RESUMEN
Statement of the Problem: The use of a new antimicrobial combination in the regenerative endodontic treatment of immature teeth pulp necrosis is a well-known method. Concerns have been raised about the destructive effect of this combination on the stem cells from the apical papilla of permanent human teeth, and there is a study gap. Purpose: The main objective of the present study was to investigate the cytotoxic effect of modified triple antibiotic paste (mTAP) on stem cells from the apical papilla (SCAPs) of permanent human teeth. Materials and Method: In this in vitro study, stem cells were removed from the immature teeth. After cultivation and third passage, metronidazole, ciprofloxacin, minocycline, and clindamycin were placed in the cell culture medium alone , paired, and in combinations as triple antibiotic paste (TAP) (metronidazole, ciprofloxacin, and minocycline) and mTAP (metronidazole, ciprofloxacin, clindamycin) with doses of 25, 50, 100, 200, 400µg/ml. After 1 and 3 days, cell viability in the culture medium was assessed using the MTT method ([4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). SPSS software version 24, descriptive statistics methods, and statistical tests such as Kruskal-Wallis and Mann-Whitney tests were adopted to analyze the data. Results: Analysis of MTT findings indicated that the use of mTAP at 100µg/ml and TAP at 200µg/ml had no adverse cytotoxic effect on stem cells in the first 24 hours, compared to the control group. The cell viability decreased at higher concentrations, although it was not statistically significant. After 72 hours, the toxicity of concentrations higher than 100µg/ml of mTAP and 400 µg/ml of TAP significantly mitigated the percentage of viable cells. Conclusion: The obtained results demonstrated that the concentration of 100 µg/ml of mTAP could replace TAP in regenerative endodontic treatments at the studied time intervals without worrying about the toxicity.
RESUMEN
Background and aims. Due to anatomic variation in tooth anatomy between populations, this study compared the buccolingual (BL) and mesiodistal (MD) dimensions of primary molars with those of stainless steel crowns (SSCs) in anIranian population. Materials and methods. Impressions were taken from both dental arches of children, and casts were poured. Teeth with caries, restoration, hypoplasia or other dental anomalies were excluded. 216 primary molars were selected and divided into 4 groups of 54 each (maxillary and mandibular first and second primary molars). MD/BL dimensions were measured using a digital caliper with 0.01 mm precision on casts and SCCs (3M brand). Data were assessed using paired t-test, post hoc test and ANOVA. P < 0.05 was considered statistically significant. Results. The MD dimension of the lower first molar SSC and the BL dimension of the lower second molar SSC had the least difference with the corresponding values of the respective teeth. The MD dimension of the upper second molar SSC and the BL dimension of the upper first molar SSC had the greatest difference with the corresponding values in the respective teeth. Comparison of the two different brands of SSCs for the upper first molar revealed that both types had significant differences with the teeth in terms of both MD (P = 0.0) and BL (P = 0.0) dimensions. Conclusion. In the studied population, best adaptation was seen in second lower molars and the least adaptationswere seen in first and second upper molars.