RESUMEN
The results of the correction of disturbances in intestinal microflora in elderly patients with the use of bifidumbacterin in the form of powder or suppositories are presented. The effectiveness of the rectal administration of bifidumbacterin (by microinjections through an enema or in suppositories) is shown in comparison with the oral administration of the preparation. The administration of bifidumbacterin in suppositories for 15 days resulted in decreased content of hemolytic Escherichia below the threshold of determination in all examined patients.
Asunto(s)
Antiinfecciosos/uso terapéutico , Productos Biológicos/uso terapéutico , Enfermedades Intestinales/terapia , Administración Oral , Administración Rectal , Anciano , Antiinfecciosos/administración & dosificación , Productos Biológicos/administración & dosificación , Recuento de Colonia Microbiana , Enema , Escherichia coli O157/aislamiento & purificación , Femenino , Humanos , Enfermedades Intestinales/microbiología , Masculino , Persona de Mediana Edad , Supositorios/administración & dosificaciónRESUMEN
A method for measuring urease activity in biopsy specimens and Helicobacter pylori cultures from these specimens is proposed. The method is based on measurement (with a portable pH-meter) of the rate of pH changes in a reaction mixture consisting of buffer, substrate (urea), and biopsy specimen or bacterial cells. This method revealed that urease activity of biopsy specimens correlated with that of H. pylori suspension in the same experiment. High urease activity was found in biopsy specimens containing the greatest number of Helicobacter cells; only one of 14 specimens free of H. pylori cells showed no urease activity. Introduction of this method into clinical practice will help to evaluate the contribution of H. pylori to the pathological process.
Asunto(s)
Mucosa Gástrica/enzimología , Helicobacter pylori/enzimología , Ureasa/metabolismo , Adulto , Anciano , Biopsia , Femenino , Humanos , Concentración de Iones de Hidrógeno , Masculino , Persona de Mediana EdadRESUMEN
The bacteriological analysis of 302 children has revealed that dysbacteriosis with the increased content of hemolytic Escherichia in the intestine develops more frequently in the presence of the deficiency of bifido- and lactobacteria. The development of other kinds of dysbacteriosis with the increased content of different opportunistic enterobacteria, staphylococci, enterococci and fungi, as well as dysbacteriosis with the decreased content of Escherichia, does not practically depend on the deficiency of bifido- and lactobacteria. In patients with the increased content of Escherichia an increase in the content of opportunistic enterobacteria, staphylococci and fungi is observed more frequently than in patients with the low content of Escherichia.
Asunto(s)
Infecciones Bacterianas/microbiología , Enfermedades Intestinales/microbiología , Población Urbana , Adulto , Bacterias/aislamiento & purificación , Niño , Recuento de Colonia Microbiana , Heces/microbiología , Humanos , MoscúRESUMEN
The antigen-specific activity of polycomponent vaccine VP-4 consisting of Klebsiella pneumoniae, Staphylococcus aureus, Proteus vulgaris and Escherichia coli antigens was studied. The same form of the vaccine was used for both subcutaneous injection and oral administration. The oral administration of the preparation to rabbits and guinea pigs induced a rise in the level of antibodies to all components of the vaccine in their blood sera, the optimum oral doses exceed only 2- to 4-fold the doses used for subcutaneous injection. The form of vaccine VP-4, developed for subcutaneous injection, may be recommended for wider trials with its oral administration.
Asunto(s)
Antígenos Bacterianos/administración & dosificación , Vacunas Bacterianas/administración & dosificación , Epítopos/inmunología , Escherichia coli/inmunología , Klebsiella pneumoniae/inmunología , Proteus vulgaris/inmunología , Staphylococcus aureus/inmunología , Administración Oral , Animales , Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/inmunología , Vacunas Bacterianas/inmunología , Cobayas , Inmunización/métodos , Inyecciones Subcutáneas , Conejos , Vacunas CombinadasRESUMEN
It was shown in experiments with mice and dogs that bacterial preparations (vaccinum proteus, prodigiosanum, bificolum and bificolum-f) administered before or after gamma-irradiation have a stimulating effect on hemopoiesis. It is supposed that bacterial polysaccharides play an important role in this action.
