Oral management with polaprezinc solution reduces adverse events in haematopoietic stem cell transplantation patients.

The aim of this study was to analyse the effects of gargling with and then swallowing PPAA (polaprezinc in polyacrylic acid solution), in addition to regular oral management, on patients with a haematopoietic neoplasm scheduled for haematopoietic stem cell transplantation (HSCT). A total of 120 patients scheduled for HSCT during the years 2006-2016 were recruited. Patient background, oral adverse events, the incidence and severity of systemic adverse events (sepsis/septic shock, acute graft-versus-host disease (GVHD) after transplantation), and outcomes (survival/death) were compared between groups treated with and without PPAA. The severities of oral adverse events (oral mucositis, oral pain, and dysgeusia) were significantly lower in patients treated with PPAA. There was no significant difference in the incidence of febrile neutropenia (P=0.622) or sepsis/septic shock (P=0.665) as systemic adverse events. The severity of allograft-induced acute graft-versus-host disease (GVHD) was significantly lower in the PPAA group (P=0.011). There was no significant difference in outcome between the two groups (P=0.285). Within the limitations of the study design, it may be concluded that oral management with PPAA reduces adverse events in HSCT. Oral management with concomitant use of PPAA decreased oral adverse events and reduced the systemic complication of GVHD.

Desempenho e características bioquímicas de leitões submetidos a dietas com aditivos probióticos, prebióticos, simbióticos e antibióticos / Performance and biochemical parameters of piglets and the use of alternative additives as antibiotic replacement

Avaliaram-se o desempenho e as características bioquímicas de leitões desmamados, utilizando-se aditivos alternativos em substituição aos antibióticos. Foram utilizados 480 leitões machos, distribuídos em delineamento inteiramente ao acaso, composto por cinco tratamentos. A ração foi à base de milho, farelo de soja e um núcleo comercial para leitões. Os tratamentos consistiram em T1: ração sem aditivos, T2: dieta basal com antibiótico, T3: dieta basal com adição de prebiótico, T4: dieta basal com adição de probiótico e T5: dieta basal com adição de simbiótico. Foram avaliados peso corporal, ganho de peso, consumo de ração e conversão alimentar. Em 50 amostras de sangue de leitões, sendo 10 amostras de cada tratamento, avaliaram-se as concentrações séricas de proteínas totais, de albumina e globulina, a relação albumina:globulina e a fosfatase alcalina, creatinina, alanina aminotransferase, aspartato aminotransferase, gama- glutamiltransferase, colesterol, ureia, cálcio e fósforo e a relação cálcio:fósforo. A substituição de antibióticos por aditivos alternativos não influenciou o desempenho nem as características bioquímicas dos leitões.

The experiment was conducted to evaluate the performance and biochemical parameters in weaned piglets using alternative supplements to replace antibiotics. A total of 480 male pigs were used, randomly distributed in delineation with five treatments. The feed was based on corn, soybean meal and a commercial core for piglets. The treatments were T1: diet without supplements or basal diet, T2: basal diet with antibiotics, T3: basal diet with added probiotic, T4: basal diet with added probiotic and T5: basal diet with addition of symbiotic. In the experiment we evaluated performance characteristics, body weight, weight gain, feed intake and feed conversion. In the second part of the experiment, we evaluated the biochemical parameters of piglets used in the first experiment, fifty blood samples, ten samples of each treatment, were taken, and after separation of the clot, the serum was frozen at -20°C. Subsequently we analyzed the serum levels of total protein, albumin, globulin, albumin/globulin, ALP, creatinine, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, cholesterol, urea, calcium, phosphorus and calcium/phosphorus ratio. No significant differences in biochemical parameters or in the performance of piglets submitted to different diets were found.

Pulmonary atherosclerosis and pulmonary arterial pressure in cholesterol-fed New Zealand white rabbits.

