Histological response of inflamed pulp to hydraulic calcium silicate cements in direct pulp capping: Systematic review of pulpitis models.

This systematic review aimed to compare the histological response of inflamed pulpodentinal complex to the hydraulic calcium silicate cements in experimental animal models of pulpitis. Articles that evaluated the histological response of inflamed pulp to mineral trioxide aggregate (MTA) in comparison with other restorative materials were selected and analysed in detail. The risk of bias assessment was conducted using SYRCLE's RoB tool. The GRADEpro tool was used to determine the overall quality of evidence. Out of the 2947 retrieved articles from databases, five articles fulfilled the inclusion criteria. MTA induced significantly more hard tissue formation compared to calcium hydroxide. The use of pulp-capping material containing fluocinolone acetonide and ASP/PLGA-ASP/ACP/PLLA-PLGA composite membrane was comparable. This systematic review could not demonstrate enhanced efficiency of capping materials compared to MTA. Future well-conducted animal studies are warranted for demonstrating the hard tissue formation abilities of pulp-capping materials with convenient inflammatory conditions.

Shaping outcomes of Reciproc Blue and Rotate in roots canals with band-shaped isthmuses: micro-CT study.

OBJECTIVE: This study assessed the outcome of shaping the mesial roots of mandibular first molars containing band-shaped isthmuses with an isthmus floor located at the apical third with Reciproc Blue and Rotate instruments in terms of canal transportation, canal aberrations and unprepared apical canals. MATERIALS AND METHODS: Pair-matched mesial roots of mandibular first molars according to their isthmus location (distance from apical foramina) and size were constructed based on quantitative measurements (length, major and minor diameter at isthmus roof and floor) on preoperative micro-CT scanning (n = 10). The pair-matched specimens were prepared either using Reciproc Blue R25 (25.08v) or Rotate (15.04, 20.05, 25.06) instruments and then subjected to a second micro-CT scanning. Micro-CT scans were analysed to evaluate the canal transportation at the apical foramen and isthmus floor levels and the frequency of procedural errors (separated instruments, artificial canal preparation, cross shaping and unprepared apical canals). Canal transportation values were compared using Mann-Whitney U, and the distribution of procedural errors was compared with chi-square tests with a 5% significance threshold. RESULTS: Greater transportation values were detected in the apical foramen level compared to the isthmus floor in both groups. No significant difference was detected between Rotate and Reciproc Blue groups in terms of absolute transportation values and the incidence of procedural errors (p > .05). CONCLUSION: Preparation of mesial root canals containing a band-shaped isthmus at the apical portion with Reciproc Blue and Rotate instruments caused transportation of the original canal position at the isthmus floor and the apical foramen levels and resulted in procedural errors. CLINICAL RELEVANCE: This ex vivo micro-CT study using controlled pair-matched specimens showed that presence of a band-shaped isthmus complicates canal preparation procedure irrespective of the preparation systems.

Age-related changes in the morphology of the root canal system of mandibular first molars: a micro-CT study.

OBJECTIVE: This study aimed to evaluate changes on root canal morphology in patients with different ages using micro-CT technology. MATERIALS AND METHODS: Mandibular first molars (n = 150) were scanned at a pixel size of 13.68 µm, categorized into 3 groups according to patient's age and analyzed regarding configuration, orifices, apical foramina, root length, canal volume, and surface area. Morphological 2D and 3D parameters were evaluated in distal roots with Type I configuration (n = 109) as well as the morphology of isthmuses Types I and III in 68 mesial roots. One-way ANOVA post hoc Tukey and Kruskal-Wallis tests were used for statistical analyses (α = 5%). RESULTS: A great variation in the canal configuration was found. No difference was observed in roots' length (p > 0.05). Canal volume reduced with age (p < 0.05), while surface area increased (p < 0.05) in patients ≤ 30 years. There was no difference in canal/root length, area, and from foramen to the apex in distal roots with Type I configuration (p > 0.05), but 2D and 3D parameters significantly decreased with age (p < 0.05). Overall, the diameter of the isthmuses' roof reduced with age (p < 0.05). In patients ≥ 31 years with Type III isthmus the distance from the isthmus floor to the foramen of the mesiolingual canal also decreased (p < 0.05). CONCLUSION: Overall, the internal morphology of the mesial roots of mandibular first molars was more affected by aging than distal canals. The most relevant tested parameter that significantly reduced in both roots was the volume of the root canal systems. CLINICAL RELEVANCE: A detailed evaluation of fine anatomical aspects of the root canal system of mandibular first molars of patients with different ages showed that the internal morphology of mesial roots is more affected by aging than distal canals.