Asunto(s)
Productos Biológicos/uso terapéutico , Hematopoyesis/efectos de los fármacos , Traumatismos Experimentales por Radiación/terapia , Enfermedad Aguda , Animales , Vacunas Bacterianas/uso terapéutico , Bacteriocinas/uso terapéutico , Perros , Relación Dosis-Respuesta en la Radiación , Ratones , Prodigiozán/uso terapéutico , Proteus/inmunología , Traumatismos Experimentales por Radiación/sangre , Factores de TiempoAsunto(s)
Antígenos Bacterianos/inmunología , Antígenos Bacterianos/aislamiento & purificación , Salmonella typhi/inmunología , Animales , Hidroxilaminas , Inmunización/métodos , Indicadores y Reactivos , Inyecciones Intraperitoneales , Ratones , Vacunas Tifoides-Paratifoides/inmunología , Vacunas Tifoides-Paratifoides/aislamiento & purificaciónAsunto(s)
Proteínas de la Membrana Bacteriana Externa/análisis , Proteínas Bacterianas , Epítopos/análisis , Hierro/análisis , Neisseria meningitidis/química , Animales , Anticuerpos Antibacterianos/sangre , Especificidad de Anticuerpos , Proteínas de la Membrana Bacteriana Externa/inmunología , Epítopos/inmunología , Inmunización , Immunoblotting/métodos , Hierro/inmunología , Proteínas de Unión a Hierro , Peso Molecular , Neisseria meningitidis/inmunología , Proteínas de Unión Periplasmáticas , ConejosRESUMEN
Multicomponent vaccine VP-4, prepared from Streptococcus, Klebsiella, Proteus and Escherichia coli antigens, was tested for protective activity by oral administration to mice with experimental Klebsiella, Proteus and S. pneumoniae infections. Vaccine VP-4, when introduced orally (in 2-3 administrations of 2 mg), was found to produce a protective effect with respect to K. pneumoniae (the effectiveness index was equal to 5.7 and 4.3). With respect to P. vulgaris, the effectiveness index was 2.7 (immunization was made in two oral administrations of 2 mg); in pneumococcal infection preliminary oral immunization with vaccine VP-4 in two administrations in a dose of 2-4 mg produced high protective effect. Thus, immunization with vaccine VP-4, introduced orally in comparatively small doses, proved to be effective in animal models used in the experiment.
Asunto(s)
Vacunas Bacterianas/inmunología , Escherichia coli/inmunología , Klebsiella pneumoniae/inmunología , Proteus vulgaris/inmunología , Staphylococcus aureus/inmunología , Vacunas Combinadas/inmunología , Administración Oral , Animales , Vacunas Bacterianas/administración & dosificación , Vacunas Bacterianas/toxicidad , Relación Dosis-Respuesta Inmunológica , Evaluación Preclínica de Medicamentos , Inmunización , Infecciones por Klebsiella/prevención & control , Dosificación Letal Mediana , Ratones , Infecciones Neumocócicas/prevención & control , Infecciones por Proteus/prevención & control , Vacunas Combinadas/administración & dosificación , Vacunas Combinadas/toxicidadRESUMEN
The results of the study of the antigenic activity of multicomponent vaccine consisting of staphylococcal, Klebsiella, Proteus, Escherichia coli antigens and staphylococcal monovaccine, introduced into rabbits and guinea pigs by different routes, are presented. As shown in this study, the multicomponent vaccine introduced orally in 5 administrations stimulated the production of antibodies to all components, but the intensity of antibody formation to each of them was different. Antibodies to E. coli antigen were found to be at the lowest level. Staphylococcal antigen, introduced orally both as the component of the multicomponent vaccine and as the monovaccine, ensured pronounced stimulation of the production of antistaphylococcal antibodies, not lower than that observed after subcutaneous injection. The comparative study of the activity of the multicomponent vaccine introduced by combined routes, oral-subcutaneous and subcutaneous-oral, showed the advantage of the former one.
