RESUMEN
The efficacy of the methanolic fraction (MF) of Tribulus terrestris fruit extract on mercury intoxicated mice, Mus musculus has been studied. At a median-lethal dose of mercuric chloride (12.9 mg/kg body wt.) administration an enhanced level of glutamate oxaloacete transaminase (GOT), glutamate pyruvate transminase (GPT) and simultaneously decreased level of acid phosphatase (ACP) and alkaline phosphatase (ALT) activities were noticed in the liver. Due to the mercury toxicity the liver cells are damaged to cause the alterations in their enzymes. During the recovery period, all the enzymological parameters are restored to reach near normal level. The result suggested that the oral administration of MF of T. terrestris fruit extract has (6 mg/kg body wt.) provided protection against the mercuric chloride induced hepatic damage in the mice, M. musculus.
Asunto(s)
Hígado/efectos de los fármacos , Cloruro de Mercurio/envenenamiento , Extractos Vegetales/uso terapéutico , Tribulus , Fosfatasa Ácida/metabolismo , Alanina Transaminasa/metabolismo , Fosfatasa Alcalina/metabolismo , Animales , Aspartato Aminotransferasas/metabolismo , Femenino , Hígado/enzimología , RatonesRESUMEN
The present study investigates the influence of methanolic fraction (MF) of Tribulus terrestris fruit extract on the kidney tissues of mercury intoxicated mice, Mus musculus. At median-lethal dose of mercuric chloride (12.9 mg/kg body weight), the whole kidney tissue showed an increased level of lipid peroxidation (LPO), glutathione peroxidase (GPx) activities and simultaneously a decreased level of superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH). These parameters reached to near normal after administration of fruit extracts of T. terrestris for 7 days. The results suggested that the oral administration of methanolic fraction of Tribulus terrestris fruit extract at dose 6 mg/kg body weight provided protection against the mercuric chloride induced toxicity in the mice.
Asunto(s)
Riñón/efectos de los fármacos , Cloruro de Mercurio/toxicidad , Extractos Vegetales/farmacología , Zygophyllaceae/química , Animales , Catalasa/metabolismo , Femenino , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Dosificación Letal Mediana , Peroxidación de Lípido , Ratones , Superóxido Dismutasa/metabolismoRESUMEN
FT-IR spectra of liver tissue isolated from mice, Mus musculus, have been recorded in the region of 4000 - 400 cm-1 for normal, mercury treated and recovery phase. In this study, the total protein content was found to be decreased in the liver tissues after treatment with median-lethal dose of mercuric chloride. The marked fall in the level of bio-chemical constituent in the tissue due to metal exposure indicates the rapid initiation of the breakdown of the bio-chemical constituents to meet the energy demand during toxic stress. During the recovery phase, the decreased levels of bio-chemical constituents are restored to near normal level. Methanol fractions of Tribulus terrestris fruit extract was administrated on mercury intoxicated mice for 15 days. After the administration, the mercury-intoxicated animals slowly recovered from the adverse effect of mercury poisoning with the help of plant bio-formulations. The results are discussed in detail.