The inhibitory activity of a new scorpion venom-derived antimicrobial peptide Hp1470 against Gram-positive bacteria.

Antimicrobial peptides (AMPs) are a new type of antibiotic and target a variety of microbes, including antibiotic-resistant strains; thus, AMPs have attracted widespread interest. Scorpion venoms contain many bioactive peptides, including AMPs, and have become an important natural resource of peptide-based drugs. Here, the antibacterial peptide gene Hp1470 from the venom of the scorpion Heterometrus petersii was characterized, and its antibacterial activity was determined. The cDNA sequence of Hp1470 is 300 nt in length and contains an open reading frame (ORF) of 207 nt. The ORF was shown to encode 68 amino acid residues, including a signal peptide (23 aa), a mature peptide (13 aa), a C-terminal posttranslational processing signal (3 aa), and a propeptide (29 aa). Multiple sequence alignment results indicated that Hp1470 is an antibacterial peptide. The mature peptide Hp1470, which has a molecular mass of 1564.09 Da, was further chemically synthesized with a purity of greater than 95%. Antimicrobial assays showed that the synthesized Hp1470 exerted an inhibitory effect on Gram-positive bacteria and clinical drug-resistant strains, including PRSA and MRSA, but not Gram-negative bacteria. Hp1470 was further found to protect mice from MRSA infection, suggesting its potential application as an in vivo antimicrobial agent. Interestingly, Hp1470 only inhibited bacterial growth but did not kill bacteria, which was consistent with scanning electron microscopy results showing that Hp1470 did not lyse the cell membrane of Staphylococcus aureus. Our work provides a new direction for developing antibacterial agents with different modes of action from natural scorpion venoms.

Dynamic synthesis and transport of fluorescent substances from moulting scorpions.

Scorpion fluorescence under ultraviolet light is a well-known phenomenon, and its change is also a known biological feature during the scorpion moulting process. However, the synthesis and transport of fluorescent substances during the moulting stage remain unclear. In this study, in-depth investigations on the global fluorescence changes from the exoskeleton, fluorescence layer, coelomic fluid, and abdomen to the digestive glands indicated that the digestive glands, which occupy most of the space in the abdomen of the scorpion mesosoma segment, were responsible for synthesizing the fluorescent substances. More importantly, these fluorescent substances were produced in advance, before the moulting process, which contributed to the recovery of the fluorescent exoskeleton as early as possible. The synthesized fluorescent substances first entered the coelomic fluid, then successively passed through the inherent epithelial cell layer and two new formed endocuticle and exocuticle layers, and ultimately reached and became enriched in the new formed fluorescent layer, which was protected by the new epicuticle layer. These four new layers were the first to illustrate the structural features of the fluorescent exoskeleton. Due to the very soft body and the inability of the newly moulted scorpion to resist attacks from the predator, this special synthesis and transport strategy of the fluorescent substances could guarantee the rapid formation of the integrated fluorescent exoskeleton during the 24 h after ecdysis, which would be a novel biological feature during the scorpion evolution.