Asunto(s)
Antígenos Bacterianos/administración & dosificación , Vacunas Bacterianas/administración & dosificación , Inmunización/métodos , Administración Oral , Animales , Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/inmunología , Vacunas Bacterianas/inmunología , Combinación de Medicamentos , Escherichia coli/inmunología , Cobayas , Inyecciones Subcutáneas , Klebsiella pneumoniae/inmunología , Proteus vulgaris/inmunología , Conejos , Vacunas Estafilocócicas/administración & dosificación , Vacunas Estafilocócicas/inmunología , Staphylococcus aureus/inmunología , Factores de TiempoRESUMEN
Multicomponent vaccine prepared from the antigens of 4 representatives of opportunistic microflora possesses high specific activity. The passive hemagglutination (PHA) test with the use of associated diagnosticum showed that antibody titers in the sera of immunized rabbits increased 10- to 10(4)-fold in comparison with the titers observed prior to immunization. The PHA test with the use of the antigens contained in the vaccine revealed the accumulation of antibodies to each of the 4 components of the preparation in the blood sera of immunized rabbits. When stored at 4 degrees C, the vaccine was shown to retain its specific activity for 5 years (the term of observation).
Asunto(s)
Anticuerpos Antibacterianos/sangre , Especificidad de Anticuerpos/inmunología , Antígenos Bacterianos/inmunología , Vacunas Bacterianas/inmunología , Escherichia coli/inmunología , Klebsiella pneumoniae/inmunología , Proteus vulgaris/inmunología , Staphylococcus aureus/inmunología , Animales , Pruebas de Hemaglutinación , Inmunización , Conejos , Factores de TiempoRESUMEN
As early as the first 24 hours since a severe isolated locomotor trauma the patients were immunized with Proteus vaccine. Such vaccination is shown to stimulate the production of serum specific antibodies, to reduce contamination of the wound with gram-negative agents, to shorten hospital stay and healing of the wound versus subjects vaccinated with staphylococcal anatoxin and nonimmunized patients.
Asunto(s)
Vacunas Bacterianas/administración & dosificación , Proteus/inmunología , Heridas y Lesiones/terapia , Vacunas Bacterianas/inmunología , Servicio de Urgencia en Hospital , Estudios de Evaluación como Asunto , Femenino , Humanos , Inmunización , Masculino , Moscú , Vacunas Estafilocócicas/administración & dosificación , Infección de Heridas/inmunología , Infección de Heridas/prevención & control , Heridas y Lesiones/inmunologíaRESUMEN
Klebsiella vaccine, when injected subcutaneously to donors, proved to be faintly reactogenic and safe. The injection of the vaccine had no effect on changes in the morphological composition of peripheral blood and on liver function. In persons with the initially low content of IgG an increase in this characteristic was observed after immunization. No changes in the synthesis of IgE occurred in healthy donors under the influence of immunization. The vaccine was shown to be immunogenic when introduced according to immunization schedules comprising 3 and 5 injections, the titer of Klebsiella antibodies increasing 3- to 5-fold.
Asunto(s)
Vacunas Bacterianas/efectos adversos , Donantes de Sangre , Klebsiella pneumoniae/inmunología , Adulto , Anticuerpos Antibacterianos/sangre , Vacunas Bacterianas/inmunología , Evaluación de Medicamentos , Femenino , Humanos , Esquemas de Inmunización , Inmunoglobulinas/análisis , Inyecciones Subcutáneas , Masculino , Persona de Mediana Edad , Factores de TiempoRESUMEN
The comparative study of the chemical composition and biological properties of antigens isolated from Proteus vulgaris with the use of hydroxylamine and by two classical methods (Boivin's and Westphal's methods) has been made. As shown in this study, the treatment of bacteria with hydroxylamine makes it possible to obtain antigenic complexes with lower toxicity. At the same time hydroxylamine produces no denaturing effect on lipopolysaccharides and protein fractions of bacterial cells.
