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1.
BMC Med Imaging ; 24(1): 242, 2024 Sep 16.
Artículo en Inglés | MEDLINE | ID: mdl-39285357

RESUMEN

OBJECTIVES: To establish a nomogram for differentiating malignant and benign focal liver lesions (FLLs) using ultrasomics features derived from contrast-enhanced ultrasound (CEUS). METHODS: 527 patients were retrospectively enrolled. On the training cohort, ultrasomics features were extracted from CEUS and b-mode ultrasound (BUS). Automatic feature selection and model development were performed using the Ultrasomics-Platform software, outputting the corresponding ultrasomics scores. A nomogram based on the ultrasomics scores from artery phase (AP), portal venous phase (PVP) and delayed phase (DP) of CEUS, and clinical factors were established. On the validation cohort, the diagnostic performance of the nomogram was assessed and compared with seniorexpert and resident radiologists. RESULTS: In the training cohort, the AP, PVP and DP scores exhibited better differential performance than BUS score, with area under the curve (AUC) of 84.1-85.1% compared with the BUS (74.6%, P < 0.05). In the validation cohort, the AUC of combined nomogram and expert was significantly higher than that of the resident (91.4% vs. 89.5% vs. 79.3%, P < 0.05). The combined nomogram had a comparable sensitivity with the expert and resident (95.2% vs. 98.4% vs. 97.6%), while the expert had a higher specificity than the nomogram and the resident (80.6% vs. 72.2% vs. 61.1%, P = 0.205). CONCLUSIONS: A CEUS ultrasomics based nomogram had an expert level performance in FLL characterization.


Asunto(s)
Medios de Contraste , Neoplasias Hepáticas , Nomogramas , Ultrasonografía , Humanos , Femenino , Masculino , Persona de Mediana Edad , Ultrasonografía/métodos , Neoplasias Hepáticas/diagnóstico por imagen , Estudios Retrospectivos , Diagnóstico Diferencial , Adulto , Anciano , Sensibilidad y Especificidad , Hígado/diagnóstico por imagen
2.
Int J Colorectal Dis ; 39(1): 142, 2024 Sep 17.
Artículo en Inglés | MEDLINE | ID: mdl-39289219

RESUMEN

OBJECTIVE: The aim of this study is to evaluate the significance of combined detection of Septin9 and syndecan-2 (SDC2) methylation markers and serum tumor markers for the early diagnosis of colorectal cancer. METHODS: A total of 116 patients diagnosed with colorectal cancer between December 2022 and February 2024 were designated as the colorectal cancer group. Additionally, 31 patients with colorectal adenoma were assigned to the adenoma group, while 44 individuals undergoing routine physical examinations were included in the control group. Concentrations of Septin9, SDC2, fecal occult blood (FOB), and four tumor markers-carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), carbohydrate antigen 125 (CA125), and carbohydrate antigen 724 (CA724)-were measured. Diagnostic performance was assessed using receiver operating characteristic (ROC) curves for Septin9, SDC2, the four tumor markers, FOB, the combination of Septin9 and SDC2, and the combined use of all seven indicators (CEA, CA19-9, CA125, CA72-4, FOB, Septin9, and SDC2). RESULTS: The colorectal cancer group exhibited the highest positive rates for Septin9, SDC2, the four tumor markers, the combined detection of Septin9 and SDC2, and the combined detection of all seven indicators, compared to both the adenoma and control groups (P < 0.05). The adenoma group also showed higher positive rates than the control group (P < 0.05). For patients with stage I-III colorectal cancer, the positive rates for the combined detection of Septin9 and SDC2 were 81.3%, 78.9%, and 90.2%, respectively, surpassing those for the combined detection of the four tumor markers (43.8%, 55.3%, and 61.0%). Additionally, the positive rates for the two-gene combination in stage III colorectal cancer were higher than those for FOB (P < 0.05). The sensitivity and area under the curve (AUC) for SDC2 were 73.3% and 0.855, respectively, exceeding the sensitivity and AUC for the combined four tumor markers, which were 60.3% and 0.734 (P < 0.05). The combined detection of the two methylated genes demonstrated a sensitivity of 86.2% and an AUC of 0.908, outperforming both FOB and the combined detection of the four tumor markers (P < 0.05). CONCLUSION: The detection of SDC2 exhibits high sensitivity for colorectal cancer, and when combined with Septin9, it significantly enhances the diagnostic accuracy for early-stage colorectal cancer, offering substantial clinical value.


