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1.
J Ethnopharmacol ; 333: 118402, 2024 Oct 28.
Artículo en Inglés | MEDLINE | ID: mdl-38821139

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: This review explores the link between Type 2 Diabetes Mellitus (T2DM) and diabetes-induced Alzheimer's disease (AD). It emphasizes the shared pathophysiological links and mechanisms between the two conditions, focusing on reduced insulin levels and receptors, impaired glucose metabolism, insulin resistance, mitochondrial dysfunction, and oxidative damage in AD-affected brains-paralleling aspects of T2DM. The review suggests AD as a "diabetes of the brain," supported by cognitive enhancement through antidiabetic interventions. It focuses on the traditionally used Indian herbs as a means to manage both conditions while addressing developmental challenges. AIM OF THE STUDY: This study explores the DM-AD connection, reviewing medicinal herbs with protective potential for both ailments, considering traditional uses and developmental challenges. MATERIALS AND METHODS: Studied research, reviews, and ethnobotanical and scientific data from electronic databases and traditional books. RESULTS: The study analyzes the pathophysiological links between DM and AD, emphasizing their interconnected factors. Eight Ayurvedic plants with dual protective effects against T2DM and AD are thoroughly reviewed with preclinical/clinical evidence. Historical context, phytoconstituents, and traditional applications are explored. Innovative formulations using these plants are examined. Challenges stemming from phytoconstituents' physicochemical properties are highlighted, prompting novel formulation development, including nanotechnology-based delivery systems. The study uncovers obstacles in formulating treatments for these diseases. CONCLUSION: The review showcases the dual potential of chosen medicinal herbs against both diseases, along with their traditional applications, endorsing their use. It addresses formulation obstacles, proposing innovative delivery technologies for herbal therapies, while acknowledging their constraints. The review suggests the need for heightened investment and research in this area.


Asunto(s)
Enfermedad de Alzheimer , Diabetes Mellitus Tipo 2 , Hipoglucemiantes , Plantas Medicinales , Enfermedad de Alzheimer/tratamiento farmacológico , Humanos , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Plantas Medicinales/química , Animales , Hipoglucemiantes/farmacología , Hipoglucemiantes/uso terapéutico , Fitoterapia , Extractos Vegetales/farmacología , Extractos Vegetales/química , Extractos Vegetales/uso terapéutico , Medicina Ayurvédica/métodos
2.
Phytother Res ; 38(6): 2764-2799, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38522945

RESUMEN

Rheumatoid arthritis is a chronic autoimmune inflammatory disease characterized by immune response overexpression, causing pain and swelling in the synovial joints. This condition is caused by auto-reactive antibodies that attack self-antigens due to their incapacity to distinguish between self and foreign molecules. Dysregulated activity within numerous signalling and immunological pathways supports the disease's development and progression, elevating its complexity. While current treatments provide some alleviation, their effectiveness is accompanied by a variety of adverse effects that are inherent in conventional medications. As a result, there is a deep-rooted necessity to investigate alternate therapeutic strategies capable of neutralizing these disadvantages. Medicinal herbs display a variety of potent bioactive phytochemicals that are effective in the complementary management of disease, thus generating an enormous potency for the researchers to delve deep into the development of novel phytomedicine against autoimmune diseases, although additional evidence and understanding are required in terms of their efficacy and pharmacodynamic mechanisms. This literature-based review highlights the dysregulation of immune tolerance in rheumatoid arthritis, analyses the pathophysiology, elucidates relevant signalling pathways involved, evaluates present and future therapy options and underscores the therapeutic attributes of a diverse array of medicinal herbs in addressing this severe disease.


Asunto(s)
Artritis Reumatoide , Fitoterapia , Plantas Medicinales , Artritis Reumatoide/tratamiento farmacológico , Humanos , Plantas Medicinales/química , Animales , Fitoquímicos/farmacología , Fitoquímicos/uso terapéutico
3.
Nat Prod Res ; 38(6): 1044-1048, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-37154600

RESUMEN

The rising geriatric population is expected to increase the demand for drugs treating neurodegenerative diseases. The present work is aimed to discover acetylcholinesterase (AChE) inhibitors from Cissampelos pareira Linn. aerial parts (Family: Menispermaceae). Bioassay-guided isolation, AChE inhibition study and estimation of the therapeutic marker in different parts of raw herbs were conducted. The structure of the compound (1) was elucidated as N-methylneolitsine by using NMR (1D and 2D) and ESI-MS/MS spectral data, which is a new natural analogue of neolitsine. It showed good AChE inhibition with an IC50 value of 12.32 µg/mL. It was densitometrically estimated to be 0.074 - 0.33% in aerial parts of C. pareira, collected from various locations. The alkaloid reported here could be potentially useful for the treatment of various neurodegenerative diseases and the aerial part of C. pareira could be used as a promising ingredient for various preparations treating neurodegenerative diseases.