Asunto(s)
Antígenos Bacterianos/análisis , Hidroxilaminas , Proteus vulgaris/inmunología , Animales , Antígenos Bacterianos/inmunología , Antígenos Bacterianos/aislamiento & purificación , Antígenos Bacterianos/toxicidad , Fenómenos Químicos , Química , Hidroxilamina , Inmunización , Técnicas Inmunológicas , Indicadores y Reactivos , Dosificación Letal Mediana , Lipopolisacáridos/análisis , Lipopolisacáridos/inmunología , Lipopolisacáridos/aislamiento & purificación , Lipopolisacáridos/toxicidad , Ratones , Proteus vulgaris/efectos de los fármacosRESUMEN
The data on the study of the reactogenicity, safety and prophylactic potency of a new acellular vaccine prepared from S. flexneri 2a antigenic complexes are presented. According to the results of two epidemic experiments, the vaccine, introduced by oral administration, showed low reactogenicity, safety and sufficient prophylactic potency. The vaccine decreased morbidity rate in dysentery caused by S. flexneri 2a and ensured the protection of 74% (72-80%) of the vaccinees. The complete course of immunization consisting of three administrations followed by the booster administration induces the formation of specific immunity whose duration is sufficient for ensuring the protection of immunized persons during the epidemiologically unfavorable period (for at least 3 months).
Asunto(s)
Vacunas Bacterianas/administración & dosificación , Shigella flexneri/inmunología , Administración Oral , Adolescente , Adulto , Antígenos Bacterianos/inmunología , Vacunas Bacterianas/efectos adversos , Vacunas Bacterianas/inmunología , Relación Dosis-Respuesta Inmunológica , Evaluación de Medicamentos , Disentería Bacilar/epidemiología , Disentería Bacilar/prevención & control , Humanos , Masculino , Comprimidos , U.R.S.S./epidemiologíaAsunto(s)
Amputación Traumática/terapia , Vacunas Bacterianas/uso terapéutico , Fracturas Abiertas/terapia , Proteus/inmunología , Toxoide Estafilocócico/uso terapéutico , Infección de Heridas/prevención & control , Adolescente , Adulto , Amputación Traumática/complicaciones , Amputación Traumática/inmunología , Terapia Combinada , Evaluación de Medicamentos , Femenino , Fracturas Abiertas/complicaciones , Fracturas Abiertas/inmunología , Humanos , Inmunización/métodos , Masculino , Persona de Mediana Edad , Infección de Heridas/inmunologíaRESUMEN
Highly sensitive and specific erythrocyte diagnostic agents (ED) for the determination of antibodies to Proteus O-antigens have been obtained by the sensitization of formolated sheep red blood cells (SPBC) with activated lipopolysaccharides (LPS) without the use of mediators. The tannin treatment of formolated SRBC and/or the increase of temperature from 45 degrees C to 100 degrees C in the process of the preparation of ED have been found to produce no increase in effectiveness. Antibody ED permitting the detection of Proteus O- and H-antigens has been obtained by the sensitization of formolated chick red blood cells with immunoglobulin preparations to Proteus hydroxylamine antigens, carried out with the use of amidol. The experiments have shown the possibility of using this antibody ED for the determination of O-antibodies in the antigen neutralization test with nonactivated LPS used as an agglutinating agent. The passive hemagglutination test with antibody ED has proved to be a more sensitive method for the detection of O-antibodies than the antigen neutralization test with antigenic ED. The determination of Proteus etiology in the passive hemagglutination test with the use of antigenic ED has been shown to be highly effective in the examination of patients with chronic osteomyelitis at the stage of exacerbation.
