[Differential diagnosis model of benign and malignant breast BI-RADS category 4 nodules based on serum SP70 and conventional laboratory indicators].

Objective: To develop a nomogram model for the differential diagnosis of benign and malignant breast BI-RADS (Breast Imaging Reporting and Data System) category 4 nodules based on serum tumor specific protein 70 (SP70) and conventional laboratory indicators and validate its predictive efficacy. Methods: A case-control study design was used to retrospectively analyze the data of 429 female patients diagnosed with BI-RADS category 4 breast nodules by breast color doppler flow imaging at the First Affiliated Hospital of Nanjing Medical University from January 2021 to April 2022 with an age range of 16 to 91 years and a median age of 50 years, and the patients were divided into a training cohort (314 patients) and a validation cohort (115 patients) according to the inclusion time successively. Using postoperative pathological findings as the"gold standard", univariate and multivariate logistic regression analyses were used to identify the predictor variables used for the model. The nomogram, receiver operating characteristic (ROC) curves and calibration curves were drawn for the prediction model, and the discrimination and calibration of the model were evaluated using the consistency index (C-index) and calibration plots. Results: The postoperative pathological results showed that 286 (66.7%) were malignant nodules and 143 (33.3%) were benign nodules of 429 breast BI-RADS category 4 nodules. The serum SP70 (OR=1.227,95%CI: 1.033-1.458,P=0.020), NLR (OR=1.545,95%CI: 1.047-2.280,P=0.028), LDL-C (OR=2.215, 95%CI: 1.354-3.622, P=0.002), GLU (OR=2.050,95%CI:1.222-3.438,P=0.007), PT (OR=1.383,95%CI: 1.046-1.828,P=0.023), nodule diameter (OR=1.042, 95%CI: 1.008-1.076, P=0.015) and age (OR=1.062,95%CI: 1.011-1.116,P=0.016) were independent risk factors which could be used to distinguish benign and malignant breast BI-RADS category 4 nodules (P<0.05). The nomogram was plotted by the above seven independent variables, and the concordance index (C-index) for the training cohort and validation cohort were 0.842 (95%CI:0.786-0.898) and 0.787 (95%CI:0.687-0.886), respectively. The sensitivity and specificity of using this model to identify benign and malignant breast BI-RADS category 4 nodules in the training and validation cohort were 83.5%, 72.5% and 79.2%, 73.6%, respectively. The calibration curves showed good agreement between the predicted and actual values in the nomogram. Conclusions: This study combined serum SP70, conventional laboratory indicators and breast color doppler flow imaging to develop a nomogram model for the differential diagnosis of benign and malignant breast BI-RADS category 4 nodules. The model may have good predictive efficacy and may provide a basis for clinical treatment options, which is beneficial for guiding breast cancer screening and prevention.

Hsa-miR-4730 as a new and potential diagnostic and prognostic indicators for pancreatic cancer.

