RESUMEN
Recent studies have demonstrated that selective 5-HT(1F) receptor agonists inhibit neurogenic dural inflammation, a model of migraine headache, indicating that these compounds may be effective therapies for the treatment of migraine pain. This communication describes the synthesis and discovery of a novel compound, N-[3-(2-(dimethylamino)ethyl)-2-methyl-1H-indol-5-yl]-4-fluorobenzamide (4), which possesses high binding affinity and selectivity at the 5-HT(1F) receptor relative to more than 40 other serotonergic and nonserotonergic receptors examined.
Asunto(s)
Benzamidas/síntesis química , Indoles/síntesis química , Trastornos Migrañosos/tratamiento farmacológico , Receptores de Serotonina/efectos de los fármacos , Agonistas de Receptores de Serotonina/síntesis química , Animales , Benzamidas/química , Benzamidas/metabolismo , Benzamidas/farmacología , Línea Celular , Duramadre/efectos de los fármacos , Cobayas , Humanos , Técnicas In Vitro , Indoles/química , Indoles/metabolismo , Indoles/farmacología , Inflamación , Contracción Muscular/efectos de los fármacos , Músculo Liso Vascular/efectos de los fármacos , Músculo Liso Vascular/fisiología , Conejos , Ratas , Receptores de Neurotransmisores/metabolismo , Receptores de Serotonina/metabolismo , Vena Safena/efectos de los fármacos , Vena Safena/fisiología , Agonistas de Receptores de Serotonina/química , Agonistas de Receptores de Serotonina/metabolismo , Agonistas de Receptores de Serotonina/farmacología , Relación Estructura-Actividad , Receptor de Serotonina 5-HT1FRESUMEN
Solution-phase, parallel-synthesis techniques were used to optimize a series of nonbasic NK-1 antagonists, resulting in the identification of (R)-26, an orally bioavailable compound with subnanomolar potency.
Asunto(s)
Antagonistas del Receptor de Neuroquinina-1 , Relación Estructura-ActividadRESUMEN
The synthesis of allylic amine libraries derived from olefin templates is described. The two-step, solution phase reaction sequence consists of amination of the template followed by Suzuki coupling and expedited purification via ion exchange chromatography. The methodology has been used to synthesize a 1344-member allylic amine library.
Asunto(s)
Alquenos/química , Compuestos Alílicos/síntesis química , Aminas/síntesis química , Compuestos Alílicos/química , Aminas/química , Cromatografía por Intercambio Iónico , Técnicas Químicas Combinatorias , Indicadores y Reactivos , Espectroscopía de Resonancia Magnética , Espectrometría de MasasRESUMEN
Using a combination of iterative structure-based design and an analysis of oral pharmacokinetics and antiviral activity, AG1343 (Viracept, nelfinavir mesylate), a nonpeptidic inhibitor of HIV-1 protease, was identified. AG1343 is a potent enzyme inhibitor (Ki = 2 nM) and antiviral agent (HIV-1 ED50 = 14 nM). An X-ray cocrystal structure of the enzyme-AG1343 complex reveals how the novel thiophenyl ether and phenol-amide substituents of the inhibitor interact with the S1 and S2 subsites of HIV-1 protease, respectively. In vivo studies indicate that AG1343 is well absorbed orally in a variety of species and possesses favorable pharmacokinetic properties in humans. AG1343 (Viracept) has recently been approved for marketing for the treatment of AIDS.
Asunto(s)
Fármacos Anti-VIH/síntesis química , Fármacos Anti-VIH/farmacología , Inhibidores de la Proteasa del VIH/síntesis química , Inhibidores de la Proteasa del VIH/farmacología , VIH-1/enzimología , Nelfinavir/síntesis química , Nelfinavir/farmacología , Administración Oral , Animales , Fármacos Anti-VIH/farmacocinética , Disponibilidad Biológica , Callithrix , Perros , Relación Dosis-Respuesta a Droga , Femenino , Inhibidores de la Proteasa del VIH/farmacocinética , VIH-1/efectos de los fármacos , Macaca fascicularis , Masculino , Nelfinavir/farmacocinética , Ratas , Ratas Sprague-Dawley , Relación Estructura-ActividadRESUMEN
The serotonin (5-HT) receptor subtype mediating inhibition of neurogenic dural inflammation in guinea pigs was investigated using a series of serotonin agonists with differing affinities for the 5-HT1B, 5-HT1D and 5-HT1F receptors. When agonist potencies for inhibiting neurogenic inflammation were compared with affinities for these receptor subtypes, a significant positive correlation was seen only with the 5-HT1F receptor. The potency of agonists in inhibiting adenylate cyclase in cells transfected with human 5-HT1F receptor was also highly correlated with their potency in the animal model of migraine. In situ hybridization demonstrated 5-HT1F receptor mRNA in guinea pig trigeminal ganglion neurons. These data suggest that the 5-HT1F receptor is a rational target for migraine therapeutics.
Asunto(s)
Benzamidas/farmacología , Carbazoles/farmacología , Indoles/farmacología , Pirazoles/farmacología , Receptores de Serotonina/efectos de los fármacos , Agonistas de Receptores de Serotonina/farmacología , Ganglio del Trigémino/efectos de los fármacos , Animales , Modelos Animales de Enfermedad , Cobayas , Hibridación in Situ , Inflamación/tratamiento farmacológico , Masculino , Piperidinas/farmacología , ARN Mensajero/metabolismo , Conejos , Triptaminas , Receptor de Serotonina 5-HT1FRESUMEN
During the past several years, the field of combinatorial chemistry has expanded to include not only solid- and solution-phase methods for expedited compound synthesis, but also hybrid approaches that span these two extremes. In particular, polymer-supported reagents have emerged as useful combinatorial chemistry tools for the discovery and optimization of new pharmaceutical leads.
Asunto(s)
Química Orgánica , Indicadores y Reactivos/química , Polímeros/química , Fenómenos Químicos OrgánicosRESUMEN
We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.