Dentine thickness in maxillary fused molars depends on the fusion type: An ex vivo micro-computed tomography study.

AIM: The present study aimed to determine and compare the dentine thickness around the root canals of maxillary molars with fusion using micro-computed tomography (micro-CT). METHODOLOGY: A total of 120 fused maxillary molars having more than a single canal with distinct canal orifices near the cementoenamel junction were selected from a pool of extracted maxillary molars, which were scanned on a micro-CT device (SkyScan 1172, Bruker-microCT). The minimum dentine thickness around the root canals in furcal direction was measured using CTAn software (v.1.18.8 Bruker-microCT) at each millimetre. The specimens were grouped according to their fusion type, and dentine thickness around the canals was compared. The data were statistically analysed using anova and post hoc Tukey-Kramer tests following an assessment of the normality of their distribution with the Kolmogorov-Smirnov test (p < .05). RESULTS: Dentine thickness showed a statistically significant decrease at 2 mm apical to the orifice level irrespective of fusion type. The minimum dentine thickness values were detected around the second mesiobuccal canal as 0.30 mm at 6 mm below orifice. Dentine thickness around the mesiobuccal canal was significantly greater in fusion type 1 than those of types 2 and 3 (p < .05), while those of distobuccal and palatal canals were significantly thinner in type 3 fusion compared with type 1 or 6 (pDentine thickness showed a statistically significant decrease at 2 mm apical to the orifice level  irrespective of fusion type. The minimum dentine thickness values were detected around the second mesiobuccal canal as 0.30 mm at 6 mm below orifice. Dentine thickness around the mesiobuccal canal was significantly greater in fusion type 1 than those of types 2 and 3 ( CONCLUSION: Minimum dentine thickness values at the danger zone of distobuccal and palatal canals change according to the fusion type in fused maxillary second molars.

Comparison of mechanical resistance and standardisation between original brand and replica-like endodontic systems.

This study compared the original (ProTaper Next and Reciproc) endodontic systems with their replica-like brands (X File and Only One File) in terms of standardisation, design, phase-transformation behaviour, composition and mechanical behaviour. X File showed greater taper values than ProTaper Next, while Only One File had the greatest tip diameter. Both replica-like files had an active tip and greater dimensions than their reports. There were also significant differences between the original and replica-like systems in terms of their phase-transformation behaviour and the precision of the measurement lines. Only One File showed significantly lower cyclic fatigue and torsional resistance than Reciproc (p < 0.05). There were no significant differences in the cyclic fatigue, torsional resistance and composition of NiTi between X File and ProTaper Next (p > 0.05). Although replica systems show mechanical properties that can be acceptable, they are not consistent in terms of standardisation and design.

Efficacy of Endoscope and Periapical Radiography to Detect Residual Root Canal Filling Using Micro-CT as Reference.

