Asunto(s)
Carcinomatosis Meníngea , Cefalea/etiología , Humanos , Náusea/etiología , Trastornos de la VisiónAsunto(s)
Infecciones Fúngicas del Ojo/etiología , Enfermedades del Aparato Lagrimal/etiología , Rinitis Alérgica/complicaciones , Sinusitis/complicaciones , Adulto , Enfermedad Crónica , Infecciones Fúngicas del Ojo/diagnóstico , Hongos/aislamiento & purificación , Humanos , Enfermedades del Aparato Lagrimal/diagnóstico , Masculino , Rinitis Alérgica/diagnóstico , Rinitis Alérgica/microbiología , Sinusitis/diagnóstico , Sinusitis/microbiología , Tomografía Computarizada por Rayos XAsunto(s)
Oftalmopatías/etiología , Hemiatrofia Facial/complicaciones , Atrofia , Edema Corneal/etiología , Enoftalmia/etiología , Humanos , Iris/diagnóstico por imagen , Iris/patología , Masculino , Persona de Mediana Edad , Hipotensión Ocular/etiología , Fotofobia/etiología , Uveítis/etiología , Trastornos de la Visión/etiologíaRESUMEN
BACKGROUND: In bluegill sunfish, the melanin-containing pigment granules of the retinal pigment epithelium undergo cyclic movements in response both to ambient lighting and circadian cues. Pigment granules aggregate into the cell body at night (in the dark), and disperse into apical processes during the day (in the light). Regulation of pigment granule aggregation in a number of fishes depends on modulating the intracellular levels of cyclic adenosine monophosphate. RESULTS: Here we show isolated RPE takes up cyclic adenosine monophosphate (cAMP) in a saturable manner, exogenously applied cAMP induces pigment granule aggregation in retinal pigment epithelium isolated from bluegill, and aggregation induced in this manner is inhibited by treatment with probenecid, an organic anion transport inhibitor. CONCLUSION: Our results raise the possibility that cAMP functions as a messenger secreted from the neural retina to signal darkness to the RPE, which takes it up. It further suggests that organic anion transport systems are the route by which cAMP crosses RPE cell membranes since probenecid inhibits extracellular cAMP from causing pigment granule aggregation.