Comparative analysis of LC-MS/MS and real-time PCR assays for efficient detection of potential allergenic silkworm.

The silkworm (Bombyx mori) has long been valued food and feed in East Asia for its abundant nutritional and medicinal attributes, conversely, it can elicit allergic responses in susceptible individuals. Therefore, the development of silkworm detection method is required to avert allergenic incidents. In this study, two methodologies, tandem mass spectrometry (LC-MS/MS) and real-time PCR, were developed to achieve effective silkworm detection. These methods exhibited exceptional sensitivity in identifying silkworm presence in processed foods. Furthermore, model cookies spiked with silkworm were used to validate the sensitivities of LC-MS/MS (0.0005%) and real-time PCR (0.001%). Overall, these techniques were useful for trace silkworm detection in food products; therefore, they may help prevent allergic reactions. To the best of our knowledge, this study represents the first comparison of LC-MS/MS and real-time PCR methods for silkworm detection, marking an important contribution to the field. Data are available from ProteomeXchange under identifier PXD042494.

Rapid and Simultaneous Authentication of Six Laver Species Using Capillary Electrophoresis-Based Multiplex PCR.

Lavers are typically consumed in dried or seasoned forms. However, commercially processed lavers can lead to seafood fraud because it is impossible to authenticate the original species based on morphological characteristics alone. In this study, we developed a capillary electrophoresis-based multiplex polymerase chain reaction (PCR) to authenticate six different laver species. The species-specific primer sets to target the chloroplast rbcL or rbcS genes were newly designed. We successfully established both singleplex and multiplex conditions, which resulted in specific amplicons for each species (N. dentata, 274 bp; N. yezoensis, 211 bp; N. seriata, 195 bp; N. tenera, 169 bp; N. haitanensis, 127 bp; P. suborbiculata, 117 bp). Moreover, the assays were sensitive enough to detect DNA ranging from 10 to 0.1 pg of DNA. The optimized capillary electrophoresis-based multiplex PCR was successfully applied to 40 commercial laver products. In addition to detecting the laver species as stated on the commercial label, the assay discovered cases where less expensive species were mixed in. With its advantageous properties, such as short amplicon size, high specificity, and superior sensitivity, this assay could be used for the authentication of the six laver species.

Impact of Long-Term Care Service Quality on User Cooperation/Participation: Mediating Effect of Self-Determination.

The purpose of this study is to determine whether home care service quality affects users' voluntary cooperation/participation through self-determination. For this purpose, survey data from 358 long-term care service users in Korea were analyzed by applying a structural equation model. Data collection was conducted from February to March 2019. Data collection was conducted in February-March 2019. The results showed that home care quality did not directly affect users' voluntary cooperation/participation in the service delivery process, and self-determination fully mediated the relationship between quality and cooperation/participation. Based on these results, the importance of providing appropriate information, reflecting users' voices, and the attitude and role of experts was emphasized in order to promote user participation/cooperation through self-determination.

UAV aided virtual cooperative spectrum sensing for cognitive radio networks.

Cooperative spectrum sensing (CSS) involves multiple secondary users (SUs) reporting primary user (PU) channel sensing states to the fusion center (FC). However, the high overheads associated with multi-user CSS impose power limitations that limit its usefulness in unmanned aerial vehicle (UAV) networks. To address this challenge, we propose a virtual CSS, where a single UAV conducts CSS while following a circular flight trajectory in the air. The novelty of our approach is presenting a working frame structure for the UAV flight, including sensing and data transmission periods with further division of the sensing time into mini-sensing slots. In the virtual CSS, UAV performs local sensing decisions in each mini-slot and accumulates them for a final decision. The proposed virtual CSS scheme exploits sequential decision fusion (SDF), which sequentially adds individual mini-slot decisions. Additionally, we leverage machine learning (ML), employing AdaBoost ensembling classifier (ENC), to inspect flight conditions and reconfigure mini-slot periods dynamically for both traditional decision fusion (TDF) and our proposed SDF schemes. Furthermore, we identify an optimal decision threshold (ODT) for the proposed SDF, enabling the comparison of sequential results with an adjustable threshold through majority voting. This novel approach results in energy efficiency and improved throughput for virtual CSS using SDF, surpassing the performance of TDF, which relies on collecting entire mini-slot reports for its final decision. Simulation results demonstrate the effectiveness of the proposed SDF following the ENCODT (SDF-ENCODT) scheme compared to existing techniques from the literature. We explore varying levels of UAV flight velocities, moving radius, detection probability demand, and channel signal-to-noise ratio (SNR), reinforcing the significance of our contribution. Our research highlights the motivation to address spectrum scarcity in UAV communication by proposing an innovative virtual CSS scheme based on SDF. The proposed approach enhances spectrum utilization, overcomes power limitations, and substantially improves CSS for UAV networks.