Asunto(s)
Cissampelos , Menispermaceae , Enfermedades Neurodegenerativas , Anciano , Humanos , Cissampelos/química , Acetilcolinesterasa , Inhibidores de la Colinesterasa/farmacología , Espectrometría de Masas en Tándem , Extractos Vegetales/farmacología , Extractos Vegetales/química , Componentes Aéreos de las Plantas , Bioensayo
6.
Phytochem Anal ; 32(4): 629-639, 2021 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-33167083

RESUMEN

INTRODUCTION: Immunity boosting has emerged as a global strategy to fight the SARS-CoV-2 pandemic situation. In India, AYUSH systems of medicine have been promoted as an immune-protection strategy. Andrographis paniculata (Burm. F) Nees (AP) mentioned in Ayurveda has been widely used for treating sore throat, flu, and upper respiratory tract infections which may provide possible novel therapeutic approaches, exclusively targeting SARS-CoV-2 and its pathways. OBJECTIVES: The present work uses liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomics and combination synergy analysis based on network pharmacology to mine multimode evidence to understand the possible mechanism of action, diseases association, protein-protein interaction and major pathways involved therein. MATERIAL AND METHODS: Metabolite profiling was performed by Agilent QTOF LC-MS/MS system. Network pharmacology analysis was performed by using functional annotation analysis based on databases like Binding DB, STRING, DAVID and KEGG for further data mining. Further combination synergy was evaluated using "neighbourhood approach" and networks were constructed through Cytoscape 3.2.1. RESULTS: The molecules from kalmegh provides immune-protection and anti-viral response via involving different pathways, like toll-like receptor pathway, PI3/AKT pathway and MAP kinase pathways against COVID-19 infection. The KEGG analysis showed that in a vast majority of the most enriched pathways, AP were associated with viral infections and upper respiratory tract infections. CONCLUSIONS: The results suggest a synergy between andrographolide and other molecules identified as safe and efficacious anti-inflammatory agent having effects on upper respiratory tract infections and can significantly decrease the production of cytokines and pro-inflammatory factors in viral infections.


Asunto(s)
Andrographis , COVID-19 , Diterpenos , Cromatografía Liquida , Humanos , India , Medicina Ayurvédica , Extractos Vegetales/farmacología , SARS-CoV-2 , Espectrometría de Masas en Tándem
7.
J AOAC Int ; 103(3): 678-683, 2020 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-31540588

RESUMEN

BACKGROUND: Mucuna pruriens Bak. Syn. Mucuna prurita Hook. seed is the rich source of levodopa (L-dopa) and has been used in traditional medicines to treat diseases resembling Parkinson's disease. OBJECTIVE: In the present study, a new HPTLC method was developed and validated for estimation of L-dopa from M. pruriens (black- and white-colored seeds) collected from two different locations in India. METHOD: TLC aluminum plates precoated with silica gel were used as the stationary phase. The plates were developed to a distance of 60 mm at a temperature of 22 ± 4°C in a twin glass chamber saturated with ethyl acetate-methanol-formic acid-water (15+3+3+4.5, v/v/v/v), as the mobile phase. RESULTS: The Rf value of L-dopa was found to be 0.45. L-dopa was quantified at 282 nm, the wavelength of maximum absorbance by a densitometric scanner. The TLC plate was derivatized by ninhydrin reagent and photodocumented. L-dopa showed a good linearity in the concentration range 400-1000 ng/spot. The linear regression analysis of calibration plot showed good linear relationship between peak area and peak height (r2 = 0.997). CONCLUSIONS: The method was validated for precision, repeatability, and accuracy. Recovery was determined by spiking L-dopa with samples and was found to be 96.10%. HIGHLIGHTS: L-dopa was found to be present at concentration 3.02-4.72% in samples and its formulation.