Asunto(s)
Biomarcadores de Tumor , Neoplasias Colorrectales , Detección Precoz del Cáncer , Septinas , Sindecano-2 , Humanos , Septinas/sangre , Septinas/genética , Sindecano-2/sangre , Neoplasias Colorrectales/sangre , Neoplasias Colorrectales/diagnóstico , Biomarcadores de Tumor/sangre , Femenino , Masculino , Persona de Mediana Edad , Detección Precoz del Cáncer/métodos , Anciano , Curva ROC , Adulto , Sangre Oculta
3.
Opt Lett ; 49(15): 4154-4157, 2024 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-39090882

RESUMEN

Due to their ultrahigh Q-factor and small mode volume, bound states in the continuum (BICs) are intriguing for the fundamental study of the strong coupling regime. However, the strong coupling generated by BICs in one metasurface is not always strong enough, which highly limits its efficiency in applications. In this work, we realize a giant strong coupling of at most 60 meV in a quasi-BICs' (Q-BICs) tetramer metasurface composed of four Si cylinders with two different sets of diagonal lengths. The Q-BICs are induced from two types of electric quadrupole (EQ), for which detuning can be flexibly controlled by manipulating the C4v symmetry breaking Δd. The giant Rabi splitting in our proposed metasurface performs more than 15 times of the previous works, which provides more possibilities for important nonlinear and quantum applications, such as nanolaser and quantum optics.

4.
ACS Appl Mater Interfaces ; 16(25): 32058-32077, 2024 Jun 26.
Artículo en Inglés | MEDLINE | ID: mdl-38872401

RESUMEN

The development of growth factor-free biomaterials for bone tissue regeneration with anti-infection and anti-inflammatory activities remains challenging. Black phosphorus nanosheets (BPNs), with distinctive attributes, including photothermal conversion and calcium ion chelation, offer potential for use in bone tissue engineering and infection prevention. However, BPNs are prone to oxidation and degradation in aqueous environments, and methods to stabilize BPNs for long-term bone repair remain insufficient. Herein, zeolitic imidazolate framework-8 (ZIF-8) was used to stabilize BPNs via in situ crystallization onto the surface of BPNs (BP@ZIF-8 nanocomposite). A novel injectable dual-component hydrogel comprising gelatin methacryloyl (GelMA) and methacrylate-modified hyaluronic acid (HAMA) was used as a BP@ZIF-8 nanocomposite carrier (GelMA/HAMA/BP@ZIF-8). The BP@ZIF-8 nanocomposite could effectively protect internal BPNs from oxidation and enhance the long-term photothermal performance of the hydrogel in both in vitro and in vivo settings. The GelMA/HAMA/BP@ZIF-8 hydrogel was injectable and exhibited outstanding performance for photothermal conversion, mechanical strength, and biodegradability, as well as excellent photothermal antibacterial activity against Staphylococcus aureus and Escherichia coli in vitro and in an in vivo rat model. The GelMA/HAMA/BP@ZIF-8 hydrogel also provided a microenvironment conducive to osteogenic differentiation, promoting the transformation of M2 macrophages and inhibiting inflammatory responses. Furthermore, the hydrogel promoted bone regeneration and had a synergistic effect with near-infrared irradiation in a rat skull-defect model. Transcriptome sequencing analysis revealed that the PI3K-AKT- and calcium-signaling pathways may be involved in promoting osteogenic differentiation induced by the GH-BZ hydrogel. This study presents an innovative, multifaceted solution to the challenges of bone tissue regeneration with antibacterial and anti-inflammatory effects, providing insights into the design of smart biomaterials with dual therapeutic capabilities.


