RESUMEN
The conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI-MS spectrometry, and RP-HPLC. The GPx-like enzyme mimicking activity of the SeC-bioconjugates 1 and 2 has been investigated through the coupled reductase assay method for the catalytic reductions of hydrogen peroxide into water. A significant enhancement in GPx-like enzymatic activity was observed for both novel bioconjugates SeC-Biotin (1) and SeC-Lipoic acid (2) as compared to diphenyl diselenide (Ph2Se2), L-selenocystine (SeC), biotin, lipoic acid, and ebselen.
Asunto(s)
Compuestos de Organoselenio , Ácido Tióctico , Ácido Tióctico/química , Biotina , Glutatión Peroxidasa , Compuestos de Organoselenio/química , Antioxidantes/química , Glutatión/químicaRESUMEN
The detailed understanding and control of ionic transport pathways in the electrode/electrolyte interface is vital for realizing micro-scale energy storage devices and formulating adequate design principles. A planar device geometry with nanostructured thin solid polymer electrolyte (SPE) and potassium hydroxide (KOH) incorporated poly(N-vinyl imidazole) (PVI) is demonstrated for micro-supercapacitors (MSCs). The adsorption/desorption kinetics of ionic charges in the interfacial regime of ITO/PVI-KOH has been investigated for electrical double layer capacitance (EDLC) characteristics. A single-cell of ITO/PVI-KOH/ITO planar MSC shows the large variation in volumetric capacitance and capacitance retention characteristics when the thickness of PVI-KOH approaches the characteristic nanoscale. Moreover, ITO/PVI-KOH/ITO planar MSC consisting of five series-cells exhibits the maximum operating cell voltage of 5.0 V with maximum volumetric energy and power density of 0.056 mW h cm-3 and 6.89 mW cm-3, respectively. The electrochemical properties of planar MSC have been systematically studied so as to confirm how the anions and cations are separated at electrode/electrolyte interfaces by means of an electromotive force. Significantly, the hydrated PVI enables charge migration and separation of cations and anions at the electrode/electrolyte interfaces.