RESUMEN
Hepatitis E is a viral infectious disease in pigs, wild boars, cows, deer, rabbits, camels, and humans as hosts caused by Paslahepevirus. Recently, it has been detected in a wide variety of animals including domestic small ruminants. Mongolia is a land of nomadic people living with livestock such as sheep, goats, and cattle. Due to how Mongolian lifestyles have changed, pork has become popular and swine diseases have emerged. Among them, Hepatitis E disease has become a zoonotic infectious disease that needs to be addressed. The HEV problem in pigs is that infected pigs excrete the virus without showing clinical symptoms and it spreads into the environment. We attempted to detect HEV RNA in sheep which had been raised in Mongolia for a long time, and those animals living together with pigs in the same region currently. We also conducted a longitudinal analysis of HEV infection in pigs in the same area and found that they were infected with HEV of the same genotype and cluster. In this study, we examined 400 feces and 120 livers (pigs and sheep) by RT-PCR in Töv Province, Mongolia. HEV detection in fecal samples was 2% (4/200) in sheep and 15% (30/200) in pigs. The results of ORF2 sequence analysis of the HEV RT-PCR-positive pigs and sheep confirmed genotype 4 in both animals. The results suggest that HEV infection is widespread in both pigs and sheep and that urgent measures to prevent infection are needed. This case study points to the changing nature of infectious diseases associated with livestock farming. It will be necessary to reconsider livestock husbandry and public health issues based on these cases.
RESUMEN
Autoimmune, allergic, and respiratory inflammatory diseases are some of the most important health issues worldwide. Disorders of the gut microbiota have been associated with the induction of allergic and inflammatory diseases, and probiotics are being tested for disease prevention. We examined functional Lactiplantibacillus plantarum RGU (Lp-1) to mice with ovalbumin (OVA)-induced asthma model to elucidate the inhibitory effect on pathological progression in asthma model. Prior to the experiments, the intestinal lactic acid bacteria were reduced by administering multiple antibiotics (MAB) to evaluate the administration effect of lactic acid bacteria. Mice were administered with Lp-1 or comparative control lactic acid bacteria in each group. After that, OVA-induced asthma was induced, and cytokine gene expression and histological findings were compared. Exacerbation of lung lesions was confirmed in the MAB group. The Lp-1 group mice had alleviated lung lesions with a decrease in IL-1ß, IL-13, IL-17 and an increase in IL-10 of the splenocytes and bronchial lymph nodes compared with the MAB group, but not in the other groups. In OVA-induced asthma, administration of specific Lactiplantibacillus was confirmed to induce anti-inflammatory cytokines.
Asunto(s)
Asma , Animales , Antiinflamatorios/uso terapéutico , Asma/inducido químicamente , Líquido del Lavado Bronquioalveolar , Citocinas/metabolismo , Modelos Animales de Enfermedad , Pulmón/patología , Ratones , Ratones Endogámicos BALB C , OvalbúminaRESUMEN
Monoclonal antibodies (mAbs) that recognize cluster of differentiation (CD) molecules on lymphocytes are useful tools for the study of different lymphocyte subsets in flow cytometry (FCM) analysis. CD4 is a glycoprotein found on the surfaces of helper T cells, monocytes, macrophages, and dendritic cells. In this study, we describe Japanese Black (JB) calves in a farm whose peripheral blood mononuclear cells (PBMCs) did not react with a CD4-specific mAb. To identify calves with PBMCs with low mAb reactivity, PBMCs from 21 JB calves (1-12 months of age) bred at the same farm were examined using two different bovine CD4 mAbs (clones #CC8 and #CACT138A). FCM analysis indicated that the calves fell into two groups based on reactivity against the two mAbs, i.e., double-positive (DP) calves, whose PBMCs were recognized by both mAbs clones, and single-positive (SP) calves, whose PBMCs were only recognized by #CACT138A. PBMCs from seven calves were not recognized by #CC8, although they had normal reactivity with another mAb, #CACT138A. Sequencing analysis of the CD4 gene in these calves revealed four nucleotide substitutions (G918 T, A930C, G970A, and G1074A) in the coding region in the SP group when compared to the DP group. Three of the four mutations were associated with amino acid substitution (Q306H, K310 N, and A324 T). The substitution at A324 T was located in the D4 domain of CD4 gene. Homology modeling based on the amino acid sequences revealed that the surface structure of this part of the molecule was significantly different between the SP and the DP groups. Therefore, the epitope recognized by the #CC8 CD4 mAb was altered in calves with this genetic mutation, and this led to the low reactivity of the PBMCs from calves in the SP group aginst the #CC8 mAb. In conclusion, this is the first study to identify CD4 variants in JB cattle. We confirmed that the variants did not affect lymphocyte functions, such as mitogen stimulation or lipopolysaccharide-induced cytokine gene expression.
