RESUMEN
A novel alphaproteobacterium was isolated from the well water of a thermal bath at Budapest, Hungary. Phylogenetic analysis of the novel strain showed that this bacterium belongs to a distinct lineage among the genus Brevundimonas. Based on the 16S rRNA gene sequence strain FDRGB2bT showed the highest sequence similarity values to Brevundimonas naejangsanensis BIO-TAS2-2T (97.35â%), Brevundimonas viscosa F3T (97.28â%), Brevundimonas vesicularis LMG 2350T (97.27â%), Brevundimonas nasdae GTC 1043T (97.14â%), Brevundimonas vancanneytii LMG 2337T (97.13â%) and Brevundimonas aurantiaca DSM 4731T (97.13â%). The newly isolated bacterium was strictly aerobic, and its optimum growth occurred at 20-30 °C, between pH 8-9 and without NaCl. Movement was with a single polar flagellum, but the cells could also produce stalks. The major isoprenoid quinone of strain FDRGB2bT was Q-10, the major cellular fatty acids were C18â:â1ω7c and C16â:â0, and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and four unknown glycolipids. The characteristic diamino acid in its cell wall is meso-diaminopimelic acid. The G+C content of DNA of the type strain was 69.8 mol%. Strain FDRGB2bT (=DSM 29841T=NCAIM B.02621T) is proposed as the type strain of a novel species with the proposed name Brevundimonas balnearis sp. nov.
Asunto(s)
Caulobacteraceae/clasificación , Filogenia , Microbiología del Agua , Pozos de Agua , Técnicas de Tipificación Bacteriana , Composición de Base , Caulobacteraceae/genética , Caulobacteraceae/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/química , Hungría , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Temperatura , Ubiquinona/análogos & derivados , Ubiquinona/químicaRESUMEN
Strains of a novel alphaproteobacterium were isolated from ultrapure water of a Hungarian power plant on a newly developed medium. Phylogenetic analysis of the 16S rRNA gene sequences of the novel strains showed that these bacteria belong to a distinct lineage far from any known taxa. Based on the 16S rRNA gene sequences, strains PI_31, PI_25 and PI_21(T) exhibited the highest sequence similarity to Bosea minatitlanensis AMX51(T) (93.43â%) and Bosea thiooxidans DSM 9653(T) (93.36â%); similarity to all other taxa was less than 93.23â%. Fatty acid profiles, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of cell extracts as well as physiological and biochemical characteristics indicated that our strains represent a novel genus and species within the class Alphaproteobacteria. The major isoprenoid quinone of the strains was Q-10, the major cellular fatty acids were C18â:â1ω7c and 11-methyl C18â:â1ω7c and the polar lipid profiles of the strains contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and several unknown phospholipids and other lipids. The characteristic diamino acid in their cell wall was meso-diaminopimelic acid. The G+C content of DNA of the proposed type strain PI_21(T) was 68.9 mol%. A new genus and species, Phreatobacter oligotrophus gen. nov., sp. nov., is proposed to accommodate the strains. Strain PI_21(T) (â=âDSM 25521(T)â=âNCAIM B 02510(T)) is the type strain of Phreatobacter oligotrophus.
Asunto(s)
Alphaproteobacteria/clasificación , Filogenia , Centrales Eléctricas , Microbiología del Agua , Purificación del Agua , Alphaproteobacteria/genética , Alphaproteobacteria/aislamiento & purificación , Técnicas de Tipificación Bacteriana , Composición de Base , Pared Celular/química , ADN Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Grasos/química , Hungría , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
A Gram-positive actinobacterium, strain IV-75(T), was isolated by using R2A agar from the ultrapure water system of a power plant in Hungary. The strain exhibited a rod-coccus cell cycle, and was strictly aerobic, non-motile, catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis revealed that strain IV-75(T) belonged to the suborder Micrococcineae and clustered with members of the family Intrasporangiaceae. Its closest phylogenetic neighbour was Arsenicicoccus bolidensis CCUG 47306(T) (94.3% 16S rRNA gene sequence similarity). The peptidoglycan of strain IV-75(T) contained meso-diaminopimelic acid and MK-10(H(4)) was the major menaquinone. The polar lipid pattern contained phosphatidylglycerol, two unidentified phospholipids, one glycolipid and several other lipid components. The major fatty acids were anteiso-C(15:0), C(18:1)ω9c and C(16:0). Based on the moderate levels of 16S rRNA gene sequence similarity to all members of the family Intrasporangiaceae and the unique combination of chemotaxonomic characteristics, strain IV-75(T) is considered to represent a novel species of a new genus, for which the name Aquipuribacter hungaricus gen. nov., sp. nov. is proposed. The type strain of Aquipuribacter hungaricus is IV-75(T) (=DSM 21674(T)=NCAIM B 02333(T)).