Asunto(s)
Antibacterianos , Escherichia coli , Hidrogeles , Osteogénesis , Fósforo , Staphylococcus aureus , Zeolitas , Antibacterianos/química , Antibacterianos/farmacología , Hidrogeles/química , Hidrogeles/farmacología , Animales , Osteogénesis/efectos de los fármacos , Fósforo/química , Escherichia coli/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Ratas , Zeolitas/química , Zeolitas/farmacología , Gelatina/química , Gelatina/farmacología , Ratones , Ácido Hialurónico/química , Ácido Hialurónico/farmacología , Ratas Sprague-Dawley , Metacrilatos/química , Metacrilatos/farmacología , Pruebas de Sensibilidad Microbiana , Estructuras Metalorgánicas/química , Estructuras Metalorgánicas/farmacología , Nanocompuestos/química , Células RAW 264.7 , Regeneración Ósea/efectos de los fármacos , Nanoestructuras/química
5.
Huan Jing Ke Xue ; 45(2): 992-1003, 2024 Feb 08.
Artículo en Chino | MEDLINE | ID: mdl-38471937

RESUMEN

The process of vegetation restoration is often accompanied by significant changes in aboveground plant diversity. To explore the driving mechanism of litter nutrient-soil nutrient-enzyme activity stoichiometry on aboveground vegetation change is of great importance for maintaining regional biodiversity conservation and ecological stability. Taking typical abandoned farmland of different restoration years (1, 8, 16, 31, and 50 a) in the Qinling Mountains as the research object, the variation characteristics of plant community diversity during vegetation restoration were analyzed through field investigation. Litter nutrients, soil nutrients, and the activities of five extracellular enzymes, including ß-1,4-glucosidase (BG), cellobiohydrolase (CBH), ß-1,4-N-acetylglucosaminidase (NAG), leucine aminopeptidase (LAP), and acid phosphatase (AP), were determined. The characteristics of litter nutrients, soil nutrients, and enzyme stoichiometric ratios during vegetation restoration and the driving mechanism of plant diversity changes were discussed. The results showed that the plant community diversity index firstly decreased and then increased with the increase in vegetation restoration years, and the minimum was reached at 16 years after restoration. The results of principal component analysis showed that there were significant differences between total plant community diversity index and litter-soil-enzyme stoichiometric characteristics in different years of vegetation restoration. The plant community diversity index had a strong positive correlation with litter C∶P ratio and litter N∶P ratio but had a negative correlation with soil enzyme C∶P ratio (EEA C∶P). The results of redundancy analysis showed that soil EEA C∶P had the highest explanation rate of plant diversity changes during vegetation restoration (25.93%), followed by soil TP (5.94%), which was the key factor regulating plant diversity changes. In conclusion, plant species and quantity increased significantly in abandoned farmland in the middle part of the Qinling Mountains at the late stage of vegetation restoration. Changes in the soil environment affected microbial metabolic activities and thus changed enzyme activities. Litter-soil-soil extracellular enzymes affected the community environment and plant diversity through feedback and regulation. EEA C∶P and TP were the main driving factors of aboveground plant diversity change during vegetation restoration.


Asunto(s)
Biodiversidad , Plantas , Suelo , Microbiología del Suelo , Nutrientes , Ecosistema , China
6.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-1024097

RESUMEN

Objective To assess the risk factors for carbapenem-resistant Acinetobacter baumannii(CRAB)bloodstream infection(BSI)and 28-day short-term mortality in elderly patients,and provide reference for the pre-vention and treatment of CRAB BSI.Methods Clinical data of patients aged ≥60 years and diagnosed with AB BSI in a hospital in Yulin City from January 2013 to December 2022 were retrospectively analyzed,including demogra-phic and microbiological characteristics,as well as clinical outcomes of the patients.Variables which were significant in univariate analysis were selected for multivariate analysis using binary logistic regression model and Cox propor-tional hazards model.Independent risk factors for infection were further determined,and survival analysis was per-formed using Kaplan-Meier curve.Results A total of 150 patients were included in the study,out of which 16 pa-tients(10.7%)had CRAB BSI and 134 had carbapenem-sensitive AB(CSAB)BSI.The 28-day short-term mortali-ty of AB BSI in elderly patients was 15.3%(23/150,95%CI:9.6%-21.1%),and the short-term mortality of CRAB BSI was higher than that of CSAB([56.3%,9/16]vs[10.4%,14/134]).Deep venous catheterization(OR:15.598,95%CI:1.831-132.910)and combined infections of other sites(OR:15.449,95%CI:1.497-159.489)were related to CRAB BSI in elderly patients.The independent risk factors for 28-day mortality in elderly patients with AB BSI were hemodialysis(OR:11.856,95%CI:2.924-48.076),intensive care unit admission(OR:9.387,95%CI:1.941-45.385),and pulmonary infection being suspected source of bacteremia(OR:7.019,95%CI:1.345-36.635).Conclusion The occurrence of CRAB BSI in elderly patients is related to the combined infection of other sites and deep vein catheterization.Hemodialysis,admission to ICU,and pulmonary infection being suspected source of bacteremia are independent risk factors for the prognosis of AB BSI in elderly patients.