The lung produces many vasoactive substances originating from its vascular endothelium and plays an important part in various pathose. The present study was carried out to clarify pulmonary atherosclerosis and pulmonary arterial pressure, and to elucidate a part of the pulmonary pathosis in cholesterol-fed rabbits. Atherosclerosis was induced by feeding the animals a cholesterol-rich diet. When the rabbits were fed the cholesterol-enriched diets for 15 weeks, the grade of the atherosclerosis was severer than in 8W-feeding rabbits. The lesions of 8W-feeding rabbits were mainly composed of foam cells and fibrous components, whereas in 15W-feeding rabbits, the aggregation of foam cells beneath the endothelium of the vessel was infiltrating the media and severe stenose of the lumen was observed. In the entire pulmonary arterial system, the severe obstructive vascular lesions were localized and not diffused. The pulmonary arterial pressures of the rabbits increased slightly with time and the mean pressures were 11.3+/-0.9 (control group), 11.8+/-1.0 (8W group) and 13.7+/-1.5 mmHg (15W group) respectively. A significant difference existed in the mean pressure between the control group and 15W-feeding group, but there were no significant differences in the systolic and diastolic pressures among the three groups. In conclusion, we could induce pulmonary atherosclerosis in rabbits by feeding them a hyper-cholesterol diet but not overt pulmonary hypertension.

Arteriosclerosis in the influx and intravisceral arteries of the liver, kidney and lung of WHHL rabbits.

We performed a histopathological investigation on arteriosclerotic development in the influx and intravisceral arteries of the liver, kidney and lung of male WHHL rabbits. In the influx arteries of these organs, we observed severe atherosclerotic vascular lesions with high-grade luminal stenosis. In the intravisceral arteries of the liver and kidney, no arteriosclerotic lesions were observed. However, in the intrapulmonary arteries, we recognized severe atherosclerotic vascular changes with high-grade stenosis or total obstruction of the lumen in some middle to large sized pulmonary arteries. These observations indicate that the development of arteriosclerosis in parenchymatous organs differs, and that some organs are predisposed to arteriosclerosis formation.

Mining of biological data I: identifying discriminating features via mean hypothesis testing.

Large volumes of data are routinely collected during bioprocess operations and, more recently, in basic biological research using genomics-based technologies. While these data often lack sufficient detail to be used for mechanism identification, it is possible that the underlying mechanisms affecting cell phenotype or process outcome are reflected as specific patterns in the overall or temporal sensor logs. This raises the possibility of identifying outcome-specific fingerprints that can be used for process or phenotype classification and the identification of discriminating characteristics, such as specific genes or process variables. The aim of this work is to provide a systematic approach to identifying and modeling patterns in historical records and using this information for process classification. This approach differs from others in that emphasis is placed on analyzing the data structure first and thereby extracting potentially relevant features prior to model creation. The initial step in this overall approach is to first identify the discriminating features of the relevant measurements and time windows, which can then be subsequently used to discriminate among different classes of process behavior. This is achieved via a mean hypothesis testing algorithm. Next, the homogeneity of the multivariate data in each class is explored via a novel cluster analysis technique called PC1 Time Series Clustering to ensure that the data subsets used accurately reflect the variability displayed in the historical records. This will be the topic of the second paper in this series. We present here the method for identifying discriminating features in data via mean hypothesis testing along with results from the analysis of case studies from industrial fermentations

Mining of biological data II: assessing data structure and class homogeneity by cluster analysis.

An important step in data analysis is class assignment which is usually done on the basis of a macroscopic phenotypic or bioprocess characteristic, such as high vs low growth, healthy vs diseased state, or high vs. low productivity. Unfortunately, such an assignment may lump together samples, which when derived from a more detailed phenotypic or bioprocess description are dissimilar, giving rise to models of lower quality and predictive power. In this paper we present a clustering algorithm for data preprocessing which involves the identification of fundamentally similar lots on the basis of the extent of similarity among the system variables. The algorithm combines aspects of cluster analysis and principal component analysis by applying agglomerative clustering methods to the first principal component of the system data matrix. As part of a rational strategy for developing empirical models, this technique selects lots (samples) which are most appropriate for inclusion in a training set by analyzing multivariate data homogeneity. Samples with similar data structures are identified and grouped together into distinct clusters. This knowledge is used in the formation of potential training sets. Additionally, this technique can identify atypical lots, i.e., samples that are not simply outliers but exhibit the general properties of one class but have been given the assignment of the other. The method is presented along with examples from its application to fermentation data sets.

