RESUMEN
In the present study, comparative proteomic analysis was performed in rats subjected to water immersionrestraint stress (WRS). A total of 26 proteins were differentially expressed and identified using matrixassisted laser desorption/ionization time of flight mass spectrometry. Among the 26 differentially expressed protein spots identified, 13 proteins were significantly upregulated under WRS, including pyruvate kinase and calreticulin, which may be closely associated with energy metabolism. In addition, 12 proteins were downregulated under WRS, including hemoglobin subunit ß2 and keratin type II cytoskeletal 8, which may be important in protein metabolism and cell death. Gene Ontology analysis revealed the cellular distribution, molecular function and biological processes of the identified proteins. The mRNA levels of certain differentially expressed proteins were analyzed using fluorescence quantitative polymerase chain reaction analysis. The results of the present study aimed to offer insights into proteins, which are differentially expressed in gastric ulcers in stress, and provide theoretical evidence of a radical cure for gastric ulcers in humans.
Asunto(s)
Proteoma , Proteómica , Restricción Física , Úlcera Gástrica/etiología , Úlcera Gástrica/metabolismo , Estrés Fisiológico , Animales , Biología Computacional/métodos , Bases de Datos Genéticas , Modelos Animales de Enfermedad , Electroforesis en Gel Bidimensional , Mucosa Gástrica/metabolismo , Mucosa Gástrica/patología , Ontología de Genes , Masculino , Anotación de Secuencia Molecular , Proteómica/métodos , Ratas , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Úlcera Gástrica/patologíaRESUMEN
OBJECTIVE: To review the research progress on Type IV secretion system (T4SS) in Helicobacter pylori. DATA SOURCES: The data used in this review were identified by searching of PUBMED (1995 - 2007) online resources using the key terms 'Type IV secretion system' and 'Helicobacter pylori'. STUDY SELECTION: Mainly original articles and critical reviews written by major pioneer investigators of this field were selected. RESULTS: The research progress on T4SS in Helicobacter pylori was summarized. The structure and function was discussed. CONCLUSIONS: T4SS is not only involved in toxin secretion and injection of virulence factors into eukaryotic host target cells, but also involved in horizontal DNA transfer to other bacteria and eukaryotic cells, through DNA uptake from or release into the extracellular milieu. It provides a new insight into the pathogenicity of Helicobacter pylori and a novel target for antimicrobials development. However, many challenges remain for us in understanding the biological role of T4SS in Helicobacter pylori.
