Leveraging electrochemical sensors to improve efficiency of cancer detection.

Electrochemical biosensors have emerged as a promising technology for cancer detection due to their high sensitivity, rapid response, low cost, and capability for non-invasive detection. Recent advances in nanomaterials like nanoparticles, graphene, and nanowires have enhanced sensor performance to allow for cancer biomarker detection, like circulating tumor cells, nucleic acids, proteins and metabolites, at ultra-low concentrations. However, several challenges need to be addressed before electrochemical biosensors can be clinically implemented. These include improving sensor selectivity in complex biological media, device miniaturization for implantable applications, integration with data analytics, handling biomarker variability, and navigating regulatory approval. This editorial critically examines the prospects of electrochemical biosensors for efficient, low-cost and minimally invasive cancer screening. We discuss recent developments in nanotechnology, microfabrication, electronics integration, multiplexing, and machine learning that can help realize the potential of these sensors. However, significant interdisciplinary efforts among researchers, clinicians, regulators and the healthcare industry are still needed to tackle limitations in selectivity, size constraints, data interpretation, biomarker validation, toxicity and commercial translation. With committed resources and pragmatic strategies, electrochemical biosensors could enable routine early cancer detection and dramatically reduce the global cancer burden.

Innovations in WO3 gas sensors: Nanostructure engineering, functionalization, and future perspectives.

This review critically examines the progress and challenges in the field of nanostructured tungsten oxide (WO3) gas sensors. It delves into the significant advancements achieved through nanostructuring and composite formation of WO3, which have markedly improved sensor sensitivity for gases like NO2, NH3, and VOCs, achieving detection limits in the ppb range. The review systematically explores various innovative approaches, such as doping WO3 with transition metals, creating heterojunctions with materials like CuO and graphene, and employing machine learning models to optimize sensor configurations. The challenges facing WO3 sensors are also thoroughly examined. Key issues include cross-sensitivity to different gases, particularly at higher temperatures, and long-term stability affected by factors like grain growth and volatility of dopants. The review assesses potential solutions to these challenges, including statistical analysis of sensor arrays, surface functionalization, and the use of novel nanostructures for enhanced performance and selectivity. In addition, the review discusses the impact of ambient humidity on sensor performance and the current strategies to mitigate it, such as composite materials with humidity shielding effects and surface functionalization with hydrophobic groups. The need for high operating temperatures, leading to higher power consumption, is also addressed, along with possible solutions like the use of advanced materials and new transduction principles to lower temperature requirements. The review concludes by highlighting the necessity for a multidisciplinary approach in future research. This approach should combine materials synthesis, device engineering, and data science to develop the next generation of WO3 sensors with enhanced sensitivity, ultrafast response rates, and improved portability. The integration of machine learning and IoT connectivity is posited as a key driver for new applications in areas like personal exposure monitoring, wearable diagnostics, and smart city networks, underlining WO3's potential as a robust gas sensing material in future technological advancements.

Advancements in wearable technology for monitoring lactate levels using lactate oxidase enzyme and free enzyme as analytical approaches: A review.

Lactate is a metabolite that holds significant importance in human healthcare, biotechnology, and the food industry. The need for lactate monitoring has led to the development of various devices for measuring lactate concentration. Traditional laboratory methods, which involve extracting blood samples through invasive techniques such as needles, are costly, time-consuming, and require in-person sampling. To overcome these limitations, new technologies for lactate monitoring have emerged. Wearable biosensors are a promising approach that offers non-invasiveness, low cost, and short response times. They can be easily attached to the skin and provide continuous monitoring. In this review, we evaluate different types of wearable biosensors for lactate monitoring using lactate oxidase enzyme as biological recognition element and free enzyme systems.

Plasmonic Nanoparticle-Enhanced Optical Techniques for Cancer Biomarker Sensing.

