RESUMEN
Polystyrene nanoplastics (PSNPs) when exposed to nanopermethrin (NPER) exacerbate toxicity on Artemia salina. In the environment, NPs act as a vector for other pollutants mainly heavy metals and pesticides. Nanopesticides are efficient compared to their bulk form. The adsorption of NPER on PSNPs was studied systematically and it was found that the binding of NPER is inversely proportional to its concentration. NPER adsorption on PSNPs followed pseudo-first-order kinetics with an adsorption percentage of 1.7%, 3.7%, 7.7%, 15.4%, and 30.8% when PSNPs were incubated with 2 mg L-1,4 mg L-1, 8 mg L-1, 16 mg L-1, and 32 mg L-1 of NPER. The adsorption followed the Langmuir isotherm. The increased hydrodynamic size of the NPER/PSNP complex was observed. Different characterization studies were performed for NPER, PSNPs, and their complex using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction, and gas chromatography-mass spectrometry. The LC50 value for the NPER/PSNP complex treated with Artemia salina was 3.127 mg L-1, compared to LC50 NPER which was found to be 4.536 mg L-1. PSNPs had a lower mortality rate in Artemia salina, where 50% mortality (LC50) was not observed at their working concentration. Both the nanoforms led to morphological changes in Artemia salina. Reactive oxygen species increased to 87.94% for the NPER/PSNP complex, 78.93% for NPER, and 23.65% for PSNPs. Greater amounts of ROS in the cells may have led to SOD degradation. Superoxide dismutase activity for the NPER/PSNP complex was 1.2 U mg-1, NPER was 1.3 U mg-1, and PSNPs was 2.1 U mg-1. A lipid peroxidation study reveals that the melondialdehyde synthesis by NPER/PSNPs complex, NPER and PSNPs were found to be 2.21 nM mg-1, 1.59 nM mg-1, and 0.91 nM mg-1 respectively. Catalase activity in a complex of NPER/PSNPs, NPER, and PSNPs was found to be 1.25 U mg-1, 0.94 U mg-1, and 0.49 U mg-1. This study envisages the individual and combined toxicity of nanopesticides and PSNPs on aquatic organisms. Increased plastic usage and new-age chemicals for agriculture could result in the formation of a PSNPs-NPER complex potentially causing highly toxic effects on aquatic animals, compared to their pristine forms. Therefore, we should also consider the other side of nanotechnology in agriculture.
RESUMEN
Despite a significant amount of research on micronanoplastics (MNPs), there is still a gap in our understanding of their function as transporters of other environmental pollutants (known as the Trojan horse effect) and the combined effects of ingestion, bioaccumulation, and toxicity to organisms. This study examined the individual effects of polystyrene nanoplastics (PSNPs) with various surface functionalizations (plain (PS), carboxylated (PS-COOH), and aminated (PS-NH2)), particle sizes (100 nm and 500 nm), and a pharmaceutical co-contaminant (metformin hydrochloride (MH), an anti-diabetic drug) on the marine crustacean - Artemia salina. The study specifically aimed to determine if MH alters the detrimental effects of PSNPs on A. salina. The potential toxicity of these emerging pollutants was assessed by examining mortality, hatching rate, morphological changes, and biochemical changes. Smaller nanoparticles had a more significant impact than larger ones, and PS-NH2 was more harmful than PS and PS-COOH. Exposure to the nanoparticle complex with MH resulted in a decrease in hatching rate, an increase in mortality, developmental abnormalities, an increase in reactive oxygen species, catalase, and lipid peroxidase, and a decrease in total protein and superoxide dismutase, indicating a synergistic effect. There were no significant differences between the complex and the individual nanoparticles. However, accumulating these particles in organisms could contaminate the food chain. These results highlight the potential environmental risks associated with the simultaneous exposure of aquatic species to plastics, particularly smaller PS, aminated PS, and pharmaceutical complex PS.