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Anticuerpos Monoclonales/inmunología , Antígenos CD4/genética , Antígenos CD4/inmunología , Bovinos/inmunología , Leucocitos Mononucleares/inmunología , Sustitución de Aminoácidos/genética , Animales , Recuento de Células Sanguíneas/veterinaria , Antígenos CD4/química , Bovinos/genética , Citometría de Flujo/veterinaria , Modelos Moleculares , Estructura Molecular , MutaciónRESUMEN
The composition of the intestinal microbiota is related to the health and immune function of the host. Administration of antibiotics affects the composition of the intestinal microbiota. However, the effects of immune function on the composition of the intestinal microbiota are still unclear. In this study, we investigated the lymphocyte composition and determined the relationships between lymphocyte function and the intestinal microbiota following antibiotic treatment in mice. To change the composition of the intestinal microbiota, mice were treated with or without antibiotics. Analysis of intestinal microbiota was performed by metagenomic analysis targeting 16S rRNA. Lymphocyte subsets of splenocytes were measured by flow cytometry. For functional analysis of T cells, splenocytes were stimulated with concanavalin (Con A), and cytokine gene expression was measured by real-time polymerase chain reaction. Firmicutes were predominant in the control group, whereas Bacteroidetes predominated in the antibiotic-treated group, as determined by metagenomic analysis. The diversity of the microbiota decreased in the antibiotic-treated group. Analysis of lymphocyte subsets showed that CD3+ cells decreased, whereas CD19+ cells increased in the antibiotic-treated group. All cytokine genes in splenocytes treated with Con A were downregulated in the antibiotic-treated group; in particular, genes encoding interferon-γ, interleukin (IL)-6, and IL-13 significantly decreased. Taken together, these results revealed that changes in the composition of the intestinal microbiota by antibiotic treatment influenced the population of lymphocytes in splenocytes and affected the immune response.
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Microbioma Gastrointestinal/inmunología , Inmunidad/fisiología , Animales , Antibacterianos/farmacología , Concanavalina A/metabolismo , Citocinas/metabolismo , Citometría de Flujo , Microbioma Gastrointestinal/efectos de los fármacos , Inmunidad/inmunología , Ratones/inmunología , Ratones/microbiología , Ratones Endogámicos BALB C/inmunología , Ratones Endogámicos BALB C/microbiología , ARN Ribosómico 16S , Reacción en Cadena en Tiempo Real de la Polimerasa , Bazo/citología , Subgrupos de Linfocitos T/inmunología , Subgrupos de Linfocitos T/fisiologíaRESUMEN
The objectives of our research on equine piroplasmosis were to clarify the prevalence of the piroplasms Babesia caballi and Theileria equi among reintroduced Przewalski's horses (takhi, Equus ferus przewalskii) in comparison with horses (Equus caballus) of nomads from around Hustai National Park in Mongolia and to determine the mortality among takhi during 2012 to 2015. The prevalence of T. equi was high in 2014 at 80% among nomadic horses and 84% among takhi. We found no evidence of B. caballi infection among takhi. A homology comparison of the 18S rRNA base sequence of T. equi showed the same sequence in both domestic horses and takhi. According to the national park records, piroplasmosis is a cause of high mortality in young takhi (19%). The results suggested that takhi were infected with T. equi in this park, and the infection is an important issue when reintroducing the takhi into the wild and increasing the numbers of the animals in nature. Continuous monitoring and vector control were noted as important issues in future conservation programs.
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Babesiosis/prevención & control , ADN Protozoario/química , Enfermedades de los Caballos/prevención & control , Theileria/genética , Theileriosis/prevención & control , Animales , Animales Domésticos , Animales Salvajes , Babesiosis/epidemiología , ADN Protozoario/sangre , ADN Protozoario/aislamiento & purificación , Especies en Peligro de Extinción , Enfermedades de los Caballos/epidemiología , Enfermedades de los Caballos/parasitología , Caballos , Filogenia , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Theileria/clasificación , Theileriosis/epidemiología , Infestaciones por Garrapatas/complicaciones , Infestaciones por Garrapatas/epidemiología , Infestaciones por Garrapatas/veterinariaRESUMEN
The intestinal microbiota of newborn calves changes during the early postnatal period and influences their health and immune function. We studied the compositional changes in the intestinal microbiome of newborn calves during the first week after birth by metagenomic analysis. In feces from newborn calves, we identified 4 bacterial phyla, namely, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The relative abundance of Lactobacillaceae significantly increased from day 1 to day 7. We evaluated Lactobacillus spp. colony numbers using selective agar plates and confirmed that the abundance of Lactobacillus spp. significantly increased during the first 7 days after birth. In conclusion, Lactobacillus spp. colonized the intestinal tract of calves during the first 7 days after birth.
