Visualization and temporal/spatial characterization of cardiac radiofrequency ablation lesions using magnetic resonance imaging.

BACKGROUND: The purpose of this study was to describe a system and method for creating, visualizing, and monitoring cardiac radiofrequency ablation (RFA) therapy during magnetic resonance imaging (MRI). METHODS AND RESULTS: RFA was performed in the right ventricular apex of 6 healthy mongrel dogs with a custom 7F nonmagnetic ablation catheter (4-mm electrode) in a newly developed real-time interactive cardiac MRI system. Catheters were positioned to intracardiac targets by use of an MRI fluoroscopy sequence, and ablated tissue was imaged with T2-weighted fast spin-echo and contrast-enhanced T1-weighted gradient-echo sequences. Lesion size by MRI was determined and compared with measurements at gross and histopathological examination. Ablated areas of myocardium appeared as hyperintense regions directly adjacent to the catheter tip and could be detected 2 minutes after RF delivery. Lesions reached maximum size approximately 5 minutes after ablation, whereas lesion signal intensity increased linearly with time but then reached a plateau at 12.2+/-2.1 minutes. Lesion size by MR correlated well with actual postmortem lesion size and histological necrosis area (55.4+/-7.2 versus 49.7+/-5.9 mm(2), r=0.958, P<0.05). CONCLUSIONS: RFA can be performed in vivo in a new real-time interactive cardiac MRI system. The spatial and temporal extent of cardiac lesions can be visualized and monitored by T2- and T1-weighted imaging, and MRI lesion size agrees well with actual postmortem lesion size. MRI-guided RFA may be a useful approach to help facilitate anatomic lesion placement and to provide insight into the biophysical effects of new ablation techniques and technologies.

Prospective comparison of lesions created using a multipolar microcatheter ablation system with those created using a pullback approach with standard radiofrequency ablation in the canine atrium.

The aim of this study was to compare the lesions created using a multipolar microcatheter (MICRO) ablation system in the right canine atrium to a pullback approach with a standard radiofrequency (STND RF) ablation and to determine the value of electrogram amplitude and pacing threshold in predicting transmurality of lesions. Ten dogs underwent right atrial ablation using a MICRO (6 dogs) or STND RF (4 dogs) ablation system in each animal. Attempts were made to create linear RF lesions at four predetermined atrial sites. RF energy was delivered for 60 seconds using closed-loop, temperature control to achieve a target temperature of 60 degrees C for STND RF and 50 degrees C for MICRO. Unipolar atrial electrogram amplitude and atrial pacing threshold were obtained before and after ablation. Pathological analysis was determined at 4 weeks after ablation. Lesions created with MICRO were narrower, more likely to be continuous, and more likely to be anchored to an anatomic structure than those lesions which were created using a STND RF. No difference was observed in overall lesion length or in the proportion of lesions that were transmural over at least 50% of their length. Of lesions created using MICRO, a significant relation was observed between transmurality of lesion and unipolar electrogram amplitude as well as pacing threshold. Further studies are needed to determine if this type of ablation technique and parameters during ablation may facilitate a successful catheter-based MAZE procedure.

Relation between impedance and electrode temperature during radiofrequency catheter ablation of accessory pathways and atrioventricular nodal reentrant tachycardia.

OBJECTIVES: Impedance monitoring has been proposed as a method to assess the adequacy of tissue heating during catheter ablation procedures. The purpose of this study was to evaluate the relation among initial impedance, fall in impedance, and electrode temperature during catheter ablation procedures. METHODS AND RESULTS: Data from 248 applications of radiofrequency energy in 45 consecutive patients (26 with accessory pathways and 19 with atrioventricular nodal reentrant tachycardia) referred for catheter ablation were analyzed. The initial impedance was higher during ablation of accessory pathways than during atrioventricular nodal reentrant tachycardia (116+/-66 versus 106+/-80 omega, P < .001). In both groups, a significant correlation was observed between the initial impedance and temperature (R = 0.98, P < .001). After accounting for differences between patients and ablation targets, an even closer correlation was observed (accessory pathways: R = 0.95, P < .0001; atrioventricular nodal reentrant tachycardia: R = 0.94, P < .0001). CONCLUSION: These data suggest that monitoring of the initial impedance and the fall in impedance during ablation procedures may provide clinically valuable information to assess the efficacy of tissue heating and lesion formation.