This review summarizes recent advances in leveraging localized surface plasmon resonance (LSPR) nanotechnology for sensitive cancer biomarker detection. LSPR arising from noble metal nanoparticles under light excitation enables the enhancement of various optical techniques, including surface-enhanced Raman spectroscopy (SERS), dark-field microscopy (DFM), photothermal imaging, and photoacoustic imaging. Nanoparticle engineering strategies are discussed to optimize LSPR for maximum signal amplification. SERS utilizes electromagnetic enhancement from plasmonic nanostructures to boost inherently weak Raman signals, enabling single-molecule sensitivity for detecting proteins, nucleic acids, and exosomes. DFM visualizes LSPR nanoparticles based on scattered light color, allowing for the ultrasensitive detection of cancer cells, microRNAs, and proteins. Photothermal imaging employs LSPR nanoparticles as contrast agents that convert light to heat, producing thermal images that highlight cancerous tissues. Photoacoustic imaging detects ultrasonic waves generated by LSPR nanoparticle photothermal expansion for deep-tissue imaging. The multiplexing capabilities of LSPR techniques and integration with microfluidics and point-of-care devices are reviewed. Remaining challenges, such as toxicity, standardization, and clinical sample analysis, are examined. Overall, LSPR nanotechnology shows tremendous potential for advancing cancer screening, diagnosis, and treatment monitoring through the integration of nanoparticle engineering, optical techniques, and microscale device platforms.

A DNA biosensor strategy in monitoring of Vinorelbine breast cancer drug using catalytic effect of Pt-Pd-ZnO/SWCNTs.

The present research work introduced a new electrocatalyst (Pt-Pd-ZnO/SWCNTs in this case) to the fabrication of a powerful DNA biosensor in the monitoring of Vinorelbine anticancer drug. The characterization information confirms the high purity of Pt-Pd-ZnO/SWCNTs nanocomposite and an intercalation reaction between Vinorelbine anticancer drug and the guanine base of DNA in an aqueous solution. The reducing signal of DNA after interaction with Vinorelbine drug showed a linear analytical range of 0.1-120 µM with a detection limit of 0.05 µM. The biosensor was fabricated by layer-by-layer modification of glassy carbon electrode with ds-DNA and Pt-Pd-ZnO/SWCNTs nanocomposite and used as the working electrode to sensing of vinorelbine drug in pharmaceutical and other real samples with acceptable recovery data. The preferential intercalation mode for the binding of vinorelbine anticancer drug into the ds-DNA receptor is clarified using the molecular docking study.

An Overview to Molecularly Imprinted Electrochemical Sensors for the Detection of Bisphenol A.

Bisphenol A (BPA) is an industrial chemical used extensively in plastics and resins. However, its endocrine-disrupting properties pose risks to human health and the environment. Thus, accurate and rapid detection of BPA is crucial for exposure monitoring and risk mitigation. Molecularly imprinted electrochemical sensors (MIES) have emerged as a promising tool for BPA detection due to their high selectivity, sensitivity, affordability, and portability. This review provides a comprehensive overview of recent advances in MIES for BPA detection. We discuss the operating principles, fabrication strategies, materials, and methods used in MIES. Key findings show that MIES demonstrate detection limits comparable or superior to conventional methods like HPLC and GC-MS. Selectivity studies reveal excellent discrimination between BPA and structural analogs. Recent innovations in nanomaterials, novel monomers, and fabrication techniques have enhanced sensitivity, selectivity, and stability. However, limitations exist in reproducibility, selectivity, and stability. While challenges remain, MIES provide a low-cost portable detection method suitable for on-site BPA monitoring in diverse sectors. Further optimization of sensor fabrication and characterization will enable the immense potential of MIES for field-based BPA detection.

Revolutionizing cancer monitoring with carbon-based electrochemical biosensors.