INTRODUCTION: To evaluate the efficacy of examinations by endoscope of root canals to determine residual root canal filling materials by comparison with microcomputed tomographic (micro-CT) images as reference. METHODS: Thirty maxillary second premolars with oval canals received root canal treatment. Then D-Race retreatment files were used to remove the root canal fillings supplemented by XP-endo Finisher. Samples were scanned to reconstruct micro-CT reference images. Images were obtained of all samples from the mesiodistal and buccolingual directions by digital radiography. Then the root canals of specimens mounted in manikins were assessed by endoscope. The radiographic and endoscopic results were scored following comparison with the micro-CT images, which served as a reference, by 2 blinded observers. The interexaminer reliability of the assessments was evaluated by kappa test and the accuracy of endoscopic visualization according to the presence of residual root canal filling materials was compared using Kruskal-Wallis H test and Spearman's rank order correlation with the level of significance set at 5%. RESULTS: The efficiency of the periapical radiography and endoscopy to determine residual root canal filling material was similar in every third of the root canal (P > .05). CONCLUSIONS: The endoscope detected residual root canal filling material as efficiently as periapical radiography.

Comparison of postoperative pain after the use of different nickel titanium instrumentation systems: a randomized clinical trial.

Purpose: Postoperative pain is a common complication in endodontics contributed by multiple etiological factors, which consist canal preparation instruments and kinematics. The aim of this randomized clinical trial compare the postoperative pain in terms of intensity and incidence after the use of different nickel titanium (NiTi) file systems. Patients and methods: In this randomized clinical trial (NCT03791762), a total of 150 patients were root canal treated by 2 experienced endodontists according to a standardised protocol. The subjects were randomly assigned to 1 of the 3 groups according to preparation instrument used: ProTaper Next (Dentsply Sirona, Ballaigues, Switzerland), Reciproc Blue (VDW, Munich, Germany) and WaveOne Gold (Dentsply Sirona). Following preparation the teeth underwent standardized root canal treatment procedures in a single visit. The patients were contacted to gather information about the incidence of pain and intensity at 6th, 12th, 18th, 24th, 48th, and 72nd hours postoperatively. The data were analysed using chi-square, one-way analysis of variance and post hoc Tukey tests and logistic regression analysis with 5% significance threshold. Results: No significant difference was found among preparation groups in relation to the intensity of postoperative pain. The incidence of postoperative pain was significantly linked with the preoperative pain presence with odds ratio values ranging between 2.06 and 4.08 irrespective of the preparation technique (P<0.05). Conclusion: The effects of reciprocating and the continuous rotary systems on the intensity and incidence of postoperative pain were found to be similar.

Are aerosol control devices effective in preventing the spread of dental aerosol?

Background: In dental clinics, aerosols produced from dental instruments have become a matter of concern following breakout of coronavirus disease 19 (COVID-19) evolving into a pandemic. This study compared aerosol reduction systems and in terms of their ability to reduce Enterococcus faecalis (E. faecalis) contaminated aerosol in a simulated dental office set-up. Methods: Closed clinic model with manikin and mandibular molar typodont was simulated. For 10 min, the air and water dispersed by the rotating bur mounted on an aerator was contaminated by pouring the suspension containing 1-3 × 108 CFU/mL E. faecalis directly on the bur. During and after the procedures, the air within the cabin was also sampled. CFU count was recorded and scored. The mean CFU scores obtained from agar plate count and air sampling device was compared using Kruskal-Wallis H test among groups with 5% significance threshold. Results: The use of WS Aerosol Defender device led to greater CFU scores on the agars levelled to patient's chest compared to other directions (p = 0.001). Combined use of VacStation and WS Aerosol Defender resulted in significantly decreased CFU score in the air samples compared to experimental and positive control groups (p = 0 < 0.05). Conclusions: Although the devices prevented the spread of aerosol around the patient to some extent, they could not completely eliminate the contaminated aerosol load in the cabin environment.

Evaluation of the band-shaped isthmuses in the mesiobuccal root canal system using micro-computed tomography.

