Evaluation of fuel properties for possible biodiesel output based on the fatty acid composition of oleaginous plants and microalgae.

In the past decade, there has been a significant rise in sustainable biomass based biofuel production to address energy needs while mitigating environmental impacts. Traditionally, bioethanol was used for biofuel production, but concerns over food security and environmental preservation have led to growing interest in alternative sources such as neutral lipids from vegetable oil and microalgae for biodiesel production. This research paper evaluates the potential of various oleaginous plants and microalgae as feedstocks for biodiesel production, with a focus on their fatty acid composition and its impact on biodiesel properties. The study examines the fatty acid profiles of 43 different plant and microalgae species and employs various equations to estimate key physical properties of biodiesel. Additionally, the communication compares these properties to International Biodiesel Standards (EN 14214 and ASTM D6751-08) to assess the suitability of the derived biodiesel for commercial use. It is impossible to describe a single composition that is optimal in terms of all essential fuel properties due to the opposing effects of some structural features of the Fatty Acid Methyl Esters (FAME). However, biodiesel should contain relatively low concentrations of both long chain saturated and polyunsaturated FAME to ensure adequate efficiency in terms of low temperature operability and oxidative stability. The results reveal significant variations in properties amongst different feedstocks, highlighting the importance of feedstock selection in biodiesel production. The study also establishes correlations between various fuel properties, providing valuable insights in to optimizing biodiesel production processes, which will be of great use to researchers, engineers, and stakeholders involved in biodiesel production.

Characterization and application of novel fly ash blended ceramic membrane in MFC for low-cost and sustainable wastewater treatment and power generation.

Field-scale application of the microbial fuel cell (MFC) technology faces a major constraint due to the widely used high-cost proton exchange membrane Nafion, prompting lately, the development of ceramic membranes using different clay minerals. In the present study, the characteristics and applicability of a novel ceramic membrane fabricated using potter's clay (C) blended with varying proportions (0, 5, 10, and 20 wt%) of fly ash (FA), designated as CFA0, CFA5, CFA10, and CFA20, were assessed for cost-effective and sustainable use in MFC. On assessing the properties of the membrane, CFA10 was found to exhibit superior quality with fine pore size distribution (average 0.49 µm) favoring higher water uptake and less oxygen diffusion. The CFA10 membrane showed a maximum proton mass transfer coefficient (4.32 ± 0.04 × 10-5 cm/s) that was about three times that of the control CFA0. The oxygen mass transfer coefficient of CFA10 was 5.13 ± 0.12 × 10-5 cm/s, which was about 40% less than in the control. X-ray diffraction (XRD) analysis of CFA membrane revealed the richness of quartz, which facilitates proton conductance and water retention. The CFA10 membrane fitted MFC demonstrated a peak power output of 4.57 W/m3 (twice that in CFA0) with an average of 80.02 ± 0.86% COD removal and 68.03 ± 0.13% coulombic efficiency in a long-term study indicating its improved applicability and durability. Electrochemical kinetics involving cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) also affirmed the efficacy of CFA10 membrane in MFC showing peak current output of 13.95 mA and low ohmic resistance (74.2 Ω). The novel (CFA10) ceramic membrane amalgamated with the coal fly ash, a waste of concern, shows promise for high MFC performance at a much reduced (98% less) cost that can be used for sustainable scale-up of the technology.

Sustained energy production from wastewater in microbial fuel cell: effect of inoculum sources, electrode spacing and working volume.

The present study was aimed at producing enhanced and sustained bioelectricity from distillery wastewater in a double chamber microbial fuel cell (MFC) by changing inter-electrode distance, inoculum and reactor volume. Using double chamber MFC with 1 L working volume, when the distance between the electrodes was kept shorter (1 cm), it generated power density of 1.74 W/m3, which was 42.5% higher than that of MFC with electrode spacing of 10 cm (1 W/m3). Using inoculum from different sources viz. garden soil (MFC-GS), wetland sediment (MFC-WS) and sludge from wastewater treatment plant (MFC-S), the highest open circuit voltage (OCV) of 0.84 V and power density of 2.74 W/m3 were produced by MFC-WS, which also showed sustained electricity production (1.68 W/m3) from the wastewater during a 10-day experiment. Relatively lower power density was generated from MFC-S (1.42 W/m3), while that from MFC-GS was the lowest (0.94 W/m3). Bioelectricity generation and overall performance were then assessed using a smaller reactor size. Smaller working volume of MFC (250 ml) favoured greater production of power density (3.2 W/m3) than that with 1 L working volume (2.96 W/m3) with electrode distance of 1 cm. The present study was novel in selecting a suitable mixed-microbial inoculum out of the diverse sources screened and reducing resistance by sharply narrowing down inter-electrode distance and reactor volume, which led to significantly enhanced and sustained electricity generation from double chamber MFC.

