Asunto(s)
Sistema del Grupo Sanguíneo ABO/inmunología , Transfusión de Componentes Sanguíneos , Donantes de Sangre/clasificación , Isoanticuerpos/inmunología , Adolescente , Adulto , Factores de Edad , Anciano , Tipificación y Pruebas Cruzadas Sanguíneas , Humanos , Isoanticuerpos/sangre , Masculino , Persona de Mediana Edad , Prevalencia , Factores Sexuales , Adulto JovenRESUMEN
Isoenzyme patterns and the polymorphism of lactate dehydrogenase (LDH) were investigated in 3 fish species of family Cyprinidae, i.e. tench (Tinca tinca), crucian carp (Carassius carassius) and carp (Cyprinus carpio). The isoenzyme patterns were tissue and species specific. In crucian carp subunits with different electrophoretic mobility are present, which are genetically controlled from the B1, B2, A1, A2 and C loci, while the set of loci in carp is B1, B2, A, C1 and C2 and in tench B, A, C. The locus B of LDH in tench, the locus B2 in crucian carp, and the loci B1, C1 and C2 in carp are polymorphic and have two different alleles in each case. The polymorphism did not affect the total LDH activity in the tissues. All the populations investigated were in Hardy-Weinberg equilibrium. The genetic control of the polymorphism in B1 and C1 loci in carp was proved by test matings. The polymorphism in B loci tested in erythrocytes may be utilized as genetic markers in the fish breeding.
Asunto(s)
Peces/genética , Isoenzimas/genética , L-Lactato Deshidrogenasa/genética , Polimorfismo Genético , Animales , Carpas/genética , Mapeo Cromosómico , Electroforesis en Gel de Almidón , Isoenzimas/análisis , EspectrofotometríaRESUMEN
Seven transferrin variants (A,B,C,D,E,F, and G) have been found in carp sera (Cyprinus carpio L.). Genetic analysis involves five variants and agrees with the hypothesis of simple codominant autosomal inheritance at one transferrin (Tf) locus in spite of the fact that the carp is a tetraploid in relation to other species of the same family. Carp populations from three regions were studied which differed in gene frequencies. Individual populations were in Hardy-Weinberg equilibrium. The polymorphism of carp transferrins can be used for the identification of offspring of single parent pairs, stocked in one pond. Transferrins have been isolated and characterized. Homozygous phenotypes comprised four iron-binding components differing in electrophoretic mobility. This heterogeneity is not caused by sialic acid, which is absent. Amino acid composition, content of hexoses (1 mole/mole of protein) and hexosamines (1 mole/mole of protein), molecualr weight (70,000), and the isoelectric point (5.0) have been determined. No N-terminal amino acid could be detected.
