RESUMEN
The outbreaks of hantavirus infections in some regions of the Russian Federation in some years involve considerable material and social losses. In this connection, the designing of the most effective types of vaccines is an urgent task. The authors have created plasmid constructions containing the gene of hemorrhagic fever with renal syndrome virus, with whose Intramuscular injection there is a specific immune response and plasmid DNA is detectable in the adjacent tissues within a month after injection.
Asunto(s)
Fiebre Hemorrágica con Síndrome Renal/prevención & control , Proteínas de la Nucleocápside/inmunología , Virus Puumala/inmunología , Vacunas de ADN/inmunología , Vacunas Virales/inmunología , Animales , Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/inmunología , Especificidad de Anticuerpos , Cartilla de ADN , Fiebre Hemorrágica con Síndrome Renal/inmunología , Inyecciones Intramusculares , Masculino , Ratones , Proteínas de la Nucleocápside/genética , Plásmidos/genética , Plásmidos/inmunología , Virus Puumala/genética , Vacunación , Vacunas de ADN/administración & dosificación , Vacunas de ADN/genética , Vacunas Virales/administración & dosificación , Vacunas Virales/genéticaRESUMEN
The "VectoHantivirus-ampli" test system based on rtPCR was shown as fitting the detection of virus RNA in blood samples of patients with hemorrhagic fever with renal syndrome (HFRS) made at early stages and no later than 7 days from the disease onset. A sequence analysis of viral nucleotide sequences of PCR products produced by the test-system ensured the identification of Hantaviruses (pathogens of HFRS). Two genetic variants of Puumula virus were shown to circulate in the territory of the Bashkortostan Republic; they differ by 10.0-13.8%, one of them is absolutely new. The Hantan virus FE genetic variant was detected in the studied samples from the Khabarovsk Territory.
Asunto(s)
Virus Hantaan/genética , Virus Hantaan/aislamiento & purificación , Fiebre Hemorrágica con Síndrome Renal/diagnóstico , Técnicas de Amplificación de Ácido Nucleico , Virus Puumala/genética , Virus Puumala/aislamiento & purificación , ARN Viral/análisis , Baskiria/epidemiología , Fiebre Hemorrágica con Síndrome Renal/epidemiología , Humanos , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Siberia/epidemiología , Factores de TiempoRESUMEN
Pumala virus recombinant nucleocapsid protein was used for the early diagnosis of haemorrhagic fever with renal syndrome. Specific IgM in the sera of patients could be determined by the IEA technique as early as on days 2-3 from the onset of the disease. The diagnostic effectiveness of the test-system was 95% and its specificity was 98%.
Asunto(s)
Fiebre Hemorrágica con Síndrome Renal/diagnóstico , Nucleocápside/inmunología , Virus Puumala/inmunología , Anticuerpos Antivirales/sangre , Escherichia coli/metabolismo , Vectores Genéticos , Fiebre Hemorrágica con Síndrome Renal/sangre , Humanos , Técnicas para Inmunoenzimas , Inmunoglobulina M/sangre , Nucleocápside/genética , Proteínas de la Nucleocápside , Proteínas Recombinantes/inmunología , Sensibilidad y Especificidad , Pruebas SerológicasRESUMEN
Fragments of the minor S and middle M segments of Hantavirus genome RNA were isolated by reverse transcription-polymerase chain reaction (RT-PCR) from the organs of patients who died from hemorrhagic fever with the renal syndrome (HFRS) during an epidemic outbreak of this infection in the Bashkortostan Republic in 1997-1998. The sizes of the resultant PCR fragments were 317 p. n. for S segment and 416 p. n. for M segment. Their primary structures (GENBANK registration numbers AJ133581 and AJ133582) were determined and they were compared with the nucleotide sequences of the respective genome sites of Hantavirus strain CG1820/Ufa-83 isolated from Clethrionomys in Bashkortostan in 1983. The homologies of nucleotide sequences of the corresponding sites of S and M segments of RNA isolate of 1997 and CG1820/Ufa-83 were 87.7 and 96.6%, respectively. The authors discuss the probability of circulation of hantaviruses differing by individual features of primary structure of genome RNA in Bashkortostan.
Asunto(s)
Fiebre Hemorrágica con Síndrome Renal/virología , Conformación de Ácido Nucleico , Orthohantavirus/genética , ARN Viral/química , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Chlorocebus aethiops , ADN Viral , Humanos , Datos de Secuencia Molecular , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Federación de Rusia , Células VeroRESUMEN
Animal experiments and trials on humans have shown that purified and concentrated chromatographic tissue-culture rabies vaccine is safe and essentially more potent than commercial tissue-culture rabies vaccine.