Asunto(s)
Mucuna , Preparaciones Farmacéuticas , Cromatografía en Capa Delgada , India , Levodopa/análisis
8.
J AOAC Int ; 103(3): 692-698, 2020 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-31619314

RESUMEN

BACKGROUND: In Ayurvedic and Unani texts, many herbs and formulations are mentioned as complexion promoters having skin brightening and whitening effects that downregulate melanin synthesis. However, with the assistance of chromatographic techniques, further validation of scientific standardization is required. OBJECTIVE: To validate individual herbs and formulations in the ancient literature, some scientific aspects are to be focused on, including standardization of herbs or herbal products consisting of the active compounds. METHODS: Out of many herbs having complexion promoting properties, three herbs (Myristica fragrans, Hemidesmus indicus, and Inula racemosa) were selected for the study. In the present study, validated reverse phase (RP)-HPLC and high-performance TLC (HPTLC) methods were developed for standardization of the herbs. RESULTS: It was observed that the quercetin present in M. fragrans was 0.62% (w/w) with a retention time (Rt) of 6.23 min, the ferulic acid present in H. indicus was 1.39% (w/w) with an Rt of 8.083 min, and the chlorogenic acid present in I. racemosa was 1.03% (w/w) with an Rt of 3.19 min. The HPTLC method showed 1.23% (w/w) of quercetin present in M. fragrans with a retardation factor (Rf) of 0.48, ferulic acid in H. indicus was 1.52% (w/w) with an Rf of 0.44, and chlorogenic acid was 1.09% (w/w) in I. racemosa with an Rf of 0.52. CONCLUSIONS: This specific and precise validated method can be useful for the quality evaluation and quantitative determination of the constituents in complexion-promoting herbs from Ayurveda and Unani systems of medicine. HIGHLIGHTS: The exploration of complexion promoters from Ayurveda and Unani; quality evaluation of complexion promoters herbs from Ayurveda and Unani; HPTLC and RP-HPLC analysis of herbal extracts used and their validation.


Asunto(s)
Medicina Ayurvédica , Myristica , Cromatografía Líquida de Alta Presión , Extractos Vegetales , Quercetina/análisis
9.
J Ethnopharmacol ; 197: 165-172, 2017 Feb 02.
Artículo en Inglés | MEDLINE | ID: mdl-27457692

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Arishtas are Ayurvedic formulation made with decoction of herbs. Arjunarishta formulation is being used in Ayurveda for cardio-protective activity. Ashwagandharishta formulation possesses antioxidant, anti-atherosclerotic and anti-stress properties. Ridayarishta, a novel empirical formulation was prepared using combination of selected ingredients from these two formulations to support healthy heart functions and to reduce stress. AIM OF THE STUDY: Aim of the Study was to investigate herb-drug interaction (HDI) of Ridayarishta formulation through human hepatic cytochrome P450 (CYP450) enzyme inhibition assay. MATERIALS AND METHODS: Ridayarishta formulation was phyto-chemically standardized against arjunolic acid, arjunetin, berberine, piperine, resveratrol and withaferin-A using high performance thin layer chromatography (HPTLC) analysis. The formulation was standardized with respect to ethanol by gas chromatographic (GC) analysis. HDI was evaluated with Ridayarishta formulation and amlodipine besilate, atenolol, atorvastatin, metformin, glipizide glimepiride cocktail using high throughput CYP450 enzyme inhibition assay; against CYP1A2, 2C19, 2D6 and 3A4 isozymes. RESULTS: Contents of arjunolic acid, arjunetin, berberine, piperine, resveratrol and withaferin-A in Ridayarishta formulation were found to be 1.76±0.12, 1.51±0.09, 1.85±0.05, 3.2±0.12, 1.21±0.08, and 2.16±0.09ppm, respectively. Quantity of ethanol in Ridayarishta was found to be 7.95±0.023% (V/V). Ridayarishta showed significantly higher (P<0.001) IC50 value against CYP1A2 (IC50-13.80±1.96µg/mL), 2C19 (IC50-14.343±2.28µg/mL), 2D6 (IC50-0.897±0.28µg/mL) and 3A4 (IC50-32.057±2.51µg/mL) compared to positive controls such as furafylline, tranylcypromine, quinidine and ketoconazole respectively. Cocktail of herbal formulation and cardio protective, antihypertensive, anti-diabetic drugs showed significantly (P<0.001and P<0.01) less or negligible HDI. CONCLUSION: Ridayarishta formulation alone and cocktail with amlodipine besilate, atenolol, atorvastatin, metformin, glipizide, glimepiride had negligible or insignificant effect on CYP450 inhibition. It may be concluded that consumption of Ridayarishta along with selective cardio protective, antihypertensive and anti-diabetic conventional medicine is safe with negligible or without any significant CYP450 (CYP1A2, 2C19, 2D6 and 3A4) inhibition mediated HDI.


