[A study of sera positive and doubtful for antibodies to the human immunodeficiency virus for the presence of HTLV-I-specific antibodies]. / Issledovanie syvorotok, polozhitel'nykh i somnitel'nykh k virus immunodefitsita cheloveka, na nalichie HTLV-I-spetsificheskikh antitel.

The results of examinations for the presence of antibody to HTLV-I of 205 sera which were positive or indefinite (doubtful) with regard to HIV are presented. No evidence indicating simultaneous infection with HIV and HTLV-I viruses in 18 HIV-positive subjects was obtained. Antibodies to gag-proteins p19 and p28 HTLV-I were detected in one serum showing primary reactivity in screening for HIV antibodies. Problems of HIV and HTLV-I occurrence and sensitivity of screening test systems are discussed.

[Increase in the specificity of immunoenzyme analysis by using a monoclonal antibody-based conjugate]. / Povyshenie spetsifichnosti immunofermentnogo analiza za schet ispol'zovaniia kon"iugata na osnove monoklonal'nykh antitel.

Examinations by EIA of laboratory cultures of various orthopoxviruses and specimens from human-monkey pox cases demonstrated the advantages of a monoclonal preparation over the peroxidase polyclonal conjugate prepared from hyperimmune vaccinia antiserum.

[Preparation and comparative evaluation of peroxidase conjugates based on pure antibodies and the IgG Fab fragment]. / Poluchenie i sravnitel'naia otsenka peroksidaznykh kon'iugatov na osnove chistykh antitel i Fab-fragmenta IgG.

The results of the preparation of peroxidase conjugates on the basis of the Fab-fragment of rabbit antivaccinal serum IgG and pure antirabbit IgG are presented. Peroxidase conjugates prepared on the basis of highly purified antibodies have been found (in vaccine virus-infected cell cultures) to ensure greater reliability of the immunoperoxidase method due to the decrease of nonspecific reactions registered by the control test system. This allows recommending peroxidase conjugates prepared on the basis of higher purified antibodies for use in diagnostic tests.

[Use of the immunoperoxidase method for the rapid detection of togaviruses, orthopoxviruses and their antibodies]. / Primenenie immunoperoksidaznogo metoda dlia bystroi indikatsii nekotorykh togavirusov, ortopoksvirusov i antitel k nim.

The successful application of the immunoperoxidase method of (IP) with light microscopy for rapid detection of certain togaviruses and orthopoxviruses and antibodies to them is described. A comparative evaluation of the IP and immunofluorescent (IF) method for these purposes was performed. The results indicate practically similar sensitivity and specificity of both methods. The detection of viral antigen directly depended upon the infective dose and time of examinations (within 4--12 hours postinoculation). In evaluating the IP method, however, the account should be taken of the fact that it does not require expensive equipment and therefore may be used in any practical laboratory or even under field conditions. Rapid detection of both viral antigens and antiviral antibody by this method opens approaches for its wide application in the future.