Cancer monitoring plays a critical role in improving patient outcomes by providing early detection, personalized treatment options, and treatment response tracking. Carbon-based electrochemical biosensors have emerged in recent years as a revolutionary technology with the potential to revolutionize cancer monitoring. These sensors are useful for clinical applications because of their high sensitivity, selectivity, rapid response, and compatibility with miniaturized equipment. This review paper gives an in-depth look at the latest developments and the possibilities of carbon-based electrochemical sensors in cancer surveillance. The essential principles of carbon-based electrochemical sensors are discussed, including their structure, operating mechanisms, and critical qualities that make them suited for cancer surveillance. Furthermore, we investigate their applicability in detecting specific cancer biomarkers, evaluating therapy responses, and detecting cancer recurrence early. Additionally, a comparison of carbon-based electrochemical sensor performance measures, including sensitivity, selectivity, accuracy, and limit of detection, is presented in contrast to existing monitoring methods and upcoming technologies. Finally, we discuss prospective tactics, future initiatives, and commercialization opportunities for improving the capabilities of these sensors and integrating them into normal clinical practice. The review highlights the potential impact of carbon-based electrochemical sensors on cancer diagnosis, treatment, and patient outcomes, as well as the importance of ongoing research, collaboration, and validation studies to fully realize their potential in revolutionizing cancer monitoring.

Strategies and Applications of Graphene and Its Derivatives-Based Electrochemical Sensors in Cancer Diagnosis.

Graphene is an emerging nanomaterial increasingly being used in electrochemical biosensing applications owing to its high surface area, excellent conductivity, ease of functionalization, and superior electrocatalytic properties compared to other carbon-based electrodes and nanomaterials, enabling faster electron transfer kinetics and higher sensitivity. Graphene electrochemical biosensors may have the potential to enable the rapid, sensitive, and low-cost detection of cancer biomarkers. This paper reviews early-stage research and proof-of-concept studies on the development of graphene electrochemical biosensors for potential future cancer diagnostic applications. Various graphene synthesis methods are outlined along with common functionalization approaches using polymers, biomolecules, nanomaterials, and synthetic chemistry to facilitate the immobilization of recognition elements and improve performance. Major sensor configurations including graphene field-effect transistors, graphene modified electrodes and nanocomposites, and 3D graphene networks are highlighted along with their principles of operation, advantages, and biosensing capabilities. Strategies for the immobilization of biorecognition elements like antibodies, aptamers, peptides, and DNA/RNA probes onto graphene platforms to impart target specificity are summarized. The use of nanomaterial labels, hybrid nanocomposites with graphene, and chemical modification for signal enhancement are also discussed. Examples are provided to illustrate applications for the sensitive electrochemical detection of a broad range of cancer biomarkers including proteins, circulating tumor cells, DNA mutations, non-coding RNAs like miRNA, metabolites, and glycoproteins. Current challenges and future opportunities are elucidated to guide ongoing efforts towards transitioning graphene biosensors from promising research lab tools into mainstream clinical practice. Continued research addressing issues with reproducibility, stability, selectivity, integration, clinical validation, and regulatory approval could enable wider adoption. Overall, graphene electrochemical biosensors present powerful and versatile platforms for cancer diagnosis at the point of care.

Natural waste-derived nano photocatalysts for azo dye degradation.

Due to their widespread application in water purification, there is a significant interest in synthesising nanoscale photocatalysts. Nanophotocatalysts are primarily manufactured through chemical methods, which can lead to side effects like pollution, high-energy usage, and even health issues. To address these issues, "green synthesis" was developed, which involves using plant extracts as reductants or capping agents rather than industrial chemical agents. Green fabrication has the benefits of costs less, pollution reduction, environmental protection and human health safety, compared to the traditional methods. This article summarises recent advances in the environmentally friendly synthesis of various nanophotocatalysts employed in the degradation of azo dyes. This study compiles critical findings on natural and artificial methods to achieve the goal. Green synthesis is constrained by the time and place of production and issues with low purity and poor yield, reflecting the complexity of plants' geographical and seasonal distributions and their compositions. However, green photocatalyst synthesis provides additional growth opportunities and potential uses.

Smart and sensitive nanomaterial-based electrochemical sensor for the determination of a poly (ADP-ribose) polymerase (PARP) inhibitor anticancer agent.