OBJECTIVES: This micro-computed tomography (micro-CT) study analyzed band-shaped isthmuses having their floor at the apical third of the mesiobuccal roots of maxillary molars in terms of 2D and 3D morphological parameters. MATERIALS AND METHODS: A total of 199 maxillary first molars were scanned with micro-CT. Twenty-seven specimens, confirmed to have a band-shaped isthmus, were further examined in terms of the isthmus length, volume, structure model index (SMI), and surface area. The distance of apical foramina of mesial canals from the isthmus floor and major-minor diameters, roundness, perimeter, and area of the isthmus roof and floor were compared. Statistical analyses were performed with descriptive statistics and t-test with a 5% significance threshold. RESULTS: The band-shaped isthmus frequency was 13.5% and its length reached up to 5.5 mm. The distances of the apical foramina of mesiobuccal canals (MB1 and MB2) from the isthmus floor were similar (p > .05). Isthmus roof and floor showed significant differences in terms of their major diameter and area (p < .05), whereas their minor diameter, roundness, and perimeter values were similar (p > .05). CONCLUSION: The band-shaped isthmuses are not rare and can reach through the half of the root length with oval cross-sections in the floor and roof anatomy. The distance between the isthmus floor and apical foramina did not differ between MB1 and MB2. CLINICAL RELEVANCE: The band-shaped isthmus morphology contributes to the complexity of the apical anatomy of maxillary first molar teeth with various lengths and diameters.

Micro-CT assessment of radicular pulp calcifications in extracted maxillary first molar teeth.

OBJECTIVES: The present study aimed to evaluate the frequency, position, and length of radicular pulp calcifications in three-rooted maxillary first molars using micro-computed tomography. MATERIALS AND METHODS: Two hundred three-rooted maxillary first molars were scanned at a pixel size of 10 µm and reconstructed using similar parameters. Three-dimensional models of the mesiobuccal (MB1, MB2, MB3), distobuccal (DB), and palatal (P) canals were qualitatively evaluated for discontinuity in the canal path. Transaxial and sagittal cross-sectional images of the roots were further screened, and the complete obliteration of the canal lumen by pulp calcification was identified when the canal had similar radiodensity to the surrounding dentine. Then, the number, position, and length of the calcifications were recorded for each root. Intra-observer agreement was performed using Cohen's kappa test, while one-way ANOVA and Duncan tests compared the lengths of calcifications among the canals, with α = 5%. RESULTS: Intra-observer agreement was perfect for identification of pulp calcifications (ĸ = 1.0; p = 0.000). MB2 and MB3 canals accounted for the highest percentage frequency of calcifications distributed throughout the canal path. In the MB1, DB, and P canals, they occurred mainly at the apical third. Lengths of calcifications were greater in the MB3 (0.89 ± 0.81 mm) and MB2 (0.82 ± 0.93 mm) canals than in the MB1 (0.39 ± 0.32 mm), DB (0.34 ± 0.22 mm), and P (0.28 ± 0.22 mm) canals (p < 0.05). CONCLUSION: MB2 and MB3 canals accounted for the highest frequency and length of radicular pulp calcifications. In the MB1, DB, and P canals, calcifications occurred mostly at the apical third, while in the MB2 and MB3, most of calcifications were observed at the orifice level and along the canal path. CLINICAL RELEVANCE: In maxillary first molars, radicular pulp calcifications are mainly located at the apical third of the MB1, DB, and P canals, while in the MB2 and MB3 canals, most of them are at the orifice level or along the canal path.

Metallurgical characterisation and torsional resistance of blue thermomechanically treated nickel titanium instruments after simulated ex vivo retreatment procedure.

To compare used and unused Reciproc Blue and Reciproc after retreatment, the instruments were used in the mesiobuccal canals of maxillary molars with curvature. The torsional resistances were tested and then unused instruments were subjected to XRD, DSC and microhardness tests. Student's t, ANOVA and Tukey's tests were performed (α-level 0.05). The torsional strength of used Reciproc Blue was not significantly different than unused ones (P > .05), while torsional strength of Reciproc decreased significantly (P < .05). DSC and XRD indicated that Reciproc Blue exhibited prominent transformation peaks corresponding austenite to R-phase. Microhardness of Reciproc was significantly higher (P < .05). A second reuse of Reciproc Blue for retreatment could be considered safe in terms of torsional strength. Blue treatment also decreased surface microhardness of the instrument and changed phase composition by increasing the R-phase.

