Human Genetic Markers and Structural Prediction of Plasmodium falciparum Multidrug Resistance Gene (pfmdr1) for Ligand Binding in Pregnant Women Attending General Hospital Minna.

The study aims to determine the association of malaria infection with ABO blood groups and genotype and also to detect point mutations at positions 86, 184, 1034, and 1042 of the Plasmodium falciparum multidrug resistance gene (pfmdr1) in blood samples collected from pregnant women attending General Hospital Minna. Out of 250 pregnant women screened, 39 (15.60%) had malaria infection. Prevalence was higher in women, during the third trimester (46.15%), genotype AA (64.10%), and O blood group (53.84%) individuals when compared with others. There was significant (p < 0.05) decrease in Packed Cell Volume (PCV), hemoglobin (HGB), Red Blood Cells (RBC), and platelet (PLC) count in infected group when compared with noninfected group. Although, two of the isolates showed disrupted protein sequence at codon 1034-1042, no mutation was found in any of the P. falciparum isolates. Structural prediction of chemical ligand led to the identification of Neu5Acα2-3Galß1-3/ß1-4Glc/GlcNAc. This compound can theoretically bind and change the functional integrity of the pfmdr1 protein, thus providing a new window for malaria drug target.

In Vivo Antiplasmodial and Analgesic Effect of Crude Ethanol Extract of Piper guineense Leaf Extract in Albino Mice.

Antiplasmodial and analgesic effects of crude ethanol extract of Piper guineense was investigated in mice. The antiplasmodial and analgesic efficacy of the extract was judged on its ability to reduce parasitemia and writhing, respectively, in mice. The antiplasmodial screening involved treating infected mice with 200, 400, and 600 mg/kg body weight of extract while the positive control group was given standard artesunate drug. The analgesic test was carried out by administering 1000, 1500, and 2000 mg/kg body weight of extract to three groups of healthy mice, respectively, after induction of pain with 0.75% acetic acid. The positive control group was given aspirin drug. Parasitemia was reduced by 28.36%, 43.28%, and 62.69% in a dose-dependent pattern in the curative test which was significantly different (P < 0.05) from 96.03% of the standard drug. The reduction of writhing by mice given the extract was also dose-dependent (36.29, 45.43, and 59.07%). Aspirin drug was however more effective (86.36%). The extract was safe at 2000 mg/kg body weight. Phytochemical screening revealed the presence of flavonoids, tannins, phlobatannins, terpenoids, and coumarins. Result obtained in this study demonstrated the efficacy of ethanol extract of Piper guineense as an antiplasmodial and analgesic agent.

Evaluation of haematological changes in Plasmodium-berghei-infected mice administered with aqueous extract of Phyllantus amarus.

This study was designed to evaluate the changes in some hematological parameters of P-berghei-infected mice treated with aqueous extract of Phyllantus amarus, a plant that is used traditionally to treat malaria patients in some Nigerian communities. The aqueous extract of the leaves at 200, 400 and 600 mg kg(-1) body weight/day dose levels were used to treat the test groups immediately after infection for the suppressive test and 72 hours post infection for the curative test while a standard antimalarial drug, Artesunate, at a dose of 50 mg kg(-1) body weight was administered on the positive control group. The negative control group was left untreated. The level of parasitemia, variation in weight, Percentage Packed Cell Volume (% PCV), erythrocytes (RBC) and leukocytes (WBC) counts in the different groups were monitored throughout the period of study. The crude extract was screened for its phytochemical composition. The crude extract at 200, 400 and 600 mg kg(-1) body weight/day suppressed parasitemia by 54.67, 61.25 and 61.24% after treating for four days in the suppressive test as against 72.32% for the standard drug while the level of parasitemia was reduced by 64.35, 66.71 and 67.13%, respectively after treating for five days in the curative test as against 71.87% for the standard drug. The variations in the values of Percentage Packed Cell Volume (% PCV), weight, leukocyte and erythrocyte counts for treated groups before and after treatment was not significant (p < 0.05). Alkaloids, flavonoids, tannins, glycosides, saponin, carbohydrate and phenols were found to be present in the crude extract. The findings of this study show that the use of Phyllantus amarus as antimalaria regimen by local medical practitioners does not adversely affect the weight and the haematological parameters determined.