Carbon isotope trends across a century of herbarium specimens suggest CO2 fertilization of C4 grasses.

Increasing atmospheric CO2 is changing the dynamics of tropical savanna vegetation. C3 trees and grasses are known to experience CO2 fertilization, whereas responses to CO2 by C4 grasses are more ambiguous. Here, we sample stable carbon isotope trends in herbarium collections of South African C4 and C3 grasses to reconstruct 13C discrimination. We found that C3 grasses showed no trends in 13C discrimination over the past century but that C4 grasses increased their 13C discrimination through time, especially since 1950. These changes were most strongly linked to changes in atmospheric CO2 rather than to trends in rainfall climatology or temperature. Combined with previously published evidence that grass biomass has increased in C4-dominated savannas, these trends suggest that increasing water-use efficiency due to CO2 fertilization may be changing C4 plant-water relations. CO2 fertilization of C4 grasses may thus be a neglected pathway for anthropogenic global change in tropical savanna ecosystems.

Polycyclic aromatic hydrocarbons in samples of Ryugu formed in the interstellar medium.

Polycyclic aromatic hydrocarbons (PAHs) contain ≲20% of the carbon in the interstellar medium. They are potentially produced in circumstellar environments (at temperatures ≳1000 kelvin), by reactions within cold (~10 kelvin) interstellar clouds, or by processing of carbon-rich dust grains. We report isotopic properties of PAHs extracted from samples of the asteroid Ryugu and the meteorite Murchison. The doubly-13C substituted compositions (Δ2×13C values) of the PAHs naphthalene, fluoranthene, and pyrene are 9 to 51‰ higher than values expected for a stochastic distribution of isotopes. The Δ2×13C values are higher than expected if the PAHs formed in a circumstellar environment, but consistent with formation in the interstellar medium. By contrast, the PAHs phenanthrene and anthracene in Ryugu samples have Δ2×13C values consistent with formation by higher-temperature reactions.

Global response of fire activity to late Quaternary grazer extinctions.

Fire activity varies substantially at global scales because of the influence of climate, but at broad spatiotemporal scales, the possible effects of herbivory on fire activity are unknown. Here, we used late Quaternary large-bodied herbivore extinctions as a global exclusion experiment to examine the responses of grassy ecosystem paleofire activity (through charcoal proxies) to continental differences in extinction severity. Grassy ecosystem fire activity increased in response to herbivore extinction, with larger increases on continents that suffered the largest losses of grazers; browser declines had no such effect. These shifts suggest that herbivory can have Earth system­scale effects on fire and that herbivore impacts should be explicitly considered when predicting changes in past and future global fire activity.

Organic matter from the Chicxulub crater exacerbated the K-Pg impact winter.

An asteroid impact in the Yucatán Peninsula set off a sequence of events that led to the Cretaceous-Paleogene (K-Pg) mass extinction of 76% species, including the nonavian dinosaurs. The impact hit a carbonate platform and released sulfate aerosols and dust into Earth's upper atmosphere, which cooled and darkened the planet-a scenario known as an impact winter. Organic burn markers are observed in K-Pg boundary records globally, but their source is debated. If some were derived from sedimentary carbon, and not solely wildfires, it implies soot from the target rock also contributed to the impact winter. Characteristics of polycyclic aromatic hydrocarbons (PAHs) in the Chicxulub crater sediments and at two deep ocean sites indicate a fossil carbon source that experienced rapid heating, consistent with organic matter ejected during the formation of the crater. Furthermore, PAH size distributions proximal and distal to the crater indicate the ejected carbon was dispersed globally by atmospheric processes. Molecular and charcoal evidence indicates wildfires were also present but more delayed and protracted and likely played a less acute role in biotic extinctions than previously suggested. Based on stratigraphy near the crater, between 7.5 × 1014 and 2.5 × 1015 g of black carbon was released from the target and ejected into the atmosphere, where it circulated the globe within a few hours. This carbon, together with sulfate aerosols and dust, initiated an impact winter and global darkening that curtailed photosynthesis and is widely considered to have caused the K-Pg mass extinction.

Grassland fire ecology has roots in the late Miocene.

That fire facilitated the late Miocene C4 grassland expansion is widely suspected but poorly documented. Fire potentially tied global climate to this profound biosphere transition by serving as a regional-to-local driver of vegetation change. In modern environments, seasonal extremes in moisture amplify the occurrence of fire, disturbing forest ecosystems to create niche space for flammable grasses, which in turn provide fuel for frequent fires. On the Indian subcontinent, C4 expansion was accompanied by increased seasonal extremes in rainfall (evidenced by δ18Ocarbonate), which set the stage for fuel accumulation and fire-linked clearance during wet-to-dry seasonal transitions. Here, we test the role of fire directly by examining the abundance and distribution patterns of fire-derived polycyclic aromatic hydrocarbons (PAHs) and terrestrial vegetation signatures in n-alkane carbon isotopes from paleosol samples of the Siwalik Group (Pakistan). Two million years before the C4 grassland transition, fire-derived PAH concentrations increased as conifer vegetation declined, as indicated by a decrease in retene. This early increase in molecular fire signatures suggests a transition to more fire-prone vegetation such as a C3 grassland and/or dry deciduous woodland. Between 8.0 and 6.0 million years ago, fire, precipitation seasonality, and C4-grass dominance increased simultaneously (within resolution) as marked by sharp increases in fire-derived PAHs, δ18Ocarbonate, and 13C enrichment in n-alkanes diagnostic of C4 grasses. The strong association of evidence for fire occurrence, vegetation change, and landscape opening indicates that a dynamic fire-grassland feedback system was both a necessary precondition and a driver for grassland ecology during the first emergence of C4 grasslands.