Influence of the chronic groundwater fluoride consumption on cholinergic enzymes, ACHE and BCHE gene SNPs and pro-inflammatory cytokines: A study with Pakistani population groups.

Fluoride is one of the abundant elements found in the Earth's crust and is a global environmental issue. The present work aimed to find the impact of chronic consumption of fluoride contained groundwater on human subjects. Five hundred and twelve volunteers from different areas of Pakistan were recruited. Cholinergic status, acetylcholinesterase and butyrylcholinesterase gene SNPs and pro-inflammatory cytokines were examined. Association analysis, regression and other standard statistical analyses were performed. Physical examination of the fluoride endemic areas' participants revealed the symptoms of dental and skeletal fluorosis. Cholinergic enzymes (AChE and BChE) were significantly increased among different exposure groups. ACHE gene 3'-UTR variant and BCHE K-variant showed a significant association with risk of fluorosis. Pro-inflammatory cytokines (TNF-α, IL-1ß and IL-6) were found to be increased and have a significant correlation in response to fluoride exposure and cholinergic enzymes. The study concludes that chronic consumption of high fluoride-contained water is a risk factor for developing low-grade systemic inflammation through the cholinergic pathway and the studied cholinergic gene SNPs were identified to be associated with the risk of flurosis.

A novel mathematical model for COVID-19 with remedial strategies.

Coronavirus (COVID-19) outbreak from Wuhan, Hubei province in China and spread out all over the World. In this work, a new mathematical model is proposed. The model consists the system of ODEs. The developed model describes the transmission pathways by employing non constant transmission rates with respect to the conditions of environment and epidemiology. There are many mathematical models purposed by many scientists. In this model, " α E " and " α I ", transmission coefficients of the exposed cases to susceptible and infectious cases to susceptible respectively, are included. " δ " as a governmental action and restriction against the spread of coronavirus is also introduced. The RK method of order four (RK4) is employed to solve the model equations. The results are presented for four countries i.e., Pakistan, Italy, Japan, and Spain etc. The parametric study is also performed to validate the proposed model.

Pulmonary health risks of working at a cement plant in Khyber Pakhtunkhwa province of Pakistan.

OBJECTIVE: To examine the risk and preventive factors of working in a cement plant. METHODS: The cross-sectional study was conducted from July to September, 2016, at a leading cement manufacturing plant in the Khyber Pakhtunkhwa province of Pakistan. An interviewer-administered questionnaire was used to collect data about the characteristics of exposed and unexposed subjects. The characteristics and occupational illnesses were compared between exposed and unexposed workers. Logistic regression was applied to estimate the odds ratio for risk and preventive factors associated with pulmonary illnesses linked with the profession. RESULTS: Of the 550 workers contacted, 260(47.3%) agreed to participate. The mean age of the sample was 30.48}6.13 years. Of the total, 80(31%) were exposed and 180(69%) were unexposed. Overall, 96(37%) workers had medical coverage facility, while 114(63%) reported very good health. All socioeconomic and working characteristics were highly significant between exposed and unexposed workers (p<0.05). There were 67(25.7%) workers with pulmonary illnesses and the difference between the exposed and unexposed groups were significant (p<0.05). CONCLUSIONS: Pulmonary health among workers could be improved by implementing occupational health improving strategies..