[Activity of certain dehydrogenases in accumulation and continuous-flow culture of paraffin-oxidizing yeast of the genus Candida]. / Aktivnost' nekotorykh degidrogenaz nakopitel'noi i nepreryvmo-protochnoi kul'tury parafinokisliaiushchikh drozhzhei roda Candida

Lability of NADP- and NAD-dependent glutamate dehydrogenases and malate dehydrogenases has been demonstrated during a change of the physiological activity of yeast Candida guilliermondii in the exponential phase of growth. Variations in the isoenzyme spectrum of the enzymes and total dehydrogenase activity of the yeast has been found during their transition from the accumulative to the continuous cultivation. Changes in the physiological state of yeast during their growth are accompanied by a rearrangement of the isoenzyme spectrum of malate dehydrogenase and glutamate dehydrogenases.

[Cytochromes of paraffin oxidizing yeast Candida guillermondii K-5]. / Tsitokhromy parafinokisliaiushchikh drozhzhei Candida guilliermondii K-5

The cytochrome group of the enzymes-electron carriers in the terminal site of the respiratory chain of paraffin-oxidizing yeast C. guilliermondii K-5 has been identified. Spectral peaks of their reduced form correspond to the following wave lengths: c-412, a1-510 and a2-546nm. Variations in the content of cytochrome C of the accumulative culture have been studied. Reduced forms are predominant in the lag-phase and in the phase of exponential growth. This predominance disappears in the phase with negative acceleration and the dying culturt contains a greater number of oxidized forms.

[Phosphorus metabolism in yeasts, oxidizing hydrocarbons during active aeration of the medium]. / Fosfornyi obmen u drozhzhei, okisliaiushchikh uglevodorody pri aktivnoi aératsii sredy

Variations in the phosphorus and nitrogen assimilation by hydrocarbon oxidizing yeast C. guilliermondii K-5 were followed upon an active aeration of the medium Changes in the phosphate forms of the acid-soluble and acid-insoluble fractions were found. By the end of the lag-phase the content of all phosphate forms, except stable polyphosphates, increased. At the onset of the active budding the content of labile phosphates, belonging to acid-soluble phosphates, increased. As the culture developed, the content of these forms decreased and increased slightly in the growth phase with a negative acceleration. Acid-insoluble polyphosphates were accumulated in the culture as long as it grew. In the growth phase with a negative acceleration these polyphosphates were hydrolyzed. This was responsible for an increment of all phosphates in the acid-soluble fraction. The same pattern occurred in the dying phase of the culture.