Exploring patterns of antibiotics during and after COVID-19 pandemic in wastewaters of northern Greece: Potential adverse effects on aquatic environment.

Antibiotics, recognized as Emerging Contaminants (ECs), have raised concerns due to their pervasive presence in wastewater treatment plants (WWTPs) and subsequent release into aquatic environments, posing potential ecological risks and contributing to the development of antibiotic-resistant genes. The COVID-19 pandemic prompted an unprecedented surge in antibiotic consumption, necessitating a comprehensive assessment of its impact on antibiotic levels in wastewater. In this light, a four-year monitoring study (2020-2023) was conducted in a WWTP located in the Northern Greece (Thessaloniki), employing High-Resolution Mass Spectrometry (HRMS) technology to monitor twenty antibiotics, during distinct phases pre-, during, and post-COVID-19. Our findings revealed that macrolides and fluoroquinolones were among the most often detected categories during the sampling period. Among the compounds detected, azithromycin and clarithromycin showed the most significant increases during the pandemic, doubling their average concentrations. This establishes a clear correlation between the rise in their concentrations and the incidence of COVID-19 cases. A general downward trend after 2021 was attributed to the new restrictions posed in Greece during this year, regarding the liberal prescription of antibiotics. Seasonal variation revealed a minute augmentation of antibiotics' use during the months that infections are increased. Additionally, the study highlights the ecological risks associated with elevated antibiotic presence and emphasizes the need for continued monitoring and regulatory measures to mitigate potential ecological repercussions. These findings contribute to our understanding of the complex interplay between antibiotic consumption, environmental presence, and the COVID-19 pandemic's impact on antibiotic pollution in WWTPs.

Do poly(lactic acid) microplastics instigate a threat? A perception for their dynamic towards environmental pollution and toxicity.

Fears concerning microplastics (MPs) environmental fate and persistence are progressively expanding on a global basis, with the emphasis given to manufacturing bioplastics for substituting petro-derived plastics extensively growing. Among them, poly(lactic acid) (PLA) holds a pioneering role towards the replacement of conventional polymeric materials, owing to its multifunctional properties, enclosing superior mechanical properties, low cost, renewability, great biocompatibility, transparency, and thermoplasticity launching many fields of application. Due to the wide applicability of PLA in several sectors of everyday life, its waste to be released into the environment is expected to follow a growing tendency during the upcoming years. Even though PLA is a biodegradable polyester, it actually degrades under specific composting environments, including a rich oxygen environment with high temperatures (58-80 °C), high humidity (>60% moisture) as well as the presence of micro-organisms (thermophilic bacteria). Additionally, in various studies it has been implied that PLA displays slower degradation performance when found in blends with other conventional polymers, underlining the unspecified effects on PLA degradation profile, keeping thus the information about PLA degradation from a blur standpoint. Therefore, a deepened understanding of the fate and dynamic effects of PLA MPs is of primary importance. Nevertheless, the current examination of the effects of PLA MPs in terms of sorption capacities and toxicity is so far limited and broadly unexplored since the current scientific emphasis has been merely centered on the conventional MPs' behavior. In this light, the present review provides an inclusive overview of the ongoing research of poly(lactic acid) in the framework of microplastics' pollution, while the future trends and missing points in this context are highlighted.

Microplastics in the environment: Sampling, pretreatment, analysis and occurrence based on current and newly-exploited chromatographic approaches.

The omnipresent character of microplastics (MPs) in environmental matrices, organisms and products has recently posed the need of their qualitative as well as quantitative analysis imperative, in order to provide data about their abundance and specification of polymer types in several substrates. In this framework, current and emerging approaches based on the chromatographic separation are of increased relevance in the field of MPs analysis and possess a large number of merits, since most of them are applicable in various complex matrices, sensitive and ideal for the detection of small-sized particles, whereas the common absence of any special pre-treatment step before analysis should also be highlighted. Αnalytical pyrolysis coupled with gas chromatography mass spectrometry (GC-MS) has recently gained ground as a powerful means to deliver information on MPs composition and degradation after their release into environment. Several instrumentations and trends in the area of analytical pyrolysis are thoroughly described within this review, while newly-exploited chromatographic methods in the field of MPs analysis, including Liquid Chromatography (LC) and Gel Permeation Chromatography (GPC) in this line are also investigated. The present review fills the gap of standardization concerning sampling, pre-treatment and chromatographic approaches and gathers all the available methodologies applied inside this area in accordance with the studied substrate, with the most examined environmental matrices being the solid one. After investigating the various works, some development options arise and it appears that chromatographic approaches should focus on improved extraction processes in terms of MPs isolation, since it is a crucial part in plastic items monitoring and is commonly depended on the polymer type and matrix. Special attention is given on the potential of chromatographic techniques for microplastics identification as well as quantification by confirming the current research status and knowledge gaps and highlighting some of the recent trends in this field.