Suitability assessment of dumpsite soil biocover to reduce methane emission from landfills under interactive influence of nutrients.

Biocovers are known for their role as key facilitator to reduce landfill methane (CH4) emission on improving microbial methane bio-oxidation. Methanotrophs existing in the aerobic zone of dumped wastes are the only known biological sinks for CH4 being emitted from the lower anaerobic section of landfill sites and even from the atmosphere. However, their efficacy remains under the influence of landfill environment and biocover characteristics. Therefore, the present study was executed to explore the suitability and efficacy of dumpsite soil as biocover to achieve enhanced methane bio-oxidation under the interactive influence of nutrients, carbon source, and environmental factors using statistical-mathematical models. The Placket-Burman design (PBD) was employed to identify the significant factors out of 07 tested factors having considerable impact on CH4 bio-oxidation. The normal plot and Student's t test of PBD indicated that ammonical nitrogen (NH4+-N), nitrate nitrogen (NO3--N), methane (CH4), and copper (Cu) concentration were found significant. A three-level Box-Behnken design (BBD) was further applied to optimize the significant factors identified from PBD. The BBD results revealed that interactive interaction of CH4 with NH4+-N and NO3--N affected the CH4 bio-oxidation significantly. The sequential statistical approach predicted that maximum CH4 bio-oxidation of 27.32 µg CH4 h-1 could be achieved with CH4 (35%), NO3--N (250 µg g-1), NH4+-N (25 µg g-1), and Cu (50 mg g-1) concentration. Conclusively, waste dumpsite soil could be a good alternative over conventional soil cover to improve CH4 bio-oxidation and lessen the emission of greenhouse gas from waste sector.

Bioassays for toxicological risk assessment of landfill leachate: A review.

Landfilling is the most common solid waste management practice. However, there exist a potential environmental risk to the surface and ground waters due to the possible leaching of contaminants from the landfill leachates. Current municipal solid waste landfill regulatory approaches consider physicochemical characterization of the leachate and do not assess their potential toxicity. However, assessment of toxic effects of the leachates using rapid, sensitive and cost-effective biological assays is more useful in assessing the risks as they measure the overall toxicity of the chemicals in the leachate. Nevertheless, more research is needed to develop an appropriate matrix of bioassays based on their sensitivity to various toxicants in order to evaluate leachate toxicity. There is a need for a multispecies approach using organisms representing different trophic levels so as to understand the potential impacts of leachate on different trophic organisms. The article reviews different bioassays available for assessing the hazard posed by landfill leachates. From the review it appears that there is a need for a multispecies approach to evaluate leachate toxicity.

Statistical assessment of dumpsite soil suitability to enhance methane bio-oxidation under interactive influence of substrates and temperature.

Biocovers are considered as the most effective and efficient way to treat methane (CH4) emission from dumpsites and landfills. Active methanotrophs in the biocovers play a crucial role in reduction of emissions through microbiological methane oxidation. Several factors affecting methane bio-oxidation (MOX) have been well documented, however, their interactive effect on the oxidation process needs to be explored. Therefore, the present study was undertaken to investigate the suitability of a dumpsite soil to be employed as biocover, under the influence of substrate concentrations (CH4 and O2) and temperature at variable incubation periods. Statistical design matrix of Response Surface Methodology (RSM) revealed that MOX rate up to 69.58µgCH4g-1dwh-1 could be achieved under optimum conditions. MOX was found to be more dependent on CH4 concentration at higher level (30-40%, v/v), in comparison to O2 concentration. However, unlike other studies MOX was found in direct proportionality relationship with temperature within a range of 25-35°C. The results obtained with the dumpsite soil biocover open up a new possibility to provide improved, sustained and environmental friendly systems to control even high CH4 emissions from the waste sector.