OBJECTIVE: Pancreatic cancer is a gastrointestinal tumor with the highest malignancy and few diagnostic and prognostic markers. Patients with disease have a 5-year survival rate that is not more than 10%. As a research hotspot in recent years, miRs (microRNAs) are differentially expressed in various tumors, so they can be used as the potential diagnostic and prognostic markers. In this study, differentially expressed miRs in patients with pancreatic cancer were screened out through the GEO chip, to provide potential markers for clinical practice. This study aimed to explore the expression and potential value of miR-4730 in pancreatic cancer. PATIENTS AND METHODS: Differentially expressed miRs in pancreatic cancer were analyzed through logging in GEO DataSets to download GSE112264. Fifty patients with pancreatic cancer who were treated in our hospital from May 2012 to January 2014 (Group A), 50 patients with benign pancreatic lesions during the same period (Group B), and 50 healthy individuals undergoing physical examinations (Group C) were enrolled in this study. The expression of miR-4730 in the serum and the cancer tissue was detected by qRT-PCR. The correlation of miR-4730 with pathological data, and the diagnostic values of differential indicators in the data were analyzed. The patients were followed up for 5 years to observe the relationship between miR-4730 and their survival. RESULTS: The analysis of the GEO chip showed 305 differentially expressed miRs, among which 225 were highly expressed and 80 were lowly expressed, with miR-4730 differentially expressed most. The expression of serum miR-4730 in Group A was significantly lower than that in Groups B and C (p<0.05), so miR-4730 had a diagnostic value. The expression of miR-4730 in the cancer tissue was significantly lower than that in the adjacent tissue. The correlation analysis showed that the expression of miR-4730 in the cancer tissue was positively correlated with that in the serum. Patients with low miR-4730 expression had poorly differentiated pancreatic cancer, and patients with stages III+IV of pancreatic cancer had higher incidences of lymphatic invasion and distal metastasis (p<0.05), so miR-4730 had a diagnostic value. The 3- and 5-year survival rates in the high miR-4730 expression group were higher than those in the low expression group (both p<0.05). TNM staging, lymphatic invasion, distal metastasis, and miR-4730 were independent prognostic factors for the 3- and 5-year survival of patients with pancreatic cancer. CONCLUSIONS: For patients with pancreatic cancer, those with low miR-4730 expression have poor survival and prognoses, so miR-4730 can be used as a potential observational index for the prognosis and diagnosis of the disease.

[Functional Characterization of ZmHAK1 Promoter and Its Regulatory Transcription Factors in Maize].

Potassium (K^(+)) deficiency in the soil may seriously affect the yield and quality of plants, which usually satisfy their potassium requirements by engaging their K^(+) transporters and/or channels. High-affinity potassium transporter (ZmHAK) family members play crucial role in the uptake and distribution of K^(+) in maize (Zea mays L.). Here, we describe the function of ZmHAK1 promoter and its upstream regulatory transcription factors in maize. In this plant, HAK gene family includes 34 protein-encoding members, with their phylogenetic tree analysis showing both evolutionary conservativeness and diversity. ZmHAK1 gene promoter contains many functional elements related to abiotic stress. Reporter construct pCambia1301:ProZmHAK1:GUS shows that the ZmHAK1 gene is active in the roots, stems, and leaves. Using yeast one-hybrid experiment, we showed that the ZmHAK1 promoter interacts with the transcription factors ZmRAP2.11 and ZmARF2, and that these interactions occur on different fragments of the ZmHAK1 promoter. Transcription factor ZmRAP2.11 localizes in the nucleus, while ZmARF2 is found both in the nucleus and in the cell cytoplasm. In conclusion, our results suggest that the ZmHAK1 regulation has an important role in the process of absorbing potassium ions, and possibly in the response of maize to abiotic stress.

Circular RNA-ABCB10 suppresses hepatocellular carcinoma progression through upregulating NRP1/ABL2 via sponging miR-340-5p/miR-452-5p.

OBJECTIVE: Hepatocellular carcinoma (HCC) is one of the most common liver malignancies worldwide with a high rate of recurrence and mortality. Circular RNA-ABCB10 (circ-ABCB10), 724 nucleotides in length, plays a pro-oncogenic role in tumor progression. However, the role of circ-ABCB10 in HCC is still unknown. Therefore, the objective of this study was to determine the role of circ-ABCB10 in HCC progression in vitro and in vivo and to elucidate the underlying mechanism. PATIENTS AND METHODS: Tumor tissues from patients with HCC and multiple HCC cell lines were used for in vitro experiments and a mouse xenograft model was used for in vivo experiments. Quantitative Real Time-PCR, Western blots, lentivirus transfection, cell proliferation assays, cloning formation, migration, and invasion assays, flow cytometry, Luciferase reporter assays, and biotin-coupled probe pull-down assays were performed to investigate the mechanism underlying the effect of circ-ABCB10 on HCC. RESULTS: The results revealed that the expression of circ-ABCB10 was downregulated in both HCC tissues and cell lines and was positively correlated with histological grade and tumor size. The overexpression of circ-ABCB10 exerted inhibitory effects on HCC cell proliferation, invasion, and migration. Mechanistic and functional evidence together showed that circ-ABCB10 elevated expressions of neuropilin-1 (NRP1) and ABL related gene (ABL2) by sponging miR-340-5p and miR-452-5p, which inhibited the progression of HCC. Furthermore, the in vivo study suggests that circ-ABCB10 inhibited tumor growth in nude mice. CONCLUSIONS: In brief, the results demonstrate that circ-ABCB10 exerts anti-tumor roles via miR-340-5p/miR-452-5p-NRP1/ABL2 signaling axis, providing a promising biomarker and therapeutic target for HCC.