Alpha-Defensin 1: An Emerging Periodontitis Biomarker.

Background: Research on the development of reliable diagnostic targets is being conducted to overcome the high prevalence and difficulty in managing periodontitis. However, despite the development of various periodontitis target markers, their practical application has been limited due to poor diagnostic accuracy. In this study, we present an improved periodontitis diagnostic target and explore its role in periodontitis. Methods: Gingival crevicular fluid (GCF) was collected from healthy individuals and periodontitis patients, and proteomic analysis was performed. The target marker levels for periodontitis were quantified in GCF samples by enzyme-linked immunosorbent assay (ELISA). Mouse bone marrow-derived macrophages (BMMs) were used for the osteoclast formation assay. Results: LC-MS/MS analysis of whole GCF showed that the level of alpha-defensin 1 (DEFA-1) was higher in periodontitis GCF than in healthy GCF. The comparison of periodontitis target proteins galactin-10, ODAM, and azurocidin proposed in other studies found that the difference in DEFA-1 levels was the largest between healthy and periodontitis GCF, and periodontitis was more effectively distinguished. The differentiation of RANKL-induced BMMs into osteoclasts was significantly reduced by recombinant DEFA-1 (rDEFA-1). Conclusions: These results suggest the regulatory role of DEFA-1 in the periodontitis process and the relevance of DEFA-1 as a diagnostic target for periodontitis.

Impact of peripheral alkyl chain length on mesocrystal assemblies of G2 dendrons.

Unique sphere-packing mesophases such as Frank-Kasper (FK) phases have emerged from the viable design of intermolecular interactions in supramolecular assemblies. Herein, a series of Cn-G2-CONH2 dendrons possessing an identical core wedge are investigated to elucidate the impact of peripheral alkyl chain lengths (Cn) on the formation of the close-packed structures. The C18 and C14 dendrons, of which the contour lengths of the periphery Lp are longer than the wedge length Lw, assemble into a uniform sphere-packing phase such as body-centred cubic (BCC), whereas the C8 dendron with short (Lp < Lw) corona environment forms the FK A15 phase. Particularly in the intermediate C12 and C10 dendrons (Lp ≈ Lw), cooling the samples from an isotropic state leads to cooling-rate-dependent phase behaviours. The C12 dendron produces two structures of hexagonal columnar and sphere-packing phases (BCC and A15), while the C10 dendron generates the A15 and σ phases by the fast- and slow-cooling processes, respectively. Our results show the impact of peripheral alkyl chain lengths on the formation of mesocrystal phases, where the energy landscape of the dendrons at Lp/Lw ≈ 1 must be more complex and delicate than those with either longer or shorter peripheral alkyl chains.

Golf-related sports injuries of the musculoskeletal system.

The purpose of this study was to evaluate the characteristics of golf-related musculoskeletal injuries. Golfers were retrospectively surveyed through a 50-item questionnaire. Golfers were divided into injured and noninjured groups according to the presence or absence of musculoskeletal injuries. Of the 208 respondents, 64.0% suffered golf-related injuries, most of which were minor injuries caused by overuse in the early 9.9 weeks. According to the anatomical distribution, the most injured parts were upper limbs, spine, trunk, and lower limbs in that order. As for the distribution by part, the hand and wrist were the most vulnerable, and the lateral elbow damage was 3 times more common than the inner elbow. Self-management for injuries was the most common treatment, and regular preventive exercise accounted for 30% of the injured. Overall, more than half of the respondents suffered from musculoskeletal injuries, self-management was the most common, and less than one-third did regular exercise to prevent injuries. Therefore, active management to prevent golf-related musculoskeletal injuries may be necessary.