In this research, we propose a novel approach for constructing a sensitive and selective electrochemical sensor utilizing high-quality multi-walled carbon nanotubes functionalized with amino groups (MWCNT-NH2) for the detection of Talazoparib (TLZ), a poly (ADP-ribose) polymerase (PARP) enzyme inhibitor, in real samples. The MWCNT-NH2-based sensor exhibited remarkable performance characteristics, including excellent repeatability, reproducibility, and high selectivity against various interferences. Under optimized conditions, the sensor demonstrated a wide linear concentration range of 1.0-5.0 µM, with a low limit of detection (LOD) of 0.201 µM. Substantiated by rigorous analysis of pharmaceutical and biological matrices, our methodology emerges as a paragon of reliability, boasting recovery rates within the satisfactory bracket of 96.38-105.25%. The successful application of the MWCNT-NH2-based sensor in practical sample analysis highlights its potential for implementation in clinical and pharmaceutical settings. This research not only advances the application of MWCNT-NH2 in electrochemical sensing but also opens new avenues for the development and monitoring of innovative anticancer treatments. The insights gained from our study have far-reaching implications, pointing toward a future where precision and innovation converge to improve patient care and treatment outcomes.

Nanodiamond (ND)-Based ND@CuAl2O4@Fe3O4 electrochemical sensor for Tofacitinib detection: A unified approach to integrate experimental data with DFT and molecular docking.

Tofacitinib (TOF) is gaining recognition as a potent therapeutic agent for a variety of autoimmune disorders, including rheumatoid arthritis and psoriasis. Ensuring precise drug concentration control during treatment necessitates a rapid and sensitive detection method. This study introduces a novel electrochemical sensor employing a composite of nanodiamond (ND), copper aluminate spinel oxide (CuAl2O4), and iron (II, III) oxide (Fe3O4) as modified materials for efficient TOF detection. Extensive analyses using physicochemical and electrochemical techniques were carried out to characterize the morphological, structural, and electrochemical properties of the ND@CuAl2O4@Fe3O4 composite. Thereafter, various voltammetric methods were utilized to evaluate the electrochemical behavior of the ND@CuAl2O4@Fe3O4-modified glassy carbon electrode (GCE) concerning TOF determination. The fabricated electrode showcased superior performance in electrochemical TOF detection in a buffered solution (pH = 5), achieving a remarkably low detection limit of 7.8 nM and a linear response from 0.05 µM to 13.21 µM. Furthermore, applying the modified electrode as an electrochemical sensor exhibited exceptional selectivity, stability, and practicality in determining TOF in pharmaceutical and biological samples. Alongside the sensor development, this study conducted a thorough investigation using Density Functional Theory (DFT) for the geometry optimization of TOF and the TOF-ND complex. Consequently performed molecular docking studies using Janus Kinase 1 (JAK1) (PDB ID: 3EYG) and JAK3 (PDB ID: 3LXK) indicated higher interaction of the TOF-ND conjugate with the JAKs, reflected by binding energies of -12.9 kcal/mol and -11.7 kcal/mol for JAK1 and JAK3 respectively, compared to -7.0 kcal/mol and -6.9 kcal/mol for TOF alone. These findings illustrate the potential of the ND-based ND@CuAl2O4@Fe3O4 composite as a proficient sensing material for TOF detection and the merits of DFT in providing a detailed understanding of the interactions at play. This pioneering research holds promise for real-time TOF monitoring, which will advance personalized treatment strategies and improve therapeutic outcomes for patients with autoimmune disorders.

Cu-BTC MOF/ionic liquid nanocomposite as novel catalyst to electrochemical monitoring of digoxin in pharmaceutical and environmental samples.

There is no effective environmental treatment strategy that does not include monitoring for pharmaceutical compounds in environmental and biological fluids. The widespread presence of pharmaceutical-based pollutants in water sources is a significant public health concern. The treatment process relies heavily on maintaining a stable digoxin concentration in bodily fluids. Finding the correct dose for this medication appears to be crucial. In this research, an easy and high sensibility electrochemical sensor was developed to determine digoxin based on a paste electrode (CPE) that was modified with Cu-BTC MOF and ion liquid ((IL); 1-Methyl-3-Butyl-imidazolinium bromide in this case) using voltammetric methods in 0.1 M phosphate buffer solution (PBS) at pH 5.0. The sensor's selectivity was significantly increased by using Cu-BTC MOF and IL to detect digoxin. The characteristics of the electrode modifiers were evaluated by SEM, XRD and EDS techniques. The LDR was found to be 0.1-40 µM and the LOD of 0.08 µM, respectively.