7.
Food Sci Technol Int ; : 10820132231219859, 2023 Dec 20.
Artículo en Inglés | MEDLINE | ID: mdl-38115801

RESUMEN

Hyperuricemia, a condition characterized by elevated levels of uric acid in the blood, is known as a risk factor for gout disease. In this study, we isolated a total of 72 MRS-grown colonies and evaluated their purine nucleosidase (PNase) activity. Among the isolated bacteria, Levilactobacillus (L.) brevis LAB42 displayed the highest PNase activity. Our findings also indicate that PNase activity can vary among lactic acid bacterial strains and during different growth phases. Based on the kinetics study, LAB42 consistently exhibits the highest PNase activity. Due to its ability to attach to Caco-2 cells and its resistance to acidic environments and bile exposure, L. brevis LAB42 was chosen for further studies and showed that with the right combination of additives, it has the potential to be an appropriate starter for milk fermentation.

8.
Int J Mol Sci ; 24(22)2023 Nov 14.
Artículo en Inglés | MEDLINE | ID: mdl-38003501

RESUMEN

The pentatricopeptide repeat (PPR) gene family is one of the largest gene families in land plants. However, current knowledge about the evolution of the PPR gene family remains largely limited. In this study, we performed a comparative genomic analysis of the PPR gene family in O. sativa and its wild progenitor, O. rufipogon, and outlined a comprehensive landscape of gene duplications. Our findings suggest that the majority of PPR genes originated from dispersed duplications. Although segmental duplications have only expanded approximately 11.30% and 13.57% of the PPR gene families in the O. sativa and O. rufipogon genomes, we interestingly obtained evidence that segmental duplication promotes the structural diversity of PPR genes through incomplete gene duplications. In the O. sativa and O. rufipogon genomes, 10 (~33.33%) and 22 pairs of gene duplications (~45.83%) had non-PPR paralogous genes through incomplete gene duplication. Segmental duplications leading to incomplete gene duplications might result in the acquisition of domains, thus promoting functional innovation and structural diversification of PPR genes. This study offers a unique perspective on the evolution of PPR gene structures and underscores the potential role of segmental duplications in PPR gene structural diversity.


Asunto(s)
Duplicación de Gen , Oryza , Oryza/genética , Genes de Plantas , Genómica , Filogenia , Evolución Molecular
9.
Foods ; 12(19)2023 Oct 09.
Artículo en Inglés | MEDLINE | ID: mdl-37835356

RESUMEN

Traditional fermented milk from the western Sichuan plateau of China has a unique flavor and rich microbial diversity. This study explored the quality formation mechanism in fermented milk inoculated with Lactobacillus brevis NZ4 and Kluyveromyces marxianus SY11 (MFM), the dominant microorganisms isolated from traditional dairy products in western nan. The results indicated that MFM displayed better overall quality than the milk fermented with L. brevis NZ4 (LFM) and K. marxianus SY11 (KFM), respectively. MFM exhibited good sensory quality, more organic acid types, more free amino acids and esters, and moderate acidity and ethanol concentrations. Non-targeted metabolomics showed a total of 885 metabolites annotated in the samples, representing 204 differential metabolites between MFM and LFM and 163 between MFM and KFM. MFM displayed higher levels of N-acetyl-L-glutamic acid, cysteinyl serine, glaucarubin, and other substances. The differential metabolites were mainly enriched in pathways such as glycerophospholipid metabolism, arginine biosynthesis, and beta-alanine metabolism. This study speculated that L. brevis affected K. marxianus growth via its metabolites, while the mixed fermentation of these strains significantly changed the metabolism pathway of flavor-related substances, especially glycerophospholipid metabolism. Furthermore, mixed fermentation modified the flavor and quality of fermented milk by affecting cell growth and metabolic pathways.