Asunto(s)
Anticuerpos Antibacterianos/análisis , Antígenos Bacterianos/inmunología , Eritrocitos/inmunología , Proteus/inmunología , Animales , Especificidad de Anticuerpos , Enfermedad Crónica , Pruebas de Hemaglutinación , Humanos , Sueros Inmunes/inmunología , Sueros Inmunes/aislamiento & purificación , Inmunización , Pruebas de Neutralización , Antígenos O , Osteomielitis/inmunología , ConejosRESUMEN
S. aureus aqueous extract and K. pneumoniae hydroxylamine vaccine were studied by means of chemical and immunochemical analytical techniques. The preparations were found to contain, respectively, 7.0% nad 53.5% of neutral monosaccharides, 6.5% and 0.7% of nucleic acids, as well as protein in approximately equal amounts (11.63-14.0%). In experiment of immunodiffusion, immunoelectrophoresis and rocket immunoelectrophoresis in homologous systems with hyperimmune antimicrobial sera the preparations were characterized by serological heterogeneity. After their combination with Escherichia coli aqueous extract and Proteus hydroxylamine preparation their serological characteristics remaIned unchanged. The study of cross reactions of all components of the combined preparations with hyperimmune rabbit sera to the corresponding microorganisms revealed that only Klebsiella component of the combined vaccine reacted with all hyperimmune sera. The preparation of Proteus showed the lowest activity, it reacted only with hyperimmune sera to K. pneumoniae. Besides, no reaction of S. aureus component with sera to E. coli and no reaction of the preparation of E. coli with antistaphylococcal serum were observed.
Asunto(s)
Antígenos Bacterianos/análisis , Vacunas Bacterianas/análisis , Klebsiella pneumoniae/inmunología , Staphylococcus aureus/inmunología , Antígenos Bacterianos/inmunología , Vacunas Bacterianas/inmunología , Fenómenos Químicos , Química , Reacciones Cruzadas , Combinación de Medicamentos , Escherichia coli/inmunología , Sueros Inmunes/inmunología , Inmunoquímica , Inmunodifusión , Inmunoelectroforesis , Proteus vulgaris/inmunologíaRESUMEN
The results of the study of rabbit antisera to meningococci A, B, C in the double diffusion in gel, passive hemagglutination test and enzyme immunoassay with antigenic preparations isolated from S. aureus strains are indicative of the presence of common antigenic determinants of protein and polysaccharide nature in S. aureus and N. meningitidis.
Asunto(s)
Anticuerpos Antibacterianos/análisis , Antígenos Bacterianos/análisis , Neisseria meningitidis/inmunología , Staphylococcus aureus/inmunología , Animales , Especificidad de Anticuerpos , Reacciones Cruzadas , Epítopos/análisis , Pruebas de Hemaglutinación , Inmunodifusión , Técnicas para Inmunoenzimas , Neisseria meningitidis/clasificación , Conejos , SerotipificaciónRESUMEN
The comparative study of the immunological activity of Proteus vaccine prepared from soluble antigenic complexes was made after the immunization of volunteers with this vaccine used in the form of a single preparation and in combination with pyoimmunogen (Pseudomonas aeruginosa vaccine) and/or adsorbed staphylococcal toxoid. The injection of the vaccine in the form of a single preparation and in different combinations increased the ingestion of Proteus cells by neutrophils. The injection of Proteus vaccine simultaneously with pyoimmunogen and staphylococcal toxoid ensured the intensive phagocytosis of staphylococci. All combinations with Proteus vaccine, used in this investigation, stimulated the intensive formation of antibodies to Proteus vaccine strain and Re-glycolipid. Proteus vaccine introduced in combination with adsorbed staphylococcal toxoid essentially stimulated the synthesis of anti-alpha-staphylolysin.