Static Cyclic Fatigue Resistance in Abrupt Curvature, Surface Topography, and Torsional Strength of R-Pilot and ProGlider Glide Path Instruments.

INTRODUCTION: This study aimed to compare ProGlider (Dentsply Sirona, Ballaigues, Switzerland) and R-Pilot (VDW, Munich, Germany) instruments in terms of their cyclic fatigue resistance using an artificial stainless steel canal showing an abrupt apical curvature, torsional resistance according to the ISO specification, and topographic changes on the instrument surface after glide path management in mesial canals of mandibular first molars with the abrupt curvature selected based on their micro-computed tomographic examination. METHODS: Eighty instruments were used: 40 ProGlider (size 0.16, .02v taper) and 40 R-Pilot (size 0.125, .04 taper) instruments. The cyclic fatigue resistance was tested in a static test model using an artificial canal with an abrupt apical curvature (angle of curvature of 90° and radius of curvature of 2 mm). The torsional resistance test was performed according to ISO 3630-1 specifications. To determine surface topography of the unused and used instruments, mesial root canals of mandibular molars with an abrupt apical curvature were selected to prepare a glide path with either the ProGlider or R-Pilot instrument. An optical profilometer and scanning electron microscopy were used to determine the surface properties. Normally distributed torsional and cyclic resistance data were analyzed using the Student t test, whereas quantitative data obtained by the optical profilometer were analyzed with the Kruskal-Wallis H test with a 5% significance threshold. RESULTS: The R-Pilot showed significantly higher cyclic fatigue and torsional resistance than the ProGlider (P < .05). Angular deflection values were similar between instruments (P < .05). Measurements made from the blade area showed that the surface roughness values of the ProGlider were larger. Cutting blade measurements showed that unused instruments had significantly greater roughness values than used ones (P < .05). Although there was a 14% increase between the blade edge radii of the used and unused R-Pilot instruments, this difference was determined as 61% in ProGlider instruments. CONCLUSIONS: The R-Pilot exhibited greater cyclic fatigue strength than the ProGlider when tested in an artificial canal with an inner diameter of 1.0 mm and an abrupt apical curvature. Torsional resistance of the R-Pilot was higher than the ProGlider, but the angular deflection values were similar. Glide path preparation in a mesial root canal with an abrupt apical curvature did not increase the surface roughness of both instruments but resulted in a greater blade edge radius.

Diagnostic accuracy of endoscopy for the detection of isthmuses of mandibular molar teeth using micro-CT as reference.

PURPOSE: The aim of this study was to evaluate the efficacy of endoscopic visualisation to detect the presence and type of isthmuses within the mesial root canals of mandibular first molar teeth compared with micro-computed tomography (micro- CT) images as reference. MATERIALS AND METHODS: Thirty-two mesial roots of mandibular first molars presenting isthmuses were selected based on micro-CT scans. In all, 12 type I and 20 band-shaped isthmuses were collected. The specimens were mounted in the posterior socket of dental phantom manikin for endoscopic visualisation. The ability of endoscopes to visualize the presence of isthmuses and distinguish the type of isthmuses was compared. Micro-CT images of the specimens were used as references. Data were analyzed using Fisher's exact tests. RESULTS: Sensitivity of endoscope to detect isthmuses were also calculated for each isthmus type. In 37.5% of the samples, isthmus presence was correctly diagnosed via orthograde endoscopic visualization. Type I istmuses were significantly more detected than band-shaped isthmuses (P<0.05). Endoscope showed higher sensitivity to detect type I isthmus than band-shaped isthmus. CONCLUSION: The endodontic endoscope could detect type I isthmuses more accurately than band- shaped isthmuses.

Comparison of the Torsional Resistance of 4 Different Glide Path Instruments.

