RESUMEN
OBJECTIVE: To explore the existence of natural foci on Marmota himalayana plague in Sichuan province and to provide basis for prevention and control of the disease. METHODS: Both epidemiological investigation and laboratory tests were used to provide the host animal and fleas of the vectors with Yersinia pestis carriers. RESULTS: 30 species of animals were found to belong to 10 orders. Ochotona curzoniae and M. himalayana were the most common ones while 7 species of the fleas belonged to 7 genera and 3 families. M. himalayana was the main reservoirs while Callopsylla dolabris and Oropsylla silantiewi served as vectors. The 13 Y. pestis were identified from 43 Marmota samples. 8 samples were identified under IHA, with the highest titer of herding-dogs serum as 1:10,240. 19 samples were F1 antigen positive using RIHA and the highest titer of M. himalayana serum was 1:409,600. The major foci was 4545 km2, distributed at Dege county in Sichuan province. CONCLUSION: We have confirmed the existence of natural foci on M. himalayana plague in Sichuan province.
Asunto(s)
Reservorios de Enfermedades , Marmota/microbiología , Peste , Yersinia pestis/aislamiento & purificación , Animales , Proteínas Bacterianas/sangre , China , Vectores de Enfermedades , Perros , Pruebas de Hemaglutinación/veterinaria , Insectos Vectores , Siphonaptera/microbiología , Yersinia pestis/inmunologíaRESUMEN
A rapid method for the analysis of metallothioneins (MT) in foods by capillary zone electrophoresis(CZE) was developed. Two isomers of MT (MT1, MT2) in foods were separated and determined. After a series of optimization, the separation and determination of MT1 and MT2 were obtained within 10 min by using the phosphate buffer system consisting of 0.02 mol/L Na2HPO4 and 0.02 mol/L NaH2PO4 (pH 7.0), and UV detection at 200 nm. Under the optimal experimental conditions, the minimum detectable limit was 1 mg/L, and the added standard recoveries of MT1, MT2 in foods were found to be in the range of 82.0%-93.4%. The relative standard deviations (RSD) were found to be lower than 10%. Therefore, with this simple and rapid method, the contents of two isomers in foods can be determined by external standard method after sample pretreatment.