FTIR spectroscopy and scanning electron microscopic analysis of pretreated biosorbent to observe the effect on Cr (VI) remediation.

Various chemical and physical treatments have been applied to indigenously isolated cyanobacterial strain, Lyngbya putealis HH-15, to observe the effect on chromium removal capacity. Pretreatment with hydrochloric acid (99.1%) and nitric acid (98.5%) resulted in enhanced chromium removal as compared to untreated control biosorbent (98.1%). Pretreatment with acetic acid (97.9%), methanol (97.0%), calcium chloride (96.0%), hot water (95.2%), and sodium hydroxide (93.9%) did not improve the chromium removal capacity of biosorbent. Fourier transform infrared spectrometry (FTIR) and scanning electron microscopy (SEM) analysis identified changes in biomass functionality and availability after physical and chemical modification-the results of which were in agreement with metal removal studies. In conclusion, this acid-treated biosorbent represents a suitable candidate to replace conventional removal technologies for metal-bearing wastewaters.

Evaluation and statistical optimization of methane oxidation using rice husk amended dumpsite soil as biocover.

A laboratory scale study was conducted to investigate the effect of rice husk amended biocover to mitigate the CH4 emission from landfills. Various physico-chemical and environmental variables like proportion of amended biocover material (rice husk), temperature, moisture content, CH4 concentration, CO2 concentration, O2 concentration and incubation time were considered in the study which affect the CH4 bio-oxidation. For the present study, sequential statistical approach with Placket Burman Design (PBD) was used to identify significant variables, having influential role on CH4 bio-oxidation, from all variables. Further, interactive effect of four selected variables including rice husk proportion, temperature, CH4 concentration and incubation time was studied with Box-Behnken Design (BBD) adopting Response Surface Methodology (RSM) to optimize the conditions for CH4 oxidation. In this study, the maximum CH4 oxidation potential of 76.83µgCH4g(-1)dwh(-1) was observed under optimum conditions with rice husk amendment of 6% (w/w), 5h incubation time at 40°C temperature with 40% (v/v) initial CH4 concentration. The results for CH4 oxidation potential also advocated the suitability of rice husk amendment in biocover system to curb emitted CH4 from landfills/open dumpsite over conventional clay or sand cover on supplying CH4 and O2 to microbes on maintaining proper aeration.

Power generation in microbial fuel cell fed with post methanation distillery effluent as a function of pH microenvironment.

The effect of anolyte and catholyte pH on power generation in an MFC using post methanation distillery effluent (PMDE) was studied in batch mode. Higher anodic pH (7-9) and low cathodic pH (2) were more favorable and at the optimal cathode:anode pH ratio of 2:8, power density attained was 0.457 W/m(3). An initial feed solution pH up to 10 was tolerated by the MFC. However, internal resistance increased 1.5 times and power density decreased by 60% at pH 10 as compared to that at pH 7, the normal anolyte pH. Internal resistance of the MFC was minimum (266 ohms) at cathodic pH 2, thus favoring better power generation. Under low cathodic and high anodic pH ratio of the MFC, a low internal resistance favored both high current density and power density.

Integrating photobiological hydrogen production with dye-metal bioremoval from simulated textile wastewater.

The study reports production of hydrogen in photobioreactors with free (PBR(Fr)) and immobilized (PBR(Imm)) Nostoc biomass at enhanced and sustained rates. Before running the photobioreactors, effects of different immobilization matrices and cyanobacterial dose on hydrogen production were studied in batch mode. As hydrogen production in the PBRs declined spent biomass from the photobioreactors were collected and utilized further for column biosorption of highly toxic dyes (Reactive Red 198+Crystal Violet) and metals (hexavalent chromium and bivalent cobalt) from simulated textile wastewater. Breakthrough time, adsorption capacity and exhaustion time of the biosorption column were studied. The photobioreactors with free and immobilized cyanobacterium produced hydrogen at average rates of 101 and 151 µmol/h/mg Chl a, respectively over 15 days, while the adsorption capacity of the spent biomass was up to 1.4 and 0.23 mg/g for metals and 15 and 1.75 mg/g for the dyes, respectively in continuous column mode.