Surgical management of early stage central (hilar) and peripheral nonsmall cell lung carcinoma.

BACKGROUND: To the authors' knowledge, the definitions of early lung carcinoma used in Japan are not yet approved internationally. In the current study the diagnostic methods, pathology, and results of the surgical treatment of early hilar and peripheral nonsmall cell lung carcinoma cases defined by Japanese criteria are presented. METHODS: Between 1977 and 1998, 43 patients with early hilar lung carcinoma were encountered. These patients constituted 14.5% of 200 hilar lung carcinoma patients who underwent resection during the same period. Between 1973 and 1998, there were 174 early peripheral lung carcinomas, which comprised 14.8% of 1173 peripheral lung carcinoma patients who underwent resection during the same period. RESULTS: The 5-year and 10-year survival rates of the early hilar lung carcinoma were 100% and 96.9%, respectively. The majority of the patients underwent bronchoplastic surgery, such as sleeve lobectomy and parenchymal-sparing surgery including seven sleeve segmentectomies and two second carinal resections. The effectiveness of the parenchymal-sparing resections was assessed by the conventional pulmonary function test as well as perfusion and ventilation scanning. There were 174 cases of peripheral early lung carcinoma among the 218 cases of peripheral lung carcinoma with tumor dimensions of < or = 2 cm. The cell types were 142 (81.6%) cases of adenocarcinoma, 18 (10.3%) cases of squamous cell carcinoma, and 14 (8%) cases of other cell types. These 174 patients comprised 14.8% of 1173 patients with peripheral nonsmall cell lung carcinoma who underwent surgery during the same period. The 5-year and 10-year survival rates for adenocarcinoma were 89.6% and 86.8%, respectively. The 5-year and 10-year survival rates for other cell types were both 71.8%. The overall 5-year and 10-year survival rates were 86.8% and 84.6%, respectively. CONCLUSIONS: Early lung carcinoma in both the hilar and peripheral regions, is curable if it is properly diagnosed and treated.

Salt water promotes hypertension in Dahl-S rats.

Hypertension was induced in Dahl-salt-sensitive (Dahl-S) rats by administering salt in drinking water. Control rats receiving tap water did not show a significant change in blood pressure or abnormalities in the kidney. Rats receiving 0.5% NaCl solution developed moderate hypertension and renal lesions. Rats receiving 1.0% NaCl solution showed prominent and increasing hypertension and severe renal damage. This method of salt administration should be simpler than administration in the diet as a means of promoting renal hypertension. The lower concentration salt water caused chronic mild hypertension in Dahl-S rats, and may serve as a useful model for progressive hypertension.

Expression of cholesterol 7alpha-hydroxylase and delta(4)-3-ketosteroid 5beta-reductase genes in rat pancreatic hepatocyte-like cells.

Hepatocyte-like cells have been observed in the pancreas of the rat. We examined the bile acid biosynthetic function of these cells to determine whether they were real hepatocytes. This study investigated the existence of two liver-specific enzymes involved in bile acid biosynthesis (cholesterol 7alpha-hydroxylase and delta(4)-3-ketosteroid 5beta-reductase) in the hepatocyte-like cells. We could demonstrate cholesterol 7alpha-hydroxylase activity and its circadian rhythm in the hepatocyte-like cells. Northern blot analysis demonstrated the expression of messenger RNA for the 7alpha-hydroxylase and delta(4)-3-ketosteroid 5beta-reductase in the pancreatic hepatocyte-like cells. To measure the amount of the messenger RNA, we used the competitive polymerase chain reaction method for the 7alpha-hydroxylase. This quantitation revealed the existence of a circadian rhythm of cholesterol 7alpha-hydroxylase messenger RNA in the hepatocyte-like cells. These results indicated that bile acid biosynthesis was performed in the pancreatic hepatocyte-like cells as noted as in the liver parenchymal cells.