10.
Dalton Trans ; 52(33): 11691-11697, 2023 Aug 22.
Artículo en Inglés | MEDLINE | ID: mdl-37552532

RESUMEN

The performance of organic light emitting devices (OLEDs) fabricated using Ir(III) complexes bearing 1,3,4-oxadiazole (OXD)-based cyclometallic ligands still needs to be improved. In this work, Ir3+ was coordinated with a 2-(9,9-diethyl-9H-fluoren-2-yl)-1,3,4-oxadiazole (F-OXD) fragment, which was modified with various functionalized substituents, including fluorenyl, OXD and carbazolyl groups. Three complexes, named Ir-Flu, Ir-OXD and Ir-Cz, were synthesized successfully and their photophysical, electrochemical and electroluminescence properties were investigated in detail. All these complexes exhibited yellow-orange emission in solution and a distinct aggregation-induced phosphorescent emission (AIPE) phenomenon was observed. Monochrome OLEDs were fabricated using these phosphorescent dopants, and the turn-on voltage (V), luminance (L) and current efficiency (CE) showed significant improvement compared to analogous OXD-based Ir(III) complexes reported before. In particular, the device with Ir-OXD as the dopant achieved the highest maximum brightness of 25 014 cd m-2 and the lowest efficiency roll-off (42.6%) at the maximum luminance among all the devices. These results provided a proven strategy of functionalized decoration of OXD-based complexes to achieve superior luminous efficiency devices.

11.
Ultrasonics ; 128: 106883, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36402115

RESUMEN

Corrosion damage in reinforcing steel bars has been a major cause of cracking and spalling of reinforced concrete. To extend the service life of concrete structures, non-destructive testing methods are necessary to assess the corrosion status in order to conduct a timely repair. At the early stage of corrosion, rust grows from the reinforcing bar, subsequently generates cracks towards the surface of the concrete. Ultrasonic methods have been widely used to detect cracks in concrete. However, it is challenging to characterise them due to the heterogeneous material properties of the concrete. In this paper, ultrasonic imaging technique based on diffuse coda wave has been explored to inspect and characterise corrosion-induced cracks. In this method, scattering cross-section of the crack is reconstructed with the Locadiff imaging technique. Based on the assumption that both crack tips have the same scattering cross-section, the size of the crack can be estimated when the location of the reinforcing bar is known. Numerical simulations were carried out to image straight and curved cracks, showing excellent accuracy. Experiments were designed subsequently on concrete samples with accelerated corrosion. The induced cracks were characterised by the proposed ultrasonic method, and compared with X-ray CT results, showing very good agreement.

12.
Microbiol Spectr ; 10(6): e0258022, 2022 12 21.
Artículo en Inglés | MEDLINE | ID: mdl-36314973

RESUMEN

The rapid and accurate identification of the causing agents during bacterial infections would greatly improve pathogen transmission, prevention, patient care, and medical treatments in clinical settings. Although many conventional and molecular methods have been proven to be efficient and reliable, some of them suffer technical biases and limitations that require the development and application of novel and advanced techniques. Recently, due to its cost affordability, noninvasiveness, and label-free feature, Raman spectroscopy (RS) is emerging as a potential technique for fast bacterial detection. However, the method is still hampered by many technical issues, such as low signal intensity, poor reproducibility, and standard data set insufficiency, among others. Thus, it should be cautiously claimed that Raman spectroscopy could provide practical applications in real-world settings. In order to evaluate the implementation potentials of Raman spectroscopy in the identification of bacterial pathogens, we investigated 30 bacterial species belonging to 9 different bacterial genera that were isolated from clinical samples via surfaced enhanced Raman spectroscopy (SERS). A total of 17,149 SERS spectra were harvested from a Raman spectrometer and were further analyzed via machine learning approaches, which showed that a convolutional neural network (CNN) deep learning algorithm achieved the highest prediction accuracy for recognizing pathogenic bacteria at both the genus and species levels. In summary, the SERS technique holds a promising potential for fast bacterial pathogen identification in clinical laboratories with the integration of machine learning algorithms, which might be further developed and sharpened for the direct identification and prediction of bacterial pathogens from clinical samples. IMPORTANCE In this study, we investigated 30 bacterial species belonging to 9 different bacterial genera that were isolated from clinical samples via surfaced enhanced Raman spectroscopy (SERS). A total of 17,149 SERS spectra were harvested from a Raman spectrometer and were further analyzed via machine learning approaches, the results of which showed that the convolutional neural network (CNN) deep learning algorithm could achieve the highest prediction accuracy for recognizing pathogenic bacteria at both the genus and species levels. Taken together, we concluded that the SERS technique held a promising potential for fast bacterial pathogen diagnosis in clinical laboratories with the integration of deep learning algorithms, which might be further developed and sharpened for the direct identification and prediction of bacterial pathogens from clinical samples.


