Determination of dysbiosis by the method of multiplex real-time polymerase chain reaction in chronic catarrhal gingivitis in children.

OBJECTIVE: Aim: The aim of the study was to determine the quantitative and qualitative characteristics of the microbiota of dento-gingival plaque in children to improve the quality of treatment of chronic catarrhal gingivitis. PATIENTS AND METHODS: Materials and Methods: It was examined 16 children aged 9-16 years with a diagnosis of K05.1: chronic gingivitis and 10 persons with intact gums were taken as a comparison group. A clinical dental examination was performed on the study participants and a sample was taken to determine the bacteria in the periodontal plaque. RESULTS: Results: The results of statistical processing of the research data allowed us to establish that in patients with chronic gingivitis, quantitative indicators of the total bacterial mass, Lactobacillus spp., Enterobacteriaceae, Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp. in the sample of periodontal plaque significantly exceeded the indicators of healthy patients. It was determined that the examined children with chronic gingivitis, the total number of Lactobacillus spp. significantly exceeds its amount in people with intact gums. CONCLUSION: Conclusions: The changes in the quantitative and qualitative characteristics of the main representatives of the microf i lm of dento-gingival plaque, which characterize dysbiosis, are of signif i cant clinical signif i cance. Study of the quantitative characteristics of Lactobacterium spp., Enterobacterium spp., Streptococcacea spp., Gardnerella spp., Prevotella spp., Porphyromonas spp., Eubacteridacea spp., Mycoplasma (hominis + genitalium), Candida spp. is a diagnostic factor in determining the condition of the mucous membrane of the oral cavity.

CYTOLOGIC CHARACTERISTIC OF THE CELLULAR COMPOSITION OF THE GUM MUCOUS MEMBRANE IN SCHOOL-AGE CHILDREN.

OBJECTIVE: The aim: To establish the characteristics of the gingival mucosa cellular composition in school-age children. PATIENTS AND METHODS: Materials and methods: We made a dental and cytological examination on 150 Ukrainian (Poltava city) children aged 6, 12, and 15 years. Smears were got by scraping from the gum mucosa and stained with a May-Grunwald solution. Cytograms were made by using a Biorex-3 BM-500T microscope with a DCM-900 digital microphotographic attachment. The programs were adapted for these types of studies. We used a magnification of 1000. The t-test for paired samples was used to compare values. The difference was considered statistically significant at P<0.05. RESULTS: Results: The number of superficial epitheliocytes in children aged 12 and 15 was significantly higher than in 6-year-olds but did not differ from each other. CONCLUSION: Conclusions: The process of physiological keratinization of the cells of the gingival mucosa in schoolchildren is diverse and keratinization occurs both due to the phenomenon of physiological necrosis, namely, apoptosis of surface cells and due to orthokeratosis. From the age of 6, the average number of surface cells in cytograms decreases, while the number of intermediate and dead keratinized cells increases up to 12 years and remains on the previous level in children of 15 years.

AFFECTION ON CARIES AND ITS COMPLICATIONS OF TEMPORARY TEETH OF CHILDREN IN A REGION WITH EXCESS FLUORINE CONTENT IN DRINKING WATER.

OBJECTIVE: Aim: The aim of the study was to determine the indicators of caries and its complications in the temporary teeth of children who permanently live in a region with a high fluoride content in drinking water. PATIENTS AND METHODS: Materials and Methods: It was examined with the definition of caries and its complications 277 children in the age range from 2 to 13 years, who were born and permanently live in the urban-type settlement of Mashivka. The fluoride content in the drinking water of the settlement was 1.7-2.5 mg/l. RESULTS: Results: During the analysis of data from the survey of children who were born and permanently lived in the urban-type settlement of Mashivka, it was determined that the prevalence of caries of temporary teeth probably increases with age. Half of the 3-5-year-old children had caries-affected teeth, and temporary tooth caries reached the highest rates in 10-year-old children. It should be noted that a fifth of children in the youngest age group (3-5 years old) suffer from pulpitis and periodontitis of temporary teeth. CONCLUSION: Conclusions: The conducted examination of children urban-type settlement of Mashivka confirms the opinion that the excessive content of fluorine in drinking water does not have a caries-protective effect, and the intensity of the process reaches the indicators characteristic of regions with its optimal content. Such a situation requires strengthening measures for both primary and secondary prevention of dental diseases.

Morphometric description of the exchange segment of microvasculature of rats' salivary glands in normal conditions and chronic ethanol intoxication.

OBJECTIVE: Introduction: The state of the segments of the microvasculature, especially exchange one, has a significant effect on the organs' functions. The results of it's research can be objectified by morphometric method. The aim: to determine the dynamics of changes in metric indices of the exchange segment of microvasculature of rats' submandibular glands in normal conditions and in chronic ethanol intoxication. PATIENTS AND METHODS: Materials and methods: 45 albino rats were involved into study. Intact group (n=5) animals were administered with NaCl isotonic solution 4 times a day directly into the stomach. Experimental group (n=40) animals were administered with 40° ethanol 4 times a day directly into the stomach. Animals were sacrificed on 5, 9, 12 and 30 days by overdose of thiopental anesthesia. Lobules of submandibular glands were embedded into epon-812 according to standard procedure. RESULTS: Results: On day 5 of the experiment the outer diameter of the capillary wall significantly reduced (4,91±0,02 µm), that is 19,5 % less than the values in controls (р<0,05). On day 9 of the experiment it was 15,2 % lower than the value of control group (р>0,05). On day 30 of the experiment the outer capillary diameter was 8 % lower compared to controls (р<0,05). CONCLUSION: Conclusions: The early observation showed vasodilatation, confirmed by the constriction of the outer diameter and lumen diameter, accompanied by the thickening of the vascular wall under the influence of chronic ethanol intoxication. The indices did not come to normal values by the 30-th day of the experiment.

Monitoring of efficiency of dental caries management in children's temporary teeth throughout Poltava oblast.

OBJECTIVE: Introduction: Dental caries is still a major health care problem not only in Ukraine but throughout the world. Complicated dental caries currently ranks the leading position among the oral diseases in children. The aim of this study is to carry out a analysis of the epidemiology of dental caries and its complications, and to evaluate the efficiency of the caries treatment for temporary teeth among the children of Poltava oblast over the last decade based on the data of the annual reports. PATIENTS AND METHODS: Materials and methods: We have studied the figures reflecting the epidemiology of dental caries and its complications in Poltava oblast over the past ten years obtained through the analysis of the annual reports on the quantitative and qualitative indicators of paediatric dental service. RESULTS: Review: According to the annual reports from the districts, the number of cases of temporary teeth treatment for complicated caries did not change significantly when comparing the relevant data of 2007 and 2017. When carrying out the comparative analysis of annual reports for 2007 and 2017 by the indicator of the share of complicated caries, attention should be focused on the average increase of the value from 30.4% in 2007 to 35.9% in 2017. CONCLUSION: Conclusions: The analysis of annual reports for 2007 and 2017 through Poltava oblast points out the low efficiency of dental caries management in the children's temporary teeth. As a result, outcomes of the dental caries treatment and caries complications in the children's temporary teeth are assessed as unsatisfactory both in the preventive and the therapeutic aspects.