Effectiveness of a fixed-depth sensor deployed from a buoy to estimate water-column cyanobacterial biomass depends on wind speed.

Water quality sondes have the advantage of containing multiple sensors, extended deployment times, high temporal resolution, and telecommunication with stakeholder accessible data portals. However, sondes that are part of buoy deployments often suffer from typically being fixed at one depth. Because water treatment plants are interested in water quality at a depth of the water intake and other stakeholders (ex. boaters and swimmers) are interested in the surface, we examined whether a fixed depth of approximately 1 m could cause over- or under-estimation of cyanobacterial biomass. We sampled the vertical distribution of cyanobacteria adjacent to a water quality sonde buoy in the western basin of Lake Erie during the summers of 2015-2017. A comparison of buoy cyanobacteria RFU (Relative Fluorescence Unit) at 1 m to cyanobacteria chlorophyll a (chla) measured throughout the water column showed occurrences when the buoy both under and overestimated the cyanobacteria chla at specific depths. Largest differences between buoy measurements and at-depth grab samples occurred during low wind speeds (< 4.5 m/sec) because low winds allowed cyanobacteria to accumulate at the surface above the buoy's sonde. Higher wind speeds (> 4.5 m/sec) resulted in better agreement between the buoy and at-depth measurements. Averaging wind speeds 12 hr before sample collection decreased the difference between the buoy and at-depth samples for high wind speeds but not low speeds. We suggest that sondes should be placed at a depth of interest for the appropriate stakeholder group or deploy sondes with the ability to sample at various depths.

Physical drivers facilitating a toxigenic cyanobacterial bloom in a major Great Lakes tributary.

The Maumee River is the primary source for nutrients fueling seasonal Microcystis-dominated blooms in western Lake Erie's open waters though such blooms in the river are infrequent. The river also serves as source water for multiple public water systems and a large food services facility in northwest Ohio, USA. On 20 September 2017, an unprecedented bloom was reported in the Maumee River estuary within the Toledo metropolitan area, which triggered a recreational water advisory. Here we (1) explore physical drivers likely contributing to the bloom's occurrence, and (2) describe the toxin concentration and bacterioplankton taxonomic composition. A historical analysis using ten-years of seasonal river discharge, water level, and local wind data identified two instances when high-retention conditions occurred over ≥10 days in the Maumee River estuary: in 2016 and during the 2017 bloom. Observation by remote sensing imagery supported the advection of cyanobacterial cells into the estuary from the lake during 2017 and the lack of an estuary bloom in 2016 due to a weak cyanobacterial bloom in the lake. A rapid-response survey during the 2017 bloom determined levels of the cyanotoxins, specifically microcystins, in excess of recreational contact limits at sites within the lower 20 km of the river while amplicon sequencing found these sites were dominated by Microcystis. These results highlight the need to broaden our understanding of physical drivers of cyanobacterial blooms within the interface between riverine and lacustrine systems, particularly as such blooms are expected to become more prominent in response to a changing climate.

Accuracy of data buoys for measurement of cyanobacteria, chlorophyll, and turbidity in a large lake (Lake Erie, North America): implications for estimation of cyanobacterial bloom parameters from water quality sonde measurements.

Microcystin (MCY)-producing harmful cyanobacterial blooms (cHABs) are an annual occurrence in Lake Erie, and buoys equipped with water quality sondes have been deployed to help researchers and resource managers track cHABs. The objective of this study was to determine how well water quality sondes attached to buoys measure total algae and cyanobacterial biomass and water turbidity. Water samples were collected next to two data buoys in western Lake Erie (near Gibraltar Island and in the Sandusky subbasin) throughout summers 2015, 2016, and 2017 to determine correlations between buoy sonde data and water sample data. MCY and nutrient concentrations were also measured. Significant (P < 0.001) linear relationships (R2 > 0.75) occurred between cyanobacteria buoy and water sample data at the Gibraltar buoy, but not at the Sandusky buoy; however, the coefficients at the Gibraltar buoy differed significantly across years. There was a significant correlation between buoy and water sample total chlorophyll data at both buoys, but the coefficient varied considerably between buoys and among years. Total MCY concentrations at the Gibraltar buoy followed similar temporal patterns as buoy and water sample cyanobacterial biomass data, and the ratio of MCY to cyanobacteria-chlorophyll decreased with decreased ambient nitrate concentrations. These results suggest that buoy data are difficult to compare across time and space. Additionally, the inclusion of nitrate concentration data can lead to more robust predictions on the relative toxicity of blooms. Overall, deployed buoys with sondes that are routinely cleaned and calibrated can track relative cyanobacteria abundance and be used as an early warning system for potentially toxic blooms.