INTRODUCTION: The present study aimed to compare the torsional strength of the initial files of the Mtwo (VDW, Munich, Germany) and novel Rotate systems (VDW, Munich, Germany) with the ProGlider (Dentsply Sirona, Ballaigues, Switzerland) and R-Pilot (VDW) glide path instruments. METHODS: The Mtwo (10/.04), ProGlider (16/.02), R-Pilot (12.5/.04), and Rotate (15/.04) glide path files were compared regarding their torsional strength, which was tested using a specially designed test device (N = 20). The data obtained were statistically analyzed at 5% significance level using 1-way analysis of variance and the post hoc Tukey test. The fragment surfaces and separated instruments were examined with ×50, ×100, and ×1000 magnification under a scanning electron microscope. RESULTS: The R-Pilot group showed the highest torsional strength value among all groups (P < .05), whereas there was no significant difference between the torsional strength values of the Mtwo and ProGlider groups (P > .05). The Rotate group had the lowest torsional strength among all groups (P < .05). The Mtwo group showed the lowest angle of rotation among all groups (P < .05). CONCLUSIONS: Although the R-Pilot glide path file exhibited the highest torsional strength in all groups, Rotate showed the highest angle of rotation. Differences in torsional resistance of the instruments may be associated with their manufacturing methods and design features.

Mesiobuccal and Palatal Interorifice Distance May Predict the Presence of the Second Mesiobuccal Canal in Maxillary Second Molars with Fused Roots.

INTRODUCTION: The aim of this study was to evaluate if the configuration of canal orifices can predict the presence of a second mesiobuccal canal (MB2) in maxillary second molars with fused roots. METHODS: Maxillary second molars with fused roots (N = 150) were scanned in a micro-computed tomographic device (pixel size = 9 µm) and evaluated regarding the root fusion type and the incidence of the MB2 canal. The centers of the canal orifices were connected, and the angles formed by the intersection of these lines as well as their distances were measured and statistically compared (1-way analysis of variance). Specimens were then assigned to 2 groups according to the presence (n = 65) or absence (n = 50) of the MB2 canal and compared regarding the angles and interorifice distances using the Welch t test. A binary logistic regression model estimated the association between the interorifice distance, the fusion type, and the presence of the MB2 canal, whereas receiver operating characteristic curve analysis was performed to evaluate the diagnostic abilities of significant variables. The significance level was set at 5%. RESULTS: The most common fusion types were 1 and 6, and MB2 canal incidence was 47.3%. No statistical difference was observed in the angles or interorifice distances among teeth with different fusion types (P > .05). Distances from the mesiobuccal (MB) to the distobuccal and palatal (P) canal orifices as well as the MB and P angles were significantly higher in the presence of an MB2 canal (P < .05). A binary logistic regression model revealed that MB-distobuccal and MB-P distances were significant in the presence of an MB2 canal (P < .05). Receiver operating characteristic analysis indicated that MB-P distance had acceptable diagnostic accuracy for the prediction of MB2 canal presence. Sensitivity (specificity) calculated from the MB-P distances of 4.0, 4.5, and 5.0 mm were 89.2% (36.5%), 76.9% (58.4%), and 44.4% (71.4%), respectively. CONCLUSIONS: MB-P interorifice distance is a strong predictor of the presence of the MB2 canal in maxillary second molars with fused roots.

Efficacy of glycolic acid for the removal of calcium hydroxide from simulated internal Resorption cavities.

