Spotlight on therapeutic efficiency of green synthesis metals and their oxide nanoparticles in periodontitis.

Periodontitis, one of the most prevalent dental diseases, causes the loss of bone and gum tissue that hold teeth in place. Several bacteria, commonly present in clinically healthy oral cavities, may induce and perpetuate periodontitis when their concentration rises in the gingival sulcus. Antibacterial effect against various Gram-negative and Gram-positive bacteria, including pathogenic and drug-resistant ones, has been shown for several distinct transient metal and metal oxide NPs. Therefore, NPs may be used in biomedicine to treat periodontal problems and in nanotechnology to inhibit the development of microorganisms. Instead of using harmful chemicals or energy-intensive machinery, biosynthesis of metal and metal oxide nanoparticles (NPs) has been suggested. To produce metal and metal oxide NPs, the ideal technique is "Green" synthesis because of its low toxicity and safety for human health and the environment. Gold NPs (AuNPs) appear to be less toxic to mammalian cells than other nanometals because their antibacterial activity is not dependent on reactive oxygen species (ROS). AgNPs also possess chemical stability, catalytic activity, and superior electrical and thermal conductivity, to name a few of their other advantageous characteristics. It was observed that zinc oxide (ZnO) NPs and copper (Cu) NPs exhibited discernible inhibitory effects against gram-positive and gram-negative bacterial strains, respectively. ZnO NPs demonstrated bactericidal activity against the microorganisms responsible for periodontitis. Medications containing magnetic NPs are highly effective against multidrug-resistant bacterial and fungal infections. The titanium dioxide (TiO2) NPs are implicated in elevating salivary peroxidase activity in individuals diagnosed with chronic periodontitis. Furthermore, specific metallic NPs have the potential to enhance the antimicrobial efficacy of periodontitis treatments when combined. Therefore, these NPs, as well as their oxide NPs, are only some of the metals and metal oxides that have been synthesized in environmentally friendly ways and shown to have therapeutic benefits against periodontitis.

A state-of-the-art review on the MicroRNAs roles in hematopoietic stem cell aging and longevity.

Aging is a biological process determined through time-related cellular and functional impairments, leading to a decreased standard of living for the organism. Recently, there has been an unprecedented advance in the aging investigation, especially the detection that the rate of senescence is at least somewhat regulated via evolutionarily preserved genetic pathways and biological processes. Hematopoietic stem cells (HSCs) maintain blood generation over the whole lifetime of an organism. The senescence process influences many of the natural features of HSC, leading to a decline in their capabilities, independently of their microenvironment. New studies show that HSCs are sensitive to age-dependent stress and gradually lose their self-renewal and regeneration potential with senescence. MicroRNAs (miRNAs) are short, non-coding RNAs that post-transcriptionally inhibit translation or stimulate target mRNA cleavage of target transcripts via the sequence-particular connection. MiRNAs control various biological pathways and processes, such as senescence. Several miRNAs are differentially expressed in senescence, producing concern about their use as moderators of the senescence process. MiRNAs play an important role in the control of HSCs and can also modulate processes associated with tissue senescence in specific cell types. In this review, we display the contribution of age-dependent alterations, including DNA damage, epigenetic landscape, metabolism, and extrinsic factors, which affect HSCs function during aging. In addition, we investigate the particular miRNAs regulating HSCs senescence and age-associated diseases. Video Abstract.

Distribution and amount of stresses caused by insertion or removal of orthodontic miniscrews into the maxillary bone: A finite element analysis.

OBJECTIVE: Initial stability of miniscrews is an important factor in their success as orthodontic anchorages. One of the factors affecting this stability is the stresses exerted to the bone by the screw. Since the distribution and extent of stresses and strains produced during insertion or removal of miniscrews had not been measured before, this study used finite element analysis (FEA) to measure these parameters in tapered versus cylindrical screws with or without pilot sockets. MATERIALS AND METHODS: An FEA model of maxilla, pilot hole, and tapered/cylindrical miniscrews were created from 875 CT scan data. The bone cortex was considered 2mm thick. The cancellous bone was reconstructed below the cortical bone. Miniscrews were modelled on the basis of commercial titanium tapered and cylindrical miniscrews (1.6mm wide, 8mm long). The diameter and length of the guiding hole were considered to be 1.1 and 1.5mm, respectively. The miniscrews were inserted (and removed) between the maxillary second premolar and first molar. Stress/strain produced in the bones or screws were measured. RESULTS: During screw insertion, in all setups, the highest stress existed within both the bone and screw, when the screw was in the cortical bone; after insertion into the cancellous bone, the stress suddenly dropped. In cylindrical screws, the highest amount of stress was distributed around the neck which was used for screw driving. In tapered screws, the stress was mostly distributed around the front one-third of the screw. During screw removal, the results of four setups were rather similar with stresses concentrated around screw necks, in the depth of the screw hole, and around the bone surface. The greatest bone stress during insertion was caused by the pilot-less tapered screw (10.18MPa) and the lowest stress was exerted by a pilot-less cylindrical screw (0.74MPa). CONCLUSION: Most of the stress and strain is tolerated by the cortical bone and not the cancellous one. Using cylindrical miniscrews might be more bone-friendly. However, all cases had stresses below tolerable thresholds, and hence are safe.

Reliability of SEMG measurements for trunk muscles during lifting variable loads in healthy subjects.

OBJECTIVE: The reliability of surface electromyography (SEMG) derived parameters is of high importance, but there is distinct lack of studies concerning the reliability during dynamic contractions. Particularly, the similarity index reliability has not been investigated during functional task yet. The purpose of this study was to evaluate the reliability of trunk muscle activities measured by means of surface electromyography during lifting tasks over repeated trials within a day. METHODS: Seven volunteers were assessed twice a day. SEMG signals were recorded bilaterally from seven trunk muscles. Intra class correlation coefficients (ICC) and standard error of similarity index, magnitude and timing were calculated. RESULTS: Investigation of ICC and correlation between trials showed that similarity index (ICC 0.73-0.97, correlation 0.6-0.95) is more reliable and repeatable than muscles magnitude (ICC 0.41-0.69, correlation 0.54-0.65) and it's timing (ICC 0.41-0.69, correlation 0.31-0.74) while timing had the least reliability. CONCLUSION: Similarity index can be mentioned as an appropriate motor control index and evaluation of muscle recruitment pattern should be done by considering similarity index and magnitude.