1.
J Microbiol Methods
; 55(3): 801-5, 2003 Dec.
Artículo
en Inglés
| MEDLINE
| ID: mdl-14607424
RESUMEN
3-hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.
Asunto(s)
Acetileno/análogos & derivados , Burkholderia cepacia/metabolismo , Catecoles/metabolismo , Colorantes Fluorescentes/metabolismo , Pseudomonas/metabolismo , Ralstonia/metabolismo , Tolueno/metabolismo , Acetileno/metabolismo , Burkholderia cepacia/enzimología , Microscopía Fluorescente , Pseudomonas/enzimología , Ralstonia/enzimología
2.
Science
; 299(5609): 1027-9, 2003 Feb 14.
Artículo
en Inglés
| MEDLINE
| ID: mdl-12586932