RESUMEN
Pure rabbit fibrinogen was prepared by a method involving two ammonium sulfate precipitations, one 2 M phosphate buffer precipitation, one DEAE cellulose chromatography and lastly one Sepharose 6 B chromatography. The aminoacid composition was determined and an immunonephelemetric assay was proposed. This assay followed an accurate determination of fibrinogen concentration in a rabbit with inflammatory reaction.
Asunto(s)
Fibrinógeno , Aminoácidos/análisis , Animales , Cromatografía DEAE-Celulosa , Cromatografía en Gel , Fibrinógeno/aislamiento & purificación , Inmunoelectroforesis , Masculino , ConejosRESUMEN
An immunonephelemetric determination of rabbit haptoglobin was carried out. At first, pure haptoglobin was isolated and then monospecific immunserums were obtained from rats. This method completes the regular determination of haptoglobin in all cases where the peroxydasic activity of the haptoglobin-hemoglobin complex is inhibited either by an hemolytic process, as is the case for rabbits, or by some inhibitors of the peroxydasic reaction.
Asunto(s)
Haptoglobinas/análisis , Inmunoensayo/métodos , Animales , Autoanálisis , Compuestos Bicíclicos con Puentes/farmacología , Precipitación Química , Cromatografía por Intercambio Iónico , Cicloheptanos/farmacología , Haptoglobinas/inmunología , Haptoglobinas/aislamiento & purificación , Inflamación/inducido químicamente , Inflamación/metabolismo , Masculino , Nefelometría y Turbidimetría , Conejos , Ratas/inmunología , Factores de TiempoRESUMEN
In 100 patients suffering from intravascular haemolysis, a "protein profile" was made on the basis of measurement of haptoglobin, haemopexin and orosomucoid. The estimations were carried out using a rapid precise automatic technique using monospecific immune serum. The results obtained indicate that these tests are sensitive and reliable.