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Bovinos/microbiología , Microbioma Gastrointestinal , Lactobacillus/crecimiento & desarrollo , Animales , Animales Recién Nacidos , Bacterias/aislamiento & purificación , Heces/microbiología , Intestinos/microbiología , Lactobacillus/aislamiento & purificación , Tipificación MolecularRESUMEN
BACKGROUND: Hepatitis E virus (HEV) transmitted via the oral route through the consumption of contaminated water or uncooked or undercooked contaminated meat has been implicated in major outbreaks. Rats may play a critical role in HEV outbreaks, considering their negative effects on environmental hygiene and food sanitation. Although the serological evidence of HEV infection in wild rodents has been reported worldwide, the infectivity and propagation of HEV in wild rats remain unknown. To investigate if rats are a possible carrier of HEV, we studied wild Norway rats (Rattus norvegicus) that were caught near a pig farm, where HEV was prevalent among the pigs. METHODS: We examined 56 Norway rats for HEV. RNA from internal organs was examined for RT-PCR and positive samples were sequenced. Positive tissue samples were incubated with A549 cell line to isolate HEV. Anti-HEV antibodies were detected by ELISA. RESULTS: Sixteen rats were seropositive, and the HEV RNA was detected in 10 of the 56 rats. Sequencing of the partial ORF1 gene from 7 samples resulted in partially sequenced HEV, belonging to genotype 3, which was genetically identical to the HEV prevalent in the swine from the source farm. The infectious HEVs were isolated from the Norway rats by using the human A549 cell line. CONCLUSIONS: There was a relatively high prevalence (17.9%) of the HEV genome in wild Norway rats. The virus was mainly detected in the liver and spleen. The results indicate that these animals might be possible carrier of swine HEV in endemic regions. The HEV contamination risk due to rats needs to be examined in human habitats.
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Lactobacillus gasseri OLL 2716 promotes the elimination of Helicobacter pylori and is utilized in yogurts that are specifically labeled as health foods. On the other hand, milk whey fermented by Propionibacterium freudenreichii ET-3, which increases the numbers of Bifidobacterium, is effective for intestinal disorders. We previously demonstrated that oral administration of L. gasseri and P. freudenreichii fermentation metabolites (LP-FM) improved calf intestinal microflora and reduced the incidence of diarrhea. However, the detailed immunological mechanisms responsible for these effects remain to be fully understood. In this study, we investigated whether LP-FM stimulates the innate immune response and promotes the elimination of Listeria monocytogenes in mice. The C57BL/6 female mice that were treated with LP-FM or L. gasseri fermentation metabolites alone for 4 weeks had more peripheral white blood cells than the untreated control mice. In particular, LP-FM-treated mice had higher CD4- and CD8-positive T-cell counts. The levels of reactive oxygen and nitrogen species produced by peritoneal macrophages were also higher in LP-FM-treated mice. Furthermore, LP-FM-treated mice that were infected with L. monocytogenes exhibited significant enhancement of the elimination of Listeria from the spleen and the liver in comparison with untreated control mice infected with Listeria. The activation of innate immunity by LP-FM was increased by the combination of fermentation metabolites from P. freudenreichii. These results suggest that LP-FM, which contains metabolites from L. gasseri and P. freudenreichii, stimulates the function of the innate immune system, thereby significantly promoting the elimination of L. monocytogenes in mice.
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Factores Inmunológicos/metabolismo , Factores Inmunológicos/uso terapéutico , Inmunomodulación/efectos de los fármacos , Lactobacillus/metabolismo , Listeriosis/inmunología , Propionibacterium/metabolismo , Animales , Medios de Cultivo Condicionados , Citocinas/genética , Citocinas/metabolismo , Femenino , Fermentación , Regulación de la Expresión Génica/efectos de los fármacos , Inmunidad Innata/efectos de los fármacos , Factores Inmunológicos/farmacología , Recuento de Leucocitos , Leucocitos/efectos de los fármacos , Listeria monocytogenes , Listeriosis/metabolismo , Listeriosis/prevención & control , Hígado/efectos de los fármacos , Hígado/microbiología , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/metabolismo , Ratones , Ratones Endogámicos C57BL , Especies de Nitrógeno Reactivo/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Bazo/citología , Bazo/efectos de los fármacos , Bazo/metabolismo , Bazo/microbiologíaRESUMEN
Skeletal muscle satellite cells are quiescent stem cells that localized between the plasmalemma and the basement membrane of muscle fiber. When muscle is injured, satellite cells are activated, migrate to the injured site and contribute to the regeneration of muscle. However, little is known about the mechanism by which satellite cells migrate underneath the basement membrane. To clarify this, we investigated the effect of MMP inhibition on the migration of C2C12 muscle cells in vitro using a time-lapse imaging system. The migration speed of cells cultured with an MMP-inhibitor reagent was significantly lower (P<0.01) than the control cultured without an inhibitor reagent. The persistency index was significantly higher (P<0.01) in cells cultured with an inhibitor than in those without an inhibitor. Furthermore, MMP-3 knockdown cells migrated faster than control cells. These results strongly suggest that MMPs synthesized in skeletal muscle cells play an important role in the migration of these cells.