Assessment of heavy metal tolerance in native plant species from soils contaminated with electroplating effluent.

Heavy metals concentrations of (Cr, Zn, Fe, Cu and Ni) were determined in plants and soils contaminated with electroplating industrial effluent. The ranges of total soil Cr, Zn, Fe, Cu and Ni concentrations were found to be 1443-3240, 1376-3112, 683-2228, 263-374 and 234-335 mg kg⁻¹, respectively. Metal accumulation, along with hyperaccumulative characteristics of the screened plants was investigated. Present study highlighted that metal accumulation in different plants varied with species, tissues and metals. Only one plant (Amaranthus viridis) accumulated Fe concentrations over 1000 mg kg⁻¹. On the basis of TF, eight plant species for Zn and Fe, three plant species for Cu and two plant species for Ni, could be used in phytoextraction technology. Although BAF of all plant species was lesser than one, these species exhibited high metal adaptability and could be considered as potential hyperaccumulators. Phytoremediation potential of these plants can be used to remediate metal contaminated soils, though further investigation is still needed.

Changing patterns of organochlorine pesticide residues in raw bovine milk from Haryana, India.

Bovine milk samples were collected and analyzed during 1992 and 1998 from rural areas of 14 different districts of Haryana state for the presence of HCH and DDT residues. The study revealed that the mean residues of ΣHCH in raw bovine milk have declined by 67.5% while mean levels of ΣDDT have decreased by 92.8% during six years gap. The obtained results reveal that during 1992 p,p'-DDT was the main component followed by p,p'-DDD, α-HCH and ß-HCH while in 1998, p,p'-DDE and ß-HCH followed by p,p'-DDT were relatively more as compared to other isomers and metabolites of these pesticides.

Hydrogen production and metal-dye bioremoval by a Nostoc linckia strain isolated from textile mill oxidation pond.

Biohydrogen production by Nostoc linckia HA-46, isolated from a textile-industry oxidation-pond was studied by varying light/dark period, pH, temperature and ratio of carbon-dioxide and argon in the gas-mixture. Hydrogen production rates were maximum under 18 h of light and 6 h of darkness, pH 8.0, 31°C, a CO(2):Ar ratio 2:10. Hydrogen production of the strain acclimatized to 20 mg/L of chromium/cobalt and 100 mg/L of Reactive red 198/crystal violet dye studied in N-supplemented/deficient medium was 6-10% higher in the presence of 1.5 g/L of NaNO(3). Rates of hydrogen production in the presence of dyes/metals by the strain (93-105 µmol/h/mg Chlorophyll) were significantly higher than in medium without metals/dyes serving as control (91.3 µmol/h/mg Chlorophyll). About 58-60% of the two metals and 35-73% of dyes were removed by cyanobacterium. Optimal conditions of temperature, pH and metals/dyes concentration for achieving high hydrogen production and wastewater treatment were found practically applicable as similar conditions are found in the effluent of regional textile-mills.

Chromium (VI) tolerance in two halotolerant strains of Nostoc.

The present study reports on chromium (VI) tolerance of two cyanobacterial strains Nostoc linckia and Nostoc spongiaeforme isolated from salt affected soils using uni-algal and bi-algal systems. Besides distinct halophilism, the two strains exhibited remarkable tolerance to chromium (VI) and revealed 1.2 to 2.8 times more chlorophyll in the presence of the metal. While phycobilins and carotenoids also increased in Nostoc linckia with total dissolved salts (TDS) as well as metal, a decline was observed in Nostoc spongiaeforme in the presence of Cr (VI). Relative algal biomass (as % of control) showed significantly higher values (123-239) in Nostoc linckia in the presence of salt, metal and combination of the two. In Nostoc spongiaeforme it declined in the presence of metal (72-81) but increased in the presence of salts (143-249) and also in the binary systems (121-440). The bi-algal consortium showed relatively less tolerance to salt and metal stress. Nostoc linckia (20 day culture) showed upto 40% chromium removal whereas Nostoc spongiaeforme showed up to 12% removal, indicating greater suitability of the former for use in bioremediation studies.