Asunto(s)
Inhibidores Enzimáticos del Citocromo P-450/farmacología , Sistema Enzimático del Citocromo P-450/metabolismo , Interacciones de Hierba-Droga/fisiología , Microsomas Hepáticos/metabolismo , Preparaciones Farmacéuticas/metabolismo , Extractos Vegetales/metabolismo , Extractos Vegetales/farmacología , Alcaloides/farmacología , Benzodioxoles/farmacología , Berberina/farmacología , Química Farmacéutica/métodos , Humanos , Medicina Ayurvédica , Piperidinas/farmacología , Alcamidas Poliinsaturadas/farmacología , Resveratrol , Saponinas/farmacología , Estilbenos/farmacología , Triterpenos/farmacología , Witanólidos/farmacología
10.
Phytomedicine ; 21(6): 847-56, 2014 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-24667331

RESUMEN

Rheumatoid arthritis (RA) is a chronic inflammatory disease which leads to destruction of joints. Current treatment modalities for RA either produce symptomatic relief (NSAIDs) or modify the disease process (DMARDs). Though effective, their use is also limited by their side effects. As a result, the interest in alternative, well tolerated anti-inflammatory remedies has re-emerged. Our aim was to evaluate the antioxidant and antiarthritic activity of Boswellia serrata gum resin extract (BSE) in collagen induced arthritis. Arthritis was induced in male Wistar rats by collagen induced arthritis (CIA) method. BSE was administered at doses of 100 and 200mg/kg body weight once daily for 21 days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, catalase, SOD and NO), inflammatory mediators (IL-1ß, IL-6, TNF-α, IL-10, IFN-γ and PGE2), and histological studies in joints. BSE was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, catalase, SOD and NO) studied. Oral administration of BSE resulted in significantly reduced levels of inflammatory mediators (IL-1ß, IL-6, TNF-α, IFN-γ and PGE2), and increased level of IL-10. The protective effects of BSE against RA were also evident from the decrease in arthritis scoring and bone histology. The abilities to inhibit proinflammatory cytokines and modulation of antioxidant status suggest that the protective effect of Boswellia serrata extract on arthritis in rats might be mediated via the modulation of immune system.


Asunto(s)
Antiinflamatorios/uso terapéutico , Antioxidantes/uso terapéutico , Artritis Experimental/tratamiento farmacológico , Boswellia , Mediadores de Inflamación/sangre , Estrés Oxidativo/efectos de los fármacos , Fitoterapia , Animales , Antiinflamatorios/farmacología , Antioxidantes/metabolismo , Antioxidantes/farmacología , Antirreumáticos/farmacología , Antirreumáticos/uso terapéutico , Artritis Experimental/sangre , Artritis Reumatoide/tratamiento farmacológico , Colágeno , Citocinas/sangre , Inflamación/sangre , Inflamación/inducido químicamente , Inflamación/tratamiento farmacológico , Masculino , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Ratas Wistar , Resinas de Plantas
11.
Cell Immunol ; 284(1-2): 51-9, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23921080

RESUMEN

OBJECTIVES: Piperine, a main component of Piper species, is a plant alkaloid with a long history of medical use in a variety of inflammatory disorders like rheumatoid arthritis. Due to side effects in current treatment modalities of rheumatoid arthritis, the interest in alternative, well tolerated anti-inflammatory remedies has re-emerged. The aim of this work was to evaluate the anti-inflammatory and antiarthritic effects of piperine. METHODS: Arthritis was induced in male Wistar rats by collagen induced arthritis (CIA) method. Piperine was administered at a dose of 100mgkg(-1) and indomethacin at 1mgkg(-1) body weight once daily for 21days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, Catalase, SOD and NO), inflammatory mediators (IL-1ß, TNF-α, IL-10 and PGE2) and histological studies in joints. RESULTS: Piperine was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, Catalase, SOD and NO) studied. Oral administration of piperine resulted in significantly reduced the levels of pro-inflammatory mediators (IL-1ß, TNF-α and PGE2) and increased level of IL-10. The protective effects of piperine against RA were also evident from the decrease in arthritis scoring and bone histology. CONCLUSIONS: In conclusion, the fact that piperine alter a number of factors known to be involved in RA pathogenesis indicates that piperine can be used similar to indomethacin as a safe and effective therapy for CIA and may be useful in the treatment of rheumatoid arthritis.