Asunto(s)
Infecciones Bacterianas , Aprendizaje Profundo , Humanos , Espectrometría Raman/métodos , Reproducibilidad de los Resultados , Bacterias , Infecciones Bacterianas/diagnóstico
13.
Front Cardiovasc Med ; 9: 844733, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35463787

RESUMEN

Background: Whether heart failure (HF) is an independent risk factor of ischemic stroke (IS) and hemorrhagic stroke remains controversial. We employed a multivariable Mendelian randomization (MR) to further investigate the causal effects of HF on the risk of stroke and stroke subtypes. Methods: Genetically predicted HF was selected as an instrumental variable (IV) from published genome-wide association studies (GWAS) meta-analyses. Stroke data with different etiologies were extracted as outcome variables from another two GWAS meta-analyses. The random-effects inverse variance-weighted (IVW) model was applied as the main method, along with sensitivity analysis. Atrial fibrillation (AF), coronary heart disease (CHD), and systolic blood pressure (SBP) were controlled for mediating effects in multivariable MR. Results: Genetically predicted HF was significantly associated with any IS [odds ratio (OR), 1.39; 95% CI, 1.12-1.74; p = 0.03], large artery stroke (LAS; OR, 1.84; 95% CI, 1.27-2.65; p = 0.001), and cardioembolic stroke (CES; OR, 1.73; 95% CI, 1.21-2.47; p = 0.003), but without small vessel stroke (SVS; OR, 1.1; 95% CI, 0.80-1.52; p = 0.56) and intracerebral hemorrhage (ICH; OR, 0.86; 95% CI, 0.41-1.83; p = 0.699) in univariable MR. However, these significant associations were attenuated to the null after adjusting for confounding factor in multivariable MR. Conclusion: There was no direct causal association between HF and stroke in our study. The association between HF and IS can be driven by AF, CHD, and SBP.

14.
Viruses ; 14(3)2022 02 26.
Artículo en Inglés | MEDLINE | ID: mdl-35336890

RESUMEN

Bacillus subtilis is an important bacterial species due to its various industrial, medicinal, and agricultural applications. Prophages are known to play vital roles in host properties. Nevertheless, studies on the prophages and temperate phages of B. subtilis are relatively limited. In the present study, an in silico analysis was carried out in sequenced B. subtilis strains to investigate their prevalence, diversity, insertion sites, and potential roles. In addition, the potential for UV induction and prevalence was investigated. The in silico prophage analysis of 164 genomes of B. subtilis strains revealed that 75.00% of them contained intact prophages that exist as integrated and/or plasmid forms. Comparative genomics revealed the rich diversity of the prophages distributed in 13 main clusters and four distinct singletons. The analysis of the putative prophage proteins indicated the involvement of prophages in encoding the proteins linked to the immunity, bacteriocin production, sporulation, arsenate, and arsenite resistance of the host, enhancing its adaptability to diverse environments. An induction study in 91 B. subtilis collections demonstrated that UV-light treatment was instrumental in producing infective phages in 18.68% of them, showing a wide range of host specificity. The high prevalence and inducibility potential of the prophages observed in this study implies that prophages may play vital roles in the B. subtilis host.


Asunto(s)
Bacteriófagos , Profagos , Bacillus subtilis/genética , Bacteriófagos/genética , Genoma Viral , Plásmidos , Prevalencia , Profagos/genética
15.
Huan Jing Ke Xue ; 43(1): 550-559, 2022 Jan 08.
Artículo en Chino | MEDLINE | ID: mdl-34989540