Effect of bodybuilding athletes' weight loss method on performance factors and immune function.

This study was conducted on elite bodybuilders for 6 weeks using two weight loss methods (traditional method=high-weight loss vs. new method=low-weight loss). The subjects of this study were 26 male active elite bodybuilders who voluntarily wanted to participate in this experiment, and were divided into experimental group and traditional group. The traditional and experimental groups underwent dietary restrictions and training for 6 weeks. The conclusion obtained from the results of this study is that in the case of anthropometric variables, there is a difference in weight class between the two groups. The experimental group's upper arm circumference was maintained compared to the pre-measured value after post-measurement, while the traditional group decreased, and a significant interaction effect was observed. In particular, a significant interaction effect was observed. In that the absolute value of maximum oxygen intake was significantly increased only in the post-experimental group compared to the pretest, and an interaction effect was observed, the use of low-weight loss method is more effective than the traditional method of high-weight loss method. An increase in some cytokines was observed despite traditional weight loss, but it did not cause an excessive decrease in immunity or a sharp decrease in performance factors. As a result of this experiment, it is judged that the use of a low-weight loss method is more preferable than the traditional high-weight loss method in relation to performance variables.

The effects of workers' job stress on organizational commitment and leaving intention in commercial sports centers.

The purpose of this study is to identify the factors affecting the job stress of commercial sports center workers on organizational commitment and turnover intention. The causal relationship between their demographic characteristics and job stress was investigated for 261 out of 300 workers working at commercial sports centers in Seoul, Incheon, and Gyeonggi province. There was a difference by work type, and it was found that there was a difference between regular and contract workers. Role-related and interpersonal relationships had a negative influence relationship in terms of tenure commitment, normative commitment, and affective commitment. Job characteristics, role-related, interpersonal relationship, and compensation system had an influence on turnover intention. In other words, it was found that role-related and interpersonal relationships had a positive effect on turnover intention, and job characteristics and compensation system had a negative effect on turnover intention. It was found that job stress affects both organizational commitment and turnover intention. The results of this study indicate that job stress, organizational commitment, and turnover intention should be dealt with mainly in order to reduce turnover intention of commercial sports center workers. In other words, clearer guidelines on the role of commercial sports center workers and various welfare programs for improving human relationships should be provided. Therefore, commercial sports centers should continuously research and develop ways to maximize job satisfaction from the perspective of workers in order to reduce job stress and induce positive organizational commitment.

Effect of chronic alcohol intake on the expression of muscle atrophy-related proteins in growing rats.

In this study, the effect of chronic alcohol intake for 4 weeks on the muscular atrophy factors of rat skeletal muscle was studied using 6-week-old growing Sprague-Dawley rats. Experimental animals were classified into a control group and an alcohol intake group. The alcohol intake group consumed alcohol orally at a concentration of 3-g/kg body weight every day for 4 weeks. The control group consumed tap water in the same way. After 4 weeks alcohol ingestion, glucose, total cholesterol, triglyceride, and high-density lipoprotein cholesterol in serum levels were measured. Western blot was performed to detect the expressions of muscle RING-finger protein-1 (MuRF1), protein kinase B (Akt), phosphorylated Akt (p-Akt), forkhead box O (FoxO), phosphorylated FoxO (p-FoxO), p38, and phosphorylated p38 (p-p38). Results of this experiment showed that chronic alcohol intake enhanced triglyceride concentration. Chronic alcohol intake increased MuRF1 expression to promote muscle proteolysis and decreased p-Akt/Akt ratio and p-FoxO/FoxO ratio to inhibit skeletal muscle growth. Therefore, alcohol consumption has been shown to cause muscle atrophy.

