RESUMEN
We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the hybridization of the target with binary DNA probes. The new method was successfully exploited for SNP genotyping of human CYP2C19 gene.
Asunto(s)
Aptámeros de Nucleótidos , Sondas de ADN , ADN/química , Polimorfismo de Nucleótido Simple , Ácido Cólico/química , Citocromo P-450 CYP2C19/genética , Técnicas de Genotipaje , Humanos , Hibridación de Ácido NucleicoRESUMEN
A simple and rapid method for the detection of single nucleotide polymorphisms (SNPs) is essential for the development of personalized medicine because SNPs correlate with some diseases and side effects of some drugs. Here we report a new method for the fluorescent detection of single nucleotide mutations that is based on the formation of cholic-acid binding DNA aptamers which form fully matched three-way junctions.