Sequestration of chromium by exopolysaccharides of Nostoc and Gloeocapsa from dilute aqueous solutions.

This article reports the chromium removal potential of exopolysaccharides (EPS) of two indigenously isolated cyanobacterial strains, Gloeocapsa calcarea and Nostoc punctiforme. The biosorption was studied by varying pH from 2 to 6 and initial chromium concentration from 5 to 20mg/L to find out the optimized conditions for maximum chromium removal by EPS. Two equilibrium models, Langmuir and Freundlich, were used to explain these results. The Freundlich model was found to be better applicable to the experimental data as compared to Langmuir as inferred from high value of coefficient of determination whereas the optimal conditions were found to be same for the two (pH 2 and initial chromium concentration 20 mg/L). EPS production by the two strains was also studied which was found to be higher for Gloeocapsa. On the basis of experimental results and model parameters, it can be inferred that the EPS extracted from Nostoc has comparatively high biosorption capacity and can be utilized for the removal of chromium from dilute aqueous solution. Adsorption of chromium on EPS was further confirmed by surface morphology observed in scanning electron micrographs.

Biosorption of Cr(VI) by immobilized biomass of two indigenous strains of cyanobacteria isolated from metal contaminated soil.

Biosorption of Cr(VI) using native strains of cyanobacteria from metal contaminated soil in the premises of textile mill has been reported in this paper. Biosorption was studied as a function of pH (1-5), contact time (5-180 min) and initial chromium ion concentration (5-20mg/l) to find out the maximum biosorption capacity of alginate immobilized Nostoc calcicola HH-12 and Chroococcus sp. HH-11. The optimum conditions for Cr(VI) biosorption are almost same for the two strains (pH 3-4, contact time 30 min and initial chromium concentration of 20mg/l) however, the biomass of Chroococcus sp. HH-11 was found to be more suitable for the development of an efficient biosorbent for the removal of Cr(VI) from wastewater, as it showed higher values of q(m) and K(f), the Langmuir and Freundlich isotherm parameters. Both the isotherm models were suitable for describing the biosorption of Cr(VI) by the cyanobacterial biosorbents.

Biosorption of Cr(VI) by native isolate of Lyngbya putealis (HH-15) in the presence of salts.

Industrial wastewaters containing heavy metals along with high concentration of soluble salts pose a major environmental problem that needs to be remedied. The present study reports on biosorption of Cr(VI) by native isolate of Lyngbya putealis HH-15 in batch system under varying range of pH (2.0-10.0), initial metal ion concentration (10-100mg/l) and salt concentration (0-0.2%). Maximum metal removal (94.8%) took place at pH 3.0 with initial Cr concentration of 50mg/l, which got reduced (90.1%) in the presence of 0.2% salts. Adsorption equilibrium and kinetic behavior of Cr(VI) in solution was also examined. Both Langmuir and Freundlich models fitted well to explain the adsorption data (R(2)=0.90 and 0.87, respectively) at 0.2% salt concentration. Pseudo-second order kinetic model also fitted well to both the systems, viz. Cr(VI) and Cr(VI)+salt.

Seasonal variations in physico-chemical characteristics of River Yamuna in Haryana and its ecological best-designated use.

Various physico-chemical characteristics of the River Yamuna flowing in Haryana through Delhi were studied in the summer (April 1998) and winter (Jan.-Feb. 1999). Ecological parameters like dissolved oxygen (DO), pH, nitrate (NO3-), sulfate (SO4(2-)), and phosphate (PO4(3-)), were analyzed and compared with standard permissible limits to assess the best-designated use of the river water for various purposes. The river in Delhi upstream was of better quality whereas the Delhi downstream stretch was polluted as indicated by very low DO and high total dissolved solids (TDS), electric conductivity (EC), total hardness, Na+, K+, Cl-, F- and SO4(2-). The differences in various parameters were statistically significant (p < 0.01) when compared for the Delhi upstream and downstream stretches of the river, particularly in summer. DO and TDS were found to be two important parameters, which showed strong correlation with several other parameters and hence can serve as good indices of river water quality. The river tended to recover from the pollution stress after flowing through a distance of about 80 km downstream of Delhi.