Asunto(s)
Bronquiectasia , Bronquiolitis , Bronquitis Crónica , Fibrosis Quística , Antibacterianos/uso terapéutico , Agua Corporal/metabolismo , Bronquiectasia/tratamiento farmacológico , Bronquiectasia/etiología , Bronquiolitis/tratamiento farmacológico , Bronquiolitis/etiología , Bronquitis Crónica/tratamiento farmacológico , Bronquitis Crónica/etiología , Fibrosis Quística/etiología , Fibrosis Quística/microbiología , Humanos , Macrólidos/uso terapéutico , Moco/metabolismo , Infiltración Neutrófila/inmunología , Infecciones por Pseudomonas , Pseudomonas aeruginosa/patogenicidad , Pseudomonas aeruginosa/fisiología , Percepción de Quorum , Sistema Respiratorio/inmunología , Sistema Respiratorio/metabolismo , Sistema Respiratorio/microbiología , Linfocitos T/inmunologíaRESUMEN
Of the non-physiological compounds in glucose-rich peritoneal dialysis fluid, we investigated the synergistic cytotoxicity of acidity and 3,4-Dideoxyglucosone-3-ene(3,4-DGE) under the existence of lactate using human peritoneal mesothelial cells (HPMC). The effect of pH on cell viability at various levels of pH (5.5, 6.7, 7.15), with or without lactate was examined by adding 1N-HCl to phosphate buffer solution. We also examined the cytotoxic effects of 3,4-DGE and pH (5.5, 6.7 or 7.15). Additionally, we compared the cytotoxic effects of 3,4-DGE and pH (5.5, 6.7 or 7.15) under existence of lactate (40 meq/L) or absence of lactate. The cells were exposed to these solutions for 2 or 4 h. Cell viability was determined by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenylterazolium bromide) assay. 3,4-DGE or acidic solution alone had no significant effects on MTT viability under the absence of lactate. However, acidic solutions containing 3,4-DGE significantly decreased MTT viability under the existence of lactate. The MTT viability of HPMC was not decreased by 3,4-DGE or acidity alone under the absence of lactate. However, the combination of acidity and 3,4-DGE markedly decreased MTT viability under the existence of lactate, strongly suggesting the synergistic cytotoxicity of 3,4-DGE and acidity under the existence of lactate.
Asunto(s)
Ácido Láctico/farmacología , Pironas/farmacología , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Soluciones para Diálisis/química , Soluciones para Diálisis/farmacología , Sinergismo Farmacológico , Células Epiteliales/efectos de los fármacos , Glucosa/farmacología , Humanos , Concentración de Iones de Hidrógeno , Diálisis Peritoneal , Peritoneo/citología , Peritoneo/efectos de los fármacos , Factores de TiempoRESUMEN
Of the nonphysiological compounds in glucose-rich peritoneal dialysate, we investigated the cytotoxicity and synergistic cytotoxicity of acidity and glucose degradation products (GDPs) using human peritoneal mesothelial cells (HPMC). The effect of pH on cell viability was examined by adding 1N HCl to a phosphate-buffered solution (pH > or = 5.5). We also examined the cytotoxic effects of various GDPs [glyoxal (GO), methylglyoxal (MGO), and 3-deoxyglucosone (3DG), alone or in combination] and pH (5.5 or 6.7). The cells were exposed to these solutions for 2 or 4 h. Cell viability was determined by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. Although the MTT viability of HPMC was not decreased by GDP or acidity alone, the combination of acidity and GDP markedly decreased MTT viability, strongly suggesting the synergistic cytotoxicity of GDP and acidity.