RESUMEN

Clarifying the characteristics of soil microbial nutrient limitation and its driving mechanisms during vegetation restoration after farmland abandonment has important implications for revealing soil nutrient cycling and maintaining ecosystem stability. To determine the limitation of soil microbial nutrients and its relationship with soil properties along a chronosequence of abandoned farmland in the middle of the Qinling Mountains, the soil physicochemical properties and five enzyme activities (ß-1,4-glucosidase (BG), cellobiohydrolase (CBH), ß-1,4-N-acetylglucosaminidase (NAG), leucine aminopeptidase (LAP), and acid phosphatase (AP)) were measured, and models of extracellular enzymatic activity were applied. The results showed that the activities of BG, CBH, NAG, LAP, and AP were significantly increased following farmland abandonment. With the increasing years of abandonment, the ratios of (BG+CBH)/(NAG+LAP) and (BG+CBH)/AP significantly decreased, whereas the ratio of (NAG+LAP)/AP increased. Correlation analysis showed that most soil physicochemical properties were significantly correlated with extracellular enzyme activities and extracellular enzymatic stoichiometry. The vector length of extracellular enzymatic stoichiometry decreased with the increase in abandonment years, indicating that the limitation of soil microorganisms on carbon (C) was reduced. Moreover, the vector angles (>45°) showed a decreasing trend, indicating that microbial metabolisms were limited by phosphorus (P) and gradually decreased. Regression analysis showed that the C and P limitations were significantly related to total nutrients, available nutrients, nutrient ratio, and soil physical properties. Partial least squares path modeling (PLS-PM) revealed that the C and P limitations were directly regulated by nutrient ratio. PLS-PM further showed that soil total nutrients indirectly affected soil microbial C and P limitations by affecting nutrient ratio, and nutrient ratio affected the soil metabolism limitation via available nutrients and pH. Our study suggests that the characteristics of microbial metabolism during the vegetation restoration process reflect the mechanism of microorganism-mediated soil nutrient cycling, which provides a theoretical basis for revealing the community dynamics and stability during the vegetation restoration process and maintaining the regional ecological environment security in the Qinling Mountains.


Asunto(s)
Ecosistema , Microbiología del Suelo , Carbono/análisis , China , Nitrógeno/análisis , Fósforo/análisis , Suelo
17.
BMC Neurol ; 21(1): 8, 2021 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-33407227

RESUMEN

BACKGROUND: Clinically, bromadiolone poisoning is characterized by severe bleeding complications in various organs and tissues. Bromadiolone-induced toxic encephalopathy is extremely rare. Here, we report a special case of bromadiolone-induced reversible toxic encephalopathy in a patient who had symmetrical lesions in the deep white matter. CASE PRESENTATION: A 23-year-old woman mainly presented with dizziness, fatigue, alalia and unsteady gait after the ingestion of bromadiolone. The laboratory examinations showed normal coagulation levels. Brain magnetic resonance imaging (MRI) showed apparent diffusion restriction in the bilateral deep white matter. The clinical manifestations and MRI alterations were reversible within one month of treatment with vitamin K. The neuropsychological assessment showed no neurodegenerative changes at the 2-year follow-up. CONCLUSION: With the increased use of bromadiolone as a rodenticide, more cases of ingestion have been reported annually over the past several years. Bromadiolone-induced toxic encephalopathy has no special clinical manifestations and is potentially reversible with timely treatment. Because of the reversible restricted diffusion on diffusion-weighted images (DWI) and low apparent diffusion coefficient (ADC) values, transient intramyelinic cytotoxic oedema is thought to be the cause rather than persistent ischaemia. The underlying pathophysiological mechanism is still unknown and may be coagulant-independent. This clinical case extends the current knowledge about neurotoxicity in cases of bromadiolone poisoning and indicates that MRI is useful for the early detection of bromadiolone-induced toxic encephalopathy.


Asunto(s)
4-Hidroxicumarinas/envenenamiento , Encéfalo/patología , Síndromes de Neurotoxicidad/etiología , Síndromes de Neurotoxicidad/patología , Rodenticidas/envenenamiento , Antifibrinolíticos/uso terapéutico , Encéfalo/efectos de los fármacos , Imagen de Difusión por Resonancia Magnética/métodos , Femenino , Humanos , Síndromes de Neurotoxicidad/tratamiento farmacológico , Intento de Suicidio , Vitamina K 1/uso terapéutico , Adulto Joven
18.
Polymers (Basel) ; 12(10)2020 Oct 02.
Artículo en Inglés | MEDLINE | ID: mdl-33023192

RESUMEN

Material-scale tests involving milligrams of samples are used to optimize fire-retardant coating formulations, but actual applications of these coatings require them to be assessed with structural-scale fire tests. This significant difference in the scale of testing (milligrams to kilograms of sample) raises many questions on the relations between the inherent flammability and thermal characteristics of the coating materials and their "performance" at the structural scale. Moreover, the expected "performance" requirements and the definition of "performance" varies at different scales. In this regard, the pathway is not established when designing and formulating fire-retardant coatings for structural steel sections or members. This manuscript explores the fundamental relationships across different scales of testing with the help of a fire-protective system based on acrylic resin with a typical combination of intumescent additives, viz. ammonium polyphosphate, pentaerythritol, and expandable graphite. One of the main outcomes of this work dictates that higher heat release rate values and larger amounts of material participating in the pyrolysis process per unit time will result in a rapid rise in steel substrate temperature. This information is very useful in the design and development of generic fire-retardant coatings.