Control of crystallization and magnetic properties of CoFeB by boron concentration.

Controlling the crystallinity of CoFeB is the most essential issue for designing various spintronics devices. Here we show the microstructure and magnetic properties of MgO/CoFeB/MgO structures for various boron concentration. We present the effect of boron on the crystallinity of CoFeB into two categories: the critical boron concentration (5 ~ 6%) at which CoFeB crystallizes and the effect of remaining boron (0 ~ 5%) in the crystallized CoFeB. And the trends of the saturation magnetization, exchange stiffness, exchange length, domain wall energy and Gilbert damping constant according to the boron concentration are provided. Abrupt variation of properties near the critical boron concentration (5 ~ 6%) and a noticeable change in the crystallized CoFeB (0 ~ 5%) are confirmed, revealing a clear causal relationship with the structural analysis. These results propose that the crystallization, microstructure, and major magnetic properties of CoFeB are governed by the amount of boron, and emphasize the need for delicate control of boron concentration.

Self-esteem and social development according to participation in school sports club.

The purpose of this study is to explore the meaning of the middle school sports club and to understand the impact on the self-esteem and social development of middle school students participating in the school sports club. To achieve the purpose of this study, a questionnaire survey was conducted on 450 students by selecting a middle school. Of the 420 collected questionnaires, 399 questionnaires were used as a valid sample. As a result of examining seven areas of self-esteem, self-esteem in five areas excluding domestic ego and personality ego was highest in the group that participated in the league. As a result of examining the difference in social development according to the type of participation in school sports clubs, the league participation group was higher in all five areas. As a result of examining the difference in self-esteem according to the period of participation in school sports clubs, the group participating in the league was high in six areas excluding physical ability, however, it was difficult to see a statistically significant difference. As for the factor related to physical ability, the group with the longest participation period of 13 months or longer was the highest, and there is a statistically significant difference. As a result of examining the difference in social development according to the period of participation in school sports clubs, the group under 6 months was the highest in four areas other than physical activity. Physical activity was highest in the group over 13 months.

Effect of exercise immersion experience on health promotion and lifelong physical education of high school students in sports club activities.

The purpose of this study was to investigate the impact of high school students' athletic commitment, health promotion education, and lifelong sports activities. To this end, the researcher searched for research subjects of 397 high school students residing in Seoul in 2019. As a result of the analysis, the following conclusions were drawn. First, the effect of exercise commitment on sports health promotion education was investigated. Looking at the activities of high school students, their commitment to behavior has had a profound impact on their health responsibilities and relationships. Second, the study also investigated the impact of athletic commitment to lifelong sports of sports activities in high school students and found that cognitive and behavioral commitment had a significant impact on lifelong sports. Finally, as a result of investigating the impact of health promotion education on lifelong sports of high school student sports activities, it was found that health responsibility and relationships have a great influence on lifelong sports.

Effects of Kinesio taping on blood fatigue factors after isokinetic exercise.

This study examined the effects of Kinesio taping on recovery from fatigue induced by an exercise of concentric contraction using an isokinetic machine. Eight healthy collegiate students participated in two experiments: the Kinesio taping application condition and the no Kinesio taping application condition. The fatigue was induced by concentric exercise at 60°/sec, 50 repetitions for one session, and repeated 3 sessions. Changes of blood ammonia, lactate, lactate dehydrogenase (LDH), and creatinine kinase (CK) were monitored. Blood was collected before exercise, immediately after exercise, 24 hr after exercise, and 72 hr after exercise. Blood ammonia tended to reduce during the recovery process, but no differences were found between conditions. Blood lactate tended to reduce during the recovery process, but no differences were found between conditions. In the blood LDH, no differences were found between conditions. Blood creatine kinase tended to reduce during the recovery process, but no differences were found between conditions. The present results showed that Kinesio taping did not affect the recovery phase of blood ammonia, lactate concentration, LDH, and CK.