Asunto(s)
Alcaloides/farmacología , Artritis Experimental/tratamiento farmacológico , Artritis Reumatoide/tratamiento farmacológico , Benzodioxoles/farmacología , Piperidinas/farmacología , Alcamidas Poliinsaturadas/farmacología , Animales , Artritis Experimental/enzimología , Artritis Experimental/inmunología , Artritis Reumatoide/enzimología , Artritis Reumatoide/inmunología , Catalasa/análisis , Dinoprostona/análisis , Glutatión/análisis , Histocitoquímica , Inflamación/tratamiento farmacológico , Inflamación/enzimología , Inflamación/inmunología , Interleucina-10/análisis , Interleucina-1beta/análisis , Masculino , Óxido Nítrico/análisis , Elastasa Pancreática/análisis , Peroxidasa/análisis , Distribución Aleatoria , Ratas , Ratas Wistar , Superóxido Dismutasa/análisis , Factor de Necrosis Tumoral alfa/análisis
12.
Rheumatol Int ; 33(3): 657-63, 2013 03.
Artículo en Inglés | MEDLINE | ID: mdl-22527139

RESUMEN

Rheumatoid arthritis is a chronic inflammatory disease characterized by the destruction of articular cartilage and bone in a chronic phase. Pathology of rheumatoid arthritis suggests autoimmunity linked to inflammation. In our study, rheumatoid arthritis was induced in Wistar rats by intradermal injections of 100 µl of emulsion containing bovine type II collagen in complete Freund's adjuvant at the base of the tail. Disease developed about 13 ± 1 days after immunization and treatment with hesperidin (HES) at a dose of 160 mg kg(-1) body weight was given after onset of disease daily until 20th day. The effect of treatment in the rats was monitored by clinical scoring, biochemical parameters and histological evaluations in joints. A steady increase in the articular elastase, nitric oxide and lipid peroxidation was observed in joints of arthritic rats as compared to control, whereas a significant decrease in reduced glutathione, superoxide dismutase activity and catalase was observed in collagen-induced arthritis rats as compared to control group. The results from the present work indicate that the treatment with hesperidin was effective in bringing about significant changes on all the parameters studied in collagen-induced arthritis rats. These data confirm that erosive destruction of the joint cartilage in collagen-induced arthritis is due free radicals released by activated neutrophils and produced by other biochemical pathways. In the present study, an attempt has been made to amelioration of the disease process by a natural product. These results suggest that oral administration of HES could be effective for treating human RA patients.


Asunto(s)
Artritis Experimental/tratamiento farmacológico , Radicales Libres/metabolismo , Hesperidina/farmacología , Activación Neutrófila/efectos de los fármacos , Infiltración Neutrófila/efectos de los fármacos , Animales , Artritis Experimental/inmunología , Artritis Experimental/metabolismo , Catalasa/metabolismo , Hesperidina/uso terapéutico , Masculino , Óxido Nítrico/análisis , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismo
13.
Chem Biol Interact ; 197(1): 40-6, 2012 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-22450443

RESUMEN

Thymoquinone (TQ) is the major active compound derived from Nigella sativa. Our aim of this work was to evaluate the antioxidant and antiarthritic activity of TQ in Wistar rat by collagen induced arthritis (CIA). TQ was administered at a dose of 5mgkg(-1) body weight once daily for 21days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, catalase, SOD and NO), inflammatory mediators (IL-1ß, IL-6, TNF-α, IL-10, IFN-γ and PGE(2)) and histological studies in joints. TQ was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, catalase, SOD and NO) studied. Oral administration of TQ resulted in significantly reduced the levels of pro-inflammatory mediators (IL-1ß, IL-6, TNF-α, IFN-γ and PGE(2)) and increased level of IL-10. The protective effects of TQ against RA were also evident from the decrease in arthritis scoring and bone histology. In conclusion, the fact that TQ abolished a number of factors known to be involved in RA pathogenesis indicates that the administration of thymoquinone may have potential value in the treatment of inflammatory disease.