Traditional methods and biosensors for detecting disinfection by-products in water: A review.

In recent years, pollution caused by disinfection by-products (DBPs) has become a global concern. Initially, there were fewer contaminants, and the mechanism of their generation was unclear; however, the number of contaminants has increased exponentially as a result of rapid industrialization and numerous economic activities (e.q., during the outbreak of COVID-19 a surge in the use of chlorinated disinfectants was observed). DBP toxicity results in various adverse health effects and organ failure in humans. In addition, it profoundly affects other forms of life, including animals, plants, and microorganisms. This review comprehensively discusses the pre-treatment methods of traditional and emerging DBPs and the technologies applied for their detection. Additionally, this paper provides a detailed discussion of the principles, applicability, and characteristics of traditional large-scale instrumentation methods (such as gas/liquid/ion chromatography coupled with mass spectrometry) for detecting DBPs based on their respective detection techniques. At the same time, the design, functionality, classification, and characteristics of rapid detection technologies (such as biosensors) are also detailed and analyzed.

Unveiling the interactions between biomaterials and heterocyclic dyes: A sustainable approach for wastewater treatment.

The present Review investigates the interactions between biomaterials and heterocyclic dyes, focusing on their potential application in sustainable wastewater treatment. Heterocyclic dyes are widely used in various industries, resulting in their widespread presence in wastewater, posing environmental challenges. This review explores the utilization of biomaterials as adsorbents for the removal of heterocyclic dyes from contaminated water sources. The interactions between biomaterials, such as cellulose, microfibrilated cellulose and lignin and different heterocyclic dyes are examined through reported experimental analysis and characterization techniques. The study evaluates the adsorption capacity, kinetics, and thermodynamics of the biomaterial-dye systems to elucidate the underlying mechanisms and optimize the treatment process. The review highlight the promising potential of biomaterial-based approaches for sustainable wastewater treatment, providing insights for the development of efficient and environmentally friendly dye removal technologies.

Nanohybrid of antimonene@Ti3C2Tx-based electrochemical aptasensor for lead detection.

Lead ions (Pb2+), as one of many common heavy metallic environmental pollutants, can cause serious side-effects and result in chronic poisoning to people's health, so it is highly significant to monitor Pb2+ efficiently and sensitively. Here, we proposed an antimonene@Ti3C2Tx nanohybrid-based electrochemical aptamer sensor (aptasensor) for high sensitive Pb2+ determination. The sensing platform of nanohybrid was synthesized by ultrasonication, possessing the advantages of both antimonene and Ti3C2Tx, which not only can vastly enlarge the sensing signal of the proposed aptasensor, but also greatly simplified its manufacturing flow, because antimonene can strongly interact with aptamer through noncovalently bound. The surface morphology and microarchitecture of the nanohybrid were perused by several methods such as scanning electron microscope (SEM), energy-dispersive X-ray mapping spectroscopy (EDS), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and atomic force microscope (AFM). Under optimal empirical conditions, the proposed aptasensor exhibited a wide linear correlation of the current signals with the logarithm of CPb2+ (Log CPb2+) over the span from 1 × 10-12 to 1 × 10-7 M and provided a trace discernment limit of 3.3 × 10-13 M. Moreover, the constructed aptasensor displayed superior repeatability, great consistency, eminent selectivity, and beneficial reproducibility, implying its extreme potential application for water quality control and the environmental monitoring of Pb2+.

Gemcitabine drug intercalation with ds-DNA at surface of ds-DNA/Pt-ZnO/SWCNTs/GCE biosensor: A DNA-biosensor for gemcitabine monitoring confirmed by molecular docking study.