Swimming Exercise Ameliorates Symptoms of MOG-Induced Experimental Autoimmune Encephalomyelitis by Inhibiting Inflammation and Demyelination in Rats.

PURPOSE: Multiple sclerosis is an autoimmune disease that affects the central nerve system, resulting in cumulative loss of motor function. Multiple sclerosis is induced through multiple mechanisms and is caused by inflammation and demyelination. This study aims to evaluate the neuroprotective effect of swimming exercise in experimental autoimmune encephalomyelitis (EAE) rats, an animal model of multiple sclerosis. METHODS: EAE was induced by an intradermal injection of 50-µg purified myelin oligodendrocyte glycoprotein 33-55 (MOG33-55) dissolved in 200-µL saline at the base of the tail. The rats in the swimming exercise group were made to swim for 30 minutes once pert a day for 26 consecutive days, starting 5 days after induction of EAE. To compare the effect of swimming exercise with interferon-ß, a drug for multiple sclerosis, interferon-ß was injected intraperitoneally into rats of the EAE-induced and interferon-ß-treated group during the exercise period. RESULTS: Injection of MOG33-55 caused weight loss, decreased clinical disability score, and increased level of pro-inflammatory cytokines and inflammatory mediators in the lumbar spinal cord. Loss of motor function and weakness increased demyelination score. Swimming exercise suppressed demyelination and expression of pro-inflammatory cytokines and inflammatory mediators. These changes promoted recovery of EAE symptoms such as body weight loss, motor dysfunction, and weakness. Swimming exercise caused the same level of improvement as interferon-ß treatment. CONCLUSION: The results of this experiment suggest the possibility of swimming exercise in urological diseases that are difficult to treat. Swimming exercises can be considered for relief of symptom in incurable multiple sclerosis.

Effects of whole-body vibration exercise on functions required for bowling performance of male bowling player.

The focus of this research is to examine the impact of 8 weeks of whole body vibration (WBV) exercise on physical factors by conducting an experiment on 17 male bowling players registered in Korea Bowling Association. Strength of truncus and lower-limb and balance were mea-sured as the physical factors and the impact of WBV exercise on each variable was examined. There was significant increase between groups in flexor-weight ratio of truncus at 60°/sec according to performance after applying WBV exercise. There was significant difference in groups before bowling event in pre and posttest as well as after bowling event in pre and posttest. There was significant difference of extensor-weight ratio in groups after bowling event in pre and posttest. There was significant increase at 60°/sec in flexor-ratio of both left and right lower-limb strength according to performance after applying WBV exercise. There was significant difference between groups before bowling event in pre and posttest as well as after bowling event in pre and posttest. Extensor-weight ratio showed significant difference between groups only before bowling event in pre and posttest. There was significant difference between groups in both left and right balance according to performance after applying WBV exercise. There was significant difference in before bowling event in pre and posttest as well as after bowling event in pre and posttest.

Spray-dried plasma attenuates inflammation and lethargic behaviors of pregnant mice caused by lipopolysaccharide.

This study evaluated whether dietary spray-dried plasma (SDP) can ameliorate inflammation, lethargic behaviors, and impairment of reproduction caused by lipopolysaccharide (LPS) challenge during late pregnancy. Two experiments were conducted with 125 mated female mice (C57BL/6 strain) in each experiment. All mice were shipped from a vendor on the gestation day (GD) 1 and arrived at the laboratory on GD 3. Mice were randomly assigned to dietary treatments with or without 8% SDP in the diet. On GD 17, mice determined pregnant by BW and abdomen shape were randomly assigned to intraperitoneal injections with or without 2 µg LPS. In experiment 1, 17 mice (26.7 ± 1.7 g BW) were identified pregnant and euthanized 6 h after the LPS challenge to measure inflammatory responses in uterus and placenta. In experiment 2, 44 mice (26.0 ± 1.6 g BW) were identified pregnant and euthanized 24 h after the LPS challenge to assess behavior and late-term pregnancy loss. Growth performance and reproductive responses, such as loss of pregnancy, percentage of fetal death, and etc., were measured in all pregnant mice. The LPS challenge increased (P < 0.05) uterine and placental tumor necrosis factor-α and interferon-γ, late-term pregnancy loss, and lethargy score, and decreased (P < 0.05) uterine transforming growth factor-ß1, moving time and number of rearing, and growth and feed intake. The SDP decreased (P < 0.05) concentrations of both pro-inflammatory and anti-inflammatory cytokines in one or both tissues, and the lethargy score, and increased (P < 0.05) moving time and number of rearing, growth of pregnant mice, and fetal weight. However, the SDP did not affect late-term pregnancy loss caused by the LPS challenge. Consequently, dietary SDP attenuated acute inflammation and lethargic behaviors of pregnant mice caused by the LPS challenge, but did not affect late-term pregnancy loss after the acute inflammation.