Asunto(s)
Antioxidantes/uso terapéutico , Artritis Experimental/tratamiento farmacológico , Benzoquinonas/uso terapéutico , Citocinas/inmunología , Nigella sativa/química , Estrés Oxidativo/efectos de los fármacos , Animales , Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Artritis Experimental/inmunología , Artritis Experimental/metabolismo , Artritis Experimental/patología , Benzoquinonas/aislamiento & purificación , Benzoquinonas/farmacología , Catalasa/metabolismo , Glutatión/metabolismo , Articulación de la Rodilla/efectos de los fármacos , Articulación de la Rodilla/inmunología , Articulación de la Rodilla/metabolismo , Articulación de la Rodilla/patología , Masculino , Óxido Nítrico/metabolismo , Elastasa Pancreática/metabolismo , Peroxidasa/metabolismo , Ratas , Ratas Wistar , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo
14.
Bioorg Med Chem ; 18(11): 3940-5, 2010 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-20452777

RESUMEN

Bioassay guided fractionation of the hydro-alcoholic extract of the fruits of Capparis moonii, led to the isolation of two new chebulinic acid derivatives. The compounds 1 and 2 displayed significant glucose uptake effect of 223% and 219% over the control at the 10ng/ml and 100ng/ml concentration, respectively. The increased glucose uptake effects of the compounds were associated with significant IR and IRS-1 phosphorylation, GLUT4 and PI3-kinase mRNA expression in the L6 cells.


Asunto(s)
Capparis/química , Glucosa/metabolismo , Taninos Hidrolizables/aislamiento & purificación , Taninos Hidrolizables/farmacología , Animales , Línea Celular , Transportador de Glucosa de Tipo 4/genética , Proteínas Sustrato del Receptor de Insulina/metabolismo , Imitación Molecular , Fosfatidilinositol 3-Quinasas/genética , Fosforilación/efectos de los fármacos , Fitoterapia , ARN Mensajero/análisis , ARN Mensajero/efectos de los fármacos , Ratas , Receptor de Insulina/metabolismo
15.
Int J Ayurveda Res ; 1(4): 243-6, 2010 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-21455453

RESUMEN

A new, simple, sensitive, selective, and precise high-performance liquid chromatography (HPLC) method for analysis of berberine in crude plant material, herbal extract, and ayurvedic dosage forms was developed and validated. The stationary phase was inert sil C(18) column The mobile phase consisting of acetonitrile (HPLC Grade) and potassium dihydrogen phosphate buffer (pH 2.5) in a gradient flow was used.The column was equilibrated with the mobile phase (flow rate 1.0 ml/min); elution was monitored at 346 nm. The linear regression analysis data for the calibration plots showed good linear relationship, with r(2)= 0.9942 in the concentration range of 16380-30420 µg/ml with respect to the peak area. The method was validated for specificity, precision, recovery, and linearity according to the International Conference on Harmonization guidelines. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of berberine.

16.
Planta Med ; 74(10): 1308-15, 2008 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-18553273

RESUMEN

The quality control of medicinal herbs post harvesting or after collection becomes very critical because of susceptibility to fungal invasion during storage depending on the temperature and humidity of the storage area. The information on moisture equilibrium is important on the process and storage of foods which can be extended to medicinal herbs. In the present study, the growth of Aspergillus flavus was observed on selected ten medicinal herbs with water activity aw above 0.81 when stored at 25 +/- 2 degrees C, 30 +/- 2 degrees C and 40 +/- 2 degrees C except for Picrorhiza kurrooa and Alpinia galanga which were found to have anti-fungal properties. Aspergillus flavus did not grow in any samples of medicinal herbs with water activity aw below 0.81 at temperatures of 25 +/- 2 degrees C, 30 +/- 2 degrees C and 40 +/- 2 degrees C. Also Aspergillus flavus did not grow in any samples of medicinal herbs with water activity aw above 0.81 when stored below 10 +/- 2 degrees C. Therefore it can be concluded that the contamination of medicinal herbs with aflatoxins can be minimized by controlling water activity and storage temperature. Sorption isotherms (desorption) can be interpreted to determine the optimum drying which can lower the water activity to the level required for preventing growth of Aspergillus flavus and also for ensuring quality of medicinal herbs which may get destroyed upon over drying. Furthermore, it also saves incremental cost in prolonged drying over the optimum drying.


Asunto(s)
Aspergillus flavus/crecimiento & desarrollo , Plantas Medicinales/microbiología , Temperatura , Agua/análisis , Desecación , Plantas Medicinales/química , Control de Calidad
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