing potentials, calcium spikes, or GABAA mediated synaptic events. Therefore our electrophysiological results are difficult to reconcile with a 'glucocorticoid cascade hypothesis,' involving an elevated level of corticosterone in the aged rat.
Asunto(s)
Corticoesteroides/farmacología , Hipocampo/crecimiento & desarrollo , Sinapsis/fisiología , Animales , Electrofisiología , Hipocampo/efectos de los fármacos , Técnicas In Vitro , Masculino , Ratas , Ratas Endogámicas F344 , Ratas Sprague-Dawley , Ratas Wistar , Receptores de GABA-B/efectos de los fármacos , Receptores de GABA-B/metabolismo , Sinapsis/efectos de los fármacos , Membranas Sinápticas/efectos de los fármacos , Membranas Sinápticas/metabolismo , Ácido gamma-Aminobutírico/metabolismoRESUMEN
The effects of aging on activation of metabotropic glutamate (mGlu) receptors were studied in the CA1 field of hippocampal slices from young (3- to 4-month-old) and aged (24- to 27-month-old) Sprague-Dawley rats with the use of ex vivo electrophysiological recording techniques. The depolarization of membrane potential, the increase in input resistance, and the blockade of the afterhyperpolarization induced in pyramidal cells of young rats by bath application of the mGlu receptor agonist (+/-)-trans-1-aminocyclopentate-1,3-dicarboxylic acid were not altered in aged animals. No age-related changes of the depressive effects of the mGlu receptor agonist were found on either the excitatory glutamatergic postsynaptic potential or the GABA-mediated inhibitory postsynaptic potentials induced by the stimulation of the stratum radiatum. The magnitude of synaptic plasticity involving mGlu receptor activation, although weaker, was not significantly altered in aged rats. This absence of age-related effects on activation of mGlu receptors may be important in understanding the possible origins of the alterations in neuronal plasticity which occur in brain aging.
Asunto(s)
Envejecimiento/metabolismo , Hipocampo/crecimiento & desarrollo , Hipocampo/metabolismo , Receptores de Glutamato Metabotrópico/metabolismo , 2-Amino-5-fosfonovalerato/farmacología , Animales , Benzoatos/farmacología , Cicloleucina/análogos & derivados , Cicloleucina/farmacología , Agonistas de Aminoácidos Excitadores/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Glicina/análogos & derivados , Glicina/farmacología , Hipocampo/citología , Técnicas In Vitro , Masculino , Potenciales de la Membrana/efectos de los fármacos , Plasticidad Neuronal/efectos de los fármacos , Células Piramidales/metabolismo , Ratas , Ratas Sprague-Dawley , Receptores de Glutamato Metabotrópico/efectos de los fármacos , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Sinapsis/efectos de los fármacos , Sinapsis/metabolismoRESUMEN
The influx of calcium into the postsynaptic neuron is likely to be an important event in memory formation. Among the mechanisms that nerve cells may use to alter the time course or size of a spike of intracellular calcium are cytosolic calcium binding or "buffering" proteins. To consider the role in memory formation of one of these proteins, calbindin D28K, which is abundant in many neurons, including the CA1 pyramidal cells of the hippocampus, transgenic mice deficient in calbindin D28K have been created. These mice show selective impairments in spatial learning paradigms and fail to maintain long-term potentiation. These results suggest a role for calbindin D28K protein in temporally extending a neuronal calcium signal, allowing the activation of calcium-dependent intracellular signaling pathways underlying memory function.
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Hipocampo/fisiología , Potenciación a Largo Plazo , Aprendizaje por Laberinto , Memoria/fisiología , Proteínas del Tejido Nervioso/biosíntesis , Neuronas/fisiología , Proteína G de Unión al Calcio S100/biosíntesis , Animales , Calbindina 1 , Calbindinas , ADN sin Sentido , ADN Complementario , Discriminación en Psicología , Estimulación Eléctrica , Técnicas In Vitro , Trastornos de la Memoria/genética , Trastornos de la Memoria/fisiopatología , Ratones , Ratones Transgénicos , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/fisiología , Proteína G de Unión al Calcio S100/genética , Proteína G de Unión al Calcio S100/fisiologíaRESUMEN
The possible role of endogenous cholinergic innervation in hippocampal plasticity is controversial. We studied the role of acetylcholine (ACh) in short- and long-term potentiation (STP and LTP), using the cholinergic neurotoxin 192 IgG-saporin. It was still possible to induce STP the LTP in the CA1 field following complete and selective cholinergic denervation of the hippocampus. This study therefore demonstrates that integrity of the endogenous cholinergic system is not necessary for the induction or maintenance of LTP in the CA1 field of the hippocampus. The consequences in terms of relationship between hippocampal cholinergic system, LTP and memory are discussed.