Interaction between neuronal nitric oxide synthase and inhibitory G protein activity in heart rate regulation in conscious mice.

Nitric oxide (NO) synthesized within mammalian sinoatrial cells has been shown to participate in cholinergic control of heart rate (HR). However, it is not known whether NO synthesized within neurons plays a role in HR regulation. HR dynamics were measured in 24 wild-type (WT) mice and 24 mice in which the gene for neuronal NO synthase (nNOS) was absent (nNOS-/- mice). Mean HR and HR variability were compared in subsets of these animals at baseline, after parasympathetic blockade with atropine (0.5 mg/kg i.p.), after beta-adrenergic blockade with propranolol (1 mg/kg i.p.), and after combined autonomic blockade. Other animals underwent pressor challenge with phenylephrine (3 mg/kg i.p.) after beta-adrenergic blockade to test for a baroreflex-mediated cardioinhibitory response. The latter experiments were then repeated after inactivation of inhibitory G proteins with pertussis toxin (PTX) (30 microgram/kg i.p.). At baseline, nNOS-/- mice had higher mean HR (711+/-8 vs. 650+/-8 bpm, P = 0.0004) and lower HR variance (424+/-70 vs. 1,112+/-174 bpm2, P = 0.001) compared with WT mice. In nNOS-/- mice, atropine administration led to a much smaller change in mean HR (-2+/-9 vs. 49+/-5 bpm, P = 0.0008) and in HR variance (64+/-24 vs. -903+/-295 bpm2, P = 0.02) than in WT mice. In contrast, propranolol administration and combined autonomic blockade led to similar changes in mean HR between the two groups. After beta-adrenergic blockade, phenylephrine injection elicited a fall in mean HR and rise in HR variance in WT mice that was partially attenuated after treatment with PTX. The response to pressor challenge in nNOS-/- mice before PTX administration was similar to that in WT mice. However, PTX-treated nNOS-/- mice had a dramatically attenuated response to phenylephrine. These findings suggest that the absence of nNOS activity leads to reduced baseline parasympathetic tone, but does not prevent baroreflex-mediated cardioinhibition unless inhibitory G proteins are also inactivated. Thus, neuronally derived NO and cardiac inhibitory G protein activity serve as parallel pathways to mediate autonomic slowing of heart rate in the mouse.

Prospective comparison of temperature guided microwave and radiofrequency catheter ablation in the swine heart.

Microwave energy has been proposed as an alternative to radiofrequency energy for use during catheter ablation procedures. The purpose of this study was to prospectively compare, in an animal model, the lesion size associated with temperature guided catheter ablation using either microwave or radiofrequency energy. Eleven swine underwent catheter ablation with either radiofrequency (N = 4) or microwave energy (N = 7). In each animal catheter ablation was performed at 7-15 sites. At each site energy was delivered for 60 seconds using closed loop feedback temperature control to achieve a target temperature of 70 degrees C. Cardiac catheterization was performed before and after ablation. Animals were sacrificed approximately one month following the ablation procedure. Analysis of lesion size demonstrated that overall lesions created using radiofrequency energy were larger than those created using microwave energy. In the ventricle, lesions created using microwave energy were longer, but had a similar width and depth as those created using radiofrequency energy. An important relation was observed between the depth of lesions created using microwave energy and catheter stability, as evidenced by the temperature profile. Overall, lesions created using microwave energy are smaller than those created using radiofrequency energy. Catheter stability has an important impact on lesion size.