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Metaloproteinasas de la Matriz/metabolismo , Mioblastos/fisiología , ARN Interferente Pequeño/metabolismo , Células Satélite del Músculo Esquelético/fisiología , Animales , Línea Celular , Movimiento Celular , Células Cultivadas , Inhibidores de la Metaloproteinasa de la Matriz , Ratones , Mioblastos/citología , Ratas , Ratas Sprague-Dawley , TransfecciónRESUMEN
Beta-Catenin not only acts as a regulator of E-cadherin-mediated cell-cell adhesion but also plays an important role in Wnt signaling. To assess the prevalence of Wnt signaling, we examined beta-catenin mutation and its immunohistochemical protein expression in oral cancers. The results were linked with expression of cyclin D1, one of the target genes of Wnt signaling, expression of epidermal growth factor receptor (EGFR) relevant to beta-catenin tyrosine phosphorylation, Ki-67 labeling index, clinicopathological features, and survival. In the analysis based on membranous expression of beta-catenin, 75 (68.2%) of 110 cases showed a reduced membranous pattern, and the remaining 35 (31.8%) had a preserved membranous pattern similar to that in oral epithelium. In the analysis of another category of beta-catenin expression, a cytoplasmic/nuclear pattern was observed in 21 (19.1%) of the 110 tumors. Most (19/21, 90.5%) of these tumors had a concomitant reduction of membranous expression of beta-catenin. The reduced membranous or cytoplasmic/nuclear pattern of beta-catenin was significantly associated with an invasive growth pattern, EGFR expression, an increased Ki-67 labeling index, and shorter survival but not with cyclin D1 expression. Mutational analyses of beta-catenin were performed for 39 cases, including the 21 tumors with a cytoplasmic/nuclear pattern, but no mutations in the beta-catenin gene exon 3 were detected in these samples. Our data indicate that altered expression of beta-catenin may play an important role in tumor progression through increased proliferation and invasiveness under EGFR activation. However, mutations of beta-catenin do not appear to be responsible for tumor development and abnormal expression of beta-catenin in oral cancers.
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Ciclina D1/genética , Proteínas del Citoesqueleto/genética , Receptores ErbB/genética , Expresión Génica , Antígeno Ki-67/análisis , Neoplasias de la Boca/genética , Transactivadores/genética , Adulto , Anciano , Anciano de 80 o más Años , Carcinoma de Células Escamosas/química , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patología , Ciclina D1/análisis , Proteínas del Citoesqueleto/análisis , Análisis Mutacional de ADN , Epitelio/química , Receptores ErbB/análisis , Femenino , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Neoplasias de la Boca/química , Neoplasias de la Boca/patología , Mutación , Reacción en Cadena de la Polimerasa , Tasa de Supervivencia , Transactivadores/análisis , beta CateninaRESUMEN
We have recently shown that cyclic AMP (cAMP) increases claudin-5 immunoreactivity along cell boundaries and could promote phosphorylation of claudin-5 on threonine residues in porcine blood-brain barrier (BBB) endothelial cells via a protein kinase A (PKA)-dependent pathway (Exp. Cell Res. 290 [2003] 275). Along this line, we identified a putative phosphorylation site for PKA at Thr(207) in the intracytoplasmic carboxyl terminal domain of claudin-5. To clarify the biological significance of this site in regulation of endothelial barrier functions, we established rat lung endothelial (RLE) cells expressing doxycycline (Dox)-inducible wild-type claudin-5 and a mutant with a substitution of Ala for Thr(207) (CL5T207A). We show that induction of wild-type claudin-5 is sufficient to reconstitute the paracellular barrier against inulin (5 kDa), but not mannitol (182 Da), in leaky RLE cells. By contrast, the barrier against both molecules was induced in the mutant cells. We also demonstrate that, upon cAMP treatment, Thr(207) of claudin-5 is involved in enhancement of claudin-5 immunoreactive signals along cell borders, rapid reduction in transendothelial electrical resistance (TER), and loosening of the claudin-5-based endothelial barrier against mannitol, but not inulin. cAMP decreased the claudin-5-based endothelial barrier, strongly suggesting that other tight-junction molecule(s) are required to elevate endothelial barrier functions in response to cAMP.