Development of atherosclerotic lesions in cholesterol-loaded rabbits.

To examine both of the target vessels and the optimal time of their endothelial denudation to study vascular restenosis after balloon injury in cholesterol-loaded rabbits, we made 36 atherosclerotic rabbits by feeding a hypercholesterol diet, and histologically examined the onset time and the development of atherosclerosis. Atheromatous changes were observed first after the 5th week in the thoracic aorta from the start of the diet, and then extended to the abdominal aorta, coronary artery with time. The atherosclerotic lesions in the thoracic aorta and the proximal portion of the coronary artery showed high-grade concentric intimal thickening with luminal stenosis. The abdominal aortic lesion mildly progressed. In the renal, carotid and femoral arteries, in contrast, slight atheroscleromatous changes developed during the diet period. These results suggest that the thoracic and abdominal aortas and the coronary artery would be suitable as target vessels to study vascular restenosis after balloon injury, and the endothelial denudation of these vessels should be performed between the 8th and 15th week in this diet protocol for an accurate analysis.

Growth induction of rat primary hepatocytes using antisense oligonucleotides.

We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S-oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 microM. For the evaluation of growth, 3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.

Division of donor liver for successful split-liver transplantation in pigs.

Split Liver Transplantation (SLT) is an attractive method to solve the problem of a shortage of liver grafts. A through knowledge of the anatomy of the porcine liver vessels and bile duct is essential in performing the experimental SLT. This study was undertaken to decide the split line for successful SLT in pigs by examining the main branching patterns both vessels and bile duct in 30 porcine livers macroscopically and angiographically. The hepatic arterial branching patterns were divided into three types and bile duct patterns into two types. There was no exception in branching patterns of the portal vein and the hepatic vein. We conclude it is desirable that the donor liver should be divided into two grafts between the left medial lobe and quadrate lobe.

Fermentation database mining by pattern recognition.

A large volume of data is routinely collected during the course of typical fermentation and other processes. Such data provide the required basis for process documentation and occasionally are also used for process analysis and improvement. The information density of these data is often low, and automatic condensing, analysis, and interpretation ("database mining") are highly desirable. In this article we present a methodology whereby process variables are processed to create a database of derivative process quantities representative of the global patterns, intermediate trends, and local characteristics of the process. A powerful search algorithm subsequently attempts to extract the specific process variables and their particular attributes that uniquely characterize a class of process outcomes such as high- or low-yield fermentations.The basic components of our pattern recognition methodology are described along with applications to the analysis of two sets of data from industrial fermentations. Results indicate that truly discriminating variables do exist in typical fermentation data and they can be useful in identifying the causes or symptoms of different process outcomes. The methodology has been implemented in a user-friendly software, named db-miner, which facilitates the application of the methodology for efficient and speedy analysis of fermentation process data. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 443-452, 1997.

Partially covered Gianturco stent for tracheobronchial stricture caused by intraluminal tumor.

Partially covered Gianturco stents were used successfully in two patients to treat tracheobronchial strictures caused by intraluminal tumor growth. The stents were accurately placed without complications. The covered portion of the stent compressed the tumor, and prevented tumor ingrowth until the patients' deaths. This stent seems to act as effective palliation for tracheobronchial stricture caused by intraluminal tumor.

Branching patterns in coronary artery and ischemic areas induced by coronary arterial occlusion in the CLAWN miniature pig.