19.
J Microbiol Biotechnol ; 30(12): 1862-1869, 2020 Dec 28.
Artículo en Inglés | MEDLINE | ID: mdl-32958736

RESUMEN

The spread of plasmid-mediated colistin resistance has posed a serious threat to public health owing to its effects on the emergence of pandrug-resistant bacteria. In this study, we investigated the prevalence and characteristics of mcr-1-positive Escherichia coli isolated from retail meat samples in Korea. In total, 1,205 E. coli strains were isolated from 3,234 retail meat samples in Korea. All E. coli strains were subjected to antimicrobial susceptibility testing and were examined for the presence of mcr-1 gene. All mcr-1-positive E. coli (n = 10, 0.8%) from retail meat were subjected to pulse-field gel electrophoresis (PFGE) and whole-genome sequencing (WGS). The transferability of mcr-1 gene was determined by conjugation assays. The mcr-1-positive strains exhibited diverse clonal types. Our mcr-1 genes were located in plasmids belonged to the IncI2 (n = 1) and IncX4 (n = 8) types, which were reported to be prevalent in Asia and worldwide, respectively. Most mcr-1 genes from mcr-1-positive strains (9/10) were transferable to the recipient strain and the transfer frequencies ranged from 2.4 × 10-3 to 9.8 × 10-6. Our data suggest that the specific types of plasmid may play an important role in spreading plasmid-mediated colistin resistance in Korea. Furthermore, our findings suggest that the retail meat may be an important tool for disseminating plasmid-mediated colistin resistance.


Asunto(s)
Proteínas de Escherichia coli/genética , Escherichia coli/genética , Carne/microbiología , Animales , Farmacorresistencia Bacteriana/genética , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/microbiología , Microbiología de Alimentos , Plásmidos , Prevalencia , República de Corea , Secuenciación Completa del Genoma
20.
Oncol Lett ; 20(3): 3035-3045, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32782621

RESUMEN

The aims of the present study were to investigate the clinical outcomes and safety of apatinib monotherapy in the treatment of patients with advanced epithelial ovarian carcinoma (EOC) who have progressed after standard regimens, and to analyze the vascular endothelial growth factor receptor 2 (VEGFR2) rs2071559 polymorphism. A total of 118 patients with advanced EOC who received apatinib treatment were included in the study. Tumor response was evaluated using progression-free survival (PFS) and overall survival (OS) time, and safety data were documented. Additionally, peripheral blood and peripheral blood mononuclear cell (PBMC) specimens from the patients with EOC were collected to perform the genotyping of genetic polymorphism and assess the mRNA expression of VEGFR2, respectively. The objective response rate across the 118 patients with advanced EOC was 38.98%, the disease control rate was 63.56%, the median PFS time was 4.65 months and the median OS time was 15.10 months. Regarding the polymorphism analysis, the prevalence of rs2071559 in VEGFR2 among the 118 patients with advanced EOC was recorded as the TT genotype in 72 cases (61.02%), TC genotype in 41 cases (34.75%) and CC genotype in 5 cases (4.23%), and the minor allele frequency of rs2071559 was 0.22. The distribution of the three genotypes was in accordance with the Hardy-Weinberg equilibrium (P=0.781). TC and CC genotypes were merged in the subsequent analysis. The prognosis analyses suggested that the median PFS time of patients with the TC/CC genotype and the TT genotype was 3.10 and 5.40 months, respectively (P=0.015). Moreover, the median OS time of the two genotypes was 12.60 and 17.50 months, respectively (P=0.009). However, no association was noted between genotype status of the polymorphism and adverse reactions. Additionally, the mRNA expression analysis indicated that the mRNA expression levels of VEGFR2 in PBMC specimens were significantly different between TT and TC/CC genotypes (P<0.001). The present study suggested that the clinical outcomes of patients with advanced EOC, who progressed after standard regimens and received apatinib treatment, might be influenced by the VEGFR2 rs2071559 polymorphism.

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