OBJECTIVES: This study evaluated the efficacy of 5% and 10% glycolic acid solutions for the removal of calcium hydroxide medicament from artificial internal resorption cavities. MATERIALS AND METHODS: A total of 170 human maxillary premolars were selected and artificial internal resorption cavities were prepared using round burs and 37% orthophosphoric acid in the apical third of the root halves. Entire canal and resorption cavity were filled with calcium hydroxide paste. Then, the specimens were assigned to 2 control groups or ten experimental groups according to the irrigating solutions used for medicament removal with and without activation with ultrasonic energy as following: 5% glycolic acid, 10% glycolic acid, 17% EDTA, and 10% citric acid and distilled water. The resorption cavities were examined under different magnifications using stereomicroscopy and scanning electron microscopy (SEM). The calcium hydroxide remnants in the resorption cavity were scored by 2 evaluators using a 4-scoring scale. The data were analyzed with Kruskal-Wallis H tests with 5% significance threshold. RESULTS: There was no significant difference regarding the distribution of removal scores among non-activated solutions (P > 0.05). In activated groups, 10% glycolic acid irrigation removed significantly more amount of calcium hydroxide than EDTA and distilled water (P < 0.05). No significant difference was found between the efficacy of 10% glycolic and citric acid (P > 0.05). Citric acid, 5% glycolic acid, and EDTA showed similar removal scores (P > 0.05). Passive ultrasonic irrigation significantly improved calcium hydroxide removal scores in 10% glycolic acid, citric acid, and EDTA groups (P < 0.05). The SEM examination revealed that the specimens that were scored 0 are not entirely free of calcium hydroxide remnants. CONCLUSION: Concentration of 10% glycolic acid removed significantly more calcium hydroxide paste from resorption cavities than EDTA when used with passive ultrasonic irrigation. Although passive ultrasonic irrigation favored medicament removal in all irrigating solutions, complete elimination of medicament remnants was unattainable. CLINICAL RELEVANCE: This study showed the improved efficacy of ultrasonically activated 10% glycolic acid in removing the calcium hydroxide medicament from the internal resorption cavity.

Comparison of cyclic fatigue resistance of Rotate instrument with reciprocating and continuous rotary nickel-titanium instruments at body temperature in relation to their transformation temperatures.

OBJECTIVE: The aim of this study was to compare the cyclic fatigue resistance at body temperature and phase transformation behaviors of novel Rotate instrument (25.06) with rotating Mtwo (25.06) and reciprocating Reciproc Blue (25.08) and Reciproc (25.08) instruments. MATERIALS AND METHODS: The Rotate, Reciproc Blue, Reciproc, and Mtwo instruments free of visible deformations were collected and tested in a static cyclic fatigue test method, which has a ceramic block containing an artificial canal with 60° angle of curvature and a 5-mm radius of curvature at 37 °C (n = 16). All instruments were operated until fracture occurred, and both time to fracture (TF) and the lengths of the fractured fragments were recorded. TF data was analyzed with one-way ANOVA followed by post hoc Tukey tests and Weibull analysis, and fractured fragment length data were subjected to one-way ANOVA and post hoc Tukey tests (P < 0.05). Two unused instruments from each brand were also subjected to differential scanning calorimetry (DSC) analysis to determine their phase transformation temperatures. RESULTS: The Reciproc Blue instruments showed significantly higher TF values and reliability than the other groups (P < 0.05). Rotate instruments exhibited greater cyclic fatigue resistance than the Reciproc and Mtwo instruments (P < 0.05). No significant difference was detected among the fractured fragment lengths (P > 0.05). The lowest austenite finish temperature was exhibited by the Mtwo, which presented a single transformation peak, followed by the Rotate, Reciproc Blue, and Reciproc instruments which all presented two peaks during transformation. CONCLUSIONS: Cyclic fatigue resistance of instruments manufactured from thermally treated Blue wire instruments was superior to those of the Mtwo and Reciproc, whereas reciprocating the Blue wire showed the highest resistance. CLINICAL RELEVANCE: The present study compared the cyclic fatigue resistance of the novel Rotate instrument with similar instruments manufactured from conventional nickel-titanium, m-wire, and Blue wire at body temperature and reported that Blue-treated instruments exhibited superior cyclic fatigue resistance.

Visualization and characterization of Enterococcus faecalis biofilm structure in bovine dentin using 2D and 3D microscopic techniques.