This study of 28 CLAWN miniature pigs (male 17, female 11, mean weight 29 kg) was undertaken to investigate the coronary arterial branching patterns and the ischemic area induced by surgical occlusion of the left anterior descending artery (LAD) and change in the ischemic area over time. These results were compared with those in dogs, which have frequently been used in myocardial ischemic research. Regarding the coronary arterial branching pattern, there were fewer ventricular branches from the right and left coronary arteries than in dogs. The septal branches arose from only the LAD and the posterior descending artery (PD). The largest septal artery branched from the LAD. There were two types of septal artery branching patterns. In approximately 80% of the CLAWN miniature pigs, the PD arose from the right coronary artery (Right dominance). The peculiarity of the coronary arterial branching pattern in the CLAWN miniature pigs was more similar to human beings than to dogs. The ischemic area induced by occlusion at three-fifths distal section of the LAD was 12.1% to 22.6% (mean 17.1%) of the left ventricle. The ischemic area in all animals that died of global left ventricular malfunction and hemodynamic instability after LAD occlusion was more than 25% of the left ventricle.

Knowledge-based systems, artificial neural networks and pattern recognition: applications to biotechnological processes.

Recent years have witnessed the increasing application of artificial intelligence techniques, specifically, knowledge-based systems, artificial neural networks, and pattern recognition, to biotechnological processes. Although progress has been made in simple control applications, more work is needed to establish the advantages of these techniques for industrial process control, for diagnosis/monitoring, and to upgrade the information content of historical data.

[Screening CT of the lungs: image quality compared with conventional CT].

More sensitive imaging methods may be required in the screening of early peripheral lung cancer. Among new radiologic techniques, helical volumetric computed tomography (CT) seems to be attractive. The image quality of helical CT with single breath-hold and low-dose technique (screening CT) was compared with conventional sequential CT in 12 patients to detect the pulmonary metastases. The screening CT protocol consisted of a 20 mm/sec table feed during a 20-second breath-hold at 40 mA, with reconstruction of images at 10 mm intervals. Conventional CT was performed with a 1-second scan time, 200 mA, and contiguous 10 mm-thick sections. In the evaluation of mediastinal vessels, bronchi and pulmonary nodules, screening CT showed high detectability images comparable to conventional CT. But subsegmental bronchi or small nodules overlapping pulmonary vessels were more difficult to visualize by screening CT because of image noise. The authors conclude that although further improvement is necessary, screening CT of the lung is an attractive new application of CT.

Fine structure of the parotid gland in tree shrew (Tupaia glis).

The parotid glands of Tupaia glis were examined by light and transmission electron microscopy. The acinar cells were seromucous in nature, and contained many acidophilic granules with strong affinity for periodic acid-Schiff (PAS) and weak affinity for alcian blue (AB). These granules consisted of a fine granular matrix of moderate density in which a denser corpuscles or semilunar materials were present. Intercalated duct cells had a few fine vesicles, vacuoles and very few dense granules in the apical region. In occasional epithelial cells, acidophilic, PAS-positive and AB-negative bodies with moderate density were observed in the supranuclear region. The striated ducts consisted of columnar light and dark cells containing round or small ovoid granules of moderate density and did not show the granular duct as seen in the parotid glands of kobe mole and tenrec which are placed in the order insectivora.

Clinical application of single dual-energy subtraction technique with digital storage-phosphor radiography.

We used a single-exposure dual-energy subtraction technique with a storage-phosphor computed radiography (CR) system to evaluate various pulmonary diseases. Clinical applications of this method have been studies at our institution. In our experience, the dual-energy subtraction technique provided better visualization of pulmonary nodules, tracheobronchial abnormalities, pulmonary vascular diseases, calcification in a nodule, and rib lesions than conventional CR images did. Single-exposure dual-energy subtraction images were obtained with a sandwich technique using two storage-phosphor imaging plates with a 1-mm-thick copper filter interleaved between the two. The front plate obtains a lower kilovoltage image, whereas the back plate obtains a higher kilovoltage image. We can visualize soft tissue and bone images as well as unsubtracted digital images by the subtraction process.