Herein, a facile and highly sensitive electroanalytical tool for monitoring and quantifying the antineoplastic drug gemcitabine in real sample was provided. In this regard, a novel DNA-biosensor based on Pt-doped ZnO decorated single walled carbon nanotubes (Pt-ZnO/SWCNTs) hybrid nanomaterial modification of glassy carbon electrode (GCE) was fabricated. Ds-DNA (Calf Thymus), as a biological recognition element, was decorated onto nanomaterial-modified GCE via layer-by-layer fabrication strategy to attain ultimate biosensor ds-DNA/Pt-ZnO/SWCNTs/GCE. The characterizations confirmed the successful fabrication of hybrid nanomaterial, as well as the modification of electrode surface by fabricated nanomaterial. The electrochemical impedance spectroscopy (EIS) analysis revealed that the nanomaterial modification of GCE surface enhanced the electrical conductivity thanks to the synergistic effects of Pt-ZnO and SWCNTs structures, thereby boosted the electrocatalytic activity of the resultant biosensor. The electrochemical characterization results showed that the suggested biosensor is capable of detecting gemcitabine in a wide concentration range of 0.01-30.0 µM, with a detection limit of 5.0 nM. The intercalation binding mode of Gemcitabine inside guanine and cytosine rich region of DNA receptor was approved by molecular docking study. The results of the experimental data were well congruent with the molecular docking analysis, which showed that the binding mode of gemcitabine drug with ds-DNA was intercalation.

Calf thymus ds-DNA intercalation with pendimethalin herbicide at the surface of ZIF-8/Co/rGO/C3N4/ds-DNA/SPCE; A bio-sensing approach for pendimethalin quantification confirmed by molecular docking study.

Pendimethalin (PND) is a herbicide that is regarded to be possibly carcinogenic to humans and toxic to the environment. Herein, we fabricated a highly sensitive DNA biosensor based on ZIF-8/Co/rGO/C3N4 nanohybrid modification of a screen-printed carbon electrode (SPCE) to monitor PND in real samples. The layer-by-layer fabrication pathway was conducted to construct ZIF-8/Co/rGO/C3N4/ds-DNA/SPCE biosensor. The physicochemical characterization techniques confirmed the successful synthesis of ZIF-8/Co/rGO/C3N4 hybrid nanocomposite, as well as the appropriate modification of the SPCE surface. The utilization of ZIF-8/Co/rGO/C3N4 nanohybrid as a modifier was analyzed using. The electrochemical impedance spectroscopy results showed that the modified SPCE exhibited significantly lowered charge transfer resistance due to the enhancement of its electrical conductivity and facilitation of the transfer of charged particles. The proposed biosensor successfully quantified PND in a wide concentration range of 0.01-35 µM, with a limit of detection (LOD) value of 8.0 nM. The PND monitoring capability of the fabricated biosensor in real samples including rice, wheat, tap, and river water samples was verified with a recovery range of 98.2-105.6%. Moreover, to predict the interaction sites of PND herbicide with DNA, the molecular docking study was performed between the PND molecule and two sequence DNA fragments and confirmed the experimental findings. This research sets the stage for developing highly sensitive DNA biosensors that will be used to monitor and quantify toxic herbicides in real samples by fusing the advantages of nanohybrid structures with crucial knowledge from a molecular docking investigation.

Application of zeolite based nanocomposites for wastewater remediation: Evaluating newer and environmentally benign approaches.

The presence of heavy metal ions and emerging pollutants in water poses a great risk to various biological ecosystems as a result of their high toxicity. Consequently, devising efficient and environmentally friendly methods to decontaminate these waters is of high interest to many researchers around the world. Among the varied water treatment and desalination means, adsorption and photocatalysis have been widely employed. However, the discussion and analysis of the use of zeolite-based composites as adsorbents are somehow minimal. The porous aluminosilicates (zeolites) are excellent candidates in wastewater treatment owing to various mechanisms of pollutants removal that they possess. The purpose of this review is thus to provide a synopsis of the current developments in the fabrication and application of nanocomposites based on zeolite as adsorbents and photocatalysts for the extraction of heavy metals, dyes and emerging pollutants from wastewaters. The review goes on to look into the effect of weight ratio on photocatalyst, photodegradation pathways, and various factors that influence photocatalysis and adsorption.