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Acetilcolina/fisiología , Anticuerpos Monoclonales/farmacología , Colinérgicos/farmacología , Hipocampo/fisiología , Inmunotoxinas/farmacología , Potenciación a Largo Plazo , Análisis de Varianza , Animales , Desnervación , Histocitoquímica , Masculino , N-Glicosil Hidrolasas , Ratas , Ratas Sprague-Dawley , Proteínas Inactivadoras de Ribosomas Tipo 1 , Saporinas , Factores de TiempoRESUMEN
The effects of the metabotropic glutamate (mGlu) receptor agonists (+/-)-trans-1-aminocyclopentane-1,3-dicarboxylic acid (trans-ACPD) or 1S,3R-ACPD on gamma-aminobutyric acid (GABA)-mediated inhibitory synaptic responses have been investigated in vitro in CA1 pyramidal cells of rat hippocampal slices. Bath application of both agonists depolarized the resting membrane potential and increased membrane resistance. Simultaneously, the afterhyperpolarization induced by a burst of spikes as well as spike accomodation were blocked. Stimulation of the stratum radiatum induced in CA1 pyramidal cells an early excitatory postsynaptic potential (EPSP) followed by a fast GABAA and a slow GABAB-mediated inhibitory postsynaptic potentials (IPSPs). All synaptic responses were dose dependently depressed by mGlu receptor agonists. At low concentration, (+/-)-trans-ACPD (10-100 microM) and 1S,3R-ACPD (10 microM) consistently reduced the EPSP, slightly depressed the fast IPSP but greatly decreased the slow IPSP. Increasing the concentration of mGlu receptor agonists to 200 microM and 50 microM, respectively further depressed the EPSP and dramatically reduced the amplitude of both IPSPs. In the presence of the glutamate receptor antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (10 microM) and D-(-)-2-amino-5-phosphonovaleric acid (30 microM), monosynaptically evoked IPSPs were still depressed by mGlu receptor agonists. In the same conditions, the discharge frequency of spontaneous IPSPs which reflect the activity of GABAergic interneurons was enhanced by low doses of mGlu receptor agonists but depressed with higher concentrations. On the other hand, the postsynaptic hyperpolarization and decrease in membrane resistance induced by the GABAB receptor agonist baclofen applied in the bath or by microiontophoresis were not affected by mGlu receptor agonists.(ABSTRACT TRUNCATED AT 250 WORDS)
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Cicloleucina/análogos & derivados , Hipocampo/efectos de los fármacos , Neurotoxinas/farmacología , Terminales Presinápticos/efectos de los fármacos , Receptores de Glutamato Metabotrópico/efectos de los fármacos , Animales , Cicloleucina/farmacología , Masculino , Potenciales de la Membrana/efectos de los fármacos , Células Piramidales/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Factores de Tiempo , Ácido gamma-Aminobutírico/metabolismoRESUMEN
The consequences of intracerebroventricular injection of the toxin 192-IgG-saporin on the electrophysiological properties of CA1 pyramidal cells were investigated using intracellular recordings in the in vitro hippocampal slice preparation. We present the first electrophysiological evidence of a dysfunction of hippocampal cholinergic afferents following injection of 192-IgG-saporin. The synaptic events mediated by acetylcholine were altered in such animals: the slow cholinergic excitatory postsynaptic potentials as well as the cholinergic activation of GABAergic interneurones were dramatically depressed or even absent; the amplitude and duration of the afterhyperpolarization following a burst of spikes were increased, while other neuronal properties were not modified. These specific alterations suggest that the toxin 192-IgG-saporin is a specific tool for the experimental study of cholinergic denervation in the hippocampus.