Effects of different gestation housing types on reproductive performance of sows.

This study evaluated the effects of different gestation housing types on reproductive performance of sows. A total of 60 sows (218 ± 24 kg body weight) with mixed parity were used. During gestation, 28 sows were housed in groups with electronic sow feeders (space allowance = 1.26 m2 /sow) and 32 sows were housed in individual stalls (space allowance = 1.20 m2 /sow). Sows from both housing types were moved to farrowing crates on day 109 of gestation and stayed until weaning (18 days post-farrowing). Typical corn-soybean meal diets were provided to sows during gestation and lactation. Measurements were reproductive performance of sows at farrowing as well as performance of sows and their litter during lactation. Similar total numbers of piglets born at farrowing were observed for sows gestated in both housing types. However, group-housed gestation sows had more mummies (0.321 vs. 0.064; P < 0.05) and stillbirths (0.893 vs. 0.469; P = 0.073) at farrowing than individual-housed gestation sows. Consequently, individual-housing type had higher percentage of piglets born alive (95.5 vs. 90.4%; P < 0.05) than the group-housing type. Therefore, improved reproductive performance of sows from individual gestating housing was confirmed in this study.

Correction: The Effect of Lifestyle Changes on Blood Pressure Control among Hypertensive Patients.

[This corrects the article on p. 173 in vol. 38, PMID: 28775806.].

The Effect of Lifestyle Changes on Blood Pressure Control among Hypertensive Patients.

BACKGROUND: Hypertension is highly prevalent among patients who visit primary care clinics. Various factors and lifestyle behaviors are associated with effective blood pressure control. We aimed to identify factors and lifestyle modifications associated with blood pressure control among patients prescribed antihypertensive agents. METHODS: This survey was conducted at 15 hospital-based family practices in Korea from July 2008 to June 2010. We prospectively recruited and retrospectively assessed 1,453 patients prescribed candesartan. An initial evaluation of patients' lifestyles was performed using individual questions. Follow-up questionnaires were administered at 4, 8, and 12 weeks. We defined successful blood pressure control as blood pressure <140 mm Hg systolic and <90 mm Hg diastolic. RESULTS: Of the 1,453 patients, 1,139 patients with available data for initial and final blood pressures were included. In the univariate analysis of the change in performance index, weight gain (odds ratio [OR], 2.18; 95% confidence interval [CI], 1.52 to 3.11; P<0.001), physical inactivity (OR, 1.195; 95% CI, 1.175 to 3.387; P=0.011), and increased salt intake (OR, 1.461; 95% CI, 1.029 to 2.075; P=0.034) were related to inadequate blood pressure control. Salt intake also showed a significant association. Multivariate ORs were calculated for age, sex, body mass index, education, income, alcohol consumption, smoking status, salt intake, comorbidity, and family history of hypertension. In the multivariate analysis, sex (OR, 3.55; 95% CI, 2.02 to 6.26; P<0.001), salt intake (OR, 0.64; 95% CI 0.43 to 0.97; P=0.034), and comorbidity (OR, 1.82; 95% CI, 1.23 to 2.69; P=0.003) were associated with successful blood pressure control. CONCLUSION: Weight gain, physical inactivity, and high salt intake were associated with inadequate blood pressure control.