Bacterial biofilms are related to various dental and periodontal infectious diseases, and the characterization of this biological structure with micro-computed tomography (micro-CT) may offer valuable information for clinical and research applications. In this study, we aimed to develop a model to visualize three-dimensionally the biofilm structure on dentin using micro-CT. Dentin blocks were prepared and incubated in tryptic soy broth with Enterococcus faecalis (ATCC 29212). The control group did not receive any staining procedure, while groups 1 and 2 were stained with 100% and 50% barium sulfate, respectively. Transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) were used to detect biofilm formation, barium sulfate penetration, and microbial cell density in the biofilm. Micro-computed tomography (micro-CT) (SkyScan 1172, Bruker Co., Belgium) was used to visualize biofilm formation on the dentin blocks. Biofilm thicknesses were measured from 10 different locations on the specimen surfaces, using CTAn v.1.14.4 software. Obtained data were statistically analyzed using Kruskal-Wallis and Dunn's tests. TEM photomicrographs showed that barium sulfate could penetrate the biofilm structure. CLSM analysis showed that viable and total cell densities were similar between the control and barium sulfate-treated groups (P > 0.05), indicating barium sulfate had no significant influence on cell density. In barium sulfate-treated blocks, biofilm could be discriminated from the dentin, and its thickness could be measured with micro-CT. This study showed that bacterial biofilm on dentin could be characterized by micro-CT after barium sulfate staining without causing any significant side effect on viable and total cell densities.

Apically extruded debris produced during glide path preparation using R-Pilot, WaveOne Gold Glider and ProGlider in curved root canals.

The present study aimed to compare the apically extruded debris produced during glide path preparation using R-Pilot (RP), WaveOne Gold Glider (WGG) and ProGlider (PG) with the subsequent preparation using Reciproc Blue in curved root canals. Mesial roots of mandibular molars with angle of curvature between 25° and 35° were selected (n = 20). The Myers and Montgomery method was used to collect and assess apically extruded debris. The glide path was prepared either manually with K-file, ProGlider, R-Pilot or WaveOne Gold Glider. Manual preparation group produced the highest amount of apically extruded debris (P < 0.05), while all groups caused debris extrusion. There were no significant differences among the extruded debris caused by RP, PG and WGG groups (P > 0.05). Apically extruded debris produced by engine-driven reciprocational or continuous rotational single file glide path instruments was similar, whereas manual glide path preparation was associated with the greatest amount of extruded debris.

Micro-computed tomographic analysis of the mesial root of mandibular first molars with bifid apex.

OBJECTIVES: The aim of the present study was to evaluate the influence of bifid and non-bifid configurations of the mesial root of mandibular first molars in the canal anatomy using micro-computed tomography (micro-CT). DESIGN: Thirty mesial roots with bifid apex were pair-matched with 30 non-bifid roots by means of micro-CT. Bifid and non-bifid mesial roots were compared regarding morphological aspects at the apical third, dentin thickness, and the presence of isthmus and middle mesial canal (MMC). Data were analyzed using Chi-square test and Student's t-test with a significant level of 5 %. RESULTS: Main foramen of mesiobuccal (MB) and mesiolingual (ML) canals were positioned within 2.5 mm from the anatomic apex, and origin and exit of accessory canals were observed mostly between 1.0 and 2.0 mm from the apex in the groups. Despite no statistical difference was observed between bifid and non-bifid roots regarding the number of canal orifices (p > 0.05), the frequency of accessory canals was significantly higher in bifid roots (p < 0.05). Regarding the dentin thickness, a statistically significant difference was observed between bifid and non-bifid roots at the furcation level (p < 0.05). Only 3 mesial roots with bifid apex (10 %) had isthmuses, mostly at the coronal third, while a high incidence of MMC was observed in this group (n = 10, 33.3 %). CONCLUSIONS: Presence of bifid apex in the mesial root of mandibular first molars might be a predictive factor for a complex canal anatomy at the apical third with an increasing number of accessory canals.