[Metabolism of organic nitrogen compounds by carboxydobacteria]. / Metabolizm organicheskikh azotnykh soedinenii u karboksidobakterii.

The capability to hydrolyze and assimilate creatinine, uric and hippuric acids as sources of nitrogen, carbon and energy was studied in Comamonas compransoris Z-1155, Seliberia carboxydohydrogena Z-1062 and Pseudomonas gazotropha Z-1156. The organisms effectively hydrolyzed these compounds and assimilated their nitrogen under the conditions of autotrophic growth. The carboxydobacteria were also capable of growing in media containing creatinine, uric and hippuric acids and assimilated these compounds as carbon sources. In the absence of extraneous energy sources, none of the organisms grew in media with organic nitrogen compounds though the latter were hydrolyzed.

[Autotrophic cultivation of carboxide bacteria in continuous cultures]. / Avtotrofnoe kul'tivirovanie karboksidobakterii v protochnykh usloviiakh.

The carboxydobacterium Seliberia carboxydohydrogena Z-1062 was grown under the conditions of continuous cultivation in the atmosphere of hydrogen, oxygen, carbon dioxide, and carbon monoxide. The concentration of the bacterial cells in the chemostat depended on the specific growth rate at the inhibition with CO. The biochemical composition of the carboxydobacterial biomass, the rate of utilization of the gaseous mixture by the culture, and the economic coefficients were studied when the flow rate of the medium changed from 0.2 to 0.05 hr-1. A stable turbidostatic culture of this carboxydobacterial strain has been obtained, and the effect of CO concentration on the specific growth rate of the bacterium has been assayed.

[Determination of the limits of the physiological action of mineral elements in hydrogen bacteria]. / Opredelenie granits fiziologicheskogo deistviia mineral'nykh élementov u vodorodnykh bakterii.

The growth of the hydrogen bacterium Hydrogenomonas eutropha Z-1 was studied in the conditions of continuous cultivation at different content of mineral elements in the medium. The physiological action of phosphorus, sulfur, potassium and magnesium in the bacterium was found to be displayed within a wide range of concentrations (mg/ml): P, 18-1400; S, 5.5-350; K, 4-550; Mg, 4.8-430. If the control of mineral elements was above or below these values, the specific growth rate of the cells decreased and their intracellular chemical composition changed.

[Effects of the nitrogen nutrition conditions on the growth and protein synthesis of carboxydobacteria]. / Vliianie uslovii azotnogo pitaniia na rost i sintez belkov u karboksidobakterii.

The rate of growth of bacterial strains oxidizing carbon monoxide (Pseudomonas gazotropha Z-1156, Comamonas compransoris Z-1155, and Seliberia carboxydohydrogena Z--1062) was studied as a function of the concentration of NH4Cl in the medium. The bacteria could grow on media containing various nitrogen sources (NH4Cl, KNO3, CO(NH2)2). Changes in the amino acid content and biochemical composition of the biomass were studied during growth of the bacteria on these media. The biological value of proteins of the bacteria was estimated.

[Possibility of culturing hydrogen bacteria in the presence of CO]. / O vozmozhnosti kul'tivirovaniia vodorodynkh bakterii v prisutstvii CO.

The growth of Hydrogenomonas eutropha Z-1 was studied in the presence of CO in the gaseous phase. The culture was found to be resistant to the action of carbon monoxide at concentrations of 5 per cent (periodic cultivation) and 7 per cent (continuous cultivation). The hydrogen bacteria can assimilate CO as a source of carbon.

[Continuous cultivation of hydrogen bacteria with multiple use of the medium]. / Nepreryvnoe kul'tivirovanie vodorodnykh bakterii s mnogokratnym ispol'zovaniem sredy

The possibility of continuous cultivation of hydrogen bacteria was studied in the conditions of recirculation of the medium. The hydrogen bacteria accumulated proteins, carbohydrates, lipids, organic and amino acids in the medium during its multiple reutilization, which however had no effect on the specific growth rate and the ability of the bactetia to oxidize molecular hydrogen.