Half-dose ganciclovir preemptive treatment of cytomegalovirus infection after pediatric allogeneic hematopoietic stem cell transplantation.

BACKGROUND: Ganciclovir (GCV) has been widely used as preemptive therapy after hematopoietic stem cell transplantation (HSCT), although bone marrow suppression is a known accompaniment, with secondary infection or bleeding as potential complications. Our aim was to evaluate clinical outcomes in pediatric patients with low cytomegalovirus (CMV) antigenemia levels using half the dosage of GCV generally given preemptively. METHODS: Patients received half doses of intravenous GCV (5 mg/kg once daily, 6 days/week) at CMV antigenemia levels <10/200,000 cells. At higher levels of CMV antigenemia, conventional doses of GCV (5 mg/kg every 12 h) were administered. RESULTS: A total of 130 patients were evaluated, detecting CMV antigenemia in 87 (66.9%). Of these patients, 74 (85.1%) were treated preemptively with half-dose GCV, which proved effective as sole therapy in 51 (68.9%). CMV retinitis developed in 4 patients, 2 of whom initially were given half-dose GCV. All infections resolved successfully, with no CMV-related deaths. CMV seropositivity in recipients was the only significant risk factor for positive CMV antigenemia (hazard ratio [HR] = 10.05, P = 0.046). Compared with half-dose GCV administration, conventional GCV dosing resulted in a higher rate of severe neutropenia, defined as absolute neutrophil count <0.5 × 10(9) /L (HR = 4.30, P = 0.015). CONCLUSION: Half-dose GCV therapy at CMV antigenemia levels <10/200,000 cells is an effective and safe means of preemptively treating pediatric CMV infection after HSCT.

Influence of ionic thiocyanate on growth of cabbage, bean, and tobacco.

Plant weight and contents of chlorophyll, ionic thiocyanate (SCN(-)), and hydrogen cyanide (HCN) were determined in cabbage (Brassica oleracea var.capitula L. cv. Early Greenball), bean (Phaseolus vulgaris L. cv. Contender), and tobacco (Nicotiana tabacum L. cv. Delhi 76) grown hydroponically in modified Hoagland's nutrient solution with six concentrations of SCN(-) (supplied as KSCN) (0, 5, 25, 50, 100, and 200 mg/liter). Whereas tobacco plants did not grow with any level of SCN(-) in the culture solution, beans grew with 5 mg/liter and cabbages grew with between 5 and 50 mg/ liter. Increasing levels of SCN(-) in the culture solution resulted in decreased growth and chlorophyll content, accompanied by consistently increasing amounts of SCN(-) in cabbage. Small amounts of HCN found only in tissues of cabbage were not influenced by levels of SCN(-). The greater insensitivity of cabbages to the presence of SCN(-) compared with beans is apparently related to the presence of endogenous glucosinolates which are capable of being degraded into SCN(-). Accumulation of SCN(-) and occurrence of leaf chlorosis in cabbage and beans and death of tobacco plants supplied with SCN(-) in hydroponic culture confirm the capacity of SCN(-) as an allelopathic agent, but its effect mechanism in ecology needs to be demonstrated.